Exploring the Interactive Impacts of Citronellol, Thymol, and Trans-Cinnamaldehyde in Broilers: Moving towards an Improved Performance, Immunity, Gastrointestinal Integrity, and Clostridium perfringens Resistance.

AIMS: This study aimed to explore the effectiveness of dietary citronellol, thymol, and trans-cinnamaldehyde (CTC) essential oils blend on broilers` growth performance, immunity, intestinal microbial count, gut integrity, and resistance against Clostridium perfringens utilizing the necrotic enteritis (NE) challenge model. METHODS AND RESULTS: A total of 200 Ross 308 male broiler chicks received either a control diet or diet supplemented with three graded levels of CTC blend including 300, 600, and 900 mg of CTC blend/Kg diet and experimentally infected with C. perfringens strain at 23 days of age. Herein, dietary CTC blend fortifications significantly improved the broilers` growth performance, which was supported by upregulating the expression levels of MUC-2, occludin, and JAM-2 genes. Moreover, dietary CTC blend inclusion significantly enhanced the levels of blood phagocytic percentage and serum IgA, IgG, and MPO, and reduced the values of serum CRP, and NO at 5 days pre-infection, 10-, and 15 days post-infection (dpi) with C. perfringens. At 15 dpi, CTC blend inclusion significantly reduced the intestinal digesta pH, coliforms and C. perfringens loads, and the expression levels of genes related to C. perfringens virulence (cpe, cnaA, and nanI), proinflammatory cytokines (IL-1ß and TNF-α), and chemokines (CCL20), in addition to increasing the count of beneficial total Lactobacillus and total aerobic bacteria, and the expression levels of genes related to anti-inflammatory cytokines (IL-10) and chemokines (AvBD6, and AvBD612). CONCLUSION: Our results point to the growth-provoking, immunostimulant, antibacterial, anti-inflammatory, and antivirulence characteristics of the CTC blend, which improves the broilers' resistance to C. perfringens and ameliorates the negative impacts of NE.

Comparison of different dietary sources of n-3 polyunsaturated fatty acids on immune response in broiler chickens.

The study aims to research the effects of varied dietary sources of n-3 polyunsaturated fatty acids (PUFA) on the immune response in broiler chickens with stress on natural killer (NK) cell activity. Diets supplemented with one of the four sources of n-3 PUFA: linseed oil-, echium oil-, fish oil (FO) or algal biomass-enriched diets at levels of 18, 18, 50 and 15 g/kg fresh weight, were provided for one-d-old male Ross 308 broilers, totaling 340 in number, until they were slaughtered. The analyses included total lipid profile using gas chromatography (GC) for plasma, spleen, thymus, and blood. Additionally, NK cell activity and cell proliferation were investigated for thymocytes and splenocytes. The results indicated that the source of n-3 PUFA had a strong influence on fatty acid composition across all tissues. NK activity was highest in splenocytes and PBMCs from broilers fed linseed oil, followed by those fed algal biomass or echium oil, and lowest for those from broilers fed FO. The proliferative response of lymphocytes from algal biomass-fed chickens tended to be the highest, followed by those fed linseed oil in most cases. Lymphocytes from chickens fed fish oil showed the lowest proliferative response. These results could mean that a docosahexaenoic acid (DHA)-rich algal product might enrich chicken meat with n-3 PUFA without significant damaging effects on chicken immunity.

Effect of Different Levels of Multienzymes on Immune Response, Blood Hematology and Biochemistry, Antioxidants Status and Organs Histology of Broiler Chicks Fed Standard and Low-Density Diets.

This study was executed to investigate the effect of supplementing three multienzyme levels (0, 0. 1, and 0.2%) with two types of diet [standard diet (SD) vs. low-density diet (LDD)] on immune response, blood hematology and biochemistry, antioxidant status, and organ histology of broilers during 1-38 days of age. A total of 216 unsexed 1-day-old Arbor Acres broiler chicks were randomly distributed, on a factorial design (2 × 3), to six treatments each with six replicates. There were six chicks per replicate. Results showed that LDD significantly decreased body weight gain (BWG) of broilers, but did not affect the European Production Efficiency Index (EPEI). Addition of multienzymes at both levels (0.1 and 0.2%) significantly increased BWG and improved EPEI, compared to the control diet. Alanine aminotransferase (ALT), aspirate aminotransferase (AST), malondialdehyde (MDA), lymphocyte, lymphocyte transformation test (LTT), and phagocyte activity (PA) were significantly higher for LDD than the SD, but eosinophil was lower. Supplementation of multienzymes significantly decreased ALT, AST, and MDA, compared to the control group, but increased packed cell volume (PCV), hemoglobin (Hgb), lymphocytes, and monocytes. Immune organs, such as spleen, thymus, and the bursa of Fabricius were significantly increased with multienzyme supplementation. It could be concluded that multienzyme supplementation at either 0.1 or 0.2% to SD or LDD improved EPEI and immune status of broiler chicks.

Benefits of probiotics and/or prebiotics for antibiotic-reduced poultry.

Antibiotics have been used for many years as growth promoters. They contribute to build the immunocompetence (i.e. ability of the body to produce a normal immune response following exposure to an antigen) of birds against infectious diseases and as growth promoters. Antibiotics have been widely used as growth promoters in the field of animal production since 1940s. There is a hypothesis that is effect is brought about by dynamic biological interaction with the micro-flora in the intestine. In 1951, the United States Food and Drug Administration approved the use of antibiotics as animal additives to prevent disease in general and, in some cases, to improve efficiency without veterinary prescription. In the 1950s and 1960s, each European state approved its own national regulations about the use of antibiotics in animal feeds. However, using antibiotics may develop bacteria resistant to these drugs. Accordingly, the use of antibiotics has been minimized and replaced by effective dietary supplements such as probiotics and/or prebiotics that are claimed to enhance growth and positively modulate the immune response. The current review paper sheds light on the benefits of using probiotics and/or prebiotics in poultry feed versus the risk of using antibiotics and the mechanisms by which they exert their effects, as well as the economic analysis of using these beneficial additives in poultry feed.

Blood Hematological and Biochemical Constituents, Antioxidant Enzymes, Immunity and Lymphoid Organs of Broiler Chicks Supplemented with Propolis, Bee Pollen and Mannan Oligosaccharides Continuously or Intermittently.

This study investigate the effect of bee pollen (BP) and/or propolis (Pro) as an alternative to Mannan oligosaccharides (Bio-MOS, a hydrolyzed yeast with natural and artificial flavors Alltech Inc) when given continuously or intermittently on antioxidant enzymes, immunity, weight and morphology of lymphoid organs of broilers. Thus, 324 unsexed one-day-old Arbor Acres broilers were randomly distributed into nine treatment groups, each replicated 6 times of 6 birds per replicate. The chicks were kept in wire cages and fed the same basal diet and were submitted to the following treatments: control without supplementation (control) or supplemented with BP at 300 mg, Pro at 300 mg, BP+Pro at 300 mg each and Bio-MOS at 0.5 g/kg diet. Each supplemented group was subdivided into two subgroups in which the additives were supplied continuously or intermittently. In the continuously supplemented groups, supplementations were given from one till 36 days of age, and in the intermittently supplemented groups, the administration was only three days before, on the day of and day after vaccination. The BP and Pro supplied continuously or intermittently was equally potent for improving immunity, antioxidant enzymes similar to Bio-MOS. All supplements supplied either continuously or intermittently resulted a significantly higher thymus (P < .04) and bursa weights (P < .001) than the control group. Combining BP with Pro resulted in a further increase in thymus weights and small follicle diameter compared to the control group. In addition, thymus percentage in the group received BP+Pro showed a further increase compared to the control and Pro supplemented intermittently. Bio-MOS, when supplied continuously or intermittently, resulted in the greatest response in splenic lymphoblasts. Supplementation with either BP or Pro intermittently, is adequate to promote health and immune response of broiler chicks, with 40% saving of supplementation costs.