[The content of protein regulators of apoptosis Bcl-2 and bad in regional lymph nodes of the liver under the influence of 50 Hz a magnetic field].

There was investigated the expression of molecular and cellular regulators of apoptosis Bcl-2 and Bad in the regional lymph nodes of the liver in rats exposed to the magnetic field of 50 Hz during 14 days. There was revealed a reduction in the protein expression of regulators of apoptosis Bcl-2 in the cortical area of the regional lymph nodes of the liver in animals exposed to the magnetic field.

[The response of organelles of rat liver hepatocytes to the exposure of amplitude-modulated power frequency magnetic field].

There was investigated the response of cytoplasmic organelles of rat liver hepatocytes to the exposure of impact of power frequency magnetic field (magnetic field intensity: 5 - 10 microT, exposure time: 14 days). A statistically significant reduction in the number of hepatocytes and volume of mitochondria, the decline of the number of primary lysosomes and peroxisomes, the increase of granular and agranular reticulum has been established.

Structure of nuclear chromatin in cells of lymphoid organs and blood from mice maintained under abnormal light-dark cycle and treated with benz(a)pyrene.

We studied the effects of benzo(a)pyrene on lymphocyte chromatin in mice with experimental desynchronosis. Desynchronosis altered chromatin structure in immune system cells and suppressed the immune response. In mice with experimental desynchronosis, benzo(a)pyrene produced more pronounced changes in the chromatin structure compared to the control. It was inferred that light-dark cycle disturbances impair the resistance of organs and systems to damaging environmental factors.

[Micro- and ultrastructural characteristics of liver and nuclear endonuclease activity in hepatocytes after the combined exposure to industrial frequency magnetic field and continuous illumination].

The effects of combined action of the industrial frequency magnetic field (MF) and continuous illumination (CI) on microcirculation, ultrastructural features of liver cells and apoptosis were studied in Wistar rats (n=56). It was found that in the liver of rats exposed to MF, CI and CI+MF for 14 days, parenchyma relative area was reduced, and that occupied by the sinusoidal capillaries was increased together with the liver vascularization coefficient. Combined exposure resulted in most pronounced ultrastructural changes (plasma membrane lysis in hepatocytes and endothelial cells, doubling of osmiophilic layers in tight intercellular junctions). Hepatocytes revealed condensed cytoplasm, mitochondria with electron-dense matrix, nuclear chromatin disintegration and nucleolar compaction. Hepatocyte intranuclear endonucleases were activated.

Expression of intracellular molecular apoptosis regulator Bcl-2 in the liver in isolated and combined exposure to 24-h illumination and industrial frequency magnetic field.

We studied the effect of isolated and combined exposure to 24-h illumination and industrial frequency magnetic field on the expression of intracellular molecular apoptosis regulator Bcl-2 in the liver. Previous experiments showed enhanced expression of Bcl-2 protein in liver cells in animals maintained under conditions of 24-h illumination, whereas exposure to industrial frequency magnetic field had practically no effect on this process. Under conditions of combined exposure to 24-h illumination and industrial frequency magnetic field, magnetic field partially suppressed the expression of Bcl-2 induced by 24-h illumination.

[Changes in liver and in some organs of immune system of animals exposed to twenty-four-hour illumination].

The aim of this research was to study the morpho-functional organization of liver and some organs of immune system in animals exposed to continous twenty-four-hour illumination (CI) for 14 days. Using the methods of light and electron microscopy and morphometric analysis of histological preparations, liver and regional lymph nodes (hepatic in rats and iliac in mice) were studied. It was found that CI resulted in a state of dyschronosis, which was structurally manifested by the changes of diurnal variations of morphometric parameters of liver and its draining lymph nodes, disturbances of hemo- and lymphocirculation, destruction of cellular membranes. Response of central and peripheral organs of immune system to CI included the changes of diurnal variations of blast forms and mature lymphocytes, that was also indicative of dyschronosis development.

[Morpho-functional changes of liver and its regional lymph nodes after exposure to magnetic field of industrial frequency].

The aim of this research was to study the morpho-functional organization of liver and its regional lymph nodes, as well as of other organs of immune system, in animals exposed for 14 days to a magnetic field of industrial frequency (MFIF) with aperiodic oscillations of amplitude of magnetic induction in the range of 5-10 _Ts. The methods of light and electron microscopy were used. Damage to DNA in hepatocyte nuclei was determined using ethidium bromide. Quantitative analysis of the cells in the organs of immune system was performed, blood leukocyte formula was calculated and the activity of oxidative enzymes in blood cells was determined. It was found that MFIF caused micro- and ultrastructural changes in animal liver and its regional lymph nodes, which included the disturbances of hemo- and lymphocirculation, destruction of cellular membranes in some cells, dilation of rough endoplasmic reticulum cisterns, appearance of degenerate mitochondrial forms, glycogen depletion, that collectively indicated the development of tissue hypoxia. The increase in the activity of nuclear endonucleases was detected. Response of organs of immune system to MFIF was manifested by shifts of blast forms/ mature lymphocytes ratio in hepatic regional lymph nodes and by the changes of energy metabolism of blood lymphocytes.

[Effects of vibration on contents of micronuclei in polychromatophilic erythrocytes of the rat bone marrow]. / Vliianie vibratsii na soderzhanie mikroiader v polikhromatofil'nykh éritrotsitakh kostnogo mozga krys.

The paper presents data on the impact of vibration (32 Hz) on spontaneous mutagenesis in mammals. Higher counts of micronuclei were found in the bone marrow polychromophilic red blood cells in rats on day 5 of exposure to vibration, there was a reduction in their counts by day 20 and their recovery by day 30, and their steady-state decrease within 30 days after vibration discontinuation. The involvement of emotional stress in the mechanism of antimutagenic action of vibration is considered.