RESUMEN
HrpZ, a type three secretion system helper protein from the plant-pathogen Pseudomonas syringae, can be recognized by many plants as a defence elicitor. Responses of Arabidopsis thaliana suspension cells to different HrpZ variants were studied by electrophysiological methods and cell death assay. Purified HrpZ originating from a compatible pathogen P. syringae pv. tomato DC3000 (HrpZ(Pto)) and incompatible P. syringae pv. phaseolicola (HrpZ(Pph)) both promoted Arabidopsis cell death. As an early response, both HrpZ variants induced an increase in time dependent K(+) outward rectifying current. In contrast, the effects of HrpZ proteins on anion currents were different: HrpZ(Pph) had no effect, and HrpZ(Pto) induced an anion current increase. This suggests that the observed responses of the K(+) channels and anion channels resulted from different and separable interactions and that the interaction implied in anion current modulation is host-specific. HrpZ(Pto) and HrpZ(Pph) also had a different sequence preference in phage display screen for peptide-binding. These peptides presumably represent a part of a putative target protein in the host, and HrpZ proteins of different P. syringae pathovars might have different binding specificities to match the allelic variation between plant species. Supporting the idea that the peptide-binding region of HrpZ is important for interactions with host cell components, we found that a mutation in that region changed the anion channel response of Arabidopsis cells.
Asunto(s)
Arabidopsis/microbiología , Proteínas de la Membrana Bacteriana Externa/metabolismo , Interacciones Huésped-Patógeno , Células Vegetales/metabolismo , Canales de Potasio/metabolismo , Pseudomonas syringae/patogenicidad , Alelos , Arabidopsis/citología , Arabidopsis/fisiología , Proteínas de la Membrana Bacteriana Externa/genética , Muerte Celular , Células Cultivadas , Fenómenos Electrofisiológicos , Mutación , Biblioteca de Péptidos , Células Vegetales/microbiología , Unión Proteica , Pseudomonas syringae/genética , Especificidad de la Especie , Especificidad por Sustrato , Factores de TiempoRESUMEN
We report the direct observation of the nonreciprocity of the velocity of light, induced by electric and magnetic fields. This bilinear magneto-electro-optical effect appears in crossed electric and magnetic fields perpendicular to the light wave vector, as a refractive index difference between two counterpropagating directions. Using a high finesse ring cavity, we have measured this magnetoelectric nonreciprocity in molecular nitrogen at ambient temperature and atmospheric pressure; for light polarized parallel to the magnetic field it is 2η(â¥exp)(N2) = (4.7±1)×10(-23) m V(-1) T(-1) for λ = 1064 nm, in agreement with the expected order of magnitude. Our measurement opens the way to a deeper insight into light-matter interaction beyond the electric dipole approximation. We were able to measure a nonreciprocity as small as Δn =(5±2)×10(-18), which makes its observation in quantum vacuum a conceivable challenge.
RESUMEN
In the framework of experimental phonetics, our approach to the study of speech production is based on the measurement, the analysis and the modeling of orofacial articulators such as the jaw, the face and the lips, the tongue or the velum. Therefore, we present in this article experimental techniques that allow characterising the shape and movement of speech articulators (static and dynamic MRI, computed tomodensitometry, electromagnetic articulography, video recording). We then describe the linear models of the various organs that we can elaborate from speaker-specific articulatory data. We show that these models, that exhibit a good geometrical resolution, can be controlled from articulatory data with a good temporal resolution and can thus permit the reconstruction of high quality animation of the articulators. These models, that we have integrated in a virtual talking head, can produce augmented audiovisual speech. In this framework, we have assessed the natural tongue reading capabilities of human subjects by means of audiovisual perception tests. We conclude by suggesting a number of other applications of talking heads.
Asunto(s)
Cara/fisiología , Maxilares/fisiología , Modelos Lineales , Labio/fisiología , Paladar Blando/fisiología , Fonética , Lengua/fisiología , Interfaz Usuario-Computador , Humanos , Acústica del Lenguaje , Inteligibilidad del Habla/fisiología , Percepción del Habla/fisiología , Medición de la Producción del Habla/métodosRESUMEN
Affinity purified, freshly isolated CD34+ progenitors were shown to express low levels of type I interferon (IFN) receptors (740 +/- 60 binding sites/cell, K(d) 0.7 +/- 0.04 nM) determined by Scatchard's analysis using a radiolabelled, neutralizing, monoclonal antibody directed against the IFNAR1 chain of the human type I IFN receptor. Treatment of freshly isolated (day 0), highly purified (>95% pure) CD34+ cells with recombinant IFN-alpha resulted in rapid tyrosine phosphorylation and activation of STAT1, Tyk2 and JAK1 as shown by Western immunoblotting. Similarly, IFN treatment was shown by confocal microscopy to result in rapid nuclear localization of the transcription factors IRF1 and STAT2, demonstrating the presence of functional IFN receptors on freshly isolated (day 0) CD34+ cells. The number of specific type I IFN receptor binding sites expressed on hematopoietic progenitor cells increased to some 1440 +/- 40 per cell after 11 days of cultivation of CD34+ cells in vitrosuggesting that receptor expression increases with cell differentiation. IFN-mediated signal transduction and the inhibitory effect of IFN-alpha on 7 or 14 days CFU-GM and BFU-E colony formation was abrogated in the presence of the anti-IFNAR1 mAb, indicating that IFN-alpha acts directly on the proliferation of human hematopoietic progenitor cells via receptor activated signal transduction without excluding the induction of other cytokines or growth factors by residual accessory cells.
Asunto(s)
Antígenos CD34/análisis , Células Madre Hematopoyéticas/metabolismo , Receptores de Interferón/fisiología , Transducción de Señal , Transporte Activo de Núcleo Celular , Anticuerpos Monoclonales/farmacología , División Celular/efectos de los fármacos , Núcleo Celular/metabolismo , Células Cultivadas , Proteínas de Unión al ADN/metabolismo , Células Madre Hematopoyéticas/química , Humanos , Factor 1 Regulador del Interferón , Interferón-alfa/antagonistas & inhibidores , Janus Quinasa 1 , Cinética , Proteínas de la Membrana , Fosfoproteínas/metabolismo , Fosforilación/efectos de los fármacos , Proteínas Tirosina Quinasas/metabolismo , Proteínas/metabolismo , Receptor de Interferón alfa y beta , Receptores de Interferón/antagonistas & inhibidores , Receptores de Interferón/inmunología , Factor de Transcripción STAT1 , Factor de Transcripción STAT2 , TYK2 Quinasa , Transactivadores/metabolismoRESUMEN
The following contribution addresses several issues concerning speech degrees of freedom in French oral vowels, stop, and fricative consonants based on an analysis of tongue and lip shapes extracted from cineradio- and labio-films. The midsagittal tongue shapes have been submitted to a linear decomposition where some of the loading factors were selected such as jaw and larynx position while four other components were derived from principal component analysis (PCA). For the lips, in addition to the more traditional protrusion and opening components, a supplementary component was extracted to explain the upward movement of both the upper and lower lips in [v] production. A linear articulatory model was developed; the six tongue degrees of freedom were used as the articulatory control parameters of the midsagittal tongue contours and explained 96% of the tongue data variance. These control parameters were also used to specify the frontal lip width dimension derived from the labio-film front views. Finally, this model was complemented by a conversion model going from the midsagittal to the area function, based on a fitting of the midsagittal distances and the formant frequencies for both vowels and consonants.
Asunto(s)
Cinerradiografía/métodos , Maxilares/fisiología , Modelos Lineales , Labio/diagnóstico por imagen , Labio/fisiología , Modelos Biológicos , Medición de la Producción del Habla/métodos , Habla/fisiología , Humanos , Maxilares/diagnóstico por imagen , Fonética , Acústica del Lenguaje , Lengua/anatomía & histología , Lengua/diagnóstico por imagen , Lengua/fisiologíaRESUMEN
Interferons (IFNs) in common with other cytokines activate Janus tyrosine kinases and latent STAT transcription factors upon binding to their cell surface receptor. Type I IFNs bind to a receptor composed of two transmembrane polypeptides, IFNAR1 and IFNAR2, which belong to the class II cytokine receptor family that also includes the cellular receptors for IFN-gamma, interleukin-10 and coagulation protease factor VII (tissue factor). The extracellular domain of the type I IFN receptor chain IFNAR1, has four fibronectin type-III sub-domains. Human IFNAR1 has intrinsic weak affinity for type I IFNs and plays an essential role in transmembrane signaling, formation of a high affinity complex with IFN and the modulation of ligand specificity. In order to characterise the ligand binding site on IFNAR1 we analysed the epitope recognized by the anti-IFNAR1 mAb, 64G12, which inhibits the binding and biological activities of both IFN-alpha and IFN-beta. The target peptide recognized by the 64G12 mAb was determined by screening a set of 48 overlapping peptides covering the first two subdomains (residues 23-229) of the extracellular region of IFNAR1. The results of this study show that the peptide (FSSLKLNVY), localized within the first sub-domain (residues 89-97) of IFNAR1, which is recognized by the 64G12 mAb, most likely overlaps a site to which both IFN-alpha and IFN-beta bind in the ligand-receptor complex. Thus, since the 64G12 mAb can neutralize the biological activities of all the type I IFNs tested, we suggest that the target peptide recognized by the 64G12 mAb, is a possible anchorage point on IFNAR1, common to binding of both IFN-alpha and IFN-beta.
Asunto(s)
Mapeo Epitopo , Interferón Tipo I/metabolismo , Oligopéptidos/metabolismo , Receptores de Interferón/metabolismo , Secuencia de Aminoácidos , Animales , Anticuerpos Monoclonales/inmunología , Células CHO , Células COS , Bovinos , Cricetinae , Proteínas de Unión al ADN/metabolismo , Humanos , Fragmentos Fab de Inmunoglobulinas/inmunología , Interferón Tipo I/inmunología , Interferón-alfa/metabolismo , Interferón-alfa/farmacología , Janus Quinasa 1 , Proteínas de la Membrana , Datos de Secuencia Molecular , Oligopéptidos/inmunología , Fosforilación , Fosfotirosina/metabolismo , Proteínas Tirosina Quinasas/metabolismo , Receptor de Interferón alfa y beta , Receptores de Interferón/inmunología , Proteínas Recombinantes de Fusión/inmunología , Proteínas Recombinantes/inmunología , Factor de Transcripción STAT1 , Factor de Transcripción STAT2 , Transducción de Señal , Transactivadores/metabolismo , Células Tumorales Cultivadas , Virus de la Estomatitis Vesicular Indiana/crecimiento & desarrollo , Replicación ViralRESUMEN
The interaction of oestrogens with their intra-nuclear receptor is now recognized in its tri-dimensional aspects. A new receptor, ER beta, has been cloned and new non-genomic oestrogen effects have been reported. Hence, a better understanding of physiological mechanisms or of pathophysiological aspects, such as phyto- and xeno-oestrogens' influence on the organism, is possible. New compounds, belonging to the SERM family, are being explored. Raloxifene is now available for the prevention of post-menopausal osteoporosis. Tamoxifen has been associated with a beneficial effect for the primary prevention of breast cancer in a high-risk population. The understanding of genetic susceptibility could contribute, to better define women at risk. A dedicated primary prevention strategy in women with a high risk of breast cancer coupled with early detection through mammography will continue to improve the prognosis of this hormono-dependent cancer.
Asunto(s)
Sistema Endocrino/fisiología , Estrógenos/fisiología , Receptores de Estrógenos/fisiología , Animales , Neoplasias de la Mama/genética , Humanos , Receptores de Estrógenos/efectos de los fármacos , Receptores de Estrógenos/genéticaRESUMEN
OBJECTIVES: To assess the association between dietary fat and various urinary risk factors of calcium stone disease in a group of calcium stoneformers attending an outpatient stone clinic. METHODS: Mean daily fat intake from self-recorded 4-day dietary food records and mean 24-hour urinary risk factors from two separate collections were evaluated in 476 patients selected randomly from an adult population attending an outpatient stone clinic for the first time. RESULTS: Mean daily total fat intake for men and women was significantly different at 105.6 and 78.1 g, respectively. Examination of the relationship between total fat intake and 24-hour urinary volume, pH, and excretions of magnesium, citrate, oxalate, calcium, and uric acid revealed no significant regressions in men and only a weak association between fat intake and urinary uric acid in women. CONCLUSIONS: Dietary fat does not have a significant effect on the urinary risk factors of calcium stone disease.
Asunto(s)
Calcio , Grasas de la Dieta/efectos adversos , Cálculos Urinarios/epidemiología , Cálculos Urinarios/etiología , Adolescente , Adulto , Anciano , Calcio/análisis , Femenino , Humanos , Masculino , Persona de Mediana Edad , Análisis de Regresión , Factores de Riesgo , Cálculos Urinarios/químicaRESUMEN
We studied the parameters (latency, amplitude, peak velocity) of horizontal saccades in 32 patients with multiple sclerosis (MS) and 20 healthy, age matched control subjects. Saccades were recorded by direct-current electro-oculography technique (EOG). The patients were divided in 2 groups according to the absence or the presence of clinical internuclear ophthalmoplegia (INO). In both groups, we found increased latency, hypometria and reduced velocity. The disconjugacy of saccades was measured by calculating the ratio of abduction and adduction peak velocities (the versional disconjugacy index: VDI). Though the absolute value of this index might be dependent on the recording technique, its variation is not. Interestingly, the VDI was significantly increased in the groups of MS patients without clinical INO, indicating a more severe slowing in adduction. We concluded that VDI may be a very useful index in detecting subtle disorders in saccades conjugacy.
Asunto(s)
Esclerosis Múltiple/fisiopatología , Oftalmoplejía/fisiopatología , Movimientos Sacádicos/fisiología , Electrooculografía , Humanos , Esclerosis Múltiple/complicaciones , Oftalmoplejía/diagnóstico , Oftalmoplejía/etiología , Tiempo de ReacciónRESUMEN
Among 40 evaluable patients with metastatic renal cell cancer treated by high-dose interferon alpha-2a in combination with vinblastine, the authors have observed a 42.5% response rate. The 8 months' median duration of remissions is relatively short but some patients experience very long remissions, lasting many months after discontinuation of therapy. The survival of responding patients is significantly longer than the survival of nonresponders. The diverging results of other studies of the same combination are discussed; its efficacy is certainly dependent on the type of interferon, on the dose actually administered, and on the compliance to this often poorly tolerated treatment.
Asunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Carcinoma de Células Renales/tratamiento farmacológico , Interferón gamma/administración & dosificación , Neoplasias Renales/tratamiento farmacológico , Vinblastina/administración & dosificación , Adulto , Anciano , Carcinoma de Células Renales/mortalidad , Femenino , Humanos , Neoplasias Renales/mortalidad , Masculino , Persona de Mediana Edad , Metástasis de la Neoplasia , Proteínas RecombinantesRESUMEN
In a prospective study, the toxicity and efficacy of an instillation therapy with recombinant interferon alpha-2a (rIFN-alpha-2a) were evaluated in 12 patients with superficial bladder tumors. Treatment consisted of 8 weekly instillations of 54 X 10(6) IU rIFN-alpha-2a in 50 ml saline. Two weeks after completion of the instillation therapy, the tumor status was assessed with cystoscopy, biopsy and bladder wash-out cytology. Two partial responses, 1 no change and 2 progressive disease were seen in the 5 patients with TA tumors. In the 4 patients with carcinoma in situ, 1 complete response, 1 partial response and 2 no change were observed. Three patients suffered from carcinoma in situ and superficial papillary tumors, 1 showed complete response of the carcinoma in situ but no change of the TA tumor, the other 2 patients showed progressive disease. Three patients with partial response received a follow-up combination therapy with interferon intravesically and etretinate orally (25 mg/day). These patients presented progressive disease or no change 10 weeks after starting the follow-up combination therapy. During the treatment period, no side effects of interferon or changes of the serum interferon levels were observed. The treatment results are considered unsatisfactory; nevertheless, some activity after intravesical administration of interferon (mainly in patients with carcinoma in situ) could be demonstrated. Since the cytotoxic and antiproliferative effects seem to be dose-dependent, further studies might be done using higher interferon dosages and shorter treatment intervals.
Asunto(s)
Carcinoma in Situ/terapia , Carcinoma Papilar/terapia , Interferón Tipo I/uso terapéutico , Proteínas Recombinantes/uso terapéutico , Neoplasias de la Vejiga Urinaria/terapia , Administración Intravesical , Adulto , Anciano , Ensayos Clínicos como Asunto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estudios ProspectivosRESUMEN
The results of treatment of metastatic renal cell carcinoma have so far been very poor. Many phase II studies have shown that interferon alpha therapy is active in a significant proportion of patients (approximately 10 to 15% complete and partial remission). In the hope of improving these results we have conducted a phase I-II study of the combination of interferon alpha-2a and vinblastine in 21 patients with metastatic renal cell cancer. Side-effects were pronounced and the mean tolerated doses were 12.5 x 10(6) U/m2 interferon alpha three times per week and 0.10 mg/kg vinblastine once every three weeks. We observed a 43% response rate, with 1 complete remission, 8 partial remissions, 4 stabilizations and 8 progressions. These very encouraging results need to be confirmed by large scale studies.
Asunto(s)
Adenocarcinoma/tratamiento farmacológico , Interferón Tipo I/uso terapéutico , Neoplasias Renales/tratamiento farmacológico , Vinblastina/uso terapéutico , Adenocarcinoma/terapia , Adulto , Anciano , Evaluación de Medicamentos , Quimioterapia Combinada , Femenino , Humanos , Neoplasias Renales/terapia , Masculino , Persona de Mediana Edad , Metástasis de la Neoplasia , Proteínas Recombinantes/uso terapéutico , Factores de TiempoRESUMEN
Usual treatments of metastatic renal cell carcinoma are not efficient. Phase II trials using Interferon alpha showed a low response rate (10 to 15%). We have conducted a phase I-II trial using a combination of Vinblastine and Recombinant alpha 2 A Interferon in 21 patients. The response rate is of 43% including 1 complete and 8 partial remissions, 5 stabilizations and 8 progressive diseases. In spite of important side effects, these results are promising.
Asunto(s)
Carcinoma de Células Renales/terapia , Interferón Tipo I/uso terapéutico , Neoplasias Renales/terapia , Proteínas Recombinantes/uso terapéutico , Vinblastina/uso terapéutico , Adulto , Anciano , Carcinoma de Células Renales/tratamiento farmacológico , Carcinoma de Células Renales/secundario , Evaluación de Medicamentos , Quimioterapia Combinada , Femenino , Estudios de Seguimiento , Humanos , Interferón Tipo I/efectos adversos , Neoplasias Renales/tratamiento farmacológico , Masculino , Persona de Mediana Edad , Vinblastina/efectos adversosRESUMEN
The infection of the edges of a hysterotomy wound by anaerobic bacteria is a very rare and dangerous complication of Caesarean section. Early surgical intervention with trimming of the edges and the institution of drainage together with suitable antibiotic treatment led to recovery. A second pregnancy, which unfortunately ended with a premature delivery, resulted after this conservative surgical procedure.
Asunto(s)
Infecciones Bacterianas/etiología , Infección de la Herida Quirúrgica/terapia , Adulto , Bacterias Anaerobias , Infecciones Bacterianas/terapia , Cesárea/métodos , Femenino , Humanos , Necrosis , Infección de la Herida Quirúrgica/patología , Útero/patologíaRESUMEN
Routine obstetrical ultrasound carried out in the 20th week of amenorrhoea made it possible to identify a septate abdominal cyst which was non-urinary in origin and situated in the right hypochondrium. It was difficult, armed with this information, to be sure from which organ the cyst derived. The secondary diagnosis of a congenital cyst of the bile duct was made at laparotomy carried out a few hours after birth.