[Characteristics of primary cell culture from neonatal thyroid grand of pigs: folliculogenesis, hormone and growth].

In the study, comprehensive assessment of proliferative and hormonal activity of primary cell cultures derived from neonatal pig thyroid has been carried out for the first time. We have evaluated the basal and TSH-stimulated secretion of thyroxine and morphological features of culture, depending on the initial state of the material placed in culture: in the form of single cells or follicular conglomerates. Folliculogenesis and formation of dome structures were observed in culture spontaneous and under chronic TSH stimulation. The ability of the cells to expression of ß-III-tubulin during prolonged cultivation in the presence of NGF has been demonstrated in the study.

[Estimation of luminescent properties of the derivatives of polymethine probes on their interaction with cells of different types].

The spectral and luminescent properties of the derivatives of polymethine probes based on 3,3'-dialkyloxacarbocyanine bromide (H-510) and 1,1'-dialkyl-3,3, 3',3'-tetramethylindodicarbocyanine bromide (D-307) (where alkyl is ethyl or octadecyl groups) on accumulation in bone marrow and liver cells of rats have been compared. The general regularities in the interaction between the probes and the membrane microenvironment have been revealed for each type of the cells. It was found that the structural features of membranes and the length of the chains of alkyl substitutes essentially affect the redistribution of dye molecules in the binding censers of particular types, as evidenced by changes in the spectral characteristics of probes. It has been shown by microfluorimetry that the accumulation of short-chain derivatives in single cells is representative of the distinctions in the parameters of their functional metabolic state, which coincides with the present view of the heterogeneous structure of these cellular populations. The hormonal action and the influence of incubation without serum on the intensity of cellular processes were accompanied by changes in the fluorescence of cells stained with the ethyl derivatives of the probes H-510 and D-307. This fact allows us to consider them as optical indicators of the cellular activity. The dye D-307 was found to be more resistant to photodestruction than H-510 both in single cells and model system.

Salivary analysis of oral cancer biomarkers.

BACKGROUND: Oral cancer is a common and lethal malignancy. Direct contact between saliva and the oral cancer lesion makes measurement of tumour markers in saliva an attractive alternative to serum testing. METHODS: We tested 19 tongue cancer patients, measuring the levels of 8 salivary markers related to oxidative stress, DNA repair, carcinogenesis, metastasis and cellular proliferation and death. RESULTS: Five markers increased in cancer patients by 39-246%: carbonyls, lactate dehydrogenase, metalloproteinase-9 (MMP-9), Ki67 and Cyclin D1 (CycD1) (P< or =0.01). Three markers decreased by 16-29%: 8-oxoguanine DNA glycosylase, phosphorylated-Src and mammary serine protease inhibitor (Maspin) (P< or =0.01). Increase in salivary carbonyls was profound (by 246%, P=0.012); alterations in CycD1 (87% increase, P=0.000006) and Maspin (29% decrease, P=0.007) were especially significant. Sensitivity values of these eight analysed markers ranged from 58% to 100%; specificity values ranged from 42% to 100%. Both values were especially high for the CycD1 and Maspin markers, 100% for each value of each marker. These were also high for carbonyls, 90% and 80%, respectively, and for MMP-9, 100% and 79%, respectively. CONCLUSION: The significance of each salivary alteration is discussed. As all alterations correlated with each other, they may belong to a single carcinogenetic network. Cancer-related changes in salivary tumour markers may be used as a diagnostic tool for diagnosis, prognosis and post-operative monitoring.

[Accumulation of oxacarbocyanine dyes with different length of the alkyl chain in marrow cells and hepatocytes].

The incorporation of the fluorescent probe 3,3-dialkyloxacarbocyanine bromide H-510 (where alkyl is ethyl- (C2), nonyl- (C9), or octadecyl (C18) groups) into cells of different kind has been explored. It has been revealed that the length of alkyl chains significantly influences the dynamics and mechanisms of accumulation of the probe by the cells. It has been found by microfluorimetry that all probe species have similar spectral characteristics in bone marrow cells, indicating that all probes, independently on their lipophilic properties, are incorporated into micelle-like structures formed probably by cell phospholipids. Spectroscopy experiments have shown that, in hepatocytes, the fluorescent probes 3,3-diethyloxacarbocyanine bromide (H-510/C2) and 3,3-dinonyloxacarbocyanine bromide (H-510/C9) are mainly accumulated in weakly polar media (nonpolar and weakly polar lipids of these cells). The luminescence maximum of the H-510/C18 probe in hepatocytes is blue-shifted and coincides with that in an albumin solution. We suppose that the incorporation of the probe into cells occurs by endocytosis when the probe binds to surface proteins.