Molecular characterization of Brazilian FeLV strains in São Luis, Maranhão Brazil.

The feline leukemia virus (FeLV) belongs to the Retroviridae family and Gammaretrovirus genus, and causes a variety of neoplastic and non-neoplastic diseases in domestic cats (Felis catus), such as thymic and multicentric lymphomas, myelodysplastic syndromes, acute myeloid leukemia, aplastic anemia, and immunodeficiency. The aim of the present study was to carry out the molecular characterization of FeLV-positive samples and determine the circulating viral subtype in the city of São Luís, Maranhão, Brazil, as well as identify its phylogenetic relationship and genetic diversity. The FIV Ac/FeLV Ag Test Kit (Alere™) and the commercial immunoenzymatic assay kit (Alere™) were used to detect the positive samples, which were subsequently confirmed by ELISA (ELISA - SNAP® Combo FeLV/FIV). To confirm the presence of proviral DNA, a polymerase chain reaction (PCR) was performed to amplify the target fragments of 450, 235, and 166 bp of the FeLV gag gene. For the detection of FeLV subtypes, nested PCR was performed for FeLV-A, B, and C, with amplification of 2350-, 1072-, 866-, and 1755-bp fragments for the FeLV env gene. The results obtained by nested PCR showed that the four positive samples amplified the A and B subtypes. The C subtype was not amplified. There was an AB combination but no ABC combination. Phylogenetic analysis revealed similarities (78% bootstrap) between the subtype circulating in Brazil and FeLV-AB and with the subtypes of Eastern Asia (Japan) and Southeast Asia (Malaysia), demonstrating that this subtype possesses high genetic variability and a differentiated genotype.

Encefalite parasitária em cadela / Parasitic encephalitis in a bitch

Background: Encephalitis is common in most inflammatory and infectious diseases of the central nervous system, andbacterial and viral pathogens, protozoa, fungi, and erythematous parasites are recognized as etiological agents. Clinicalsigns vary according to the anatomical location and severity of the inflammation. Some parasites may undergo aberrantmigration into the brain of dogs and cats, which is are considered a rare clinical phenomenon. This case report describesthe occurrence of parasitic encephalitis in a mixed breed bitch about 4 year-old.Case: A mixed breed bitch, about 4 year-old, rescued from the street with weakness of the pelvic limbs, was treated at theVeterinary Hospital of the Federal University of Piauí - UFPI. After A clinical and neurological examination, the patientwas hospitalized for diagnosis, treatment, confirmation and follow-up of her clinical evolution. Complementary tests wereperformed to evaluate her general condition, given her unknown history because she was a rescue dog. Complete blood count(CBC), hemoparasite screening, biochemical function (urea, creatinine, ALT, alkaline phosphatase, total protein, albuminand globulin), abdominal and pelvic ultrasound, serological and parasitological tests for leishmaniasis, distemper antigenand ehrlichiosis antibody detection tests were performed, and cerebrospinal fluid (CSF) was collected for analysis. TheCBC revealed normocytic hypochromic anemia and thrombocytopenia. Biochemical functions were within normal limits,except for alanine aminotransferase (ALT), which was slightly low. The ultrasound examination showed splenomegaly.The canine distemper, ehrlichiosis and leishmaniasis tests were negative. The results of the CSF analysis revealed the presence of spherical structures compatible with parasitic cysts, suggestive of Echinococcus granulosus. After the laboratorydiagnosis of parasitic encephalitis, the animal was treated with prednisolone...(AU)

Encefalite parasitária em cadela / Parasitic encephalitis in a bitch

Background: Encephalitis is common in most inflammatory and infectious diseases of the central nervous system, andbacterial and viral pathogens, protozoa, fungi, and erythematous parasites are recognized as etiological agents. Clinicalsigns vary according to the anatomical location and severity of the inflammation. Some parasites may undergo aberrantmigration into the brain of dogs and cats, which is are considered a rare clinical phenomenon. This case report describesthe occurrence of parasitic encephalitis in a mixed breed bitch about 4 year-old.Case: A mixed breed bitch, about 4 year-old, rescued from the street with weakness of the pelvic limbs, was treated at theVeterinary Hospital of the Federal University of Piauí - UFPI. After A clinical and neurological examination, the patientwas hospitalized for diagnosis, treatment, confirmation and follow-up of her clinical evolution. Complementary tests wereperformed to evaluate her general condition, given her unknown history because she was a rescue dog. Complete blood count(CBC), hemoparasite screening, biochemical function (urea, creatinine, ALT, alkaline phosphatase, total protein, albuminand globulin), abdominal and pelvic ultrasound, serological and parasitological tests for leishmaniasis, distemper antigenand ehrlichiosis antibody detection tests were performed, and cerebrospinal fluid (CSF) was collected for analysis. TheCBC revealed normocytic hypochromic anemia and thrombocytopenia. Biochemical functions were within normal limits,except for alanine aminotransferase (ALT), which was slightly low. The ultrasound examination showed splenomegaly.The canine distemper, ehrlichiosis and leishmaniasis tests were negative. The results of the CSF analysis revealed the presence of spherical structures compatible with parasitic cysts, suggestive of Echinococcus granulosus. After the laboratorydiagnosis of parasitic encephalitis, the animal was treated with prednisolone...

Imagem Ultrassonográfica de Folículo Ovariano de Kinosternon scorpioides (LINNAEUS, 1766), em diferentes fases do desenvolvimento / Image sonographic of follicle of female Kinosternon scorpoides (LINNAEUS, 1766)

tThe species Kinosternon scopioides, is a small freshwater turtle foun in the region known as BaixadaMaranhense, Maranhão, Brazil.. As studies on the species are still in the research process, the aim of thisscientific article was analyze the female reproductive organ through the ultrasonography to create a standart forimaging tests in this species and gain knowledge abaut their reproductive biology. For this, we used two femaleof Kinosternon scopioides where ultrasounds were performed twice a week. It was found thatgonads havefollicles in different stages of development and it was possible to distinguish follicles from eggs. The study showsimportant to help veterinary wildlife, and the preservation of the species, since the knowledge of reproductivecycle will help in captive breeding of them.(AU)

Avaliação andrológica de ovinos do Núcleo de Ruminantes da UEMA Campus Paulo VI / Evaluation andrologic of Ruminant Center of cattle from UEMA - Campus Paulo VI

The aim of the study was to characterize andrologicaly sheeps belonging to Ruminants Center ofUniversidade Estadual do Maranhao. The semen collection was performed by electro ejaculation. The bodycondition score from seven animals showed averaging 4.57 ± 1.13. The scrotal circumference (SC) was 30.58 ±4.91 cm, a scrotal circumference of rams presented mean length and width of the right testicle of 9.43 ± 1.16 cmand 5.44 ± 0.46 cm and the left testicle of 9.0 ± 0.81 cm and 5.51 ± 0.41 cm, respectively. The average volume ofsemen obtained was 0.89 ± 0.32 mL, appearance, color and odor sui generis were for the species. For physicalsemen parameters, the average turbulence was 3.57 ± 0.53; force of 3.14 ± 0.38; Sperm motility of 81.43 ±6.90%; sperm concentration of 0.8 ± 0.18 x109sptz / mL. The proportion of living and dead sperm stained witheosin-nigrosin averaged 74.1 ± 3.02% and 25 ± 3.46%, respectively. According to this study and the resultsobtained, sheep showed morphology and physical aspects consistent with the species and can be used asbreeders.(AU)

Imagem Ultrassonográfica de Folículo Ovariano de Kinosternon scorpioides (LINNAEUS, 1766), em diferentes fases do desenvolvimento / Image sonographic of follicle of female Kinosternon scorpoides (LINNAEUS, 1766)

tThe species Kinosternon scopioides, is a small freshwater turtle foun in the region known as BaixadaMaranhense, Maranhão, Brazil.. As studies on the species are still in the research process, the aim of thisscientific article was analyze the female reproductive organ through the ultrasonography to create a standart forimaging tests in this species and gain knowledge abaut their reproductive biology. For this, we used two femaleof Kinosternon scopioides where ultrasounds were performed twice a week. It was found thatgonads havefollicles in different stages of development and it was possible to distinguish follicles from eggs. The study showsimportant to help veterinary wildlife, and the preservation of the species, since the knowledge of reproductivecycle will help in captive breeding of them.

Avaliação andrológica de ovinos do Núcleo de Ruminantes da UEMA – Campus Paulo VI / Evaluation andrologic of Ruminant Center of cattle from UEMA - Campus Paulo VI

The aim of the study was to characterize andrologicaly sheeps belonging to Ruminants Center ofUniversidade Estadual do Maranhao. The semen collection was performed by electro ejaculation. The bodycondition score from seven animals showed averaging 4.57 ± 1.13. The scrotal circumference (SC) was 30.58 ±4.91 cm, a scrotal circumference of rams presented mean length and width of the right testicle of 9.43 ± 1.16 cmand 5.44 ± 0.46 cm and the left testicle of 9.0 ± 0.81 cm and 5.51 ± 0.41 cm, respectively. The average volume ofsemen obtained was 0.89 ± 0.32 mL, appearance, color and odor sui generis were for the species. For physicalsemen parameters, the average turbulence was 3.57 ± 0.53; force of 3.14 ± 0.38; Sperm motility of 81.43 ±6.90%; sperm concentration of 0.8 ± 0.18 x109sptz / mL. The proportion of living and dead sperm stained witheosin-nigrosin averaged 74.1 ± 3.02% and 25 ± 3.46%, respectively. According to this study and the resultsobtained, sheep showed morphology and physical aspects consistent with the species and can be used asbreeders.