[Comparison of three methods to evaluate microalbuminuria in insulin-dependent diabetics: radioimmunoassay, laser-nephelometry and Laurell electroimmunodiffusion]. / Comparaison de trois techniques de dosage de la microalbuminurie chez les diabétiques insulino-dépendants: radioimmunologie, néphélémétrie-laser et électro-immuno-diffusion de Laurell.

The early detection of microalbuminuria in insulin dependent diabetes is considered as a sign of initial stage of nephropathy possibly reversible if glycemic balance is well maintained. This detection requires very accurate methods as radio-immuno-assays. Yet, they are so slow that they represent an obstacle to systemic detection. We report in this work an apparaisement of an immuno-nephelemetric and electro-immuno-diffusion of Laurell methods. Results reveal that immuno-nephelemetry and electro-immuno-diffusion are a sensitive and accurate methods (threshold of sensitivity of Laurell method is : 2.5 mg/l and nephelemetry is 1.5 mg/l). Moreover, RIA, immunonephelemetry and Laurell methods are significantly well correlated (r = 0.903: Laurell/Radio-immuno-assay, r = 0.907: Nephelemetry/Laurell). In conclusion immuno-nephelemetry and electro-immuno-diffusion of Laurell are a choice methods to test great lines of samples. Radio-immuno-assay can be used as a reference method of detection of microalbuminuria.