[Lactational incidences of production diseases in German Fleckvieh cows of six Bavarian dairy farms]. / Laktationsinzidenzen von Produktionskrankheiten bei Fleckviehkühen in sechs bayerischen Milchviehbetrieben.

OBJECTIVE: The aim of this study was to assess lactation incidences of production diseases in German Fleckvieh cows. MATERIAL AND METHODS: Investigations were carried out on six dairy farms (mean milk yield of herds 2008: 7834 ± 708kg milk [mean ± SD]) in Bavaria. All farms kept the cows in free stall barns and fed them a total or partial mixed ration based on grass silage and corn silage. In total, 116 cows and 58 heifers were examined daily for 14 days post partum and treated - if necessary - according to standard protocols. The acquisition of data for diseases in the further lactation was carried out by regular visits to the farm as well as communication with the herd manager and the farm veterinarian. RESULTS: Pluriparous cows suffered more frequently from production diseases (milk fever, retained placenta, clinical ketosis, abomasal displacement, metritis, endometritis, ovarian cysts, mastitis) than primiparous heifers: 33.3% and 46.4% of pluriparous and primiparous cows, respectively, remained clinically healthy, while 24.8% and 30.4%, respectively, suffered from one production disease during the first 2 weeks of lactation; more than one production disease was diagnosed in 41.9% and 23.2% of pluriparous and primiparous cows, respectively. The lactation incidences of production diseases varied considerably among pluriparous cows of the six farms: retained placenta 16.8 ± 13.2%, milk fever 15.1 ± 7.0%, clinical ketosis 16.8 ± 12.4%, metritis 3.8 ± 3.1%, abomasal displacement 1.1% (median 0.0; 0.0/0.0%), endometritis 11.7 ± 7.0%. Mastitis affected 56.0 ± 7.4% of the pluriparous cows, which experienced 1.7 mastitis episodes on average. At least one follicular cyst was diagnosed among 28.4 ± 8.6% of the cows. Lameness affected 18.5 ± 13.5% of pluriparous cows and heifers during the first 2 weeks of lactation. CONCLUSION: The lactation incidences of production diseases did not significantly differ from reference values reported for Holstein Friesian cows except the lower incidence of LDA among German Fleckvieh cows. The results indicate that the farm management affected lactation incidences of production diseases to a greater degree than additional factors, such as the breed of the cows.

Equus caballus papillomavirus-2 (EcPV-2): an infectious cause for equine genital cancer?

REASONS FOR PERFORMING STUDY: The aetiology of genital squamous cell carcinoma (SCC) in horses remains unknown, but the similarity to the disease in man, for which papillomavirus infection has been shown to be a causal factor, requires to be investigated in horses. HYPOTHESIS: One or more novel papillomaviruses cause equine genital SCC and its associated premalignant lesions. METHODS: DNA was extracted from samples of equine genital SCC and performed rolling circle amplification, in order to identify closed circular DNA viral genomes within the samples. The amplified DNA was subcloned and sequenced and the DNA sequence compared to that of other papillomavirus genomes. Using PCR primers developed from these genomic DNA sequences, studies were then carried out in order to identify the frequency at which the viral DNA could be identified in equine genital cancer samples from horses in both the UK, Australia and Austria. Finally, in situ hybridisation using specific probes developed from this DNA sequence were used to confirm the presence of the viral RNA sequences in the neoplastic cells in these lesions. RESULTS: The full length genome of a novel papillomavirus species was characterised from the equine genital SCC tissue and termed Equus caballus papillomavirus-2 (EcPV-2). Viral DNA and RNA was identified in the genital tumour samples, but not in the adjacent histologically normal tissue. EcPV-2 DNA could not be identified in equine ocular or nasal carcinomas or within the scrotal skin or in most smegma samples obtained from tumour-free horses. Sequencing of amplicons, generated from the archived equine genital tumours, identified variations within E1 and E6 on DNA and predicted protein level. CONCLUSIONS: A novel papillomavirus, EcPV-2, is likely to play a causal role in the pathogenesis of equine genital epithelial tumours. POTENTIAL RELEVANCE: Identification of a papillomavirus causal for genital carcinomas in horses may lead to development of a vaccine that could be used to prevent this serious disease in horses. This would be analogous to man, where vaccination against oncogenic papillomavirus species is currently being used to help prevent cervical cancer.