RESUMEN
We report a spatial resolution of 5.4 nm in images of sarcoplasmic reticulum from rabbit muscle. The images were obtained in an aberration-corrected photoemission electron microscope with a hyperbolic mirror as the correcting element for spherical and chromatic aberration. In-situ measurements and numerical simulations confirm the low residual aberration in the instrument and indicate the ultimate resolution in this type of microscopy to be below 2 nm.
Asunto(s)
Microscopía Electrónica/métodos , Animales , Diseño de Equipo , Microscopía Electrónica/instrumentación , Conejos , Retículo Sarcoplasmático/ultraestructuraRESUMEN
A method based on matrix solid-phase dispersion (MSPD) is described for the quantitative extraction of glyphosate and its major metabolite aminomethylphosphonic acid (AMPA) from tomato fruit. After application of 120 microL of HNO3 1M to the sample, the dispersion column was packed with 0.5 g of sample blended into 1 g of NH2-silica. Two aqueous fractions were obtained. First, AMPA was eluted from the column using deionized water (F1), and then a NaH2PO4 0.005 M solution was used for the elution of glyphosate (F2). Cleanup of F1 and F2 was made by ion exchange chromatography on a SAX anion exchange silica. Determination was done by HPLC with fluorescence detection after precolumn derivatization with 9-fluorenylmethylchloroformate (FMOC-Cl). Mean recoveries calculated at fortification levels of 0.5 microg/g for glyphosate and 0.4 microg/g for AMPA were 87% and 78%, respectively. The relative standard deviations (n=7) for the total procedure were 10% and 16%. Detection limits were 0.05 microg/g for glyphosate and 0.03 microg/g for AMPA.
Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Glicina/análogos & derivados , Herbicidas/análisis , Organofosfonatos/análisis , Solanum lycopersicum/química , Espectrometría de Fluorescencia/métodos , Glicina/análisis , Isoxazoles , Reproducibilidad de los Resultados , Tetrazoles , GlifosatoAsunto(s)
2,4-Dinitrofenol/farmacología , Cuerpo Carotídeo/efectos de los fármacos , Cuerpo Carotídeo/metabolismo , Catecolaminas/metabolismo , Células Quimiorreceptoras/efectos de los fármacos , Células Quimiorreceptoras/fisiología , Animales , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio/efectos de los fármacos , Canales de Calcio/fisiología , Técnicas In Vitro , Potenciales de la Membrana/efectos de los fármacos , ConejosRESUMEN
In order to better understand the post-natal increase in peripheral chemoreceptor responsiveness to hypoxia, chemoreceptors of newborn (1-2 days) and older (10-12 days, 30 days, adult) rabbits were isolated and superfused, in vitro. The free tissue catecholamine concentration was measured using carbon-fiber voltammetry and pauci-fiber nerve activity was recorded from the sinus nerve during stimulation (4 min) with graded hypoxia or increased potassium. Both the peak catecholamine and peak nerve responses to stimulation with 10% and 0% oxygen increased with age, particularly between 10 and 30 days of age. In contrast, peak nerve and peak catecholamine responses to increased potassium did not significantly change with age. For a better understanding of how responsiveness increases with age, the fast Na+ and the Ca2+ currents were measured from isolated glomus cells of newborn and older rabbits, but the magnitude of the currents when normalized to membrane area was not significantly different between ages. We conclude that: (1) rabbit chemoreceptors mature in the newborn period (10-30 days) and part of this maturation is an increase in catecholamine secretion, (2) maturation of hypoxia transduction primarily occurs in steps prior to depolarization since potassium-evoked responses were not affected, and (3) an increase in the magnitude of glomus cell fast Na+ or Ca2+ currents is not a likely mechanism for the maturational change, but changes in the oxygen sensitivity of these currents cannot be excluded.
Asunto(s)
Calcio/metabolismo , Cuerpo Carotídeo/crecimiento & desarrollo , Células Quimiorreceptoras/crecimiento & desarrollo , Dopamina/metabolismo , Sodio/metabolismo , Factores de Edad , Animales , Canales de Calcio/fisiología , Cuerpo Carotídeo/química , Cuerpo Carotídeo/metabolismo , Células Quimiorreceptoras/metabolismo , Electrofisiología , Hipoxia/metabolismo , Hipoxia/fisiopatología , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Oxígeno/farmacología , Nervios Periféricos/efectos de los fármacos , Nervios Periféricos/fisiología , Potasio/farmacología , Conejos , Transducción de Señal/fisiología , Canales de Sodio/fisiología , Tetrodotoxina/farmacologíaRESUMEN
In an in vitro preparation of the intact carotid body (CB) of the rabbit, adenosine (100 microM) inhibited hypoxia-induced catecholamine release by 25%. The specific A1 antagonist, 8-cyclopentyl-1,3-dipropylxanthine (DPCPX; 1 microM) prevented the inhibition and increased the response to hypoxia further. In isolated chemoreceptor cells from the same species, adenosine inhibited voltage-dependent Ca2+ currents by 29% at 1 microM (concentration producing half-maximal inhibition, IC50 = 50 nM). This inhibition was mimicked by R(-)N6-(2-phenylisopropyl)-adenosine and 2-chloroadenosine (1 microM), two purinergic agonists poorly active at the intracellular ('P') site, and persisted in the presence of dipyridamole (a blocker of adenosine uptake; 1 microM) and was fully inhibited by 8-phenyltheophylline (10 microM). The A1 antagonists DPCPX (10 microM) and 8-cyclopentyl-1,3-dimethylxantine (0.1 microM) inhibited the effect of adenosine by 93% (IC50 = 0.14 microM) and 59%, respectively. The inhibition of the Ca2+ current (I(Ca)) was reduced by nisoldipine (an L-type Ca2+ channel antagonist) by nearly 50%, and was unaltered by omega-conotoxin GVIA, a blocker of N-type Ca2+ channels. Adenosine did not affect the voltage-dependent Na+ current (I(Na)) or K+ current (I(K)). We conclude that adenosine A1 receptors are located in chemoreceptor cells and mediate the inhibition of L-type Ca2+ channels and thereby the release of catecholamines produced by hypoxia. The data also indicate that endogenous adenosine acts as a physiological negative modulator of the chemoreceptor cell function. The previously reported excitatory action of adenosine on the activity of the sensory nerve of the CB is discussed in terms of a balance between the inhibition mediated by A1 receptors and the excitation mediated by A2 receptors.
Asunto(s)
Adenosina/farmacología , Canales de Calcio/fisiología , Cuerpo Carotídeo/química , Cuerpo Carotídeo/metabolismo , Catecolaminas/farmacocinética , Adenosina/análogos & derivados , Antagonistas Adrenérgicos alfa/farmacología , Animales , Calcio/farmacocinética , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio Tipo L , Cuerpo Carotídeo/efectos de los fármacos , Células Quimiorreceptoras/química , Células Quimiorreceptoras/metabolismo , Relación Dosis-Respuesta a Droga , Electrofisiología , Hipoxia/metabolismo , Activación del Canal Iónico/efectos de los fármacos , Activación del Canal Iónico/fisiología , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Fármacos Neuroprotectores/farmacología , Péptidos/farmacología , Potasio/farmacología , Conejos , Sodio/farmacología , Tetrodotoxina/farmacología , Teofilina/análogos & derivados , Teofilina/farmacología , Tritio , Xantinas/farmacología , omega-Conotoxina GVIARESUMEN
Current concepts on the location and functional significance of nicotinic receptors in the carotid body rest on alpha-bungarotoxin binding and autoradiographic studies. Using an in vitro preparation of the cat carotid body whose catecholamine deposits have been labeled by prior incubation with the tritiated natural precursor [3H]tyrosine, we have found that nicotine induces release of [3H]catecholamines in a dose-dependent manner (IC50 = 9.81 microM). We also found that mecamylamine (50 microM) completely abolished the nicotine-induced release, while alpha-bungarotoxin (100 nM; approximately 20 times its binding Kd) only reduced the release by 56%. These findings indicate that chemoreceptor cells, and perhaps other carotid body structures, contain nicotinic receptors that are not sensitive to alpha-bungarotoxin and force a revision of the current concepts on cholinergic mechanisms in the carotid body chemoreception.
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Cuerpo Carotídeo/fisiología , Catecolaminas/metabolismo , Células Quimiorreceptoras/fisiología , Mecamilamina/farmacología , Neuronas/fisiología , Nicotina/farmacología , Receptores Nicotínicos/fisiología , Ácido 3,4-Dihidroxifenilacético/metabolismo , Animales , Bungarotoxinas/metabolismo , Catecoles/metabolismo , Gatos , Técnicas In Vitro , Cinética , Neuronas/efectos de los fármacos , Receptores Nicotínicos/clasificación , Receptores Nicotínicos/efectos de los fármacos , Tritio , Tirosina/metabolismoRESUMEN
We have used the in vitro preparation of the intact carotid body (CB) and isolated chemoreceptor cells to elucidate the distribution and function of alpha2-adrenoreceptors. The significance of the study lies in the fact that norepinephrine (NE), being the neurotransmitter of the sympathetic innervation to the CB, is also abundant in chemoreceptor cells. In intact CB whose catecholamine (CA) deposits had been labeled by prior incubation with the CA precursor [3H]tyrosine, the alpha2-antagonist yohimbine (10 microM) potentiated the low-PO2 (33 and 60 mmHg)-induced release of [3H]CA by 100 and 53%, respectively. Yohimbine (10 microM) and SKF-86466 (50 microM; another alpha2-antagonist) reversed the inhibition of the release of [3H]CA produced by the alpha2-receptor agonists clonidine and UK-14304 (10 microM). The increase in adenosine 3',5'-cyclic monophosphate produced by low PO2 was further augmented by yohimbine and nearly halved by UK-14304 and clonidine. In isolated chemoreceptor cells, UK-14304 and NE inhibited voltage-dependent Ca2+ currents by 28 and 32%, respectively. These results indicate that alpha2-receptors are present in chemoreceptor cells, where they reduce the release of [3H]CA. Inhibition of adenylate cyclase(s) and Ca2+ channels may be involved in this effect. Using intact CB from normal and chronically sympathectomized animals, we demonstrated a specific accumulation of [3H]NE in intraglomic sympathetic endings. Hypoxia (PO2 approximately 33 mmHg) did not elicit release of [3H]NE from the sympathetic endings, but high extracellular K+ (K+(e)) induced a release of [3H]NE that was inhibited by alpha2-agonists and augmented by alpha2-antagonists. These findings demonstrate that alpha2-receptors are also present in the sympathetic endings of the CB, where they modulate the release of NE. As a whole, this work provides a more detailed understanding of the role of the sympathetic innervation in the control of the CB chemoreceptor function, including the cellular mechanisms of the action of NE.
Asunto(s)
Cuerpo Carotídeo/fisiología , Inhibición Neural/fisiología , Receptores Adrenérgicos alfa/fisiología , Animales , Cuerpo Carotídeo/citología , Células Quimiorreceptoras/citología , Células Quimiorreceptoras/metabolismo , Terminaciones Nerviosas/fisiología , Norepinefrina/fisiología , Conejos , Receptores Adrenérgicos alfa/metabolismo , Sistema Nervioso Simpático/fisiología , Distribución TisularRESUMEN
In the present article we review in a concise manner the literature on the mechanisms of O2 chemoreception in the carotid body of adult mammals. In the first section we describe the basic structure of the carotid body, and define this organ as a secondary sensory receptor. In the second section is presented the most relevant literature on the O2 metabolism in the carotid body to define the parameters of O2 chemoreception, including hypoxic thresholds and P50 of the hypoxic responses. The final section is devoted to the mechanisms of detection of the hypoxic stimulus. We provide the data in favor and against each of the current three models on O2 chemoreception: the membrane model, the metabolic hypothesis with its different versions and the NAD(P)H oxidase model.
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Cuerpo Carotídeo/fisiología , Oxígeno/sangre , Adenosina Trifosfato/fisiología , Adulto , Animales , Monóxido de Carbono/sangre , Cuerpo Carotídeo/efectos de los fármacos , Cuerpo Carotídeo/enzimología , Cuerpo Carotídeo/ultraestructura , Dopamina/metabolismo , Complejo IV de Transporte de Electrones/fisiología , Nervio Glosofaríngeo/fisiología , Homeostasis , Humanos , Peróxido de Hidrógeno/metabolismo , Hipoxia/sangre , Hipoxia/fisiopatología , Mamíferos/fisiología , Modelos Biológicos , NADH NADPH Oxidorreductasas/fisiología , NADPH Oxidasas , Proteínas del Tejido Nervioso/fisiología , Presión Parcial , Potasio/farmacología , Conejos , Transducción de Señal , Tetrodotoxina/farmacologíaAsunto(s)
Cuerpo Carotídeo/fisiología , Células Quimiorreceptoras/fisiología , Sensación/fisiología , Animales , Cuerpo Carotídeo/anatomía & histología , Cuerpo Carotídeo/metabolismo , Células Quimiorreceptoras/citología , Electrofisiología , Humanos , Neuronas Aferentes/fisiología , Sinapsis/fisiología , Transmisión SinápticaRESUMEN
Basal release of [3H]catecholamine ([3H]CA) from rabbit carotid bodies (CBs), previously incubated in the presence of [3H]tyrosine, was not significantly modified by prostaglandin E2 (PGE2). On the contrary, PGE2 (3-300 nM) produced a dose-dependent inhibition of the low PO2-evoked release of [3H]CA. The inhibition was greatest (55%) at a low intensity of hypoxic stimulation (incubating solution PO2 approximately 66 mmHg) and decreased with increasing intensities of hypoxia. Chronic denervation of the CB did not modify the response to PGE2. The release of [3H]CA induced by incubating the CBs in a hypercapnic-acidic solution (PCO2 approximately 132 mmHg; pH = 6.60) and by dinitrophenol (100 microM) was not significantly modified by 300 nM PGE2. PGE2 (300 nM) inhibited the release of [3H]CA elicited by incubating the CBs in a high K+ (35 mM)-containing solution. The release response elicited by high K+ (25 mM) was strongly augmented by a dihydropyridine agonist of Ca2+ channels, Bay K 8644, at a concentration of 1 microM. The Bay K 8644 effect was partly inhibited by PGE2 (300 nM). Using whole-cell recordings in freshly dispersed or short-term cultured chemoreceptor cells from adult rabbits it was found that Ca2+ currents (ICa) were reversibly inhibited by bath application of PGE2. A good parallelism exits between the dose-response curves for PGE2 inhibition of ICa in isolated chemoreceptor cells and high extracellular [K+]- or hypoxia-evoked release of [3H]CA from the whole CB.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio/metabolismo , Cuerpo Carotídeo/metabolismo , Catecolaminas/metabolismo , Dinoprostona/farmacología , Ácido 3-piridinacarboxílico, 1,4-dihidro-2,6-dimetil-5-nitro-4-(2-(trifluorometil)fenil)-, Éster Metílico/farmacología , Animales , Cuerpo Carotídeo/citología , Cuerpo Carotídeo/efectos de los fármacos , Desnervación , Electrofisiología , Proteínas de Unión al GTP/metabolismo , Técnicas In Vitro , Nisoldipino/farmacología , Conejos , Ganglio Cervical Superior/fisiologíaRESUMEN
The release of prostaglandin E2 (PGE2) from rabbit carotid bodies (CBs) incubated in basal conditions (PO2 approximately 132 mmHg; PCO2 approximately 33 mmHg; pH = 7.42) amounts to 94.4 +/- 10.1 pg (mg protein)-1 (10 min)-1 (mean +/- S.E.M.). Incubation of the CB in a hypoxic solution (PO2 approximately 46 mmHg) produced a significant 40% increase (P < 0.05) in the release of PGE2. Indomethacin (2 microM) prevented the hypoxia-induced release of PGE2. Sensory plus sympathetic denervation of the CB 4 days prior to the experiments did not modify either basal or low PO2-induced PGE2 release, indicating that intraglomic nerve endings are not significant sources for the PGE2 released. Incubation of the CB in an acidic-hypercapnic solution (PO2 approximately 132 mmHg; PCO2 approximately 132 mmHg; pH = 6.60) or in a high K(+)-containing solution (35 mM) was also effective in promoting an increase in the outflow of PGE2 from the organs. The release of [3H]catecholamines ([3H]CA) from the CB elicited by incubating the organs in low PO2 solutions (PO2 ranged between 66 and 13 mmHg) was potentiated by two inhibitors of cyclo-oxygenase, acetylsalicylic acid (ASA, 100 microM) and indomethacin (2 microM). The effect persisted after chronic denervation of the organ. The secretory response elicited by acidic stimuli was also augmented by cyclo-oxygenase inhibitors. Thus, [3H]CA release elicited by incubating the CBs in the acidic-hypercapnic solution increased by 300% in the presence of indomethacin (2 microM), and ASA (100 microM) more than doubled the release induced by dinitrophenol (100 microM), a protonophore that mimics an acidic stimulus. Indomethacin, but not ASA, moderately increased the high K(+)-evoked [3H]CA release. The effect of indomethacin on the release of [3H]CA elicited by acidic and hypoxic stimuli was reversed by PGE2 in a dose-dependent manner (0.3-300 nM). These results show that low PO2 and high PCO2-low pH, the natural stimuli to the CB, as well as high extracellular [K+], activate the cyclo-oxygenase pathway in the CB, promoting an increase in the outflow of PGE2. The data also show that the blockade of this pathway activates the stimulus-induced [3H]CA release from the CB, indicating that naturally released prostanoids exert an inhibitory control on chemoreceptor cells. The data lend support to the notion that the hyper-reactivity of the ventilatory response to hypoxia in subjects under anti-inflammatory drug treatment results from CB cycloxygenase inhibition.
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Cuerpo Carotídeo/enzimología , Catecolaminas/metabolismo , Inhibidores de la Ciclooxigenasa/farmacología , Prostaglandina-Endoperóxido Sintasas/metabolismo , Animales , Aspirina/farmacología , Dióxido de Carbono/farmacología , Cuerpo Carotídeo/efectos de los fármacos , Cuerpo Carotídeo/metabolismo , Dinoprostona/metabolismo , Activación Enzimática/efectos de los fármacos , Concentración de Iones de Hidrógeno , Técnicas In Vitro , Indometacina/farmacología , ConejosAsunto(s)
Cuerpo Carotídeo/metabolismo , Catecolaminas/metabolismo , AMP Cíclico/metabolismo , Neurotransmisores/metabolismo , Sistemas de Mensajero Secundario , 1-Metil-3-Isobutilxantina/farmacología , Animales , Betanecol , Compuestos de Betanecol/farmacología , Cuerpo Carotídeo/efectos de los fármacos , Dinoprostona/farmacología , Dopamina/farmacología , Femenino , Técnicas In Vitro , Isoproterenol/farmacología , Cinética , Masculino , Conejos , Sistemas de Mensajero Secundario/efectos de los fármacos , Serotonina/farmacología , Sustancia P/farmacología , Tirosina/metabolismoRESUMEN
This study presents evidence that dopaminergic neurons innervate the cat carotid body. Immunocytochemical studies revealed many tyrosine hydroxylase (TH)-positive nerve fibers in the carotid body which establish extensive contacts with type I cells. All TH-positive intralobular profiles disappeared with chronic carotid sinus nerve (CSN) section, but survived sympathectomy following removal of the superior cervical ganglion. The level of endogenous dopamine (DA) in the CSN was higher than that for norepinephrine (NE). While both catecholamines were synthesized by the nerve at similar rates, NE synthesis was abolished by chronic sympathectomy, but DA synthesis remained largely unchanged following this procedure. Our data indicate that DA is not present in the CSN as a mere precursor of NE. Following a 3-hour incubation of carotid bodies with their attached nerves in media containing 20 microM 3H-tyrosine, electrical stimulation of CSN C-fibers in chronically sympathectomized preparations provoked the release of 3H-DA, but not 3H-NE.
Asunto(s)
Cuerpo Carotídeo/metabolismo , Seno Carotídeo/metabolismo , Dopamina/metabolismo , Animales , Cuerpo Carotídeo/citología , Gatos , Dopamina/biosíntesis , Estimulación Eléctrica , Nervio Glosofaríngeo/metabolismo , Inmunohistoquímica , Técnicas In Vitro , Fibras Nerviosas/metabolismo , Norepinefrina/biosíntesis , Norepinefrina/metabolismo , Simpatectomía , Tirosina 3-Monooxigenasa/metabolismoRESUMEN
1. Almitrine increases ventilation by stimulating the carotid body (CB) arterial chemoreceptors but neither its intraglomic target nor its mechanism of action have been elucidated. 2. We have tested the hypothesis that chemoreceptor cells are targets for almitrine by studying its effects on the release of 3H-catecholamines in an in vitro rabbit CB preparation. 3. It was found that almitrine (0.3 and 1.5 x 10(-6) M; i.e. 0.2 and 1 mg ml-1) increases the resting release of 3H-catecholamines from CBs (previously loaded with [3H]-tyrosine) incubated in a balanced 95% O2/5% CO2-equilibrated solution. 4. Almitrine at a concentration of 3 x 10(-6) M (2 mg l-1) also augmented the release of 3H-catecholamines elicited by incubating the CBs in a hypoxic solution (equilibrated with 7% O2/5% CO2 in N2), by high external K+ (35 mM) and by veratridine (2 x 10(-5) M), but did not modify release induced by dinitrophenol (7.5 x 10(-5) M). 5. At the same concentration (3 x 10(-6) M), almitrine increased the rate of dopamine synthesis and was ineffective in modifying the cyclic AMP levels in either normoxic or hypoxic CBs. 6. It is concluded that chemoreceptor cells are the intraglomic targets for almitrine. The mechanisms of action of almitrine on chemoreceptor cells are discussed.
Asunto(s)
Almitrina/farmacología , Cuerpo Carotídeo/metabolismo , Catecolaminas/metabolismo , Animales , Catecolaminas/biosíntesis , Técnicas In Vitro , Malatos/farmacología , Conejos , TritioRESUMEN
Salicylates, at the high therapeutic doses used in the treatment of rheumatoid arthritis, produce an increase in ventilation and augment the carotid body reactivity to hypoxic stimulus, leading to an exaggerated hyperventilation during hypoxia. These effects had been related to the action of salicylates as uncouplers of oxidative phosphorylation. In the present study, carried out in an in vitro preparation of the rabbit carotid body, we show that acetylsalicylic acid and indomethacin, two anti-inflammatory drugs that are also powerful inhibitors of cyclooxygenase, the prostaglandin-synthetizing enzyme, produce an increase in the [3H]catecholamine release evoked by low oxygen stimulation. The drugs did not affect basal normoxic release, a finding that suggests that at the concentration used these anti-inflammatory agents do not have uncoupling actions, and that their effects on hypoxic-induced release of [3H]catecholamines is mediated by their specific action as cyclooxygenase inhibitors. In agreement with this suggestion we found that prostaglandin E2 completely prevented the effects of both anti-inflammatory agents. In addition, our data indicate that endogenously synthetized prostaglandins are powerful modulators of chemoreceptor cell function.
Asunto(s)
Cuerpo Carotídeo/fisiología , Catecolaminas/metabolismo , Inhibidores de la Ciclooxigenasa/farmacología , Dinoprostona/farmacología , Animales , Aspirina/farmacología , Cuerpo Carotídeo/efectos de los fármacos , Técnicas In Vitro , Indometacina/farmacología , Cinética , ConejosRESUMEN
The carotid bodies are arterial chemoreceptors that are sensitive to blood PO2, PCO2 and pH. They are the origin of reflexes that are crucial for maintaining PCO2 and pH in the internal milieu and for adjusting the O2 supply according to the metabolic needs of the organism in situations of increased demand, such as exercise and while breathing at decreased O2 partial pressures during ascent or when living at high altitude. Chemoreceptor cells of the carotid body transduce the blood-borne stimuli into a neurosecretory response that is dependent on external Ca2+. These cells have an O2-sensitive K+ current that is reversibly inhibited by low PO2. It is proposed that the depolarization produced by inhibition of this K+ current activates Ca2+ channels; Ca2+ influx and neurosecretion follow. The cells have also a potent Na(+)-Ca2+ antiporter that could be responsible for the intracellular Ca2+ rise required to trigger the release of neurotransmitters during high PCO2 or low pH stimulation.
Asunto(s)
Ácidos/farmacología , Cuerpo Carotídeo/fisiología , Células Quimiorreceptoras/fisiología , Oxígeno/farmacología , Animales , Cuerpo Carotídeo/efectos de los fármacos , Células Quimiorreceptoras/efectos de los fármacos , HumanosRESUMEN
We have investigated the effects of different treatments that increase cyclic AMP levels on the in vitro synthesis and release of catecholamines in the rabbit carotid body. We also measured the rate of 45Ca2+ efflux from previously loaded carotid bodies under different conditions. Forskolin produced a dose-dependent increase in the release of [3H]dopamine elicited by a hypoxic stimulus of medium intensity (PO2 = 33 mm Hg) without altering basal [3H]dopamine release (100% O2-equilibrated medium). At a concentration of 5 x 10(-6) M, forskolin increased the release of [3H]dopamine induced by hypoxic stimuli of different intensities; the increase was maximal (498%) at the lowest intensity of hypoxic stimuli (PO2 = 66 mm Hg), averaged 260% for hypoxic stimuli of intermediate intensity and 2 x 10(-4) M cyanide, and was 150% under anoxia. Dibutyryl cyclic AMP (2 mM) and 3-isobutyl-1-methylxanthine (0.5 mM) mimicked forskolin effects under hypoxic stimulation. Forskolin (5 x 10(-6) M) also increased (180%) the release of [3H]dopamine induced by 20% CO2/pH 6.6, 2.5 x 10(-4) M dinitrophenol, and 3 x 10(-5) M ionomycin. Forskolin and 3-isobutyl-1-methylxanthine were without effect on the release of [3H]dopamine elicited by 30 mM extracellular K+. Forskolin (5 x 10(-6) M) augmented significantly the rate of 45Ca2+ efflux induced by hypoxic stimuli (PO2 of 33 and 66 mm Hg) and 2 x 10(-4) M cyanide and showed a tendency to increase (20%) the 45Ca2+ efflux induced by dinitrophenol and low pH and to decrease (21%) the efflux induced by 30 mM K+ without altering the rate of efflux under basal conditions.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Cuerpo Carotídeo/metabolismo , AMP Cíclico/fisiología , Dopamina/metabolismo , Hipoxia/metabolismo , 1-Metil-3-Isobutilxantina/farmacología , Animales , Bucladesina/farmacología , Calcio/metabolismo , Colforsina/farmacología , Dopamina/biosíntesis , Hipoxia/fisiopatología , Norepinefrina/biosíntesis , Oxígeno , Presión Parcial , Conejos , Estimulación QuímicaRESUMEN
Rabbit carotid bodies incubated in vitro in the presence of 5 x 10(-4) M 3-isobutyl 1-methylxanthine have cyclic AMP levels of 13.1 +/- 1.6 pmol/mg fresh tissue (means +/- S.E.M; n = 11). Dopamine (10(-6) and 10(-5) M) increased the cyclic AMP content to 24.4 +/- 2.85 and 40.6 +/- 3.29 pmol/mg tissue. The specific D1 agonist SKF38393 at 10(-6) M increased the cyclic AMP content to 23.9 +/- 2.3 pmol/mg fresh tissue and the specific D1 antagonist SCH23390 at 10(-6) M completely blocked the effect of 10(-5) M dopamine. dBcAMP (2 x 10(-3) M) potentiated the release of dopamine induced by a mild hypoxic stimulus and SKF38393 did not modify it. It is concluded that the carotid body has D1 receptors positively coupled to adenylate cyclase that seem to be located in the vasculature of the organ. This set of receptors, when activated by injected dopamine, produced vasodilatation leading to a decrease in carotid sinus nerve activity.
Asunto(s)
Cuerpo Carotídeo/metabolismo , Receptores Dopaminérgicos/metabolismo , 2,3,4,5-Tetrahidro-7,8-dihidroxi-1-fenil-1H-3-benzazepina/farmacología , Adenilil Ciclasas/metabolismo , Animales , Vasos Sanguíneos/metabolismo , Bucladesina/farmacología , Cuerpo Carotídeo/irrigación sanguínea , AMP Cíclico/metabolismo , Dopaminérgicos/farmacología , Hipoxia/metabolismo , Conejos , Receptores de Dopamina D1RESUMEN
Acetylcholine and muscarinic agonists inhibit chemosensory activity in the rabbit carotid sinus nerve (CSN). Because the mechanism of this inhibition is poorly understood, we have investigated the kinetics and distribution of muscarinic receptors in the rabbit carotid body with the specific muscarinic antagonist [3H]quinuclidinylbenzilate ([3H]QNB). Equilibrium binding experiments identified displaceable binding sites (1 microM atropine) with a Kd = 71.46 pM and a Bmax = 9.23 pmol/g tissue. These binding parameters and the pharmacology of the displaceable [3H]QNB binding sites are similar to specific muscarinic receptors identified in numerous other nervous, muscular and glandular tissues. Comparisons of specific binding in normal and chronic CSN-denervated carotid bodies suggest that muscarinic receptors are absent on afferent terminals in the carotid body; however, nearly 50% of the specific [3H]QNB binding is lost following chronic sympathectomy, suggesting the presence of presynaptic muscarinic receptors on the sympathetic innervation supplying the carotid body vasculature. Autoradiographic studies have localized the remainder of [3H]QNB binding sites to lobules of type I and type II parenchymal cells. In separate experiments, the muscarinic agonists, oxotremorine (100 microM) stimulation of the in vitro carotid body. Our data suggest that muscarinic inhibition in the rabbit carotid body is mediated by receptors located on type I cells which are able to modulate the excitatory actions of acetylcholine at nicotinic sites.
Asunto(s)
Cuerpo Carotídeo/química , Receptores Muscarínicos/análisis , Animales , Autorradiografía , Seno Carotídeo/inervación , Catecolaminas/metabolismo , Femenino , Cinética , Masculino , Conejos , Ensayo de Unión Radioligante , Receptores Muscarínicos/fisiologíaRESUMEN
Cyclic AMP levels in rabbit carotid bodies incubated under control conditions, 100% O2- or 95% O2/5% CO2- equilibrated medium, are close to 1 pmol/mg wet tissue (range 0.4-2.43 pmol/mg). Isobutylmethylxanthine (0.5 mM) increases cyclic AMP levels by a factor of 14 and 8 in HEPES- and CO2/CH3O(-)-buffered medium, respectively. Forskolin (0.5-10 microM) applied during 30 min increases cyclic AMP levels in a dose-dependent manner. Incubation of carotid bodies at low O2 tensions resulted in an elevation of cyclic AMP levels both in the absence and in the presence of isobutymethylxanthine. In the latter conditions cyclic AMP increase was maximum at an O2 tension of 46 mm Hg and tended to decrease at extremely low PO2. In isobutylmethylxanthine-containing Ca2(+)-free medium, cyclic AMP increased linearly with decreasing PO2 from 66 to 13 mm Hg; the absolute cyclic AMP levels attained in Ca2(+)-free medium were smaller than those observed in Ca2(+)-containing medium at any PO2. The differences between Ca2(+)-free and Ca2(+)-containing media appear to be due to the action of released neurotransmitters in the latter conditions, because dopamine and norepinephrine, which are known to be released by hypoxia in a Ca2(+)-dependent manner, increase cyclic AMP in the carotid body. Low pH/high PCO2 and high [K+]e increase cyclic AMP levels only in Ca2(+)-containing medium. Forskolin potentiates the release of catecholamines induced by low PO2. These results suggest that cyclic AMP plays an important role in the modulation of the chemoreception process.