RESUMEN
We performed an electron microscopic study of the small intestine of suckling rabbits infected with cholerogenic and non-cholerogenic strains nonO1/nonO139 Vibrio cholerae. Cholerogenic strain induced mostly hydropic degeneration of the epithelium typical of cholera toxin effect, while non-cholerogenic strain induced the formation of lacunae along the borders of adjacent epithelial cells typical of hemagglutinin/protease effect. In both cases, reduction of microvilli, destruction of intracellular organelles, two types of mitochondrial reaction (condensation and swelling with destruction of cristae), appearance of myelin figures, defects in the capillary walls, and activation of pinocytosis were observed. These data confirm our previous assumption on interchangeability of different pathogenic factors of Vibrio cholerae, including nonO1/nonO139 strains.
Asunto(s)
Cólera/patología , Interacciones Huésped-Patógeno , Intestino Delgado/ultraestructura , Mitocondrias/ultraestructura , Vibrio cholerae/patogenicidad , Factores de Virulencia/metabolismo , Animales , Animales Lactantes , Cólera/microbiología , Toxina del Cólera/metabolismo , Intestino Delgado/microbiología , Intestino Delgado/patología , Metaloendopeptidasas/metabolismo , Microscopía Electrónica , Mitocondrias/microbiología , Mitocondrias/patología , Pinocitosis , Conejos , Especificidad de la Especie , Vibrio cholerae/fisiologíaRESUMEN
The development of experimental cholera in suckling rabbits is associated with typical cholerogenic syndrome: the presence of Vibrio cholerae in the blood, bile (in 60 and 70% cases, respectively), small and large intestine (in 100% cases). Simultaneously with enterocyte desquamation and increased permeability of the blood-enterocyte barrier, the vibrios are released into villous stroma and then into the microcirculatory bed. the zot toxin is involved in the mechanism of Vibrio cholerae invasion, the corresponding gene is present in the genome of the studied strain.
Asunto(s)
Cólera/metabolismo , Intestino Delgado , Vibrio cholerae/metabolismo , Animales , Animales Recién Nacidos , Animales Lactantes , Cólera/microbiología , Toxina del Cólera/metabolismo , Endotoxinas , Eritrocitos/microbiología , Eritrocitos/ultraestructura , Intestino Delgado/metabolismo , Intestino Delgado/patología , Intestino Delgado/ultraestructura , Conejos , Vibrio cholerae/ultraestructuraRESUMEN
Ultrastructural analysis of the jejunum in suckling rabbits showed that lipofibroblasts localized in the submucosa and adjacent to crypts contain lipid inclusions (granules) with typical "melting" surface. Lipofibroblasts contained moderately widened cisternae of the granular endoplasmic reticulum and few mitochondria with dense matrix and poorly developed cristae. Experimental cholera was usually accompanied by a decrease in the number of lipid inclusions, and only in some cases by accumulation of lipid material. Our results suggest that the material accumulated in granules plays a role in the pathogenesis of cholera.
Asunto(s)
Cólera/patología , Cuerpos de Inclusión/ultraestructura , Yeyuno/patología , Metabolismo de los Lípidos , Animales , Animales Lactantes , Fibroblastos/ultraestructura , Yeyuno/ultraestructura , ConejosRESUMEN
Ultrastructural study of the small intestine in suckling rabbits with experimental cholera revealed involvement of the inner and outer smooth muscle layers into the pathological process. Smooth muscle cells were characterized by vacuolar and fatty degeneration and focal colliquative necrosis. Apoptosis played little role in gastrointestinal motility disturbances. The presence of considerable amounts of fluid in intestinal loops reflects peristaltic dysfunction due to generalized damage to smooth muscle cells.
Asunto(s)
Cólera/patología , Intestino Delgado/patología , Miocitos del Músculo Liso/ultraestructura , Vibrio cholerae/metabolismo , Animales , Apoptosis , Núcleo Celular/patología , Modelos Animales de Enfermedad , Intestino Delgado/ultraestructura , Microscopía Electrónica , Miocitos del Músculo Liso/microbiología , Miocitos del Músculo Liso/patología , Necrosis , Conejos , Factores de TiempoRESUMEN
Intranuclear inclusions detected in hepatic, renal, and cerebral cells during endotoxemia can be subdivided into true and pseudo inclusions. True inclusions have filamentous and crystalline structure and are rarely found in control animals. However, the number of true inclusions increases during pathology and can serve as a marker of molecular pathology in the cells provoked by endotoxin.
Asunto(s)
Endotoxemia , Endotoxinas , Animales , Núcleo Celular/ultraestructura , Perros , Endotoxinas/farmacología , Células Epiteliales/metabolismo , Hepatocitos/ultraestructura , Riñón/ultraestructura , Túbulos Renales/ultraestructura , Hígado/ultraestructura , Masculino , Corteza Motora/ultraestructura , Neuronas/metabolismo , Conejos , RatasRESUMEN
Immature nephrons and the presence of a proteolytic system mediating extracellular protein digestion in the epithelium of proximal tubules are characteristic features of renal cortical ultrastructure in intact 10-12-day-old suckling rabbits. Cholera infection is accompanied by intensive cleavage of exogenous protein, which starts in podocytes and is completed in the cytoplasm of the proximal tubule epitheliocytes. Overload to nephron associated (in addition to urine production) with intensive extracellular hydrolysis impairs nephrothelium reactivity and increases its vulnerability to cholera exo- and endotoxins and their mediators.