RESUMEN
We report here the first complete transcriptome analysis of the dorsal (dDG) and ventral dentate gyrus (vDG) of a rat epilepsy model presenting a hippocampal lesion with a strict resemblance to classical hippocampal sclerosis (HS). We collected the dDG and vDG by laser microdissection 15 days after electrical stimulation and performed high-throughput RNA-sequencing. There were many differentially regulated genes, some of which were specific to either of the two sub-regions in stimulated animals. Gene ontology analysis indicated an enrichment of inflammation-related processes in both sub-regions and of axonal guidance and calcium signaling processes exclusively in the vDG. There was also a differential regulation of genes encoding molecules involved in synaptic function, neural electrical activity and neuropeptides in stimulated rats. The data presented here suggests, in the time point analyzed, a remarkable interaction among several molecular components which takes place in the damaged hippocampi. Furthermore, even though similar mechanisms may function in different regions of the DG, the molecular components involved seem to be region specific.
Asunto(s)
Giro Dentado/metabolismo , Epilepsia/metabolismo , Regulación de la Expresión Génica , Secuenciación de Nucleótidos de Alto Rendimiento , Esclerosis Tuberosa/metabolismo , Animales , Giro Dentado/patología , Epilepsia/patología , Masculino , Ratas , Ratas Wistar , Esclerosis Tuberosa/patologíaRESUMEN
Sixteen symptom-free siblings of children with vesicoureteral reflux were found to have the condition and underwent renal scintigraphy with technetium 99m dimercaptosuccinic acid. Of these 16 children, 6 had scintigraphic evidence of renal damage. Screening for vesicoureteral reflux in siblings of children known to have the condition should therefore be carried out early in life; when reflux is present, an immediate assessment of the renal parenchyma should be made.
Asunto(s)
Enfermedades Renales/diagnóstico por imagen , Riñón/diagnóstico por imagen , Compuestos de Organotecnecio , Succímero , Reflujo Vesicoureteral/complicaciones , Niño , Preescolar , Femenino , Humanos , Lactante , Enfermedades Renales/etiología , Masculino , Cintigrafía , Ácido Dimercaptosuccínico de Tecnecio Tc 99m , Reflujo Vesicoureteral/genéticaAsunto(s)
Cálculos Renales/etiología , Errores Innatos del Metabolismo de la Purina-Pirimidina/genética , Xantina Oxidasa/deficiencia , Xantinas/orina , Genes Recesivos , Humanos , Lactante , Masculino , Errores Innatos del Metabolismo de la Purina-Pirimidina/complicaciones , Errores Innatos del Metabolismo de la Purina-Pirimidina/orina , XantinaRESUMEN
Two hundred twenty-three children who underwent anti-reflux surgery were evaluated. Pyelonephritic scarring was more apt to occur with greater degrees of reflux. Postoperative infection was principally confined to the bladder and occurred primarily in females. The beneficial effect of anti-reflux surgery is discussed.