In vitro and in silico evaluation of fucosterol from Sargassum horridum as potential human acetylcholinesterase inhibitor.

The fucosterol has been reported numerous biological activities. In this study, the activity in vitro of the fucosterol from Sargassum horridum as potential human acetylcholinesterase inhibitor was evaluated. The structural identification was obtained by nuclear magnetic resonance (NMR) spectroscopy and based on experimental data, we combined docking and molecular dynamics simulations coupled to the molecular-mechanics-generalized-born-surface-area approach to evaluating the structural and energetic basis for the molecular recognition of fucosterol and neostigmine at the binding site of acetylcholinesterase (AChE). In addition, the Lineweaver-Burk plot showed the nature of a non-competitive inhibition. The maximum velocity (Vmax) and the constant of Michaelis-Menten (Km) estimated for fucosterol (0.006 µM) were 0.015 1/Vo (ΔA/h and 6.399 1/[ACh] mM-1, respectively. While, for neostigmine (0.14 µM), the Vmax was 0.022 1/Vo (ΔA/h) and Km of 6.726 1/[ACh] mM-1, these results showed a more effective inhibition by fucosterol respect to neostigmine. Structural analysis revealed that neostigmine reaches the AChE binding site reported elsewhere, whereas fucosterol can act as a no-competitive and competitive acetylcholinesterase inhibitor, in agree with kinetic enzymatic experiments. Binding free energy calculations revealed that fucosterol reaches the acetylcholinesterase binding site with higher affinity than neostigmine, which is according to experimental results. Whereas the per-residue decomposition free energy analysis let us identify crucial residues involved in the molecular recognition of ligands by AChE. Results corroborate the ability of theoretical methods to provide crucial information at the atomic level about energetic and structural differences in the binding interaction and affinity from fucosterol with AChE. Communicated by Ramaswamy H. Sarma.

Second isolated anastomotic recurrence after curative surgery for colorectal cancer.

Introduction The two mechanisms postulated for cancer recurrence at the anastomosis site ('anastomotic recurrence' (AR)) after curative surgery for colorectal cancer are: (i) intraluminal dissemination of viable cancer cells; (ii) metachronous carcinogenesis related with changes in the local milieu provoked by the materials employed to carry out the anastomosis. Case History We describe a 79-year-old female who underwent a left hemicolectomy due to a stenotic lesion shown on colonoscopy: an adenocarcinoma (pT3NO, G2). One year after surgery, control colonoscopy revealed an AR, so a new resection was carried out. Pathology showed it to be a recurrent adenocarcinoma over the staple line (pT3N0, G2). One year after the second surgical procedure, control colonoscopy evinced a new AR, resulting in a new resection. Pathology revealed a new AR. Conclusions This is only the second time that a second isolated AR after curative resection for colorectal cancer has been reported.

Incarcerated umbilical Littre's hernia at the trocar site of a previous laparoscopic surgical procedure.

INTRODUCTION: Prevalence of Littre's hernia (protrusion of a Meckel´s diverticulum through an opening in the abdominal wall) is very low, and Littre's hernias found in an umbilical site are uncommon. Even rarer are cases of an incarcerated hernia resulting in a surgical emergency. Trocar-site hernias are a relatively common complication after laparoscopic cholecystectomy that develop in association with insertion of wide trocars (usually at the umbilical port). CASE HISTORY: A 63-year-old female with a history of obesity, diabetes mellitus, hypertension, laparoscopic cholecystectomy and open hysterectomy arrived at hospital complaining of acute umbilical pain but with no other symptoms or fever. A painful mass observed was believed to be an incarcerated umbilical hernia at a trocar site used in previous laparoscopic surgery. Emergency surgery was undertaken: the opening of the hernia sac revealed a Meckel's diverticulum within it. The Meckel's diverticulum was resected using a stapler, followed by herniorrhaphy and hernioplasty. Postoperative recovery was uneventful. CONCLUSION: An incarcerated umbilical Littre's hernia at a laparoscopic trocar site has not been reported before. To avoid this complication, we agree with the numerous authors who recommend closure of trocar sites of width ≥10mm.

A safer disposal of hazardous phosphate coating sludge by formation of an amorphous calcium phosphate matrix.

Phosphate coating hazardous wastes originated from the automotive industry were efficiently encapsulated by an acid-base reaction between phosphates present in the sludge and calcium aluminate cement, yielding very inert and stable monolithic blocks of amorphous calcium phosphate (ACP). Two different compositions of industrial sludge were characterized and loaded in ratios ranging from 10 to 50 wt.%. Setting times and compressive strengths were recorded to establish the feasibility of this method to achieve a good handling and a safe landfilling of these samples. Short solidification periods were found and leaching tests showed an excellent retention for toxic metals (Zn, Ni, Cu, Cr and Mn) and for organic matter. Retentions over 99.9% for Zn and Mn were observed even for loadings as high as 50 wt.% of the wastes. The formation of ACP phase of low porosity and high stability accounted for the effective immobilization of the hazardous components of the wastes.

Treatment of toxic metal aqueous solutions: encapsulation in a phosphate-calcium aluminate matrix.

Polyphosphate-modified calcium aluminate cement matrices were prepared by using aqueous solutions polluted with toxic metals as mixing water to obtain waste-containing solid blocks with improved management and disposal. Synthetically contaminated waters containing either Pb or Cu or Zn were incorporated into phosphoaluminate cement mortars and the effects of the metal's presence on setting time and mechanical performance were assessed. Sorption and leaching tests were also executed and both retention and release patterns were investigated. For all three metals, high uptake capacities as well as percentages of retention larger than 99.9% were measured. Both Pb and Cu were seen to be largely compatible with this cementitious matrix, rendering the obtained blocks suitable for landfilling or for building purposes. However, Zn spoilt the compressive strength values because of its reaction with hydrogen phosphate anions, hindering the development of the binding matrix.

Air Polycyclic Aromatic Hydrocarbons (PAHs) associated with PM2.5 in a North Cantabric coast urban environment.

Health studies and more specifically epidemiological studies require an extended analysis of the physical and chemical characteristics of the environment in which are held. The aim of this study is to evaluate the concentration of six Polycyclic Aromatic Hydrocarbons (PAHs) in PM2.5 fraction of air in a peri-urban environment in the province of Gipuzkoa (Basque Country, Spain) where residential areas are surrounded by industrial activity. The six studied PAH are as follows: Fluoranthene, Benzo(b)fluoranthene, Benzo(k)fluoranthene, (Benzo(a)pyrene, Indene(123-cd)pyrene and Benzo(ghi)perylene. Our six-year study shows a decrease in PAH concentrations between 2006 and 2011, especially since 2008 due to the fall in industrial activity and related traffic. Overall, 801 data were obtained. Total PAH concentration ranged between 0.3 and 8.29ngm(-3) and Benzo(a)pyrene (BaP) from 0.05 to 0.88ngm(-3). The mean value for BaP in PM2.5 was 0.15ngm(-3) and the target value established by European legislation in PM10 was only exceeded in occasional days. Contribution percentages of each PAH in the monitoring sites were very similar, indicating common sources. The results of this study suggest that emission from industry play an important role although we also have to consider the contribution of traffic. PAH seasonal variations are similar as those reported in many previous studies. BaP and PAH concentration values in our region of study were in the range of other Spanish cities.

Solidification/stabilization of toxic metals in calcium aluminate cement matrices.

The ability of calcium aluminate cement (CAC) to encapsulate toxic metals (Pb, Zn and Cu) was assessed under two curing conditions. Changes in the consistency and in the setting time were found upon the addition of the nitrates of the target metals. Both Pb and Cu caused a delay in CAC hydration, while Zn accelerated the stiffening of the mortar. Compressive strengths of the metal-doped mortars, when initially cured at 60 °C/100% RH, were comparable with that of the free-metal mortar. Three different pore size distribution patterns were identified and related to the compounds identified by XRD and SEM. Sorbent capacities of CAC for the toxic metals were excellent: a total uptake was achieved for up to 3 wt.% loading of the three metals. In this way, CAC mortars were perfectly able to encapsulate the toxic metals, allowing the use of CAC for waste management as proved by the leaching tests.

Encapsulation, solid-phases identification and leaching of toxic metals in cement systems modified by natural biodegradable polymers.

Cement mortars loaded with Cr, Pb and Zn were modified by polymeric admixtures [chitosans with low (LMWCH), medium (MMWCH) and high (HMWCH) molecular weight and hydroxypropylchitosan (HPCH)]. The influence of the simultaneous presence of the heavy metal and the polymeric additive on the fresh properties (consistency, water retention and setting time) and on the compressive strength of the mortars was assessed. Leaching patterns as well as properties of the cement mortars were related to the heavy metals-bearing solid phases. Chitosan admixtures lessened the effect of the addition of Cr and Pb on the setting time. In all instances, chitosans improved the compressive strength of the Zn-bearing mortars yielding values as high as 15 N mm(-2). A newly reported Zn phase, dietrichite (ZnAl(2)(SO(4))(4)·22H(2)O) was identified under the presence of LMWCH: it was responsible for an improvement by 24% in Zn retention. Lead-bearing silicates, such as plumalsite (Pb(4)Al(2)(SiO(3))(7)), were also identified by XRD confirming that Pb was mainly retained as a part of the silicate network after Ca ion exchange. Also, the presence of polymer induced the appearance and stabilization of some Pb(IV) species. Finally, diverse chromate species were identified and related to the larger leaching values of Cr(VI).

Interaction of carboxymethylchitosan and heavy metals in cement media.

The performance of an etherified chitosan, carboxymethylchitosan (CMCH), when added to cement mortars doped with heavy metals, was assessed. In the presence of heavy metals (Cr, Pb, Zn) strong modifications of the fresh-state properties were evaluated. The addition of the polymer was seen to be useful in minimising some of these modifications, as those related to the setting time. A competitive mechanism for adsorption between the oxoanionic form of the metals and the carboxylate groups of the chitosan derivative was established. Studies on the metal chelating ability of the polymer and leaching from the hardened specimens showed scarce complexation under alkaline conditions, pointing to physical entrapment based on metal adsorption. However, significant chelation of metals was proved at near-neutral pH, suggesting the potential usefulness of the polymer as an agent for removing heavy metals from polluted waters and subsequently immobilizing them in cement mortars. Leaching tests carried out on polymer-metal complex-bearing samples showed a reduction in the amount of released Pb and Zn.

Review: leucocyte-endothelial cell crosstalk at the blood-brain barrier: a prerequisite for successful immune cell entry to the brain.

Leucocyte migration into the central nervous system is a key stage in the development of multiple sclerosis. While much has been learnt regarding the sequential steps of leucocyte capture, adhesion and migration across the vasculature, the molecular basis of leucocyte extravasation is only just being unravelled. It is now recognized that bidirectional crosstalk between the immune cell and endothelium is an essential element in mediating diapedesis during both normal immune surveillance and under inflammatory conditions. The induction of various signalling networks, through engagement of cell surface molecules such as integrins on the leucocyte and immunoglobulin superfamily cell adhesion molecules on the endothelial cell, play a major role in determining the pattern and route of leucocyte emigration. In this review we discuss the extent of our knowledge regarding leucocyte migration across the blood-brain barrier and in particular the endothelial cell signalling pathways contributing to this process.

Screening of extracts of algae from Baja California sur, Mexico as reversers of the antibiotic resistance of some pathogenic bacteria.

BACKGROUND AND OBJECTIVES: Sixty ethanol extracts of marine flora of Baja California Sur (Mexico) were screened to evaluate the reversing effect of the bacterial resistance to antibiotics in combination with a sublethal concentration of ampicillin or erythromycin. MATERIALS AND METHODS: The activity was assayed by using a modification of the classical agar-diffusion method against 3 resistant, pathogenic bacteria; Escherichia coil (ATCC BAA196), Staphylococcus aureus (ATCC BAA42), and Streptococcus pyogenes (ATCC BAA946). RESULTS: From the 60 ethanolic extracts, 12 (20%) of them in combination with ampicillin were able to reverse the resistance of Staphylococcus aureus and 8 (13%) with erythromycin yielded the same reversal with Streptococcus pyogenes. An extract from Sargassum horridum was the only one that reversed the resistance to antibiotics against both Staphylococcus aureus and Streptococcus pyogenes. CONCLUSIONS: Our findings suggest that some algae may be source of compounds with the potential to reverse the antibiotic resistance of some bacteria. In addition, of the assayed extracts, 35 (57%) showed inhibitory activity against Staphylococcus aureus, 48 (78%) were active against Streptococcus pyogenes, but none was active against Escherichia coil. The most active extracts were from Laurencia spp., Gelidium robustum, Chnoospora implexa, Padina mexicana, Gracilaria subsecundata, and Dictyopteris undulata.

Development and mapping of a codominant SCAR marker linked to the andromonoecious gene of melon.

Monoecy is an important goal for melon breeding because of the agronomic advantages it provides to parental lines in that they do not require hand emasculation to develop monoecious F1 hybrids, the latter producing fruits of higher quality. Monoecious phenotype is conferred by the dominant allele of the andromonoecious (a) gene, whereas recessive homozygous plants are andromonoecious. A bulked segregant analysis (BSA) approach performed in a set of 38 double-haploid lines has allowed us to identify an AFLP marker linked to the a gene at 3.3 cM. Following cloning and sequencing of the AFLP fragment, specific PCR primers were designed and used in the amplification of a codominant SCAR marker. Using a backcrossed mapping population of 530 plants, the SCAR marker could be mapped near the a locus (5.5 cM). Size difference between the two allelic SCAR fragments is 42 bp and might be due to a deletion/insertion. The SCAR marker is closest to the a gene identified to date, and can be useful in breeding programs, using marker-assisted selection procedures to screen for sexual types in melon.

[Infantile dyspnea due to pyogenic laryngeal granuloma]. / Disnea infantil por "granuloma piogénico" laríngeo.

Two appearances are outstanding in this case report. First is the clinical picture of chronic laryngeal stridor of a child due to an acquired haemangioma. And that the anatomopathologic subtype of angioma is a pyogenic granuloma noteworthy, as we shall see, the use of the term "pyogenic granuloma" based only on anatomopathological findings in order to circumscribe this lesion.

Granuloma formation and parasite disintegration in porcine cysticercosis: comparison with human neurocysticercosis.

Taenia solium cysticerci infect human beings and pigs, causing cysticercosis. In this study the pig was used as a model to characterize the immune response against cysticerci, given the difficulties in analysing the developing immune response in infected human brains. Metacestodes in different stages of viability or degeneration were isolated from the brain, heart and skeletal muscle of naturally infected swine, and the adjacent tissue was examined histologically. The immune response elicited by the cysticerci was classified into four separate stages. In stage I the parasites were surrounded by a thin layer of collagen type I, and by stage II there was a sparse inflammatory infiltrate. In stage III, granuloma formation was evident, and by stage IV the parasite was surrounded by an eosinophil-rich infiltrate and its vesicular membrane had begun to degenerate. The final stage, IV, was detected mainly in the heart but not in the brain. The granulomatous reaction in swine resembled that described previously in human patients, but differed in the abundance of eosinophils, the relative paucity of plasma cells, and the discrete deposition of collagen. These differences were probably due to the fact that in pigs the immune response can be examined earlier than in human patients, in whom sampling is inevitably made at a more chronic stage.

The human nervous tissue in proximity to granulomatous lesions induced by Taenia solium metacestodes displays an active response.

In neurocysticercosis, the nervous tissue surrounding the brain lesion is affected as a consequence of the local immune response induced by a Taenia solium metacestode. In this study, a histological and immunohistochemical analysis of five brain specimens from patients with neurocysticercosis revealed a proinflammatory activity reflected by an apparently altered blood-brain barrier permeability, secretion of pro-inflammatory cytokines, and up-regulation of molecules associated with antigen presentation. There were also anti-inflammatory cytokines, as well as an active wound-healing process reflected by angiogenesis, collagen deposition and glial scar formation. This immune response displayed by the nervous tissue adjacent to chronic neurocysticercosis lesions appeared to be contributing to the local tissue damage, and hence, may be fundamental in the pathology of NCC.

Laparoscopic appendectomy by ultrasonically activated scalpel in acute appendicitis: preliminary report.

BACKGROUND AND PURPOSE: Laparoscopic appendectomy (LA) is increasingly being used in treating acute appendicitis. New instruments such as the ultrasonically activated scalpel (UAS) have been introduced for most laparoscopic procedures. We evaluated the use of UAS in the performance of LA, as the potential of this instrument in this type of surgery remains to be defined. PATIENTS AND METHODS: Three patients with acute right lower abdominal pain were managed by the laparoscopic approach. Once the diagnosis of acute appendicitis was established, laparoscopic appendectomy was performed with the UAS. RESULTS: The mean operative time was 42.3 minutes (range 32-49 minutes). There were no complications related to the treatment with UAS of either the vascular pedicle or the appendicecal stump. No electrosurgical coagulation, clips, loops, or endostapler was used in any patient. CONCLUSIONS: Total LA performed with UAS is feasible. Use of the UAS may make dissection and resection of the appendix easier, helping to reduce the mean operative time.

Brain granulomas in neurocysticercosis patients are associated with a Th1 and Th2 profile.

Neurocysticercosis (NCC) is a common central nervous system (CNS) infection caused by Taenia solium metacestodes. Despite the well-documented importance of the granulomatous response in the pathogenesis of this infection, there is limited information about the types of cells and cytokines involved. In fact, there has been limited characterization of human brain granulomas with any infectious agent. In the present study a detailed histological and immunohistochemical analysis of the immune response was performed on eight craniotomy specimens where a granuloma surrounded each T. solium metacestode. The results indicated that in all the specimens there was a dying parasite surrounded by a mature granuloma with associated fibrosis, angiogenesis, and an inflammatory infiltrate. The most abundant cell types were plasma cells, B and T lymphocytes, macrophages, and mast cells. Th1 cytokines were prevalent and included gamma interferon, interleukin-18 (IL-18), and the immunosuppressive, fibrosis-promoting cytokine transforming growth factor beta. The Th2 cytokines IL-4, IL-13, and IL-10 were also present. These observations indicate that a chronic immune response is elicited in the CNS environment with multiple cell types that together secrete inflammatory and anti-inflammatory cytokines. In addition, both collagen type I and type III deposits were evident and could contribute to irreversible nervous tissue damage in NCC patients.

Tuberculosis genital complicada / Complicated genital tuberculosis

La tuberculosis genital es una enfermedad actualmente de rara presentación en nuestro medio. Esta eventualidad, asociada a lo inespecífico de sus manifestaciones clínicas, hacen que sea una patología de difícil diagnóstico. Suele ser un hallazgo en el curso de una laparotomía realizada con otro motivo, o bien puede interpretarse y tratarse erróneamente como una enfermedad pélvica inflamatoria, como en el caso que presentamos, a cuyo diagnóstico se llegó tras una evolución tórpida de un proceso inflamatorio anexial (AU)

Eventraciones y hernias ventrales: experiencia con 200 casos por abordaje laparoscópico. Técnica quirúrgica / Laparoscopic approach in abdominal wall and incisional hernias: experience in 200 cases and surgical technique

Objetivo. El tratamiento laparoscópico de eventraciones y hernias ventrales ha sido poco usado, aunque estas hernias se pueden tratar muy bien por vía laparoscópica. El objetivo de este estudio ha sido revisar nuestra experiencia en el abordaje laparoscópico para el tratamiento de las hernias ventrales e incisionales. Material y métodos. Entre enero de 1994 y noviembre de 1999 una serie de 200 pacientes fueron intervenidos por hernias ventrales e incisionales por medio de cirugía laparoscópica con una media de 36 meses de seguimiento postoperatorio. La media del número de defectos de pared fue de 4,3 por paciente y la superficie media a corregir de 138 cm2. Un total de 15 hernias fueron menores de 5 cm, 105 de tamaño medio (5 a 10 cm) y 80 fueron hernias grandes (> 10 cm). El origen de los defectos de pared fue primario en 33 casos y posquirúrgico en 167. Se usaron tres puertas de entrada en todas ellas describiendo el resto de la técnica quirúrgica. Los defectos herniarios fueron cubiertos con malla de PTFE Dual Mesh de 26 × 18 cm en 10 pacientes, 19 × 15 en 187 y 10 × 15 en 65 casos. En 65 pacientes se usó una malla adicional. En los últimos 130 casos fue empleada una malla PTFE Dual Mesh Plus multiperforada. Resultados. El tiempo medio quirúrgico fue de 64 min. Sólo una intervención fue reconvertida a cirugía abierta (0,5 por ciento) y únicamente un paciente requirió una segunda intervención en el postoperatorio inmediato. Las complicaciones menores incluyeron 2 pacientes con enfisema subcutáneo, 4 casos con hematomas subcutáneos, 28 pacientes con pequeños seromas no complicados y 16 casos que refirieron dolor postoperatorio. No hubo infecciones en los orificios de los trocares ni tampoco infecciones de ninguna malla. Cuatro pacientes desarrollaron hernia recidivada (2 por ciento) en el primer mes postoperatorio y fueron reintervenidos con una técnica laparoscópica similar. La ingesta oral y la movilización se realizó a las pocas horas de la cirugía con una estancia media hospitalaria de 28 h. Conclusiones. La técnica laparoscópica permite evitar grandes incisiones, así como la no utilización de drenajes con un bajo número de complicaciones, infecciones y recidivas. El abordaje laparoscópico acorta considerablemente el tiempo quirúrgico y la estancia hospitalaria postoperatoria (AU)