RESUMEN
Kuru is a prion disease which became epidemic among the Fore and surrounding linguistic groups in Papua New Guinea, peaking in the late 1950s. It was transmitted during the transumption (endocannibalism) of dead family members at mortuary feasts. In this study, we aimed to explain the historical spread and the changing epidemiological patterns of kuru by analysing factors that affected its transmission. We also examined what cultural group principally determined a family's behaviour during mortuary rituals. Our investigations showed that differences in mortuary practices were responsible for the initial pattern of the spread of kuru and the ultimate shape of the epidemic, and for subsequent spatio-temporal differences in the epidemiology of kuru. Before transumption stopped altogether, the South Fore continued to eat the bodies of those who had died of kuru, whereas other linguistic groups, sooner or later, stopped doing so. The linguistic group was the primary cultural group that determined behaviour but at linguistic boundaries the neighbouring group's cultural practices were often adopted. The epidemiological changes were not explained by genetic differences, but genetic studies led to an understanding of genetic susceptibility to kuru and the selection pressure imposed by kuru, and provided new insights into human history and evolution.
RESUMEN
The recognition of the first cases of variant Creutzfeldt-Jakob Disease in the United Kingdom (UK) in 1996 and the realisation that this new disease represented the human form of the cattle disease BSE has prompted a considerable investment in research, particularly in the UK, Europe and the United States (US). Much has been learnt about this disease but much is still unknown. Infectivity is not destroyed by conventional sterilisation and disinfection treatment methods. This, combined with the widespread distribution throughout the lymphoid system as well as the central nervous system, raises the spectre of transmission through both surgical and ophthalmological procedures. Reports in 2004 of two likely transfusion-transmitted cases of vCJD suggest the probability of infection through blood transfusion and tissue transplantation. The risk of hospital-based and community-based transmission has not been quantified. To complicate matters even further, there is no suitable ante-mortem screening test or effective treatment for this fatal disease. The incubation period prior to onset of clinical disease is many years and there is good evidence for the existence of subclinical infection and infectivity during this stage. The extent of under-diagnosis and misdiagnosis is probably significant, adding to the risk of human-to-human transmission.
Asunto(s)
Síndrome de Creutzfeldt-Jakob , Atención a la Salud , Animales , Australia/epidemiología , Bovinos , Síndrome de Creutzfeldt-Jakob/epidemiología , Síndrome de Creutzfeldt-Jakob/prevención & control , Síndrome de Creutzfeldt-Jakob/transmisión , Encefalopatía Espongiforme Bovina/diagnóstico , Encefalopatía Espongiforme Bovina/epidemiología , Humanos , Factores de Riesgo , Procedimientos Quirúrgicos Operativos/efectos adversos , Reacción a la Transfusión , Reino Unido/epidemiología , Estados UnidosRESUMEN
The aims of this descriptive study were to confirm the high incidence of subacute sclerosing panencephalitis (SSPE) previously reported from Papua New Guinea (PNG) and to relate SSPE to previous measles vaccination and measles illness. From February 1997 to April 1999 we diagnosed a total of 55 patients with SSPE at Goroka Base General Hospital in Eastern Highlands Province (EHP) of PNG. The diagnosis was based on high cerebrospinal fluid and serum measles virus antibody titres with progressive neurological disorder and myoclonic jerks. Of these 55 patients 42 were from EHP, including 32 whose onset was in the 2-year period 1997-1998. The annual incidence of SSPE in EHP in these 2 years was 98 per million population under 20 years of age, the highest ever reported. This incidence was more than ten times higher than the highest incidence in the prevaccine era reported from elsewhere. The mean age of onset of SSPE was 7.7 years (range 2.8-14.8 years) and the interval between measles and the onset of SSPE, where known, had a mean of 5.9 years and a range of 2.5-11.1 years. Among the SSPE patients 19 had a documented history of measles vaccination. Eight of these 19 also had documentation of previous measles illness; of these, seven were vaccinated after the development of measles and one was vaccinated 20 days before measles illness. Two non-SSPE children received vaccination twice which was documented and subsequently developed measles which was also substantiated by documentation. Two patients with SSPE yielded amplified nucleotide sequences of measles virus that were different from any of the vaccine strains. We found no evidence to implicate measles vaccination in the development of SSPE.
Asunto(s)
Panencefalitis Esclerosante Subaguda/epidemiología , Enfermedad Aguda , Adolescente , Niño , Preescolar , Femenino , Humanos , Inmunoglobulina G/análisis , Incidencia , Lactante , Masculino , Sarampión/complicaciones , Vacuna Antisarampión/efectos adversos , Papúa Nueva Guinea/epidemiología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Panencefalitis Esclerosante Subaguda/etiologíaRESUMEN
Nine-year follow-up (ending 1999) of survival of 3738 individuals in a malaria-endemic area of Papua New Guinea found that the use of mosquito nets was associated with a large reduction in mortality in people aged > or = 40 years as well as in children aged < 5 years. There may be substantial benefits of malaria transmission reduction for older people, that have been overlooked in public health programmes and burden of disease calculations.
Asunto(s)
Malaria Falciparum/prevención & control , Control de Mosquitos/métodos , Adolescente , Adulto , Anciano , Niño , Preescolar , Estudios de Seguimiento , Humanos , Lactante , Recién Nacido , Malaria Falciparum/epidemiología , Persona de Mediana Edad , Papúa Nueva Guinea/epidemiología , Prevalencia , Análisis de Regresión , Análisis de SupervivenciaRESUMEN
The relationship between filarial antigenemia and lymphatic pathology was investigated in residents of 11 villages in an area of Papua New Guinea where Wuchereria bancrofti is endemic. Antigenemia was determined in 1322 persons by means of the Og4C3 antibody capture assay. Prevalence of antigenemia by village ranged from 61.7% to 98.2% and did not vary by sex. Antigen level increased with transmission potential among the 4 villages with measured transmission potential (r(2)=.945; P=.028). Antigenemia was associated positively with age in villages with the lowest annual transmission potentials (45 and 404 infective larvae/year; P<.001), but was distributed evenly across age groups in villages with increased transmission (1485 and 2518 infective larvae/year). These data suggest that children and adults have similar worm burdens in areas of high transmission, whereas worm burdens in areas of lower transmission increase with age. These results may be useful in the design and evaluation of programs aimed at eliminating lymphatic filariasis.
Asunto(s)
Antígenos Helmínticos/sangre , Filariasis/epidemiología , Filariasis/inmunología , Linfedema/inmunología , Wuchereria bancrofti/inmunología , Adolescente , Adulto , Anciano , Animales , Niño , Preescolar , Femenino , Filariasis/transmisión , Humanos , Lactante , Recién Nacido , Linfedema/patología , Masculino , Persona de Mediana Edad , Papúa Nueva Guinea/epidemiología , Parasitemia , Prevalencia , Wuchereria bancrofti/crecimiento & desarrolloAsunto(s)
Dietilcarbamazina/uso terapéutico , Filariasis Linfática/prevención & control , Filaricidas/uso terapéutico , Ivermectina/uso terapéutico , Adulto , Filariasis Linfática/epidemiología , Emigración e Inmigración , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Masculino , Papúa Nueva Guinea/epidemiología , Prevalencia , Salud Rural , Salud UrbanaRESUMEN
In an area of Papua New Guinea with high prevalence of Plasmodium falciparum (39.6%), Plasmodium vivax (18.3%), and Plasmodium malariae (13.8%), cross-sectional analysis found P. falciparum infection to be independent of the other species despite heterogeneities in transmission. Plasmodium vivax and P. malariae infections were negatively correlated. Plasmodium malariae infection was positively associated with homologous infection four months previously and with prior P. falciparum, but not P. vivax infection. There were no other indications that any Plasmodium species protected against heterologous infection. Prospective analysis of health-center morbidity supported the idea that P. malariae infection protects against disease, but indicated greater protection against non-malaria than P. falciparum-associated fevers. Plasmodium vivax appeared to protect against P. falciparum disease but not against other forms of morbidity. Covariate adjustment had considerable effects on estimated relationships between species, and confounding variables may account for many differences among reports of inter-species interactions in human malaria.
Asunto(s)
Malaria/fisiopatología , Plasmodium/aislamiento & purificación , Animales , Estudios Transversales , Humanos , Malaria/epidemiología , Malaria/parasitología , Morbilidad , Nueva Guinea/epidemiología , Plasmodium/clasificación , Estudios Prospectivos , Factores de Riesgo , Especificidad de la EspecieRESUMEN
A point mutation in the promoter of the nitric oxide synthase 2 gene (NOS2), termed NOS2(Lambaréné) (NOS2-G954C), protects heterozygous carriers against severe malaria as effectively as the sickle cell trait. In a prospective longitudinal study, 841 individual infections of initially 200 children (151 wild-type vs. 49 NOS2(Lambaréné) carriers) were monitored for 4 years, to assess the rates of malarial attacks in the 2 groups; carriers of the NOS2(Lambaréné) polymorphism were significantly less likely to experience malarial attacks than were others (P=.002). The distribution of the NOS2(Lambaréné) polymorphism was investigated in malaria-endemic areas. It was found to be present with the highest frequency in Africa and at a lower frequency in Asia. Ex vivo studies showed that cells isolated from people with this polymorphism have a 7-fold higher baseline NOS activity, compared with the levels detected in cells from subjects with the wild-type gene (P=.003).
Asunto(s)
Frecuencia de los Genes , Inmunidad Innata/genética , Malaria/genética , Óxido Nítrico Sintasa/genética , Óxido Nítrico Sintasa/metabolismo , Óxido Nítrico/biosíntesis , Mutación Puntual , Polimorfismo de Nucleótido Simple , Estudios de Casos y Controles , Niño , ADN/sangre , Femenino , Gabón , Tamización de Portadores Genéticos , Alemania , Humanos , Malaria/fisiopatología , Masculino , Nigeria , Óxido Nítrico Sintasa de Tipo II , Papúa Nueva Guinea , Reacción en Cadena de la Polimerasa , Senegal , TailandiaRESUMEN
Humans living in areas where filariasis is endemic vary greatly in their exposure to mosquito-borne infective third-stage larvae (L3) of these parasitic helminths. Because the intensity of exposure to Ags affects T cell differentiation and susceptibility to parasitic infections in murine models, we compared T cell and cytokine responses in 97 residents of two villages in Papua New Guinea, where transmission intensity of Wuchereria bancrofti differed by 63-fold (37 vs 2355 L3 per person per year). Residents of the high transmission village had 4- to 11-fold lower proliferation and IFN-gamma responses to filarial Ags, nonparasite Ag, and PHA by PBMC compared with the low transmission village (p < 0.01) even when subjects were matched for intensity of infection. In contrast, filarial Ag-driven IL-5 production was 5.5-fold greater (p < 0.001), and plasma IL-4 and TGF-beta levels were 4-fold and 34% higher, respectively, in residents of the high transmission village. IL-4 and IL-10 responses by PBMC differed little according to village, and increased production of the counterregulatory cytokines IL-10 or TGF-beta by PBMC did not correlate with weak proliferation and IFN-gamma responses. Plasma IL-5, IFN-gamma, and IL-10 levels were similar in the two villages. These data demonstrate that the intensity of exposure to L3 affects lymphocyte responsiveness and cytokine bias possibly by a mechanism that alters APC function.
Asunto(s)
Citocinas/biosíntesis , Filariasis Linfática/inmunología , Filariasis Linfática/transmisión , Linfocitos T/inmunología , Linfocitos T/metabolismo , Adolescente , Adulto , Animales , Niño , Citocinas/antagonistas & inhibidores , Filariasis Linfática/epidemiología , Filariasis Linfática/parasitología , Femenino , Humanos , Tolerancia Inmunológica/efectos de los fármacos , Interleucina-4/sangre , Ionomicina/farmacología , Activación de Linfocitos/efectos de los fármacos , Masculino , Persona de Mediana Edad , Papúa Nueva Guinea/epidemiología , Fitohemaglutininas/farmacología , Índice de Severidad de la Enfermedad , Acetato de Tetradecanoilforbol/farmacología , Células Th2/inmunología , Células Th2/metabolismo , Wuchereria bancrofti/inmunologíaRESUMEN
Effects of bednet coverage (C) on prevalence of malaria were analysed using data from 1990-92 from 9 Papua New Guinean villages. Effects of coverage varied by age, resulting in a shift in age of peak prevalence from 4.7 (C = 0%) to 11.6 (C = 100%) years for Plasmodium falciparum, from 3.4 to 4.9 years for P. vivax and from 11.0 to 16.8 years for P. malariae. In small areas with no bednets the age distribution of P. falciparum parasitaemia was like that of a holoendemic area. Where coverage was complete the pattern corresponded to mesoendemicity. Thus, protracted use of bednets can result in profound changes in the endemicity of malaria even when coverage is incomplete and without insecticide treatment. Average entomological inoculation rates (EIRs) estimated from indoor landing rates on individuals without bednets were 35, 12 and 10 infectious bites per person per annum for P. falciparum, P. vivax and P. malariae, respectively. Logistic regression analyses indicated that the EIR estimate for P. falciparum was related to prevalence of this species independently of effects of bednet coverage. However, the recent EIR still accounted for much less variation than did the bednets. A similar pattern was seen for P. malariae, while there were no significant relationships between the recent EIR and the parasite positivity for P. vivax. It is concluded that short-term variations in inoculation rate are not important determinants of parasite prevalence in this population.
Asunto(s)
Ropa de Cama y Ropa Blanca , Malaria/prevención & control , Adolescente , Distribución por Edad , Animales , Niño , Preescolar , Ensayo de Inmunoadsorción Enzimática , Humanos , Modelos Logísticos , Malaria/epidemiología , Malaria Falciparum/epidemiología , Malaria Falciparum/prevención & control , Malaria Vivax/epidemiología , Malaria Vivax/prevención & control , Control de Mosquitos/métodos , Papúa Nueva Guinea/epidemiología , Plasmodium falciparum , Plasmodium malariae , Plasmodium vivax , PrevalenciaRESUMEN
Relationships between area coverage with insecticide-free bednets and prevalence of Plasmodium falciparum were investigated in 7 community-based surveys over a 33-month period in 1990-93 in 6 villages in the Wosera area of Papua New Guinea. Spatial patterns in circumsporozoite rates for P. falciparum, P. vivax isomorphs K210 and K247, and P. malariae, and the proportions of mosquito blood meals positive for specific human, goat, cat, dog and pig antigens were determined using ELISAs. P. falciparum prevalence in humans was better explained by bednet coverage in the immediate vicinity than by personal protection alone. Circumsporozoite rates for both P. falciparum and P. vivax were also inversely related to coverage with bednets. There was some increase in zoophagy in areas with high coverage, but relatively little effect on the human blood index or on overall mosquito densities. In this setting, protracted use of untreated bednets apparently reduces sporozoite rates, and the associated effects on prevalence are greater than can be accounted for by personal protection. Even at high bednet coverage most anophelines feed on human hosts, so the decreased sporozoite rates are likely to be largely due to reduction of mosquito survival. This finding highlights the importance of local vector ecology for outcomes of bednet programmes and suggests that area effects of untreated bednets should be reassessed in other settings.
Asunto(s)
Ropa de Cama y Ropa Blanca , Malaria Falciparum/prevención & control , Control de Mosquitos/métodos , Adolescente , Adulto , Distribución por Edad , Animales , Anopheles/parasitología , Apicomplexa , Niño , Preescolar , Ensayo de Inmunoadsorción Enzimática , Humanos , Lactante , Recién Nacido , Insectos Vectores , Modelos Logísticos , Malaria Falciparum/epidemiología , Papúa Nueva Guinea/epidemiología , PrevalenciaAsunto(s)
Enfermedades Endémicas/estadística & datos numéricos , Malaria/diagnóstico , Malaria/epidemiología , Hemorragia Posparto/diagnóstico , Hemorragia Posparto/epidemiología , Adolescente , Adulto , Comorbilidad , Países en Desarrollo , Femenino , Humanos , Incidencia , Papúa Nueva Guinea/epidemiología , Embarazo , Sistema de Registros , Medición de RiesgoRESUMEN
Kuru reached epidemic proportions by the mid-twentieth century among the Fore people of New Guinea and disappeared after the abolition of cannibalistic rituals. To determine susceptibility to kuru and its role in the spread and elimination of the epidemic, we analyzed the PRNP gene coding sequences in 5 kuru patients; no germline mutations were found. Analysis of the PRNP 129 methionine (M)/valine (V) polymorphism in 80 patients and 95 unaffected controls demonstrated that the kuru epidemic preferentially affected individuals with the M/M genotype. A higher representation of M/M carriers was observed among the affected young Fore males entering the age of risk, whereas a lower frequency of M/M homozygotes was found among the survivors. M/V and V/V genotypes predisposed to a lower risk of disease development and longer incubation times. These findings are relevant to the current outbreak of variant Creutzfeldt-Jakob disease (vCJD) in the United Kingdom, because all vCJD patients tested thus far have been M/M carriers.
Asunto(s)
Amiloide/genética , Brotes de Enfermedades , Predisposición Genética a la Enfermedad , Kuru/genética , Metionina/genética , Precursores de Proteínas/genética , Niño , Preescolar , Codón , Femenino , Genotipo , Humanos , Lactante , Kuru/epidemiología , Masculino , Mutación , Nueva Guinea/epidemiología , Polimorfismo Genético , Proteínas Priónicas , Priones , Análisis de Secuencia de ADNRESUMEN
BACKGROUND: In view of high mortality and morbidity from Haemophilus influenzae type b (Hib) in young Papua New Guinean children, the incorporation of a Hib conjugate vaccine into a nationwide immunization program would be of major public health benefit. METHODS: We evaluated the safety and immunogenicity of a lyophilized and a liquid form of Hib polysaccharide-tetanus toxoid conjugate vaccines (PRP-T) given in the same syringe as diphtheria-tetanus-pertussis (DTP) vaccine to children in Goroka, Eastern Highlands Province. In Part 1 of the study 209 children were randomized to receive at ages 1, 2 and 3 months either DTP alone or a liquid formulation of DTP/PRP-T or lyophilized PRP-T dissolved in DTP suspension. A further 75 children were given the liquid DTP/PRP-T formulation at ages 2, 3 and 4 months (Part 2). 54 children aged 15-18 months were given a booster of the same preparation of PRP-T/DTP as they had received during Part 1. Blood for antibody assays was collected at enrolment, before (Part 1 only) and one month after the third dose, then just before and 3 weeks after the booster dose. RESULTS: Follow-up to age of 12 months showed that PRP-T was safe with no evidence of impaired response to individual vaccine components when combined with DTP. Geometric mean titres (GMTs) of anti-PRP antibody before vaccination (n = 64, mean age 41 days), after 2 doses (mean age 99 days) and after 3 doses (mean age 132 days) of the lyophilized formulation were 0.21, 1.48 and 5.04 microg/ml, respectively, with 58% and 89% having anti-PRP antibody titres > or = 1.0 microg/ml after 2 and 3 doses, respectively. Anti-PRP antibody responses to the liquid Hib vaccine formulation were lower (GMT post-dose 3 = 0.48 microg/ml) than to the lyophilized formulation, but better responses were elicited from older children (Part 2; GMT post-dose 3 = 0.78 microg/ml, with 79% > or = 0.15 microg/ml). Both PRP-T preparations elicited excellent booster responses suggesting that children are likely to be protected if exposed to Hib infection. CONCLUSIONS: Lyophilized PRP-T given together with DTP is safe and immunogenic when given to young infants. The liquid DTP/PRP-T formulation showed a lower immunogenicity than in earlier studies with this vaccine, which might have been due to exposure to low temperature during shipment or the younger age at immunization.
Asunto(s)
Vacuna contra Difteria, Tétanos y Tos Ferina/administración & dosificación , Infecciones por Haemophilus/inmunología , Infecciones por Haemophilus/prevención & control , Vacunas contra Haemophilus/administración & dosificación , Esquemas de Inmunización , Toxoide Tetánico/administración & dosificación , Vacunación/métodos , Administración Oral , Análisis de Varianza , Distribución de Chi-Cuadrado , Preescolar , Relación Dosis-Respuesta a Droga , Femenino , Humanos , Inmunidad/fisiología , Inmunización Secundaria/métodos , Lactante , Recién Nacido , Inyecciones Intramusculares , Masculino , Papúa Nueva Guinea , Seguridad , Sensibilidad y EspecificidadRESUMEN
The burden and duration of asymptomatic malaria infections were measured in residents of the malaria endemic village of Gonoa, Madang Province, Papua New Guinea. Plasmodium falciparum, P. vivax and P. malariae infections in people aged 4 years to adulthood were compared. Frequent sampling at 3-day intervals for up to 61 days allowed assessment of individual episodes of infection. Statistical assessment of P. falciparum detection revealed a periodicity consistent with synchronous replication of this species over periods up to 27 days. The duration of P. falciparum episodes was longer across all age groups than that of P. vivax and P. malariae. A trend for decreasing duration with age was also noted in data from each species. This was most prominent in P. falciparum infections: median duration in 4-year-olds was > 48 days compared with a median between 9 and 15 days in older children and adults. The results are consistent with the slow acquisition of immunity to antigenically diverse Plasmodium populations and suggest a faster rate of acquisition to P. vivax and P. malariae than to P. falciparum.
Asunto(s)
Malaria/epidemiología , Plasmodium/aislamiento & purificación , Adolescente , Adulto , Factores de Edad , Animales , Niño , Preescolar , Humanos , Estudios Longitudinales , Malaria/sangre , Malaria/parasitología , Malaria Falciparum/sangre , Malaria Falciparum/epidemiología , Malaria Vivax/sangre , Malaria Vivax/epidemiología , Persona de Mediana Edad , Papúa Nueva Guinea/epidemiología , Parasitemia , Periodicidad , Plasmodium falciparum/aislamiento & purificación , Plasmodium malariae/aislamiento & purificación , Plasmodium vivax/aislamiento & purificación , Prevalencia , Especificidad de la Especie , Análisis de SupervivenciaRESUMEN
We describe the dynamics of co-infections of Plasmodium falciparum and P. vivax in 28 asymptomatic children by genotyping these species using the polymorphic loci Msp2 and Msp3alpha, respectively. The total number of Plasmodium spp. infections detected using 3 day sampling over 61 days varied between 1 and 14 (mean 6.6). The dynamics of P. falciparum and P. vivax genotypes varied greatly both within and amongst children. Periodicity in the detection of P. falciparum infections is consistent with the synchronous replication of individual genotypes. Replication synchrony of multiple co-infecting genotypes was not detected. In 4-year-old children P. falciparum genotype complexity was reduced and episodes lasted significantly longer (median duration > 60 days) when compared to children aged 5-14 years (median duration 9 days). P. vivax genotype complexity was not correlated with age but the episode duration was also longer for this species in 4-year-olds than in older children but was not as long as P. falciparum episodes. Recurrence of P. falciparum and P. vivax genotypes over weeks was observed. We interpret these major fluctuations in the density of genotypes over time as the result of the mechanism of antigenic variation thought to be present in these Plasmodium species.
Asunto(s)
Variación Genética/genética , Malaria Falciparum/complicaciones , Malaria Vivax/complicaciones , Plasmodium falciparum/genética , Plasmodium vivax/genética , Adolescente , Animales , Antígenos de Protozoos/química , Southern Blotting , Niño , Preescolar , Cartilla de ADN , ADN Protozoario/química , ADN Protozoario/aislamiento & purificación , Electroforesis en Gel de Agar , Femenino , Humanos , Estudios Longitudinales , Malaria Falciparum/epidemiología , Malaria Falciparum/parasitología , Malaria Vivax/epidemiología , Malaria Vivax/parasitología , Masculino , Papúa Nueva Guinea/epidemiología , Parasitemia/epidemiología , Parasitemia/parasitología , Plasmodium falciparum/química , Plasmodium vivax/química , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Proteínas Protozoarias/química , Análisis de Secuencia de ADN , Análisis de SupervivenciaRESUMEN
Chemotherapy-based eradication programs are aimed at stopping transmission of Wuchereria bancrofti by its obligatory mosquito vector. This study compares one year post-treatment W. bancrofti infection rates of Anopheles punctulatus, the main vector of lymphatic filariasis in Papua New Guinea, using traditional dissection techniques and a polymerase chain reaction (PCR)-based ELISA of a parasite-specific Ssp I repeat. A total of 633 mosquitoes in 35 batches were dissected. Six batches contained W. bancrofti-infected mosquitoes, giving a minimum infection rate of 0.9%. This value was not different than the actual infection rate, which was 9 (1.4%) of 633 mosquitoes (P = 0.48). The DNA was extracted from 47 pools containing a mean of 13.2 mosquitoes per pool. A total of 621 mosquitoes were processed for the PCR-ELISA, including 486 caught by human bait and 135 by light trap, which included both dead and live mosquitoes. Of 23 pools of alcohol-preserved human-bait mosquitoes, seven were positive by the PCR-ELISA, giving an infection rate identical to that obtained by dissection of individual mosquitoes (1.4%). The minimum infection rates for pools of light-trap mosquitoes found dead and alive were 2.7% (2 of 74) and 4.9% (3 of 61), respectively. These values did not differ from each other (P = 0.84), but the overall infection rate of light-trap mosquitoes was greater than that of mosquitoes captured by human bait (3.7% versus 1.4%; P = 0.09). These data indicate that the PCR-ELISA of a W. bancrofti Ssp I repeat using pools of mosquitoes is comparable to traditional dissection techniques for monitoring transmission intensity following introduction of mass chemotherapy. This approach may also be useful for rapid and cost-effective assessment of transmission in endemic areas where the frequency of overt lymphatic pathology is low.
Asunto(s)
Anopheles/parasitología , Filariasis/prevención & control , Reacción en Cadena de la Polimerasa , Wuchereria bancrofti/aislamiento & purificación , Animales , ADN de Helmintos/análisis , Ensayo de Inmunoadsorción Enzimática , Humanos , Papúa Nueva GuineaRESUMEN
Antigenic polymorphism and HLA restriction may limit the immunogenicity of a subunit vaccine against liver-stage Plasmodium falciparum. We examined 59 clinical isolates and five laboratory clones of P. falciparum for polymorphism in the N- and C-terminal regions of LSA-1, evaluated binding of the corresponding peptides to selected HLA class I alleles, and measured IFN-gamma responses in residents of a malaria-endemic area of Papua New Guinea where HLA-A*1101, -24, -B13, and -B40 are the most common class I alleles. LSA-1 polymorphism was limited to a single non-synonymous mutation encoding serine (S), proline (P), or threonine (T) at amino acid 85. Nine-mer 84-92 peptides with S, T, or P at the primary anchor position bound differentially to HLA-A11, -A2, and -B7. IFN-gamma ELISPOT responses increased with age in malaria-exposed subjects: 14-16% and 30-36% of 2-5- and 6-54-year-olds, respectively, had > or =10 IFN-gamma-secreting cells/106 peripheral blood mononuclear cells when stimulated with at least one peptide variant (P<0.05). IFN-gamma responses to all three peptides were also greater for older than younger individuals. No children < 3 years old had lymphocytes that responded to all three 84-92 peptides, whereas 45% of adults (mean age 48 years) had aggregated IFN-gamma responses. These data support the notion that age-related cumulative exposure to P. falciparum increases the frequency of IFN-gamma responses to polymorphic epitopes of liver-stage antigens such as LSA-1.
Asunto(s)
Envejecimiento/inmunología , Variación Antigénica/inmunología , Antígenos de Protozoos/inmunología , Epítopos de Linfocito T/inmunología , Antígenos HLA-A/inmunología , Antígenos HLA-B/inmunología , Interferón gamma/biosíntesis , Malaria Falciparum/inmunología , Adolescente , Adulto , Alelos , Animales , Variación Antigénica/genética , Antígenos de Protozoos/genética , Niño , Preescolar , Epítopos de Linfocito T/genética , Antígenos HLA-A/genética , Antígeno HLA-A11 , Antígeno HLA-A2/inmunología , Antígenos HLA-B/genética , Prueba de Histocompatibilidad , Humanos , Interferón gamma/inmunología , Malaria Falciparum/epidemiología , Malaria Falciparum/fisiopatología , Persona de Mediana Edad , Papúa Nueva Guinea/epidemiología , Péptidos/inmunología , Plasmodium falciparum/genética , Plasmodium falciparum/inmunologíaRESUMEN
The mosquito sampling efficiency of CDC (Centers for Disease Control) miniature light traps hung adjacent to mosquito nets, was compared with that of both indoor and outdoor human-bait collections in ten villages in the Wosera area of Papua New Guinea. The most frequently collected anopheline in the matched indoor and light trap samples was Anopheles koliensis Owen, followed by A. punctulatus Dönitz, A. karwari (James), A. farauti Laveran (sensu lato), A. longirostris Brug and A. bancroftii Giles. All species were much less frequent in the light traps than in landing catches. The hypothesis that the numbers of mosquitoes in light traps are proportional to human landing catches was examined using regression models that allowed for sampling error in both entomological measurements. Light traps under-sampled A. punctulatus and A. farauti s.l. at high densities. The models indicated that the ratio of light trap to landing catch females of A. koliensis and A. karwari increased with increasing mosquito density. Light trap catches of A. longirostris were proportional to indoor landing rates but when outdoor landing rates were high this species was under-sampled by light traps. Numbers of A. bancroftii in light traps were found to be proportional to those in outdoor landing catches, but were negatively related to those attempting to bite indoors. Circumsporozoite positivity rates for both Plasmodium falciparum Welch and P. vivax (Grassi & Feletti) in A. punctulatus and A. farauti s.l. were significantly higher in light trap collections than in either indoor or outdoor landing catches, suggesting that light traps may selectively sample older mosquitoes of these species.
Asunto(s)
Anopheles/clasificación , Insectos Vectores/clasificación , Animales , Anopheles/parasitología , Enfermedades Endémicas , Humanos , Insectos Vectores/parasitología , Malaria/epidemiología , Control de Mosquitos/métodos , Papúa Nueva Guinea/epidemiología , Plasmodium falciparum , Plasmodium malariae , Plasmodium vivax , Proteínas Protozoarias/análisisRESUMEN
The peopling of the Pacific was a complex sequence of events that is best reconstructed by reconciling insights from various disciplines. Here we analyze the human polyomavirus JC (JCV) in Highlanders of Papua New Guinea (PNG), in Austronesian-speaking Tolai people on the island of New Britain, and in nearby non-Austronesian-speaking Baining people. We also characterize JCV from the Chamorro of Guam, a Micronesian population. All JCV strains from PNG and Guam fall within the broad Asian group previously defined in the VP1 gene as Type 2 or Type 7, but the PNG strains were distinct from both genotypes. Among the Chamorro JCV samples, 8 strains (Guam-1) were like the Type 7 strains found in Southeast Asia, while nine strains (Guam-2) were distinct from both the mainland strains and most PNG strains. We identified three JCV variants within Papua New Guinea (PNG-1, PNG-2 and PNG-3), but none of the Southeast Asian (Type 7) strains. PNG-1 strains were present in all three populations (Highlanders and the Baining and Tolai of New Britain), but PNG-2 strains were restricted to the Highlanders. Their relative lack of DNA sequence variation suggests that they arose comparatively recently. The single PNG-3 strain, identified in an Austronesian-speaking Tolai individual, was closely related to the Chamorro variants (Guam-2), consistent with a common Austronesian ancestor. In PNG-2 variants a complex regulatory region mutation inserts a duplication into a nearby deletion, a change reminiscent of those seen in the brains of progressive multifocal leukoencephalopathy patients. This is the first instance of a complex JCV rearrangement circulating in a human population.