RESUMEN
O óleo essencial de Rosmarinus officinalis é utilizado em perfumes e cosméticos, na indústria alimentícia e em medicamentos, apresentando também atividade antimicrobiana. Há informações de que o óleo essencial poderia estar envolvido com alterações de membrana celular e que outros extratos de R. officinalis poderiam interferir com síntese/secreção de hormônios esteroidianos, concentração de glicose e proliferação de células endoteliais, processos que poderiam alterar o desenvolvimento embrio-fetal, o que levou a se planejar o presente trabalho com o objetivo de verificar o desenvolvimento fetal em ratas tratadas com R. officinalis. Ratas Wistar do Biotério do Centro de Biologia da Reprodução foram inseminadas por machos férteis comprovada e distribuídas aleatoriamente em grupos controle e tratados 1, 2 e 3 (T1, T2 e T3) que receberam, respectivamente: 242 mg/ kg; 484 mg/ kg e 968 mg/ kg de óleo essencial de R. officinalis. Os animais foram tratados do 80 ao 150 dia pós-coito e submetidos a eutanásia por excesso de anestesia no 210 dia. Variáveis observadas: indícios clínicos, hematológicos e bioquímicos de toxicidade materna e parâmetros reprodutivos, peso corporal e de órgãos maternos e fetais. Não foram observadas diferenças significativas nas variáveis maternas analisadas, havendo apenas aumento da concentração de uréia que não foi esclarecido. O peso corporal e o de cérebr
RESUMEN
The Jarsin extract (Hypericum perforatum L., 1753), is used as an alternative of the synthetic antidepressants. The are few paper concerning the reproductive toxicology of Jarsin extract and none related to the early embryonic development. The present work was aimed at verifying the effect of Jarsin extract, on the blastocyst development in Wistar rats. Female rats from the Centro de Biologia da Reprodução vivarium were mated with fertile males. After the insemination, the femalerats were randomly distributed in two groups: Control (0.5 ml ofdistilled water) and treated (18mg of H. perforatum/0.5 ml of distilled water). Both groups received the treatment by oral gavage, twice a day from the first to the fourth day of gestation. The animals were terminated on the fifth day of gestation. The oviduct and uterine cornua were flushedwith Talpe-Hepes medium and the embryos were collected, counting and their morphologic development performed. The dams were evaluated to clinical signs indicative of maternal intoxication (deaths, altered locomotion in the cage, piloerection, diarhoea, vaginal bleeding, reducedfood consumption and body weight gain or loss). Maternal ovary weight was measured and the number of corpora lutea was counted. The data were analyzed by the Students t test (a = 0,05). No clinical indicative of maternal intoxication were observed. The number of embryos and
RESUMEN
The Jarsin extract (Hypericum perforatum L., 1753), is used as an alternative of the synthetic antidepressants. The are few paper concerning the reproductive toxicology of Jarsin extract and none related to the early embryonic development. The present work was aimed at verifying the effect of Jarsin extract, on the blastocyst development in Wistar rats. Female rats from the Centro de Biologia da Reprodução vivarium were mated with fertile males. After the insemination, the femalerats were randomly distributed in two groups: Control (0.5 ml ofdistilled water) and treated (18mg of H. perforatum/0.5 ml of distilled water). Both groups received the treatment by oral gavage, twice a day from the first to the fourth day of gestation. The animals were terminated on the fifth day of gestation. The oviduct and uterine cornua were flushedwith Talpe-Hepes medium and the embryos were collected, counting and their morphologic development performed. The dams were evaluated to clinical signs indicative of maternal intoxication (deaths, altered locomotion in the cage, piloerection, diarhoea, vaginal bleeding, reducedfood consumption and body weight gain or loss). Maternal ovary weight was measured and the number of corpora lutea was counted. The data were analyzed by the Students t test (a = 0,05). No clinical indicative of maternal intoxication were observed. The number of embryos and