RESUMEN
The effects on oocyte viability of varying the duration of exposure to cryoprotectants before rapid cooling to -196 degrees C were examined, using the vitrification protocol of Nakagata. A very short exposure (15 sec) was found to be optimal, resulting in an overall rate of development from vitrified oocytes to hatching blastocysts of 31.8%. Very high rates of survival (77-89%) of oocytes exposed to the cryoprotectant media, but without the vitrification, together with extreme variability in results between straws in the vitrified groups, suggest that losses in viability during vitrification may result from ice damage during devitrification of the medium.
Asunto(s)
Criopreservación/métodos , Crioprotectores/farmacología , Oocitos , Animales , Blastocisto , Supervivencia Celular , Desarrollo Embrionario y Fetal , Fertilización In Vitro , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Oocitos/efectos de los fármacos , Factores de TiempoRESUMEN
The purpose of this study was to evaluate the success of gamete intrafallopian transfer (GIFT) and superovulation with intra-uterine artificial insemination (S-IUI) in couples with long-term unexplained infertility. Thirty-nine couples were offered three attempts at each treatment. Sixty-three IUI cycles were completed with a pregnancy rate of 1.5% per cycle. Forty-nine GIFT procedures were carried out with a pregnancy rate of 26.5% per cycle. The cumulative conception rate after three cycles of GIFT was 0.56. GIFT appears to offer a far greater chance of pregnancy than S-IUI in unexplained infertility.
Asunto(s)
Transferencia Intrafalopiana del Gameto , Infertilidad/terapia , Inseminación Artificial Homóloga , Superovulación , Adulto , Buserelina/uso terapéutico , Clomifeno/uso terapéutico , Femenino , Humanos , Masculino , Menotropinas/uso terapéutico , Embarazo , ÚteroRESUMEN
Cardiovascular responsiveness to reflex impaired in patients with cirrhosis compared with control subjects. Peripheral vascular responses to exogenous noradrenaline were also impaired in cirrhotic patients, but peripheral vascular responses to infused adrenaline and to angiotensin II were similar in both groups. Impaired cardiovascular reactivity in patients with chronic liver disease could predispose them to circulatory failure after haemorrhage or surgery and should be considered when prescribing drugs which affect autonomic activity.