RESUMEN
The patterns of RNA from cells infected with avian adenovirus chicken embryo lethal orphan (CELO) virus were analyzed by Northern hybridization method. Early RNAs are specifically hybridized with several fragments of the Eco-RI restriction map: A (49-81%), B (0.2-20.3%), D (81-92.5%), and E fragments of the Bam-HI CELO DNA restriction map. Early RNAs were not hybridized with C (37.8-49%), G (31-37.8%), and E (20.3-31%) fragments of the Eco-RI DNA CELO restriction map. At least 19 types of virus-specific RNA molecules homologous to 4 different regions of the virus genome are produced in CELO-infected permissive cells. Several classes of virus-specific RNAs were identified in CELO virus-transformed cell strains.
Asunto(s)
Aviadenovirus/genética , Genoma Viral , Transcripción Genética , Animales , Northern Blotting , Células Cultivadas , Embrión de Pollo , ARN Mensajero/genética , Mapeo RestrictivoRESUMEN
A nondefective recombinant human adenovirus 5 (Ad5) carrying the gene encoding for the porcine rotavirus outer capsid protein VP7 in the E3 region of the Ad5 genome has been obtained. mRNA for VP7 in recombinant Ad5KV14/VP7 infected cells was demonstrated by means of RT-PCR. Radioimmunoprecipitation of cell extracts infected with Ad5KV14/VP7 on early (12 h p.i.) and late (24 h p.i.) stages of adenovirus infection with rabbit polyclonal antiserum raised to purified rotavirus virions revealed a recombinant protein possessing the same electrophoretic mobility, 37 kDa, as the native rotavirus K VP7. One-dimensional peptide mapping also demonstrated the identity of the recombinant and native VP7.