RESUMEN
A total of 234 M. tuberculosis isolates were used to demonstrate the leading role of mutations in, respectively, codon 531 of gene rpoB (90.0%) and codon 315 of gene katG (92.9%), in the development of resistance to rifampicin and isoniazid by the methods of reverse hybridization with oligonucleotide probes and the sequencing of gene stretches. The levels of primary resistance of M. tuberculosis to rifampicin, isoniazid and multiresistance, according to the molecular-genetic analysis, were 41.0%, 57.7% and 37.2% respectively. The coincidence of the results of the bacteriological and molecular-genetic analyses of the antimicrobial resistance of the isolates was 90.4% and 95.3% for isoniazid and rifampicin respectively. The prevalence of individual types of mutations, linked with antimicrobial resistance, in the presence of a considerable spread of strains of the family Beijing in the region may be indicative of the limited number of M. tuberculosis clones circulating in the region.
Asunto(s)
Farmacorresistencia Bacteriana Múltiple/genética , Mycobacterium tuberculosis/genética , Antibióticos Antituberculosos/farmacología , Proteínas Bacterianas/genética , Catalasa/genética , Codón , ARN Polimerasas Dirigidas por ADN/genética , Humanos , Isoniazida/farmacología , Mutación , Mycobacterium tuberculosis/efectos de los fármacos , Mycobacterium tuberculosis/aislamiento & purificación , Sondas de Oligonucleótidos , Rifampin/farmacología , Federación de Rusia , Tuberculosis/microbiologíaRESUMEN
Results of molecular-genetic analysis of Mycobacterium tuberculosis strains isolated from patients of Odessa and Nikolaev regions of Ukraine are represented. Occurrence of individual types of mutations in genes associated with rifampicin and isoniazid resistance has been studied for the first time. Data concerning prevalence of Beijing strains in the South of Ukraine are obtained.
Asunto(s)
Proteínas Bacterianas/genética , Catalasa/genética , ADN Bacteriano/análisis , Mycobacterium tuberculosis/genética , Oxidorreductasas/genética , Tuberculosis Resistente a Múltiples Medicamentos/microbiología , Antituberculosos/uso terapéutico , Codón/genética , Dermatoglifia del ADN , Farmacorresistencia Bacteriana Múltiple/genética , Humanos , Mutación , Mycobacterium tuberculosis/aislamiento & purificación , Reacción en Cadena de la Polimerasa , Tuberculosis Resistente a Múltiples Medicamentos/tratamiento farmacológico , Tuberculosis Resistente a Múltiples Medicamentos/epidemiología , Ucrania/epidemiologíaAsunto(s)
Sistema Inmunológico/fisiología , Deportes/fisiología , Adolescente , Adulto , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Activity of peroxidase (AP) and maintenance of lysosomal cation proteins (CP) were studied during phagocytic reaction of polymorphonuclear leukocytes of healthy persons of different age and those with acute respiratory disease and chronic tonsillitis. It is found that during acute virus and chronic bacterial infection similar changes in AP and CP dynamics are observed, which is correlated with a decrease of phagocytic digesting function.
Asunto(s)
Infecciones Bacterianas/sangre , Proteínas Sanguíneas/metabolismo , Neutrófilos/metabolismo , Peroxidasas/sangre , Fagocitosis/inmunología , Neumonía Viral/sangre , Tonsilitis/sangre , Adulto , Factores de Edad , Infecciones Bacterianas/inmunología , Preescolar , Humanos , Técnicas In Vitro , Lactante , Neutrófilos/enzimología , Neutrófilos/inmunología , Peroxidasas/deficiencia , Neumonía Viral/inmunología , Tonsilitis/inmunologíaRESUMEN
Study of the qualitative and quantitative indicators of reprint smears from the surface of the upper respiratory mucosa in healthy infants and in these with acute respiratory viral infection has shown that migrating polymorphonuclear leukocytes take an active part in the functioning of the barrier of the upper respiratory mucosa at the early stages of human ontogenesis. The cytogram and cytochemical characterization of peprint smear cells from the upper respiratory mucosa in infants afflicted with acute respiratory viral infection are sensitive tests in the assessment of defence potentialities of the child's body.
