RESUMEN
BACKGROUND: Skin sensitivity is a common problem in the Western population correlated with changes of skin properties like skin barrier function, hydration and skin physiology. Skin properties can be modulated by dietary fatty acids (FA), especially poly-unsaturated FA. The present study was performed to evaluate the effect of daily supplementation with flaxseed oil and safflowerseed oil on healthy volunteers with sensitive skin. METHODS: The study was designed as a randomized, double-blind 12-week intervention with 2 female treatment groups (n = 13). Plasma FA profile, skin sensitivity, skin hydration, transepidermal water loss (TEWL) and skin surface were evaluated on day 0, week 6 and week 12. RESULTS: Supplementation with flaxseed oil led to significant decreases in sensitivity (after nicotinate irritation), TEWL, skin roughness and scaling, while smoothness and hydration were increased. Concomitantly, the ratio of n-6/n-3 FA in plasma decreased. Upon supplementation with safflowerseed oil, only a significant improvement in skin roughness and hydration was observed; however, the effects were less pronounced and determined at a later point in time than with flaxseed oil. The plasma n-6/n-3 FA ratio increased. CONCLUSION: The data provide evidence that daily intake of flaxseed oil modulates skin condition.
Asunto(s)
Suplementos Dietéticos , Eritema/prevención & control , Aceite de Linaza/administración & dosificación , Aceite de Cártamo/administración & dosificación , Piel/efectos de los fármacos , Pérdida Insensible de Agua/efectos de los fármacos , Administración Oral , Cápsulas , Método Doble Ciego , Eritema/diagnóstico , Eritema/metabolismo , Eritema/patología , Ácidos Grasos Omega-3/sangre , Ácidos Grasos Omega-6/sangre , Femenino , Humanos , Flujometría por Láser-Doppler , Aceite de Linaza/metabolismo , Microcirculación , Ácidos Nicotínicos , Flujo Sanguíneo Regional , Aceite de Cártamo/metabolismo , Piel/irrigación sanguínea , Piel/metabolismo , Piel/patología , Pruebas de Irritación de la Piel , Factores de Tiempo , Resultado del TratamientoRESUMEN
In the present study we investigated the influence of two different antioxidant supplements composed of carotenoids, vitamin E and selenium on parameters related to skin health and skin aging. Thirty-nine volunteers with healthy, normal skin of skin type 2 were divided into 3 groups (n = 13) and supplemented for a period of 12 weeks. Group 1 received a mixture of lycopene (3 mg/day), lutein (3 mg/day), beta-carotene (4.8 mg/day), alpha-tocopherol (10 mg/day) and selenium (75 microg/day). Group 2 was supplemented with a mixture of lycopene (6 mg/day), beta-carotene (4.8 mg/day), alpha-tocopherol (10 mg/day) and selenium (75 microg/day). Group 3 was the placebo control. Upon supplementation serum levels of selected carotenoids increased in both verum groups. Skin density and thickness were determined by ultrasound measurements. A significant increase for both parameters was determined in the verum groups. Roughness, scaling, smoothness and wrinkling of the skin were determined by Surface Evaluation of Living Skin (Visioscan). Roughness and scaling were improved by the supplementation with antioxidant micronutrients. In the placebo group no changes were found for any of the parameters.
Asunto(s)
Antioxidantes/análisis , Antioxidantes/farmacología , Suplementos Dietéticos , Envejecimiento de la Piel/efectos de los fármacos , Piel/efectos de los fármacos , Adolescente , Adulto , Carotenoides/sangre , Carotenoides/farmacología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Selenio/sangre , Selenio/farmacología , Piel/anatomía & histología , Piel/diagnóstico por imagen , Fenómenos Fisiológicos de la Piel/efectos de los fármacos , Ultrasonografía , Vitamina E/sangre , Vitamina E/farmacologíaRESUMEN
BACKGROUND/AIMS: The ultraviolet (UV) portion of sunlight is involved in the induction and development of skin cancers against which a limited photoprotection may be provided by reduced time of exposure, clothing, and sunscreen applications. The concept of an effective, safe, systemic photoprotection will circumvent many of the shortcomings. The UV-induced oxidative stress is a cause of DNA damage and a few publications have shown, in humans, minimal benefits, if any, of the oral intake of antioxidant complex, contrasting with the large literature showing beneficial effects in vitro or in animal models. METHODS: We investigated, in 25 healthy individuals, the capacity of an antioxidant complex (AOC) - vitamins (lycopene, beta-carotene, alpha-tocopherol), selenium - to reduce UV-induced damages. The AOC was administered orally, daily during 7 weeks. Before and after irradiations, before and after the intake of the product, six parameters were studied: skin color by chromametry, minimal erythemal dose and, on skin biopsies, sunburn cells (SBCs), p53 detected by immunohistochemistry, pigmentation index, and levels of lipoperoxides (thiobarbituric acid reaction). RESULTS: After the oral intake of AOC, we observed an elevation of the actinic erythema threshold (+20%, P=0.01) and a general reduction of the UV-induced erythemas, a reduction of the UV-induced p53 expression (P<0.05) and of SBCs (P<0.01), and a parallel reduction of the lipoperoxide levels (P<0.01). The pigmentation was increased (P<0.01). CONCLUSION: After the oral intake of an antioxidant complex, many parameters of the epidermal defense against UV-induced damages are significantly improved. The oral intake of AOC could provide a safe, daylong and efficient complement to photo-protective measures provided by topical and physical agents and may contribute to reduce the DNA damages leading to skin aging and skin cancers.
Asunto(s)
Antioxidantes/farmacología , Carotenoides/farmacología , Piel/efectos de los fármacos , Quemadura Solar/prevención & control , Rayos Ultravioleta/efectos adversos , Administración Oral , Adulto , Análisis de Varianza , Antioxidantes/administración & dosificación , Carotenoides/administración & dosificación , Quimioterapia Combinada , Femenino , Humanos , Técnicas para Inmunoenzimas , Licopeno , Masculino , Selenio/farmacología , Envejecimiento de la Piel/efectos de los fármacos , Resultado del TratamientoRESUMEN
The aim of this study was to demonstrate that modification of the cellular redox-equilibrium occurs as a consequence of antioxidant nutrients intake (carotenoids, vitamine E and vitamine C) and that these nutrients play a role in the pigmentation of the skin without any UV exposure. We conducted a randomized, double-blind study in 20 healthy subjects to evaluate and to compare the efficacy of two mixtures of dietary antioxidants with regard to direct determination of melanin and carotenes by chromametry at selected skin sites and multiple reflection spectrometry from a 1 cm2 region of skin of different parts of the body. Efficacy was assessed by a significant improvement of these parameters, in comparison with measurements performed on the day of randomization, before dietary supplement intake. The formulations per capsule of study dietary supplements are: 13 mg of beta-carotene, 2 mg of lycopene, 5 mg of vitamine E and 30 mg of vitamine C (B13/L2) or 3 mg of beta-carotene, 3 mg of lycopene, 5 mg of vitamine E and 30 mg of vitamine C (B3/L3). A 8-week B13/L2-supplementation lead to a detectable carotenodermia whereas the B3/L3-supplementation not. Signicative increase of melanin concentrations in skin were found after 4, 5, 6 and 8 weeks of dietary antioxidant intake in both groups (p < 0.05). These results are discussed with regard to the redox control theory of melanocytes which regulates the tyrosinase activity.
Asunto(s)
Antioxidantes/administración & dosificación , Ácido Ascórbico/administración & dosificación , Carotenoides/administración & dosificación , Melaninas/metabolismo , Pigmentación de la Piel/efectos de los fármacos , Vitamina E/administración & dosificación , Adulto , Dieta , Método Doble Ciego , Humanos , Persona de Mediana Edad , Piel/metabolismoRESUMEN
Substantial evidence supports the theory that free radicals, especially oxygen radicals, are involved in the process of aging. The human organisms have two ways to fight them: an enzymatic way with enzymatic intervention like superoxide dismutase, catalase... and a chemical way with the intervention of scavengers such as vitamins, cysteine, methionine, gluthatione... The aim of this work was to determine that an intakes of vitamins association: vitamin E, vitamin C and beta carotene induce an increase of singlet oxygen protection of erythrocytes' subjects. The method was based on the haemolytic effect of singlet oxygen which is generated by irradiation of hematoporphyrine at 365 nm, in 22 p. cent suspension of erythrocytes' subjects. Results show that a supply of beta carotene (15 or 30 mg/day), vitamin E (15 mg/day) and vitamin C (30 mg/day) involves an increase of singlet oxygen protection of erythrocytes of subjects. This protection appears very quickly after 15 days of treatment.
Asunto(s)
Ácido Ascórbico/farmacología , Carotenoides/farmacología , Eritrocitos/fisiología , Hemólisis , Oxígeno/farmacología , Vitamina E/farmacología , Administración Oral , Ácido Ascórbico/administración & dosificación , Carotenoides/administración & dosificación , Envejecimiento Eritrocítico , Eritrocitos/efectos de los fármacos , Eritrocitos/efectos de la radiación , Hematoporfirinas/efectos de la radiación , Hemólisis/efectos de los fármacos , Hemólisis/efectos de la radiación , Humanos , Fotoquímica , Valores de Referencia , Oxígeno Singlete , Rayos Ultravioleta , Vitamina E/administración & dosificación , beta CarotenoRESUMEN
OBJECTIVE: To evaluate the in vivo radical scavenger activity of vitamin E, vitamin C, and beta carotene on erythrocyte membranes. DESIGN: A prospective, open trial without placebo. SETTING: Department of Clinical Pharmacy. PATIENTS: Ten healthy volunteers being supplemented with beta carotene, vitamin E, and vitamin C. MEASUREMENTS: Erythrocytes were incubated in water bath with 2,2' azobis (2 amidinopropane) hydrochloride (AAPH). AAPH decomposes spontaneously at 37 degrees C to generate free radicals inducing membrane cellular damage and hemolysis. The absorbance was measured at 405 nm at 0, 30, and 60 min, and then every 20 minutes for four hours. The time for 50 percent of maximal hemolysis (T50%), which expresses the radical scavenger activity of erythrocytes, was determined. RESULTS: The physiologic T50% value determined in 52 healthy volunteers is 117 +/- 12 min. Patients receiving these supplements have a higher value of T50% (143.2 +/- 11.6 min at 30 d and 145.7 +/- 10.5 min at 60 d) than the physiologic value (p < 0.001). CONCLUSIONS: These data suggest that vitamin C, vitamin E, and beta carotene stimulate the radical scavenger activity of erythrocyte membranes after 30 days.
Asunto(s)
Ácido Ascórbico/farmacología , Carotenoides/farmacología , Membrana Eritrocítica/efectos de los fármacos , Depuradores de Radicales Libres , Vitamina E/farmacología , Amidinas , Ácido Ascórbico/administración & dosificación , Carotenoides/administración & dosificación , Membrana Eritrocítica/metabolismo , Humanos , Masculino , Estudios Prospectivos , Vitamina E/administración & dosificación , beta CarotenoRESUMEN
We have studied the metabolism (absorption) of beta-carotene and vitamin E by assigning eleven volunteers to receive daily two capsules of OENOBIOL, each containing 15 mg of beta-carotene and 15 mg of vitamin E, over 60 days. The beta-carotene, vitamin E and vitamin A plasma levels were then determined using new methods developed in our laboratory. After two months, the actively treated group's median beta-carotene and vitamin E levels were significantly higher than those of a control group. However, no significant change between treated and control groups in the mean of vitamin A (retinol) plasma levels were observed. Treatment with beta-carotene, a vitamin A precursor, does not significantly modify the vitamin A levels. This conclusion had already been observed and it is assumed that a plasma level of beta-carotene equal or higher than 0.3 mg/L reflects a nutritional intake of provitamins sufficient to support homeostasis of retinol (Brubacher et al., 1982).
Asunto(s)
Carotenoides/sangre , Vitamina A/sangre , Vitamina E/sangre , Absorción , Adulto , Carotenoides/farmacocinética , Cromatografía Líquida de Alta Presión , Femenino , Homeostasis , Humanos , Masculino , Vitamina E/farmacocinética , beta CarotenoRESUMEN
A small percentage of patients treated with bovine collagen implants have adverse reactions involving both the cellular and humoral types of immune response. We report a clinical follow-up of 705 subjects treated with a new bovine collagen implant, Atelocollagen (Koken Co. Ltd., Tokyo, Japan). Sensitization to the implant was evaluated in all subjects by skin testing, and humoral response was monitored in 166 subjects by measuring the level of circulating antibodies directed against bovine collagen. Twenty-seven patients (3.8%) exhibited a positive response to a skin test, and of the remaining 656 patients, an adverse reaction to the implant developed in 2.3%. We found a strong correlation between the presence of antibodies to collagen and a positive response to skin testing (92%) or an adverse reaction (100%). In the case of a borderline clinical response to bovine collagen implantation, anticollagen serologic tests appeared to be a useful tool for the identification of clinically reactive patients.