A new experimental model of cigarette smoke-induced emphysema in Wistar rats.

OBJECTIVE: To describe a new murine model of cigarette smoke-induced emphysema. METHODS: Twenty-four male Wistar rats were divided into two groups: the cigarette smoke group, comprising 12 rats exposed to smoke from 12 commercial filter cigarettes three times a day (a total of 36 cigarettes per day) every day for 30 weeks; and the control group, comprising 12 rats exposed to room air three times a day every day for 30 weeks. Lung function was assessed by mechanical ventilation, and emphysema was morphometrically assessed by measurement of the mean linear intercept (Lm). RESULTS: The mean weight gain was significantly (approximately ten times) lower in the cigarette smoke group than in the control group. The Lm was 25.0% higher in the cigarette smoke group. There was a trend toward worsening of lung function parameters in the cigarette smoke group. CONCLUSIONS: The new murine model of cigarette smoke-induced emphysema and the methodology employed in the present study are effective and reproducible, representing a promising and economically viable option for use in studies investigating the pathophysiology of and therapeutic approaches to COPD.

A new experimental model of cigarette smoke-induced emphysema in Wistar rats / Um novo modelo experimental murino de enfisema: enfisema induzido pela fumaça do cigarro em ratos Wistar

OBJECTIVE: To describe a new murine model of cigarette smoke-induced emphysema. METHODS: Twenty-four male Wistar rats were divided into two groups: the cigarette smoke group, comprising 12 rats exposed to smoke from 12 commercial filter cigarettes three times a day (a total of 36 cigarettes per day) every day for 30 weeks; and the control group, comprising 12 rats exposed to room air three times a day every day for 30 weeks. Lung function was assessed by mechanical ventilation, and emphysema was morphometrically assessed by measurement of the mean linear intercept (Lm). RESULTS: The mean weight gain was significantly (approximately ten times) lower in the cigarette smoke group than in the control group. The Lm was 25.0% higher in the cigarette smoke group. There was a trend toward worsening of lung function parameters in the cigarette smoke group. CONCLUSIONS: The new murine model of cigarette smoke-induced emphysema and the methodology employed in the present study are effective and reproducible, representing a promising and economically viable option for use in studies investigating the pathophysiology of and therapeutic approaches to COPD. .

OBJETIVO: Descrever um novo modelo murino de enfisema induzido pela fumaça de cigarro. MÉTODOS: Vinte e quatro ratos Wistar foram divididos em dois grupos: o grupo fumaça de cigarro, com 12 ratos expostos à fumaça de 12 cigarros comerciais com filtro três vezes ao dia (um total de 36 cigarros por dia), sete dias por semana, durante 30 semanas e o grupo controle, com 12 animais expostos ao ar ambiente três vezes ao dia, sete dias por semana, durante 30 semanas. A função pulmonar foi avaliada por meio de ventilação mecânica, e o enfisema foi morfometricamente avaliado por meio do diâmetro alveolar médio (Lm). RESULTADOS: A média de ganho de peso foi significativamente menor (aproximadamente dez vezes menor) no grupo fumaça de cigarro do que no grupo controle. O Lm foi 25.0% maior no grupo fumaça de cigarro. Os parâmetros de função pulmonar tenderam a ser piores no grupo fumaça de cigarro. CONCLUSÕES: O novo modelo murino de enfisema induzido pela fumaça de cigarro e a metodologia empregada neste estudo são eficazes e reproduzíveis; são, portanto, uma opção promissora e economicamente viável para estudos sobre a fisiopatologia e o tratamento da DPOC. .

Cell therapy with bone marrow mononuclear cells in elastase-induced pulmonary emphysema.

Emphysema is characterized by destruction of alveolar walls with loss of gas exchange surface and consequent progressive dyspnea. This study aimed to evaluate the efficiency of cell therapy with bone marrow mononuclear cells (BMMC) in an animal model of elastase-induced pulmonary emphysema. Emphysema was induced in C57Bl/J6 female mice by intranasal instillation of elastase. After 21 days, the mice received bone marrow mononuclear cells from EGFP male mice with C57Bl/J6 background. The groups were assessed by comparison and statistically significant differences (p < 0.05) were observed among the groups treated with BMMC and evaluated after 7, 14 and 21 days. Analysis of the mean linear intercept (Lm) values for the different groups allowed to observe that the group treated with BMMC and evaluated after 21 days showed the most significant result. The group that received no treatment showed a statistically significant difference when compared to other groups, except the group treated and evaluated after 21 days, evidencing the efficacy of cell therapy with BMMC in pulmonary emphysema.