[Ultrastructural analysis of Pseudomonas aeruginosa invasion into the blood capillaries of the subcutaneous fat]. / Ul'trastrukturnyi analiz invazii sinegnoinoi palochki v krovenosnye kapilliary podkozhnoi kletchatki.

Wound wall soft tissues from 14 male rats were studied electron-microscopically 5 hours after subcutaneous implantation of a gauze piece with 3 X 10(10) organisms of Ps. aeruginosa on it. In a special series of experiments done on 75 rats, 78% of the animals have demonstrated a generalized pseudomonal infection, i. e. sepsis. The bacterial invasion into the blood stream occurred on the microcirculatory level due to inadequacy of the phagocytic barrier caused by bacterial damage of micro and macrophages. Vascular wall component destruction and capillary haemostasis (stasis, thrombosis) appeared to predispose the microbial invasion into the vascular lumen. The bacteria entered the circulation by the microembolic mechanism.

[Structural bases for the invasion of the causative agents of experimental Pseudomonas aeruginosa sepsis into the bloodstream from the primary septic focus]. / Strukturnye osnovy invazii vozbuditeleiéksperimental'nogo sinegnoinogo sepsisa v krovenosnoe ruslo iz pervichnogo septicheskogo ochaga.

The soft tissues around the implant with a suspension of P. aeruginosa were studied by light and electron microscopy. Severe damages to structures of the histohematic barrier, phagocytes were revealed as was the bacterial invasion into thrombosed vessels of the microcirculatory bed. The mechanism of the micrometabolism as playing the key role in the bacterial penetration from the primary septic focus into the common bloodstream is discussed.

[Ultrastructural analysis of changes in the lung parenchyma in the initial stages of experimental sepsis]. / Ul'trastrukturnyi analiz izmenenii parenkhimy legkikh v nachal'noi stadii éksperimental'nogo sepsisa.

Histological and ultrastructural alterations in the lung parenchyma of rats with sepsis induced by implantation of 3 X 10(10) microorganisms (P. aeruginosa) beneath the skin were studied. Microorganisms with micropili were detected in septal spaces and in the alveoli 10 hours after implantation. Their effects were linked with microcirculatory alterations and destruction of endotheliocytes, alveolocytes and septal cells.

[Mechanisms of development of pneumonia after burns]. / O mekhanizmakh razvitiia posleozhogovoi pnevmonii.

Experiments in rats (burns followed by intratracheal infection with a staphylococcus suspension) were used to study association between disorders in phagocytosis of polymorphonuclear leukocytes and alveolar macrophages and histological manifestations of the inflammatory-infectious process in the lungs. Different resistance of micro- and macrophage cells to burn trauma was demonstrated. Disorders in the phagocytic properties of the cells were shown to precede the development of the inflammatory-infectious process documented at the tissue level.