RESUMEN
OBJECTIVE: To assess preptin concentrations in pregnant women with and without gestational diabetes mellitus (GDM) and in the cord blood of their fetuses. STUDY DESIGN: In all, 31 pregnant women with GDM and 31 gestational age-matched healthy pregnant subjects participated. Maternal serum and cord blood preptin levels were measured with ELISA. The relationships between maternal serum and cord blood preptin levels, anthropometric and metabolic parameters were also assessed. RESULT: Maternal serum and cord blood preptin levels were found higher in patients with GDM compared with control pregnant women. Preptin concentration in maternal serum was positively correlated with maternal age, fasting insulin levels, 1-h blood glucose after glucose load and cord preptin concentrations at birth. However, relationships between maternal and/or cord serum preptin and fetal growth parameters at birth were not detected. CONCLUSION: Our results indicate that preptin concentrations increase in maternal serum of women with GDM. Preptin levels may provide a novel approach to identify women with GDM.
Asunto(s)
Glucemia/análisis , Diabetes Gestacional , Sangre Fetal , Fragmentos de Péptidos/sangre , Adulto , Peso al Nacer , Índice de Masa Corporal , Diabetes Gestacional/sangre , Diabetes Gestacional/diagnóstico , Femenino , Feto/metabolismo , Edad Gestacional , Prueba de Tolerancia a la Glucosa , Humanos , Insulina/sangre , Factor II del Crecimiento Similar a la Insulina , Edad Materna , Embarazo , Factores de RiesgoRESUMEN
We report the cases of two patients with head and neck Merkel cell carcinoma (MCC) who developed local recurrences confirmed by cytopathology. Interphase fluorescent in situ hybridization (FISH) analysis was performed for research purposes using centromeric probes of chromosomes 6 and 8, on cytological slides. Trisomy of chromosome 6 was found in 85% of tumour cells in the first case of MCC and case 2 exhibited trisomy 8 in 77% of tumour cells. In the absence of specific molecular markers, detection of trisomy 6 and/or trisomy 8 could help in identifying MCC. FISH analysis is easily and quickly performed on interphase nuclei obtained through fine needle aspiration and may be extended to the study of other relevant genetic abnormalities.