RESUMEN
AIM: Breath analyses have potential to detect early signs of disease onset. Ambient ionization allows direct combination of breath gases with MS for fast, on-line analysis. Portable MS systems would facilitate field/clinic-based breath analyses. Results & methodology: Volunteers ingested peppermint oil capsules and exhaled volatile compounds were monitored over 10 h using a compact mass spectrometer. A rise and fall in exhaled menthone was observed, peaking at 60-120 min. Real-time analysis showed a gradual rise in exhaled menthone postingestion. Sensitivity was comparable to established methods, with detection in the parts per trillion range. CONCLUSION: Breath volatiles were readily analyzed on a portable mass spectrometer through a simple inlet modification. Induced changes in exhaled profiles were detectable with high sensitivity and measurable in real-time.
Asunto(s)
Pruebas Respiratorias/instrumentación , Espectrometría de Masas/instrumentación , Mentol/análisis , Aceites de Plantas/análisis , Compuestos Orgánicos Volátiles/análisis , Adulto , Presión Atmosférica , Diseño de Equipo , Espiración , Femenino , Humanos , Masculino , Mentha piperita , Mentol/metabolismo , Persona de Mediana Edad , Aceites de Plantas/administración & dosificación , Aceites de Plantas/metabolismo , Sistemas de Atención de Punto , Respiración , Compuestos Orgánicos Volátiles/metabolismo , Adulto JovenRESUMEN
High throughput screening identified a phenoxyacetic acid scaffold as a novel CRTh2 receptor antagonist chemotype, which could be optimised to furnish a compound with functional potency for inhibition of human eosinophil shape change and oral bioavailability in the rat.