Asunto(s)
Biomarcadores de Tumor/análisis , Ciclosporina/uso terapéutico , Inmunosupresores/uso terapéutico , Linfoma Cutáneo de Células T/tratamiento farmacológico , Factores de Transcripción NFATC/análisis , Paniculitis/tratamiento farmacológico , Adolescente , Adulto , Anciano , Femenino , Humanos , Linfoma Cutáneo de Células T/química , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
BACKGROUND: Necrotising non-granulomatous lymphadenitis can be observed in several conditions, most notably infection (including tuberculosis, yersiniosis and nocardiasis), Kikuchi-Fujimoto disease and systemic lupus erythematosus. AIMS: To evaluate the role of PCR in the detection of Mycobacterium tuberculosis in necrotising non-granulomatous lymphadenitis in Thai patients using formalin-fixed paraffin-embedded tissue. METHODS: 35 patient samples showing necrotising non-granulomatous lymphadenitis were subjected to PCR for detection of the IS6110 sequence of M tuberculosis. For comparison, sections were visually assessed for acid-fast bacilli using the Ziehl-Neelsen stain. RESULTS: Among 35 cases of necrotising non-granulomatous lymphadenitis, a conclusive diagnosis could be reached in 23 cases: 15 cases of Kikuchi-Fujimoto disease, 6 of tuberculosis and 2 of systemic lupus erythematosus. Of the 6 cases of tuberculous lymphadenitis, 4 (66.6%) were detected by PCR in formalin-fixed paraffin-embedded tissue samples. PCR was positive in 6/12 of the remaining cases (50%) in which a definitive diagnosis could not be reached by other methods. CONCLUSION: Using PCR, a significant percentage (28%) of cases of necrotising non-granulomatous lymphadenitis in this study could be attributed to M tuberculosis. PCR for identification of the organism can be extremely helpful in confirming a diagnosis of tuberculosis when Ziehl-Neelsen staining is negative.
Asunto(s)
Mycobacterium tuberculosis/aislamiento & purificación , Tuberculosis Ganglionar/diagnóstico , Adolescente , Adulto , Biopsia , ADN Bacteriano/aislamiento & purificación , Femenino , Formaldehído , Humanos , Ganglios Linfáticos/patología , Masculino , Persona de Mediana Edad , Mycobacterium tuberculosis/genética , Necrosis , Neutrófilos/patología , Adhesión en Parafina , Reacción en Cadena de la Polimerasa/métodos , Sensibilidad y Especificidad , Tuberculosis Ganglionar/patología , Adulto JovenAsunto(s)
Síndrome de Inmunodeficiencia Adquirida/complicaciones , Neoplasias del Sistema Nervioso Central/patología , Linfoma de Células B Grandes Difuso/patología , Síndrome de Inmunodeficiencia Adquirida/tratamiento farmacológico , Adulto , Terapia Antirretroviral Altamente Activa , Encéfalo/metabolismo , Encéfalo/patología , Neoplasias del Sistema Nervioso Central/complicaciones , Neoplasias del Sistema Nervioso Central/metabolismo , Humanos , Inmunohistoquímica , Linfoma de Células B Grandes Difuso/complicaciones , Linfoma de Células B Grandes Difuso/metabolismo , MasculinoRESUMEN
Although several recurrent genetic aberrations are known to occur in MALT lymphoma, no comprehensive study on the most prevalent MALT lymphoma-associated genetic aberrations is available. We therefore screened 252 primary MALT lymphomas for translocations t(11;18)(q21;q21), t(14;18)(q32;q21), and t(1;14)(p22;q32), and trisomies 3 and 18. The above-listed translocations occurred mutually exclusively and were detected overall in 13.5, 10.8, and 1.6% of the cases; trisomy 3 and/or 18 occurred in 42.1%. The frequency at which the translocations occurred varied markedly with the primary site of disease. The t(11;18)(q21;q21) was mainly detected in pulmonary and gastric tumors, whereas the t(14;18)(q32;q21) was most commonly found in lesions of the ocular adnexa/orbit, skin, and salivary glands. Trisomies 3 and 18 each occurred most frequently in intestinal and salivary gland MALT lymphomas. Our results demonstrate that the three translocations and trisomies 3 and 18 occur at markedly variable frequencies in MALT lymphoma of different sites.