RESUMEN
Antennally active nonhost angiosperm bark volatiles were tested for their ability to reduce the response of three common species of coniferophagous wood-boring Cerambycidae to attractant-baited multiple funnel traps in the southern interior of British Columbia. Of the nonhost volatiles tested, only conophthorin was behaviorally active, disrupting the attraction of sawyer beetles, Monochamus spp., to traps baited with the host volatiles alpha-pinene and ethanol and the bark beetle pheromones ipsenol and ipsdienol. Conophthorin did not affect the attraction of sawyer beetles to the host kairomones alpha-pinene and ethanol in the absence of bark beetle pheromones, nor did it have any behavioral effect on adults of Xylotrechus longitarsis, which were not attracted to bark beetle pheromones. These results indicate that conophthorin does not act as a general repellent for coniferophagous Cerambycidae, as it seems to do for many species of Scolytidae, but has the specific activity of disrupting the kairomonal response of sawyer beetles to bark beetle pheromones.
Asunto(s)
Escarabajos , Feromonas/farmacología , Compuestos de Espiro/farmacología , Animales , Magnoliopsida/química , Movimiento , Extractos Vegetales/farmacología , Plantas Comestibles , Tracheophyta/química , VolatilizaciónRESUMEN
Volatile compounds released by disturbed and calm female and male Lygus lineolaris were collected and analyzed. Six major compounds were present in samples from disturbed bugs and from calm females: (E)-2-hexenal, 1-hexanol, (E)-2-hexenol, hexyl butyrate, (E)-2-hexenyl butyrate, and (E)-2,4-oxohexenal. (E)-2-hexenal was lacking in volatiles collected from calm males. Hexyl butyrate accounted for approximately 68% and 66% of volatiles released by agitated and calm females, and 87% and 88% of volatiles released by agitated and calm males, respectively. Blends released by disturbed insects differed quantitatively from blends released by calm insects, with amounts of compounds increasing 75-350 times in samples from disturbed insects. In static air bioassays, both females and males were repelled by natural volatiles collected from females and by five-component [(E)-2,4-oxohexenal excluded] and six-component synthetic blends at doses of 1 and 10 bug-hours, indicating that these volatiles may serve an alarm or epideictic function, as well as a possible role as defensive allomones. Adults also avoided hexyl butyrate, (E)-2-hexenyl butyrate, (E)-2-hexenol, and (E)-2,4-oxohexenal, but not 1-hexanol and (E)-2-hexenal when compounds were assayed individually in static air bioassays at doses equal to 1 bug-hour. When tested over 1 day in two-choice cage trials, adults did not prefer untreated bean plants over bean plants surrounded by vials releasing up to 8.1 mg/hr (= 234 bug-hours) of the five-component synthetic blend. Therefore, the volatiles produced by disturbed adults would not be useful as a repellent for L. lineolaris.
Asunto(s)
Miedo , Hemípteros/química , Feromonas/análisis , Adaptación Fisiológica , Animales , Bioensayo , Femenino , Hemípteros/fisiología , Masculino , Movimiento , Dinámica Poblacional , VolatilizaciónRESUMEN
We investigated the hypothesis that wood-boring beetles in the genus Monochamus (Cerambycidae) utilize pheromones of sympatric bark beetles as host-finding kairomones. All nine bark beetle pheromones tested electrophysiologically were antenally active for both sexes of M. scutellatus, M. clamator, and M. obtusus from British Columbia. When field-tested with multiple-funnel traps (British Columbia) or cross-vane traps (Ontario), a blend composed of frontalin, ipsdienol, ipsenol, and MCH, in combination with a blend of host volatiles attracted significant numbers of M. clamator, M. obtusus, M. notatus, and M. scutellatus to baited traps. Traps baited with host volatiles in combination with a second blend composed of endo-brevicomin, exo-brevicomin, cis-verbenol, trans-verbenol, and verbenone caught no more beetles than unbaited traps or traps baited with the host blend alone. In British Columbia, traps baited with the first blend alone or both blends together captured more M. scutellatus and M. clamator than unbaited traps, demonstrating a response to bark beetle pheromones in the absence of host volatiles. These results suggest that Monochamus spp. are minimizing foraging costs by using the pheromones of sympatric bark beetles as kairomones.
Asunto(s)
Escarabajos/fisiología , Conducta Alimentaria , Feromonas , Animales , Conducta Animal , Evolución Biológica , Olfato , Árboles , VolatilizaciónRESUMEN
The impact of feeding by the western conifer seed bug (Leptoglossus occidentalis Heidemann) on storage reserves of developing seeds of Douglas-fir (Pseudostuga menziesii (Mirb.) Franco) was studied by caging nymphal and adult seed bugs on cones during late-season development, and nymphs on cones during early, mid- and late-season development. Analysis of the major storage reserves of partially damaged seeds revealed that late-season feeding by each life stage and feeding by nymphs at all three stages of cone development significantly reduced the amounts of lipid and buffer-insoluble (crystalloid) protein in seeds at harvest by up to 78 and 97%, respectively. Seeds showing light to moderate damage on radiographs did not exhibit a reduction in the amount of buffer-soluble (matrix) protein. Seeds damaged by feeding during early development compensated in part by continuing to synthesize lipid and crystalloid protein. Light or moderate damage to mature Douglas-fir seeds exposed to L. occidentalis in the laboratory reduced seedling emergence by > 80%, but the seedlings that emerged successfully appeared to suffer no adverse effects when grown under standard nursery conditions.
Asunto(s)
Hemípteros , Pseudotsuga/fisiología , Semillas/fisiología , Árboles/fisiología , Animales , Lípidos/fisiología , Proteínas de Plantas/fisiología , Pseudotsuga/crecimiento & desarrollo , Pseudotsuga/parasitología , Semillas/crecimiento & desarrollo , Árboles/crecimiento & desarrolloRESUMEN
Specific biochemical marker-based techniques were tested for their ability to distinguish between seeds of Douglas-fir, Pseudotsuga menziesii (Mirbel) Franco, that were filled or unfilled (aborted) at maturity and those that were damaged or emptied by the western conifer seed bug, Leptoglossus occidentalis Heidemann. A polyclonal antibody raised against salivary gland extracts from L. occidentalis successfully identified residual salivary proteins on Western blots containing proteins from Douglas-fir seeds that had sustained various degrees of seed bug feeding damage. In a single blind experiment, the polyclonal antibody correctly identified 100% of undamaged control, 97% of unfilled control (aborted), and 98% of seed bug damaged seeds. Polyclonal antibodies raised against insoluble alfalfa crystalloid storage protein (11S globulin) detected the depletion of 11S globulin and the subsequent appearance of its hydrolyzed fragments in the soluble protein fraction of Douglas-fir seeds that were fed-upon by the seed bug. Feeding by L. occidentalis nymphs caused ca. 98% depletion of insoluble protein, but only ca. 53% reduction in the amount of soluble protein in seeds that appeared empty on radiographs. By comparison, unfilled (aborted) seeds contained significantly less insoluble and soluble protein than empty seeds that were fed-upon by L. occidentalis; moreover, no crystalloid (11S globulin) breakdown products were generated. The biochemical markers described in this study are reliable tools that can be used to identify conifer seeds that have sustained light to severe damage from L. occidentalis feeding.
Asunto(s)
Hemípteros , Árboles/metabolismo , Animales , Biomarcadores , Western Blotting , Electroforesis en Gel de Poliacrilamida , Conducta Alimentaria , Germinación , Hemípteros/metabolismo , Hemípteros/fisiología , Hidrólisis , Proteínas de Plantas/metabolismo , Saliva/metabolismo , Glándulas Salivales/metabolismo , Semillas/metabolismo , Dodecil Sulfato de SodioRESUMEN
Antennally active, bark-derived, angiosperm volatiles were tested singly and in groups for their ability to disrupt the response of the Douglas-fir beetle (DFB), Dendroctonus pseudotsugae, to attractant-baited multiple-funnel traps. One compound, conophthorin, was active alone in reducing the response of beetles to the baited traps. Further experiments showed disruptive activity in two aliphatic green-leaf alcohols [1-hexanol and (Z)-3-hexen-1-ol], as well as guaiacol and benzyl alcohol, and three aliphatic aldehydes [nonanal, hexanal, and (E)-2-hexenal] but not in two aromatic aldehydes (benzaldehyde and salicylaldehyde). Every binary combination that included conophthorin or any two of the other groups, except aromatic aldehydes, significantly reduced the response of beetles to baited traps. Various ternary mixtures and the complete mixture of all the groups were generally the most effective treatments. These results provide evidence that DFBs recognize and avoid nonhosts while flying rather than landing on candidate hosts and testing them while in contact with the tree. Nonhost angiosperm bark volatiles may have practical utility on their own or in combination with the antiaggregation pheromone 3-methylcyclohex-3-en-1-one (MCH) to protect single trees, logs, or stands from attack by the DFB.
Asunto(s)
Escarabajos/fisiología , Magnoliopsida/química , Feromonas/farmacología , Árboles/química , Alcoholes/farmacología , Aldehídos/farmacología , Animales , Escarabajos/efectos de los fármacos , Conducta Alimentaria/efectos de los fármacos , Conducta Alimentaria/fisiología , Femenino , Control de Insectos/métodos , Masculino , Corteza de la Planta/química , Compuestos de Espiro/farmacología , VolatilizaciónRESUMEN
We investigated the effect of feeding by the western conifer-seed bug, Leptoglossus occidentalis Heidemann, on seed production in developing cones of coastal Douglas-fir, Pseudostuga menziesii (Mirb.) Franco, with respect to seed bug life stage and sex (nymphs, adult females, and adult males) and timing of feeding (early, mid-, and late season cone development). Feeding by females on caged cones for a 2-wk period during late season cone development reduced the proportion of full seeds in cones by approximately 70% compared with caged control cones. There was no significant difference among nymphs, adult females, and adult males with respect to the proportion of empty or partially fed-upon seeds produced during the same feeding period. Feeding by nymphs for 2 wk early in the season resulted in a threefold increase in the number of unextractable seeds fused to cones compared with the control. Weight measurements of harvested seeds indicated that radiography is an accurate tool to distinguish among Douglas-fir seeds that have sustained light, moderate, or severe damage. Determining the full impact of L. occidentalis on conifer seed production will require the development of a reliable method to distinguish between naturally aborted seeds and seeds emptied through feeding by seed bugs.
Asunto(s)
Hemípteros/fisiología , Árboles/crecimiento & desarrollo , Animales , Conducta Alimentaria , Femenino , Hemípteros/crecimiento & desarrollo , Masculino , SemillasRESUMEN
Induced (traumatic) resin in white spruce (Picea glauca (Moench) Voss) leaders resistant or susceptible to the white pine weevil (Pissodes strobi Peck) was analyzed for volatile terpenes and diterpene resin acids after simulated white pine weevil damage. Leaders from 331 trees were wounded just below the apical bud with a 1-mm diameter drill, coinciding with the natural time of weevil oviposition in the spring. Leaders were removed in the fall, and the bark and xylem from the upper and lower regions of the leader extracted and analyzed by gas chromatography. Unwounded trees had low amounts of resin in xylem compared with bark. In response to wounding, volatile terpenes and diterpene resin acids increased in the upper xylem (area of wounding), with resistant trees showing a greater increase than susceptible trees. Wounding caused monoterpenes in particular to decrease in the lower region of the leader (away from the drilled area) in greater amounts in susceptible trees than in resistant trees. In response to wounding, the proportion of monoterpene to resin acid increased in the upper and lower xylem of resistant trees, and slightly increased in the upper xylem of susceptible trees. Monoterpene-enriched resin is more fluid than constitutive resin, and probably flows more readily into oviposition cavities and larval mines, where it may kill immature weevils. Loss of resin components in the lower xylem suggested catabolism and transport of these materials to the site of wounding; however, energetic and regulatory data are necessary to confirm this hypothesis. This study provides a basis for measuring the ability of a tree to undergo traumatic resinosis that could be used to screen for resistance to white pine weevil.
Asunto(s)
Escarabajos , Diterpenos/análisis , Enfermedades de las Plantas , Resinas de Plantas/química , Terpenos/análisis , Árboles/fisiología , Animales , Árboles/químicaRESUMEN
Foliage from the pepper tree,Schinus molle L., is traditionally used in Ethiopia to "repel" house flies,Musca domestica L. The volatile extracts of pepper tree leaves were shown to have repellent and feeding-deterrent activity against house flies in a two-choice laboratory bioassay. High-performance liquid chromatographic fractionation of steam-distilled volatiles from leaves, monitored by laboratory bioassays, demonstrated that bioactivity is associated with two compounds,cis-menth-2-en-1-ol andtrans-piperitol. The absolute configuration of the latter was established as (1S,6S)-piperitol by comparison of acetyl lactate derivatives. Racemic compounds were synthesized from piperitone, and bioassays with house flies indicatedtrans-piperitol to be the most active house fly repellent.
RESUMEN
We tested the hypothesis that green leaf volatiles act as antiaggregants for the mountain pine beetle (MPB),Dendroctonus ponderosac Hopkins. In coupled gas chromatographic-electroantennographic detection (GC-EAD) analysis MPB antennae responded to 30 ng doses of all six-carbon green leaf alcohols tested [1-hexanol, (E)-2-hexen-1-ol, (Z)-2-hexen-1-ol, (E)-3-hexen-1-ol, and (Z)-3-hexen-1-ol], but not to the aldehydes, hexanal or (E)-2-hexenal, or to alcohol or aldehyde homologues with more or fewer than six carbon atoms. In field trapping experiments a blend of green leaf alcohols [1-hexanol, (Z)-2-hexen-1-ol, (E)-3-hexen-1-ol and (Z)-3-hexen-1-ol] effectively disrupted the response to attractive semiochemicals; a blend of the aldehydes hexanal and (E)-2-hexenal was inactive. The two best disruptants. (E)-2-hexen-1-ol and (Z)-3-hexen-1-ol, reduced catches of both sexes to levels not significantly different from catches in unbaited control traps. They also reduced the attack on trees baited with attractive MBP pheromones to a level not significantly different from that on unbaited control trees. Neither of the clerid predators captured,Enoclerus sphegeus (F.) norThanasimus undatulus (Say), was repelled by green leaf volatiles. Our results suggest that green leaf alcohols are promising disruptants which may be used to supplement the antiaggregation pheromone, verbenone, in protecting single high-value trees as well as carefully selected stands with low-level populations of MPBs.
RESUMEN
Analyses of the enantiomeric composition of ipsdienol produced by individual male pine engravers,Ips pini (Say), from six populations in British Columbia, support the hypothesis that New York and Idaho races of this species hybridize in southeastern British Columbia. Production profiles, expressed as frequency distributions of (+):(-) ipsdienol ratios [= ratio of (S)-(+)-ipsdienol to (R)-(-)-ipsdienol], were bimodal for four western British Columbia populations. The (+):(-) ratios ranged from 63:37 to 71:29. consistent with those previously found for the New York race. The profile for a southeastern population from Radium, British Columbia, was intermediate between those for the four western British Columbia populations and that from one population in Kimberley, British Columbia, just south of Radium. Males in the Kimberley population produce predominantly (R)-(-)-ipsdienol, typical of California nad Idaho males. Response profiles of different individuals ofI. pini, determined by captures of beetles in multiple-funnel traps baited with ipsdienol of 11 different (+):(-) ratios, were not consistent with production profiles. Populations in Williams Lake and Princeton, in western British Columbia, and Radium, in southeastern British Columbia, had response profiles with maximal attraction to ipsdienol over a broad range of (+):(-) ratios, falling off as enantiomeric purity was approached at either end of the spectrum. This type of response profile is consistent with that for the New York race, which has been shown to respond optimally to (+):(-) ratios ranging from 40:60 to 70:30. The response profile of the Kimberley population gradually declined from maximal attraction to ipsdienol with a (+):(-) ratio of 2:98 to the lowest response at a (+):(-) ratio of 98:2. The attraction ofI. pini to chemical stimuli in California is interrupted by ipsdienol with a (+):(-) ratio >5:95, a pheromone of a host competitor, the California five-spined ips,Ips paraconfusus Lanier. We hypothesie that the Idaho race, which does not compete withI. paraconfusus due to geographical separation, is characterized by a Kimberley-type enantiomeric response profile, intermediate between those of the New York and California races.
RESUMEN
Production of 4-methyl-5-nonanol, and 4-methyl-5-nonanone by two sympatric Asian palm weevils,Rhynchophorus ferrugineus (Oliv.) andR. vulneratus (Panz.) suggested that enantiospecificity of either compound could impart species specificity of pheromone communication. Weevil-produced, racemic 4-methyl-5-nonanol and 4-methyl-5-nonanone and their stereoselectively synthesized optical isomers were subjected to gas chromatographic-electroantennographic detection (GC-EAD) and GC-mass spectrometry (MS) on a chiral Cyclodex-B column. Only theS,S stereoisomer of 4-methyl-5-nonanol was EAD active and was produced by bothR. ferrugineus andR. vulneratus. Production and EAD activity of (S)-4-methyl-5-nonanone exceeded that of its antipode in both weevils. In field experiments in Java. (4S, 5S)-4-methyl-5-nonanol and the stereoisomeric mixture were equally attractive. The 4R,5R stereoisomer was inactive. The corresponding ketone enantiomers neither enhanced nor reduced attraction to (4S,5S)-4-methyl-5-nonanol. Lack of apparent differences betweenR. ferrugineus andR. vulneratus pheromones suggests that synonomy of both weevils should be considered unless other pre- or postzygotic reproductive isolating mechanisms are disclosed in future studies.
RESUMEN
A two-choice feeding bioassay was used to investigate the intra-specific repellency of the larval oral exudate of eastern and western spruce budworms,Choristoneura fumiferana (Clem.) andC. occidentalis Free., respectively. Results of the bioassay indicated that feeding behavior on artificial diet-drop feeding stations was modified in the presence of exudate, with feeding stations treated with conspecific exudate being avoided when an untreated station was available 3 cm away. Feeding was suppressed when a single, exudate-treated station was provided, or when the treated and untreated stations were separated by only 1 cm. The repellent effect functioned both inter- and intraspecifically. When induced to produce exudate,C. occidentalis larvae were not immediately repelled by either their own or other individuals' exudate. However, 24 hr after induction, test larvae were repelled by exudate from either source. In both species, larval oral exudate probably functions to repel conspecific competitors.
RESUMEN
Male coconut rhinoceros beetles,Oryctes rhinoceros (L.), produce three sex-specific compounds, ethyl 4-methyloctanoate, ethyl 4-methylheptanoate, and 4-methyloctanoic acid, the first of which is an aggregation pheromone. Synthesis of these compounds involving conjugate addition of organocuprates to ethyl acrylate is reported. In field trapping experiments, (4S)-ethyl 4-methyloctanoate and the racemic mixture were equally attractive and 10 times more effective in attracting beetles than ethyl chrysanthemumate, a previously recommended attractant. Ethyl 4-methylheptanoate was as attractive as ethyl chrysanthemumate and more attractive than 4-methyloctanoic acid, but further studies are required before it can be classed as an aggregation pheromone. Compared to ethyl 4-methyloctanoate alone, combinations of the three male-produced compounds did not increase attraction, whereas addition of freshly rotting oil palm fruit bunches to pheromone-baited traps significantly enhanced attraction. With increasing dose, captures ofO. rhinoceros increased, but doses of 6, 9, and 18 mg/day were competitive with 30 mg/day lures. Newly designed vane traps were more effective in capturing beetles than were barrier or pitfall traps. Results of this study indicate that there is potential for using ethyl 4-methyloctanoate in operational programs to controlO. rhinoceros in oil palm plantations.
RESUMEN
Trapping experiments were conducted in orchards to test the hypothesis that exposure of the mullein bug,Campylomma verbasci (Meyer), to atmospheres permeated with its synthetic sex pheromone, a 94:6 blend of butyl butyrate (BB) and (E)-crotyl butyrate (CB), or BB or CB alone, would alter the pheromone responses of males toward off-ratio blends. Exposure to the natural 94:6 blend shifted the response preference away from the natural ratio to blends enriched in BB, including a 99:1 blend, which is normally significantly less attractive than the natural ratio. In an atmosphere permeated with CB, male mullein bugs were as responsive to blends containing 20, 33, and 43% CB, up to seven times the natural percentage, as they were to the 94:6 blend. In an atmosphere permeated with BB, responses to five blends ranging from 94:6 to 99:1 BB:CB were not significantly different, whereas in an untreated atmosphere blends of 98:2 and 99:1 were significantly less attractive than the 94:6 blend. Individual components appeared to disrupt pheromone communication primarily through the creation of a sensory imbalance and modified interpretation of pheromone blend ratios, whereas disruption by the complete pheromone blend apparently involves several mechanisms, including false-trail following and camouflage. Disruption of pheromone communication was most effective with the natural 94:6 sex pheromone blend.
RESUMEN
Female white pine cone beetles,Conophthorus coniperda, attacking second-year cones of eastern white pine,Pinus strobus L., produced a sex-specific pheromone that attracted conspecific males in laboratory bioassays and to field traps. Beetle response was enhanced by host monoterpenes. The female-produced compound was identified in volatiles collected on Porapak Q and in hindgut extracts as (+)-trans-pityol, (2R,5S)-(+)-2-(1-hydroxy-1-methylethyl)-5-methyltetrahydrofuran. Males and females produced and released the (E)-(-)-spiroacetal, (5S,7S)-(-)-7-methyl-1,6-dioxaspiro[4.5]decane, which was not an attractant for either sex, but acted as a repellent for males. Porapak Q-trapped volatiles from both sexes contained (+)-trans-pinocarveol and (-)-myrtenol. In addition, hindgut extracts of females containedtrans-verbenol, while males had pinocarvone and verbenone. Work in Georgia and Canada confirmed that the same isomers of pityol and spiroacetal are present in two distinct and widely separated populations ofC. coniperda.
RESUMEN
Analyses of abdominal extracts and beetle-produced volatiles revealed that femaleConophthorus resinosae andC. banksianae produced optically pure (E)-(+)-pityol [(2R,5S)-(+)-2-(1-hydroxy-1-methylethyl)-5-methyltetrahydrofuran]. In field tests, traps baited with (E)-(±)- or (E)-(+)-pityol captured only males. Addition of host oils to traps baited with pityol did not significantly enhance the capture of males. Males of these former sibling species produced the spiroacetal (5S,7S)-(-)-7-methyl-1,6-dioxaspiro[4.5]decane with high optical purity (96%). Addition of the racemate or the optical isomers of the spiroacetal to traps baited with (E)-(±)-pityol almost completely inhibited the capture of males. It is hypothesized that to ensure their reproductive success, males produce the spiroacetal to repel rival males. Species specificity in pityol and the spiroacetal was not found betweenC. resinosae andC. banksianae, thus supporting their synonymy.
RESUMEN
Chemical analysis of whole body extracts and volatiles produced by feeding malesDryocoetes affaber (Mann.) disclosed (+)-exo-brevicomin and (+)-endo-brevicomin [(+)EXOB and (+)ENDOB], as the major insect-produced potential pheromones. Laboratory bioassays and field-trapping experiments demonstrated that (+)ENDOB is the main pheromone component, and (-)ENDOB has an inhibiting effect. EXOB either as (+) or (±) appears to be a multifunctional pheromone. It has a synergistic effect in blends of EXOB and ENDOB in ratios up to 1:1, and it is inhibitory at higher ratios. (-)EXOB was inactive. The most attractive blend forD. affaber was a 1:2 blend of (+)EXOB and (+)ENDOB. When this blend was compared with a 9:1 blend, the best known blend forDryocoetes confusus Swaine, the responses by beetles of each of the two species were highly specific, providing evidence for pheromonal exclusion between the two congenerics. We conclude that the combined effect of chirality and the ratio of geometrical isomers of brevicomin determines both the level of response and the species-specificity of the chemical signal inD. affaber.