Myocarditis in the common cuttlefish (Sepia officinalis).

This report describes five cases of myocarditis in the common cuttlefish, Sepia officinalis. Both the systemic heart and the branchial hearts exhibited inflammatory lesions. Vibrio species were isolated from four of these cases.

Development of newly formed nephrons in the goldfish kidney following hexachlorobutadiene-induced nephrotoxicity.

New nephrons developed in goldfish, Carassius auratus, several weeks following hexachlorobutadiene-induced (HCBD) nephrotoxicity. Basophilic clusters of presumptive nephrogenic cells incorporated 5-bromo, 2'deoxyuridine (BrdU) one week after HCBD injection, indicating initiation of DNA synthesis. These clusters, like renal vesicles in the developing kidney, elongated, fused with collecting ducts and developed into immature nephrons during the next 2 weeks. Stereologic quantification showed the volume percent of the kidney occupied by the developing nephrons was greater in HCBD-treated fish 2, 3, 4, and 10 weeks after injection than in the control fish. The presence of large numbers of developing nephrons may provide a marker for renal injury in fish from contaminated waterways.

Retinoblastoma in a porkfish (Anisotremus virginicus, Linnaeus) and a brown bullhead (Ictalurus nebulosus, Lesueur).

Two cases of retinoblastoma in fish are described. The neoplasms occurred in a porkfish (Anisotremus virginicus, Linnaeus) and in a brown bullhead (Ictalurus nebulosus, Lesueur).

Tropical fish medicine. Necropsy examination of fish.

Necropsy examination of moribund or dead specimens is an essential step in diagnosing fish diseases. This article discusses basic anatomy and necropsy procedures. A large part of the article has been devoted to methods of procuring samples for laboratory analysis and histologic examination. To determine which findings are responsible for the mortalities, the entire case history and gross necropsy findings must be reviewed. Careful observations made during the necropsy examination provide valuable information immediately, as well as later on in the interpretation of laboratory data.

Evaluation of oral administration of cortisol and metyrapone: the effects on serum cortisol in rainbow trout (Salmo gairdneri).

The effect of manipulating dietary levels of cortisol and metyrapone on peripheral concentrations of serum cortisol was examined in rainbow trout, Salmo gairdneri. Fish were fed 0, 10, 20, 30, 40 or 50 mg/kg body weight/day of metyrapone or cortisol for 7 days. All levels of cortisol tested increased peripheral levels of serum cortisol. Feeding a level of 30-40 mg/kg body weight/day of metyrapone depressed serum cortisol. We describe, herein, a noninvasive technique for suppression or stimulation of serum cortisol in rainbow trout.

The cellular immune response of rainbow trout (Salmo gairdneri Richardson) to sheep red blood cells.

The cellular immune response of rainbow trout, Salmo gairdneri, to sheep erythrocytes was investigated. Both the primary and secondary responses were measured using the migration inhibition factor (MIF), antigen-binding (ABC), and plaque-forming cell (PFC) assays. These immune function assays provide measures of both T and B cell activity. The kinetics of these three responses at 16 degrees C were determined by sampling fish over an 18 day period for the primary response and a ten day period for the secondary response. The peak MIF response occurred two days after injection, while the primary peak PFC response was observed 14 days post-injection. Two ABC peaks were observed in the primary response, one at four days and one at ten days after injection. In the secondary response the peak ABC response was observed four days and the peak PFC response six days post-inoculation. The possible interrelationships of the various cell populations are discussed.