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Mater Sci Eng C Mater Biol Appl ; 33(3): 1315-24, 2013 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-23827577

RESUMEN

The surface properties of biomaterials, such as wettability, polar group distribution, and topography, play important roles in the behavior of cell adhesion and proliferation. Gaseous plasma discharges are among the most common means to modify the surface of a polymer without affecting its properties. Herein, we describe the surface modification of poly(styrene) (PS) and poly(methyl methacrylate) (PMMA) films using atmospheric pressure plasma processing through exposure to a dielectric barrier discharge (DBD). After treatment the film surface showed significant changes from hydrophobic to hydrophilic as the water contact angle decreasing from 95° to 37°. All plasma-treated films developed more hydrophilic surfaces compared to untreated films, although the reasons for the change in the surface properties of PS and PMMA differed, that is, the PS showed chemical changes and in the case of PMMA they were topographical. Excellent adhesion and cell proliferation were observed in all films. In vitro studies employing flow cytometry showed that the proliferation of L929 cells was higher in the film formed by a 1:1 mixture of PS/PMMA, which is consistent with the results of a previous study. These findings suggest better adhesion of L929 onto the 1:1 PS/PMMA modified film, indicating that this system is a new candidate biomaterial for tissue engineering.


Asunto(s)
Fibroblastos/citología , Gases em Plasma/farmacología , Polimetil Metacrilato/farmacología , Poliestirenos/farmacología , Naranja de Acridina/metabolismo , Animales , Adhesión Celular/efectos de los fármacos , Muerte Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Electricidad , Fibroblastos/efectos de los fármacos , Fibroblastos/ultraestructura , Citometría de Flujo , Técnica del Anticuerpo Fluorescente , Ratones , Microscopía de Fuerza Atómica , Termodinámica , Agua/química , Humectabilidad/efectos de los fármacos
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