Fixed-time artificial insemination protocols on brazilian locally adapted breed gilts on ovulatory response and embryo production.

The aim of this study was to use estrus synchronization protocols to favor fixed-time artificial insemination and consequently fixed-time embryo collection, and increase embryo production using eCG, in gits. In a cross over design, nine Piau breed gilts were subjected to 18 days of oral progesterone; P4 group did not receive any further; GnRH group received 25µg of GnRH 104 hours after the final application of P4; and eCG+GnRH group received 1000IU of eCG 24 hours after the final P4 in addition to GnRH for subsequent embryo collection, that was performed six days after first AI, by laparotomy. Artificial insemination was performed after 12 and 24 hours of estrus in P4 group, and 128 and 144 hours in GnRH and eCG+GnRH groups. The number of CL (8.6±3.9; 8.3±2.1; 26.7±15.0) and anovulatory follicles (4.3±3.7; 3.9±3.9; 17.2±9.5) was higher in the eCG+GnRH gilts (P<0.05). However, the use of 1000 IU of eCG reduced (P<0.05) the number of total structures (5.2±3.6; 5.1±3.1; 1.7±2.7), viable embryos (5.0±3.5; 4.8±3.3; 0.4±0.7), freezable embryos (3.6±3.4; 3.3±3.8; 0.1±0.3) and recovery rate (63.7±38.9; 58.6±24.7; 5.38±9.5). P4 and GnRH protocols were effective in the production and recovery of embryos. However, the use of 1000 IU of eCG, 24 hours after P4, was not effective in promoting the production of embryos, although the animals had superovulated.

Synchronization of follicular wave emergence does not improve embryonic yield in superovulated ewes.

The present study aimed to investigate the effects of a combination of progesterone with different doses of E-17ß on following end points: (1) ovarian follicular dynamics and emergence of a new follicular wave, and (2) superovulatory response and embryo yield. In Experiment 1, 28 ewes were randomly divided into four groups (n = 7) to receive either 2.0 mg, 1.0 mg, 0.5 mg or none E-17ß one day after insertion of a progesterone device. The different doses of estradiol similarly delayed the moment of follicular emergence (overall mean = 3.1 ± 1.0 days vs. control group = 0.86 ± 1.0 days; P < 0.01), but the emergence of the new wave showed greater synchronization with the 0.5 mg dosage of E-17ß. In Experiment 2, sixty-two donor ewes received an internal progesterone release device (day -1) for 7 d and 1 d after the insertion of this device (day 0) were allocated randomly to receive 0.5 mg of E-17ß or only the vehicle (control group). Superstimulation was initiated on day 3 with the administration of 133 mg of pFSH in eight decreasing doses. Contrary to expectations, the protocol with the administration of 0.5 mg E-17ß did not improve the percentage of donors with > 2 CL, the number of CL and the production of embryos (P > 0.05). It was concluded that the combination of progesterone and 0.5 mg E-17ß was more efficient in synchronizing the emergence of the new follicular wave, however this approach seems to be unnecessary in ewe's superovulation programs.

Synchronization of follicular wave emergence does not improve embryonic yield in superovulated ewes

Abstract The present study aimed to investigate the effects of a combination of progesterone with different doses of E-17β on following end points: (1) ovarian follicular dynamics and emergence of a new follicular wave, and (2) superovulatory response and embryo yield. In Experiment 1, 28 ewes were randomly divided into four groups (n = 7) to receive either 2.0 mg, 1.0 mg, 0.5 mg or none E-17β one day after insertion of a progesterone device. The different doses of estradiol similarly delayed the moment of follicular emergence (overall mean = 3.1 ± 1.0 days vs. control group = 0.86 ± 1.0 days; P < 0.01), but the emergence of the new wave showed greater synchronization with the 0.5 mg dosage of E-17β. In Experiment 2, sixty-two donor ewes received an internal progesterone release device (day -1) for 7 d and 1 d after the insertion of this device (day 0) were allocated randomly to receive 0.5 mg of E-17β or only the vehicle (control group). Superstimulation was initiated on day 3 with the administration of 133 mg of pFSH in eight decreasing doses. Contrary to expectations, the protocol with the administration of 0.5 mg E-17β did not improve the percentage of donors with > 2 CL, the number of CL and the production of embryos (P > 0.05). It was concluded that the combination of progesterone and 0.5 mg E-17β was more efficient in synchronizing the emergence of the new follicular wave, however this approach seems to be unnecessary in ewe's superovulation programs.

Fixed-time artificial insemination protocols on brazilian locally adapted breed gilts on ovulatory response and embryo production

The aim of this study was to use estrus synchronization protocols to favor fixed-time artificial insemination and consequently fixed-time embryo collection, and increase embryo production using eCG, in gits. In a cross over design, nine Piau breed gilts were subjected to 18 days of oral progesterone; P4 group did not receive any further; GnRH group received 25µg of GnRH 104 hours after the final application of P4; and eCG+GnRH group received 1000IU of eCG 24 hours after the final P4 in addition to GnRH for subsequent embryo collection, that was performed six days after first AI, by laparotomy. Artificial insemination was performed after 12 and 24 hours of estrus in P4 group, and 128 and 144 hours in GnRH and eCG+GnRH groups. The number of CL (8.6±3.9; 8.3±2.1; 26.7±15.0) and anovulatory follicles (4.3±3.7; 3.9±3.9; 17.2±9.5) was higher in the eCG+GnRH gilts (P<0.05). However, the use of 1000 IU of eCG reduced (P<0.05) the number of total structures (5.2±3.6; 5.1±3.1; 1.7±2.7), viable embryos (5.0±3.5; 4.8±3.3; 0.4±0.7), freezable embryos (3.6±3.4; 3.3±3.8; 0.1±0.3) and recovery rate (63.7±38.9; 58.6±24.7; 5.38±9.5). P4 and GnRH protocols were effective in the production and recovery of embryos. However, the use of 1000 IU of eCG, 24 hours after P4, was not effective in promoting the production of embryos, although the animals had superovulated.(AU)

Synchronization of follicular wave emergence does not improve embryonic yield in superovulated ewes

The present study aimed to investigate the effects of a combination of progesterone with different doses of E-17β on following end points: (1) ovarian follicular dynamics and emergence of a new follicular wave, and (2) superovulatory response and embryo yield. In Experiment 1, 28 ewes were randomly divided into four groups (n = 7) to receive either 2.0 mg, 1.0 mg, 0.5 mg or none E-17β one day after insertion of a progesterone device. The different doses of estradiol similarly delayed the moment of follicular emergence (overall mean = 3.1 ± 1.0 days vs. control group = 0.86 ± 1.0 days; P < 0.01), but the emergence of the new wave showed greater synchronization with the 0.5 mg dosage of E-17β. In Experiment 2, sixty-two donor ewes received an internal progesterone release device (day -1) for 7 d and 1 d after the insertion of this device (day 0) were allocated randomly to receive 0.5 mg of E-17β or only the vehicle (control group). Superstimulation was initiated on day 3 with the administration of 133 mg of pFSH in eight decreasing doses. Contrary to expectations, the protocol with the administration of 0.5 mg E-17β did not improve the percentage of donors with > 2 CL, the number of CL and the production of embryos (P > 0.05). It was concluded that the combination of progesterone and 0.5 mg E-17β was more efficient in synchronizing the emergence of the new follicular wave, however this approach seems to be unnecessary in ewes superovulation programs.(AU)

Concentração de proteínas do plasma seminal e sua relação com a criopreservação do sêmen ovino em diferentes diluentes / Protein concentration of seminal plasma and their relation with cryopreservation of ram semen using differents extenders

O objetivo deste trabalho foi avaliar a relação entre a concentração de proteínas no plasma seminal ovino com parâmetros de qualidade do sêmen criopreservado em diluentes TRIS e em Água de Coco em Pó (ACP102c). Um total de 12 coletas/animal em ovinos Santa Inês (n=2) e Dorper (n=2) foram realizadas para obtenção do plasma seminal e para criopreservação nos diluentes de trabalho. Foi utilizado o método de Bradford para determinação da concentração de proteína. Para avaliação do sêmen fresco e criopreservado foram utilizados os testes de viabilidade por eosina-nigrosina e teste hiposmótico (HOST), além dos parâmetros de motilidade em sistema de análise de sêmen auxiliada por computador (CASA). Baseado no valor médio da concentração de proteína, os ejaculados foram agrupados em alta (AP) e baixa (BP) concentração proteica. Correlações significativamente positivas foram encontradas entre a concentração de proteína com os parâmetros motilidade progressiva (PROG) e congelabilidade (CONGEL), e negativa para deslocamento lateral de cabeça (ALH) nas amostras criopreservadas em ambos os diluentes, além de correlação positiva para motilidade total (MOT) em TRIS. Sêmen de ejaculados do grupo AP criopreservados em TRIS apresentaram MOT, PROG e CONGEL significativamente superior ao grupo BP e inferiores para ALH. Quando criopreservado em ACP102c, sêmen do grupo AP foi superior ao BP para PROG e CONGEL. Variações na qualidade do sêmen criopreservado entre ejaculados podem estar associados à concentração de proteínas do plasma seminal, e estas podem atuar na manutenção da progressividade de espermatozoides ovinos criopreservados em diluentes TRIS e ACP102c.(AU)

The objective of this study was to evaluate the relation between the protein concentration in ram seminal plasma and quality parameters of the semen cryopreserved in TRIS and Coconut Water Powder (ACP102c) extenders. A total of 12 semen collections/animal of Santa Ines (n=2) and Dorper (n=2) rams were performed to obtain seminal plasma and semen cryopreservation in work extenders. The protein content was determined using the Bradford ́s method. The eosin-nigrosin and hyposmotic (HOST) viable tests and motility parameters obtained by computer assisted semen analysis were evaluated for fresh and cryopreserved semen. The ejaculates were grouped in high (AP) and low (BP) protein content, based on the mean value of protein concentration. Significantly positive correlations were found between the protein content and progressive motility (PROG), freezability (CONGEL) and negative for lateral head displacement (ALH) in the semen cryopreserved in both extenders. Therefore, positive correlation was found for total motility (MOT) in TRIS. Semen samples from AP group cryopreserved in TRIS extender were significantly better than BP group for MOT, PROG and CONGEL, and inferior for ALH. When criopreserved in ACP102c extender, the AP group were better than BP group for PROG and CONGEL. Variations in the quality of cryopreserved semen from different ejaculates may be associated with the concentration of seminal plasma proteins and may act to maintain the progressiveness of ram sperm cryopreserved in TRIS and ACP102c extenders.(AU)

Concentração de proteínas do plasma seminal e sua relação com a criopreservação do sêmen ovino em diferentes diluentes / Protein concentration of seminal plasma and their relation with cryopreservation of ram semen using differents extenders

O objetivo deste trabalho foi avaliar a relação entre a concentração de proteínas no plasma seminal ovino com parâmetros de qualidade do sêmen criopreservado em diluentes TRIS e em Água de Coco em Pó (ACP102c). Um total de 12 coletas/animal em ovinos Santa Inês (n=2) e Dorper (n=2) foram realizadas para obtenção do plasma seminal e para criopreservação nos diluentes de trabalho. Foi utilizado o método de Bradford para determinação da concentração de proteína. Para avaliação do sêmen fresco e criopreservado foram utilizados os testes de viabilidade por eosina-nigrosina e teste hiposmótico (HOST), além dos parâmetros de motilidade em sistema de análise de sêmen auxiliada por computador (CASA). Baseado no valor médio da concentração de proteína, os ejaculados foram agrupados em alta (AP) e baixa (BP) concentração proteica. Correlações significativamente positivas foram encontradas entre a concentração de proteína com os parâmetros motilidade progressiva (PROG) e congelabilidade (CONGEL), e negativa para deslocamento lateral de cabeça (ALH) nas amostras criopreservadas em ambos os diluentes, além de correlação positiva para motilidade total (MOT) em TRIS. Sêmen de ejaculados do grupo AP criopreservados em TRIS apresentaram MOT, PROG e CONGEL significativamente superior ao grupo BP e inferiores para ALH. Quando criopreservado em ACP102c, sêmen do grupo AP foi superior ao BP para PROG e CONGEL. Variações na qualidade do sêmen criopreservado entre ejaculados podem estar associados à concentração de proteínas do plasma seminal, e estas podem atuar na manutenção da progressividade de espermatozoides ovinos criopreservados em diluentes TRIS e ACP102c.

The objective of this study was to evaluate the relation between the protein concentration in ram seminal plasma and quality parameters of the semen cryopreserved in TRIS and Coconut Water Powder (ACP102c) extenders. A total of 12 semen collections/animal of Santa Ines (n=2) and Dorper (n=2) rams were performed to obtain seminal plasma and semen cryopreservation in work extenders. The protein content was determined using the Bradford ́s method. The eosin-nigrosin and hyposmotic (HOST) viable tests and motility parameters obtained by computer assisted semen analysis were evaluated for fresh and cryopreserved semen. The ejaculates were grouped in high (AP) and low (BP) protein content, based on the mean value of protein concentration. Significantly positive correlations were found between the protein content and progressive motility (PROG), freezability (CONGEL) and negative for lateral head displacement (ALH) in the semen cryopreserved in both extenders. Therefore, positive correlation was found for total motility (MOT) in TRIS. Semen samples from AP group cryopreserved in TRIS extender were significantly better than BP group for MOT, PROG and CONGEL, and inferior for ALH. When criopreserved in ACP102c extender, the AP group were better than BP group for PROG and CONGEL. Variations in the quality of cryopreserved semen from different ejaculates may be associated with the concentration of seminal plasma proteins and may act to maintain the progressiveness of ram sperm cryopreserved in TRIS and ACP102c extenders.

Perfil proteico do plasma seminal ovino e sua relação com a criopreservação nos diluentes TRIS e Água de Coco em Pó / Protein profile of ram seminal plasm and its relation relationship with cryopreservation in TRIS and Powdered Coconut Water extender

O objetivo deste trabalho foi avaliar a relação entre a concentração de proteínas no plasma seminal ovino com parâmetros de qualidade do sêmen criopreservado em diluentes TRIS e em Água de Coco em Pó (ACP102c). Um total de 12 colheitas/animal em ovinos Santa Inês (n=2) e Dorper (n=2) foram realizadas, para obtenção do plasma seminal e para criopreservação nos diluentes testados. Foi utilizado o método de Bradford para determinação da concentração de proteína. Para avaliação do sêmen fresco e criopreservado foram utilizados os testes de viabilidade por eosina-nigrosina e teste hiposmótico (HOST), além dos parâmetros de motilidade em sistema de análise de sêmen auxiliada por computador (CASA). Baseado no valor médio da concentração de proteína, os ejaculados foram agrupados em alta (AP) e baixa (BP) concentração proteica. Correlações significativamente positivas foram encontradas entre a concentração de proteína com os parâmetros motilidade progressiva (PROG) e congelabilidade (CONGEL) e negativa para deslocamento lateral de cabeça (ALH) nas amostras criopreservadas em ambos diluentes, além de correlação positiva para motilidade total (MOT) em TRIS. Sêmen de ejaculados do grupo AP criopreservados em TRIS apresentaram MOT, PROG e CONGEL significativamente superiores ao grupo BP e inferiores para ALH. Quando criopreservado em ACP102c, sêmen do grupo AP foi superior ao BP para PROG e CONGEL. Variações na qualidade do sêmen criopreservado entre ejaculados podem estar associados à concentração de proteínas do plasma seminal, e estas podem atuar na manutenção da progressividade de espermatozoides ovinos criopreservados em diluentes TRIS e ACP102c.(AU)

The objective of this study was to evaluate the relation between the protein concentration in ram seminal plasma and quality parameters of the semen cryopreserved in TRIS and Powdered Coconut Water (ACP102c) extenders. A total of 12 semen collections/animal of Santa Ines (n=2) and Dorper (n=2) rams were performed to obtain seminal plasma and semen cryopreservation in work extenders. The protein content was determined using the Bradford's method. The eosin-nigrosine and hyposmotic (HOST) viable tests and motility parameters obtained by computer assisted semen analysis were evaluated for fresh and cryopreserved semen. The ejaculates were grouped in high (AP) and low (BP) protein content, based on the mean value of protein concentration. Significantly positive correlations were found between the protein content and progressive motility (PROG), freezability (CONGEL) and negative for lateral head displacement (ALH) in the semen cryopreserved in both extenders. Therefore, positive correlation was found for total motility (MOT) in TRIS. Semen samples from AP group cryopreserved in TRIS extender were significantly better than BP group for MOT, PROG and CONGEL, and inferior for ALH. When cryopreserved in ACP102c, the AP group were better than BP group for PROG and CONGEL. Variations in the quality of cryopreserved semen from different ejaculates may be associated with the concentration of seminal plasma proteins and may act to maintain the progressiveness of ram sperm cryopreserved in TRIS and ACP102c extenders.(AU)

Perfil proteico do plasma seminal ovino e sua relação com a criopreservação nos diluentes TRIS e Água de Coco em Pó / Protein profile of ram seminal plasm and its relation relationship with cryopreservation in TRIS and Powdered Coconut Water extender

O objetivo deste trabalho foi avaliar a relação entre a concentração de proteínas no plasma seminal ovino com parâmetros de qualidade do sêmen criopreservado em diluentes TRIS e em Água de Coco em Pó (ACP102c). Um total de 12 colheitas/animal em ovinos Santa Inês (n=2) e Dorper (n=2) foram realizadas, para obtenção do plasma seminal e para criopreservação nos diluentes testados. Foi utilizado o método de Bradford para determinação da concentração de proteína. Para avaliação do sêmen fresco e criopreservado foram utilizados os testes de viabilidade por eosina-nigrosina e teste hiposmótico (HOST), além dos parâmetros de motilidade em sistema de análise de sêmen auxiliada por computador (CASA). Baseado no valor médio da concentração de proteína, os ejaculados foram agrupados em alta (AP) e baixa (BP) concentração proteica. Correlações significativamente positivas foram encontradas entre a concentração de proteína com os parâmetros motilidade progressiva (PROG) e congelabilidade (CONGEL) e negativa para deslocamento lateral de cabeça (ALH) nas amostras criopreservadas em ambos diluentes, além de correlação positiva para motilidade total (MOT) em TRIS. Sêmen de ejaculados do grupo AP criopreservados em TRIS apresentaram MOT, PROG e CONGEL significativamente superiores ao grupo BP e inferiores para ALH. Quando criopreservado em ACP102c, sêmen do grupo AP foi superior ao BP para PROG e CONGEL. Variações na qualidade do sêmen criopreservado entre ejaculados podem estar associados à concentração de proteínas do plasma seminal, e estas podem atuar na manutenção da progressividade de espermatozoides ovinos criopreservados em diluentes TRIS e ACP102c.

The objective of this study was to evaluate the relation between the protein concentration in ram seminal plasma and quality parameters of the semen cryopreserved in TRIS and Powdered Coconut Water (ACP102c) extenders. A total of 12 semen collections/animal of Santa Ines (n=2) and Dorper (n=2) rams were performed to obtain seminal plasma and semen cryopreservation in work extenders. The protein content was determined using the Bradford's method. The eosin-nigrosine and hyposmotic (HOST) viable tests and motility parameters obtained by computer assisted semen analysis were evaluated for fresh and cryopreserved semen. The ejaculates were grouped in high (AP) and low (BP) protein content, based on the mean value of protein concentration. Significantly positive correlations were found between the protein content and progressive motility (PROG), freezability (CONGEL) and negative for lateral head displacement (ALH) in the semen cryopreserved in both extenders. Therefore, positive correlation was found for total motility (MOT) in TRIS. Semen samples from AP group cryopreserved in TRIS extender were significantly better than BP group for MOT, PROG and CONGEL, and inferior for ALH. When cryopreserved in ACP102c, the AP group were better than BP group for PROG and CONGEL. Variations in the quality of cryopreserved semen from different ejaculates may be associated with the concentration of seminal plasma proteins and may act to maintain the progressiveness of ram sperm cryopreserved in TRIS and ACP102c extenders.

Use of FSH: LH as an alternative to stimulate follicular growth in sheep estrus synchronization / Utilização de FSH: LH como alternativa para estimular o crescimento folicular na sincronização do estro ovino

Background: Estrus synchronization is extensively applied in reproductive management of sheep world-wide. The use ofequine chorionic gonadotropin (eCG) in estrus synchronization protocols in sheep is well established. However, development of anti-eCG antibodies after repeated synchronizations results in poor synchronization and, eventually, in reducedfertility and lambing rates, especially when fixed time AI is applied. Works using FSH in estrus synchronization have beenscarce to date. So, the objective of the current study was to evaluate the effect of different follicular growth promoters(FSH:LH and eCG) on estrus synchronization and ovulation in sheep.Materials, Methods & Results: Forty four ewes were submitted to estrus synchronization with medroxyprogesterone acetate (MAP) intravaginal devices for 12 days and a single i.m. treatment of either eCG (Group 300eCG, 300 UI, n = 11) orFSH:LH (Group 20FSH:LH, 20 UI, n = 11; Group 40FSH:LH, 40 UI, n = 11; Group G20/20FSH:LH, 40 UI divided intotwo doses injected 12 h before removal device time and at the exact removal time, n = 11) at the time of device removal.At the sponge removal, ewes were exposed to a teaser and estrus was monitored at 4-h intervals until all females showedthe clinical estrus. After 12 hours of estrus onset, ultrassonografic exams were performed to record ovarian response.Besides, blood samples were collected for progesterone (P4) measurements. Statistical analyses were performed usingANOVA and means were compared by Duncan test. Kurskal-Wallis test was used for corpus luteum and progesteronecomparison, considering a 5% significance level. There was no difference (P > 0.05) on the time of estrus (46.3 ± 11.8;52.4 ± 10.7; 53.0 ± 7.2; 51.8 ± 7.1) or ovulation (80.4 ± 17.3; 86.9 ± 11.0; 95.3 ± 6.1; 85.0 ± 5.7) among the 300eCG,20FSH:LH, 40FSH:LH, 20/20FSH:LH groups, respectively. It was noticed, however, that there has been a variation in the...

Use of FSH: LH as an alternative to stimulate follicular growth in sheep estrus synchronization / Utilização de FSH: LH como alternativa para estimular o crescimento folicular na sincronização do estro ovino

Background: Estrus synchronization is extensively applied in reproductive management of sheep world-wide. The use ofequine chorionic gonadotropin (eCG) in estrus synchronization protocols in sheep is well established. However, development of anti-eCG antibodies after repeated synchronizations results in poor synchronization and, eventually, in reducedfertility and lambing rates, especially when fixed time AI is applied. Works using FSH in estrus synchronization have beenscarce to date. So, the objective of the current study was to evaluate the effect of different follicular growth promoters(FSH:LH and eCG) on estrus synchronization and ovulation in sheep.Materials, Methods & Results: Forty four ewes were submitted to estrus synchronization with medroxyprogesterone acetate (MAP) intravaginal devices for 12 days and a single i.m. treatment of either eCG (Group 300eCG, 300 UI, n = 11) orFSH:LH (Group 20FSH:LH, 20 UI, n = 11; Group 40FSH:LH, 40 UI, n = 11; Group G20/20FSH:LH, 40 UI divided intotwo doses injected 12 h before removal device time and at the exact removal time, n = 11) at the time of device removal.At the sponge removal, ewes were exposed to a teaser and estrus was monitored at 4-h intervals until all females showedthe clinical estrus. After 12 hours of estrus onset, ultrassonografic exams were performed to record ovarian response.Besides, blood samples were collected for progesterone (P4) measurements. Statistical analyses were performed usingANOVA and means were compared by Duncan test. Kurskal-Wallis test was used for corpus luteum and progesteronecomparison, considering a 5% significance level. There was no difference (P > 0.05) on the time of estrus (46.3 ± 11.8;52.4 ± 10.7; 53.0 ± 7.2; 51.8 ± 7.1) or ovulation (80.4 ± 17.3; 86.9 ± 11.0; 95.3 ± 6.1; 85.0 ± 5.7) among the 300eCG,20FSH:LH, 40FSH:LH, 20/20FSH:LH groups, respectively. It was noticed, however, that there has been a variation in the...(AU)

Criopreservação do sêmen ovino em meio diluente à base de água de coco em pó (ACP-102c) / Cryopreservation of ram semen in powdered coconut water (ACP-102c) based extender

The aim of this study was to evaluate the ACP-102c extender in the cryopreservation of ram semen compared to tris-glucose-egg yolk (TRIS) extender and fresh semen. Forty-eight ejaculates were collected from four rams and two aliquots per ejaculate were taken for dilution and cryopreservation in ACP-102c or TRIS and a third aliquot used for the fresh semen analysis. Either the fresh semen and cryopreserved in both extenders were evaluated for viability, integrity of plasma and acrosomal membrane, hypoosmotic swelling test, DNA fragmentation and sperm motility. The extenders did not differ for sperm viability, acrosome and plasma membrane integrity, DNA fragmentation and quantitative and qualitative parameters of sperm velocity after thawing, but differed in hypoosmotic swelling test, total and progressive motility and lateral extent of the head as well as in all motility parameters evaluated (except linearity and straightness) after two hours of incubation at 37 oC. There was variability among rams in total and progressive motility of semen cryopreserved in ACP-102c after thawing. The ACP-102c extender showed less protection in the cryopreservation of ram sperm when compared to TRIS, but the improvement in its formulation and freezing protocols may make it an alternative to freezing ram semen.

O objetivo deste trabalho foi avaliar o diluente ACP-102c na criopreservação do sêmen ovino em comparação com o diluidor tris-glicose-gema (TRIS) e o sêmen fresco. Foram coletados 48 ejaculados de quatro ovinos, sendo tomadas duas alíquotas por ejaculado para diluição e criopreservação em ACP-102c ou TRIS e uma terceira alíquota utilizada para análise do sêmen fresco. O sêmen fresco e o criopreservado em ambos os diluidores foram avaliados para viabilidade, integridade de membrana plasmática e acrossomal, teste hiposmótico, fragmentação do DNA e de motilidade espermática. Após descongelamento, ambos os diluidores não diferiram para viabilidade espermática, integridade de membrana plasmática e acrossomal, fragmentação de DNA e nas variáveis quantitativas e qualitativas de velocidade espermática, mas diferiram no teste hiposmótico, motilidade total e progressiva e amplitude lateral da cabeça, bem como em todas as variáveis de motilidade avaliadas, exceto linearidade e progressividade, após duas horas de incubação à 37 oC. Houve variabilidade entre reprodutores na motilidade total e progressiva do sêmen criopreservado em ACP-102c após descongelamento. O diluidor ACP-102c conferiu menor proteção aos espermatozoides ovinos contra danos do congelamento quando comparado ao TRIS, mas o aprimoramento de sua formulação e protocolos mais adequados de congelação poderão torná-lo uma alternativa na congelação do sêmenovino.

Criopreservação do sêmen ovino em meio diluente à base de água de coco em pó (ACP-102c) / Cryopreservation of ram semen in powdered coconut water (ACP-102c) based extender

The aim of this study was to evaluate the ACP-102c extender in the cryopreservation of ram semen compared to tris-glucose-egg yolk (TRIS) extender and fresh semen. Forty-eight ejaculates were collected from four rams and two aliquots per ejaculate were taken for dilution and cryopreservation in ACP-102c or TRIS and a third aliquot used for the fresh semen analysis. Either the fresh semen and cryopreserved in both extenders were evaluated for viability, integrity of plasma and acrosomal membrane, hypoosmotic swelling test, DNA fragmentation and sperm motility. The extenders did not differ for sperm viability, acrosome and plasma membrane integrity, DNA fragmentation and quantitative and qualitative parameters of sperm velocity after thawing, but differed in hypoosmotic swelling test, total and progressive motility and lateral extent of the head as well as in all motility parameters evaluated (except linearity and straightness) after two hours of incubation at 37 oC. There was variability among rams in total and progressive motility of semen cryopreserved in ACP-102c after thawing. The ACP-102c extender showed less protection in the cryopreservation of ram sperm when compared to TRIS, but the improvement in its formulation and freezing protocols may make it an alternative to freezing ram semen.(AU)

O objetivo deste trabalho foi avaliar o diluente ACP-102c na criopreservação do sêmen ovino em comparação com o diluidor tris-glicose-gema (TRIS) e o sêmen fresco. Foram coletados 48 ejaculados de quatro ovinos, sendo tomadas duas alíquotas por ejaculado para diluição e criopreservação em ACP-102c ou TRIS e uma terceira alíquota utilizada para análise do sêmen fresco. O sêmen fresco e o criopreservado em ambos os diluidores foram avaliados para viabilidade, integridade de membrana plasmática e acrossomal, teste hiposmótico, fragmentação do DNA e de motilidade espermática. Após descongelamento, ambos os diluidores não diferiram para viabilidade espermática, integridade de membrana plasmática e acrossomal, fragmentação de DNA e nas variáveis quantitativas e qualitativas de velocidade espermática, mas diferiram no teste hiposmótico, motilidade total e progressiva e amplitude lateral da cabeça, bem como em todas as variáveis de motilidade avaliadas, exceto linearidade e progressividade, após duas horas de incubação à 37 oC. Houve variabilidade entre reprodutores na motilidade total e progressiva do sêmen criopreservado em ACP-102c após descongelamento. O diluidor ACP-102c conferiu menor proteção aos espermatozoides ovinos contra danos do congelamento quando comparado ao TRIS, mas o aprimoramento de sua formulação e protocolos mais adequados de congelação poderão torná-lo uma alternativa na congelação do sêmenovino.(AU)

Criopreservação do sêmen ovino em meio diluente à base de água de coco em pó (ACP-102c)

The aim of this study was to evaluate the ACP-102c extender in the cryopreservation of ram semen compared to tris-glucose-egg yolk (TRIS) extender and fresh semen. Forty-eight ejaculates were collected from four rams and two aliquots per ejaculate were taken for dilution and cryopreservation in ACP-102c or TRIS and a third aliquot used for the fresh semen analysis. Either the fresh semen and cryopreserved in both extenders were evaluated for viability, integrity of plasma and acrosomal membrane, hypoosmotic swelling test, DNA fragmentation and sperm motility. The extenders did not differ for sperm viability, acrosome and plasma membrane integrity, DNA fragmentation and quantitative and qualitative parameters of sperm velocity after thawing, but differed in hypoosmotic swelling test, total and progressive motility and lateral extent of the head as well as in all motility parameters evaluated (except linearity and straightness) after two hours of incubation at 37 ºC. There was variability among rams in total and progressive motility of semen cryopreserved in ACP-102c after thawing. The ACP-102c extender showed less protection in the cryopreservation of ram sperm when compared to TRIS, but the improvement in its formulation and freezing protocols may make it an alternative to freezing ram semen.

O objetivo deste trabalho foi avaliar o diluente ACP-102c na criopreservação do sêmen ovino em comparação com o diluidor tris-glicose-gema (TRIS) e o sêmen fresco. Foram coletados 48 ejaculados de quatro ovinos, sendo tomadas duas alíquotas por ejaculado para diluição e criopreservação em ACP-102c ou TRIS e uma terceira alíquota utilizada para análise do sêmen fresco. O sêmen fresco e o criopreservado em ambos os diluidores foram avaliados para viabilidade, integridade de membrana plasmática e acrossomal, teste hiposmótico, fragmentação do DNA e de motilidade espermática. Após descongelamento, ambos os diluidores não diferiram para viabilidade espermática, integridade de membrana plasmática e acrossomal, fragmentação de DNA e nas variáveis quantitativas e qualitativas de velocidade espermática, mas diferiram no teste hiposmótico, motilidade total e progressiva e amplitude lateral da cabeça, bem como em todas as variáveis de motilidade avaliadas, exceto linearidade e progressividade, após duas horas de incubação à 37 ºC. Houve variabilidade entre reprodutores na motilidade total e progressiva do sêmen criopreservado em ACP-102c após descongelamento. O diluidor ACP-102c conferiu menor proteção aos espermatozoides ovinos contra danos do congelamento quando comparado ao TRIS, mas o aprimoramento de sua formulação e protocolos mais adequados de congelação poderão torná-lo uma alternativa na congelação do sêmen ovino.