[Prospects of use coryneformic bacteria drug to prevent and treat respiratory infections].

This work shows the Codivac efficiency in its aerosol form to treat children sick of influenza and acute respiratory viral infections. Course of therapy with the Codivac drug leads to improve clinical picture and rapid elimination virus from human organism as compared with children undergone traditional therapy. Recovery is accompanied with improving of indices of cell immunity. The article shows the prospects of use Codivac to prevent and treat respiratory infections.

[The humoral immunity against diphtheria].

Results of the conducted study showed that naturally acquired antibacterial and postvaccinal antitoxic antibodies against diphtheria were found in human blood sera. Challenge of ADT-M toxoid to adults resulted in production of antitoxic as well as antibacterial antibodies in high concentrations. In response to challenge of ADT-M toxoid simultaneously with bacterial vaccine against diphtheria Codivac both antibacterial and antitoxic antibodies were synthesized in blood on optimal physiologic levels. This study revealed dynamics of some specific characteristics of humoral immune response after challenge of two different vaccines against diphtheria--ADT-M toxoid and Codivac vaccine.

[Immunoloical aspects of the antropogenic response to the antigens of symbiotic non-toxigenic diphtherial corynebacteria].

Corynebacteria, being ancient symbionts of open cavities of human body, carry unique, balanced immunogenic stimuli, formed in the process of evolution, thus maintaining non-specific resistance at a certain level. They favor formation of human microcenotic communities as a normal biological and physiological phenomenon. Codivak, a preparation of natural antigens of a symbiotic strain of non-toxigenic diphtherial corynebacteria, is able to correct not only disturbances of oropharyngeal immunity, but also general cell-mediated and humoral immunity disorders.

[Specific antibodies and their role in the formation of antidiphtheria immunity].

In human sera, studied with the use of the enzyme immunoassay, antidiphtheria postvaccinal antitoxic IgG and naturally acquired antibacterial IgG, IgM and IgA were detected. In the blood of children and adults aged up to 50 years antitoxic IgG were present at normal and high concentrations. In 50% of children antibacterial IgA were absent, while specific antibacterial IgM could be found at high concentrations. Changes in the content of antibacterial antibodies of different classes in sera were observed with age. More than 90% of adults had antibacterial IgA and IgG at normal and hig concentrations, while the level of IgM decreased. Under the influence of ecological, social, anthropogenic and other environmental factors the optimum levels of specific antibodies were replaced by anomalous ones, which led to an increased number of persons susceptible to diphtheria infection and in the intensity of the circulation of the infective agent. The deficiency of antidiphtheria antibacterial antibodies in the blood determined the necessity of correcting immunity by means of not only toxoid, but also bacterial antigens.

[A method for the primary selection of immunomodulators by the level of 5'-nucleotidase activity in the macrophages of peritoneal exudate]. / Sposob pervichnogo otbora immunomoduliatorov po urovniu aktivnosti 5'-nukleotidazy makrofagov peritoneal'nogo ékssudata.

A method of primary selection of immunomodulators based on measuring of ecto 5'-nucleotidase activity level of macrophages of peritoneal exudate was developed. Immunostimulators were found to decrease the activity level, while immunosuppressors increased the activity. The preparations having no immunomodulatory effect do not change the activity of this enzyme.

[Effect of T-activin on macrophage 5'-nucleotidase activity and blood cortisol level depending on the time of day]. / Osobennosti vliianiia T-aktivina na aktivnost' 5-nukleotidazy makrofagov i uroven' kortizola krovi v zavisimosti ot vremeni sutok.

Peculiarities of T-activin influence on macrophage 5-nucleotidase and blood cortisol in dependence of day time have been investigated on CBA and C57/B16 mice. It has been established that T-activin influence on already studied indexes in broad range of doses; from 10 to 1 mkg for 1 mouse.

[Effect of plague microbe fraction 1 protein-polysaccharide complex and pure protein on metabolic parameters of macrophages]. / Vliianie belkovopolisakharidnogo kompleksa i chistogo belka fraktsii 1 chumnogo mikroba na metabolicheskie pokazateli makrofagov.

The investigation reveals different influence of the plague microbe's fraction 1 polysaccharide-protein complex and of it's purified protein on the 5'-nucleotidase activity and on the chemiluminescence response of peritoneal macrophages. Both of these metabolic indexes were found to be dependent on the dose of fraction 1 and duration of time till examination.

[Analysis of the immunomodulating action of SK in mice of various strains]. / Analiz immunomoduliruiushchego deistviia SK u mushei raznykh linii.

Peculiarities of immunomodulative action of different mice breeds' SK have been determined by changes of activity level of 5'-nucleotidase in macrophages from peritoneal exudate (MPE) and by changes of animals' sensitivity to staphylococcus infection. It has been established that the character of SK influence on activity level of 5'-nucleotidase in MPE and resistance depends on dose, type of administration and the mice breed. Revealed by hypodermic injection, dependence between the activity of 5'-nucleotidase in MPE and sensitivity to staphylococcus infection, whose character was determined by animals' genotype, had straight character in C57Bl/6 mice, and reverse character in CBA mice.

[Effect of immobilization stress on macrophage 5'-nucleotidase activity and endogenous cortisol level in mice of the CBA and C57Bl/6 strains]. / Osobennosti vliianiia immobilizatsionnogo stressa na aktivnost' 5'-nukleotidazy makrofagov i uroven' éndogennogo kortizola u myshei linii SBA i C57Bl/6.

Complex research of peculiarities of immobilization stress influence on activity of 5'-nucleotidase from macrophages, got from peritoneal exudate and level of endogenous cortisol in blood of CBA and C57Bl/6 mice has been carried out. Genotype peculiarities of macrophage and endocrine index reaction on stress influence have been found.

[Effect of immunomodulators of organometallic nature on the sensitivity to plague toxin]. / Vliianie immunomoduliatorov metallorganicheskoi prirody na chuvstvitel'nost' k chumnomu toksinu.

Influence of immunomodulators with organometallic origin on sensitivity to mice toxin on CBA, C Bl/6 mice and hybrids F1 (CBA C Bl/6) has been investigated. It has been determined that used immunomodulators are the means of urgent change of sensitivity to plague intoxication (5 min after i.v. administration of immunomodulators, the sensitivity to mice toxin changes). The character of immunomodulators' influence on sensitivity to plague intoxication depends on daily time and animals' genotype.

[Characteristics of immunomodulating effect of histamine in inbred strain mice]. / Osobennosti immunomoduliruiushchego deistviia gistamina u myshei inbrednykh linii.

The activity of 5'-nucleotidase of peritoneal exudate in subcutaneous injection of histamine in a dose of 1.0-100 microliters was studied in mice of different lines (CBA, C57, B1/6, Balb/c, NFS/n, NFR/n). There were interline differences in the influence of histamine on this metabolic index.

[Immunomodulating properties of the antigens of the cell walls of nontoxigenic Corynebacterium diphtheriae]. / Immunomoduliruiushchie svoistva antigenov kletochnykh stenok netoksigennykh korinebakterii difterii.

The total protein fraction was obtained by extraction with nonionic detergent NP-40. The fraction composition and molecular parameters were studied with DDS-Na electrophoresis in polyacryl amide gel. Common properties of the Corynebacterium diphtheriae protein fraction 66 kD and an analogous fraction of the cell walls of staphylococci and the measles virus were shown with immunoblotting. Cell-mediated mechanisms of the immune response to contact with antigens of Corynebacterium diphtheriae cell walls were revealed.