RESUMEN
Methodical approaches to detecting cultures producing streptothricins at the early stages of screening new antibiotics were developed. The approaches are based on chromatographic and electrophoretic mobility of streptothricins and the products of their hydrolysis in the extracts from agar cultures of actinomycetes. Application of the method for screening new antibiotics is illustrated with an example. Nine strains of actinomyces with broad antibacterial spectra isolated from soil samples were studied and 6 of them belonging to 4 species were shown to produce streptothricins in agar cultures. The new streptothricin-producing culture S. roseolilacinus was isolated.
Asunto(s)
Estreptotricinas/análisis , Hidrólisis , Streptomyces/aislamiento & purificación , Factores de TiempoRESUMEN
By a combination of chemical and spectroscopic (1H and 13C NMR) studies the structure of a glycopeptide antibiotic eremomycin has been elucidated. It is closely related to vancomycin, but differs in sugar and chlorine content. The eremomycin aglycone contains monodechlorovancomycinic acid; the only chlorine atom is situated in the second amino acid after the N-terminal amino acid residue of the peptide. The sugar part is composed of glucose and two residues of an amino sugar shown to be 2,3,6-trideoxy-3-amino-C-3-methyl-L-arabino-hexopyranose (4-epi-vancosamine). One of the amino sugar residues is a component of the disaccharide 2-O-(alpha-L-4-epi-vancosaminyl)-beta-D-glucopyranose, attached to a triphenyl ether moiety; the position of another one is at the serine oxygen in the C-terminal region of the aglycone.
Asunto(s)
Antibacterianos , Péptidos Catiónicos Antimicrobianos , Fenómenos Químicos , Química , Glicopéptidos , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Péptidos , Espectrofotometría Infrarroja , Espectrofotometría UltravioletaRESUMEN
Eremomycin is a novel antibacterial antibiotic. It was isolated at the Institute of New Antibiotics, the USSR Academy of Medical Sciences from the culture fluid of actinomycete INA-238. By its physico-chemical and biological properties the antibiotic was classified as belonging to the group of polycyclic glycopeptides. Chemical structure of eremomycin was asserted and it was shown to be a new representative of the group close by its structure to vancomycin and differing from it by the carbohydrate composition and structure of tri-phenoxytriaminotricarboxylic acid. By its anti-bacterial spectrum eremomycin was found to be close to ristomycin and vancomycin. Still, its activity was 2-10 times higher. The antibiotic was several times less toxic than vancomycin. Unlike vancomycin and ristomycin, the novel antibiotic induced no tissue necrosis after its intramuscular administration. The chemotherapeutic indices of eremomycin in treatment of staphylococcal and streptococcal sepsis in albino mice exceeded 10 times those of vancomycin. At present eremomycin is under clinical trials.
Asunto(s)
Antibacterianos , Glicopéptidos/análisis , Animales , Fenómenos Químicos , Química , Evaluación Preclínica de Medicamentos , Glicopéptidos/clasificación , Glicopéptidos/uso terapéutico , Ratones , Infecciones Estafilocócicas/tratamiento farmacológico , Infecciones Estreptocócicas/tratamiento farmacológico , U.R.S.S.RESUMEN
A three component system for separating a mixture of carminomycin, carminomycinone and 13-dihydrocarminomycinone by HPLC was developed. Spherisorb ODS Column, 4.6 X 250 mm, the particle size of 10 micron was used. The impact of the mobile phase composition, temperature during chromatography and buffer solution pH on the capacity factors K' for every of the above compounds was studied. For determining purity of carminomycin dosage forms the procedure with an external standard was applied. The procedure provides routine quantitative assay of carminomycin hydrochloride dosage forms.
Asunto(s)
Carubicina/análisis , Daunorrubicina/análogos & derivados , Tampones (Química) , Carubicina/análogos & derivados , Carubicina/aislamiento & purificación , Cromatografía Líquida de Alta Presión/instrumentación , Cromatografía Líquida de Alta Presión/métodos , Concentración de Iones de HidrógenoRESUMEN
A antitumor antibiotic belonging to the group of polypeptide antibiotics containing chromophore was isolated from the culture of Actinomadura recticatena Terekhova, Preobrazhenskaya et Galatenko, 1984, strain 308. Biosynthesis, isolation, physicochemical and biological properties of the antibiotic are described. The results of elemental analysis, the melting point, optical properties, UV, IR and NMR spectra and the data on acid hydrolysis showed that antibiotic 308 was most closely related to antibiotic BBM-928 A.
Asunto(s)
Actinomycetales/metabolismo , Antibióticos Antineoplásicos/biosíntesis , Biosíntesis de Péptidos , Antibióticos Antineoplásicos/análisis , Antibióticos Antineoplásicos/aislamiento & purificación , Fenómenos Químicos , Química Física , Espectroscopía de Resonancia Magnética , Péptidos/análisis , Péptidos/aislamiento & purificación , Quinolinas/análisis , Quinolinas/biosíntesis , Quinolinas/aislamiento & purificación , Espectrofotometría Infrarroja , Espectrofotometría UltravioletaRESUMEN
Eremomycin, a novel antibiotic belonging to the group of cyclic glycopeptides is produced by an actinomycete designated INA-238. Biosynthesis, isolation, physico-chemical and biological properties of the antibiotic are described. Eremomycin is shown to be close to vancomycin. However, by its chromatographic and electrophoretic mobility, presence of chlorine in the antibiotic molecule and optic characteristics eremomycin differs from vancomycin. Eremomycin is less toxic than vancomycin whereas its chemotherapeutic activity in treatment of infections caused by gram-positive organisms is higher.
Asunto(s)
Antibacterianos/análisis , Animales , Antibacterianos/farmacología , Antibacterianos/toxicidad , Fenómenos Químicos , Química Física , Fermentación , Ratones , Pruebas de Sensibilidad Microbiana , Peso Molecular , Relación Estructura-Actividad , Vancomicina/análisis , Vancomicina/farmacología , Vancomicina/toxicidadRESUMEN
Octamycin, an original polyenic antibiotic was developed. The antibiotic is of an amphoteric character. The number and nature of the functional groups were determined by potentiometric titration. It was shown that the antibiotic contained the neutral sugar galactose. The equivalent weight of the antibiotic (990) was measured by potentiometric titration and the content of the sugar in the molecule. With high performance liquid chromatography it was demonstrated that the antibiotic consisted of 2 components at a ratio of 1:2. The molecular weights of the components were evaluated with mass spectrometry.
Asunto(s)
Antibacterianos/farmacología , Antibacterianos/análisis , Fenómenos Químicos , Química Física , Cromatografía en Gel , Hidrólisis , Espectrometría de Masas , Polienos/análisis , Polienos/farmacología , Potenciometría , Espectrofotometría UltravioletaRESUMEN
In screening new antibiotics, varigomycin produced by a new fungal species Streptomyces variegatus Sveshnikova et Timuk was isolated. By physicochemical properties varigomycin belongs to carbonyl conjugated pentaens. It is a new representative of this antibiotic group. Varigomycin differs from the known representatives of this group by the presence of glucose, sugar in its molecule.
Asunto(s)
Antibacterianos/biosíntesis , Acilación , Antibacterianos/análisis , Antibacterianos/aislamiento & purificación , Fenómenos Químicos , Química Física , Cromatografía en Capa Delgada , Hidrólisis , Espectrometría de Masas , Polienos/análisis , Polienos/biosíntesis , Polienos/aislamiento & purificación , Espectrofotometría Infrarroja , Espectrofotometría Ultravioleta , Streptomyces/metabolismoRESUMEN
To identify the structure of virenomycin, a new antitumor antibiotic consisting of components V and M, its acetyl and permethyl derivatives, as well as products of acid methanolysis and their derivatives were obtained. The IR-, NMR- and mass-spectra of the above compounds are presented. Based on an analysis of the spectral data the structure of virenomycin is suggested.
Asunto(s)
Antibióticos Antineoplásicos , Fenómenos Químicos , Química , Espectroscopía de Resonancia Magnética , Espectrometría de Masas/métodos , Metilglicósidos , Espectrofotometría InfrarrojaRESUMEN
Streptomycete 1401 inhibiting the growth of gram-positive organisms was isolated from a soil sample in the screening of new antibiotics. By its cultural and morphological properties the culture was classified as Str. corchorusii. An antibiotic complex was isolated from the mycelium of the culture. The main component of the complex was shown to belong to the antibiotics of the siomycin group.
Asunto(s)
Antibacterianos , Antibióticos Antineoplásicos/biosíntesis , Streptomyces/metabolismo , Antibióticos Antineoplásicos/análisis , Antibióticos Antineoplásicos/aislamiento & purificación , Fenómenos Químicos , Química Física , Cromatografía en Capa Delgada , Medios de Cultivo/metabolismo , Biosíntesis de Péptidos , Péptidos/análisis , Péptidos/aislamiento & purificación , Espectrofotometría Infrarroja , Streptomyces/clasificaciónRESUMEN
A total of 160 fungal cultures belonging to Trichoderma were isolated from soil samples collected in various regions of the USSR. The cultures were tested for production of antibiotics of the cyclosporin group, having a narrow antifungal spectrum. It was found that 7 cultures produced substances having a selective inhibitory effect on Asp. niger and no activity against gram-positive and gram-negative organisms. An antibiotic substance close by its physicochemical properties to cyclosporins was isolated from T. viride 122.
Asunto(s)
Antibacterianos/aislamiento & purificación , Ciclosporinas/aislamiento & purificación , Antibacterianos/farmacología , Ciclosporinas/farmacología , Pruebas de Sensibilidad Microbiana , Microbiología del Suelo , Trichoderma/metabolismo , U.R.S.S.RESUMEN
Separation of the main components of the nebramycin complex of apramycin, kanamycin B and tobramycin was achieved in the form of their 2,4-dinitrophenyl derivatives. A chromatograph SR 8000 of 'Spectra-Physics" and a column 'Sperisorb C6" (4.6 X 250 mm) with the granule size of 5 micrometers were used in the study with the mobile phase of acetone-trisbuffer, pH 7 in a ratio of 65 to 35, the flow rate of 1 ml/min, a temperature of 35 degrees C and detection at lambda 350 nm. Quantitative determination of the tobramycin purity level was performed with an equation developed for the given conditions. The purity levels of the reference tobramycin base and its sulfate were estimated. The results of the estimation corresponded to the data of biological titration.
Asunto(s)
Antibacterianos/análisis , Cromatografía Líquida de Alta Presión/métodos , Tobramicina/análisis , 2,4-Dinitrofenol , Cromatografía Líquida de Alta Presión/instrumentación , Dinitrofenoles/análisis , Tobramicina/análogos & derivados , Tobramicina/aislamiento & purificaciónRESUMEN
An antibacterial antibiotic complex consisting of 2 components designated as 2562 A and 2562 B is produced by Streptomyces griseovarabilis. The antibiotic was isolated from the mycelium and purified chromatographically on a column with aqueous silicic acid. The study of the components showed that component 2562 A was chlorbiocin, while component 2562 B differed from the known antibiotics of this group. Physicochemical assays demonstrated that component 2562 B differed from chlorbiocin by the absence of the methyl group in pyrrol, which is probably attached to sugar at beta-position. It was found that component 2562 B is a new representative of the antibiotic cumero-glycoside group.
Asunto(s)
Antibacterianos/aislamiento & purificación , Antibacterianos/análisis , Fenómenos Químicos , Química Física , Cristalización , Espectroscopía de Resonancia por Spin del Electrón , Hidrólisis , Novobiocina/análogos & derivados , Espectrofotometría Infrarroja , Espectrofotometría Ultravioleta , Streptomyces/metabolismoRESUMEN
Carminomycins II and III, the main components of the carminomycin complex were isolated in pure state. Their crystalline exalates and acetate of cardminomycin II were prepared. The PMR spectra of both carminomycins and the 13C-NMR spectra of the oxalates were obtained. The molecular weights of the antibiotics were determined by mass-spectrometry. On the basis of the PMR spectra it was shown that carminomycins II and III had similar structures and differed in the stereoisomerism of the nitrogen-free fragment linked to the amino sugar. This was confirmed by the 13C-NMR spectra. The above fragment (C7H15O3) is analogous to the fragment of baumycins A1 and A2 described earlier.
Asunto(s)
Carubicina/análisis , Daunorrubicina/análogos & derivados , Novobiocina/análogos & derivados , Acetatos/análisis , Fenómenos Químicos , Química , Cromatografía en Gel , Cromatografía en Capa Delgada , Espectroscopía de Resonancia por Spin del Electrón , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Peso Molecular , Novobiocina/análisis , Novobiocina/aislamiento & purificación , Oxalatos/análisisRESUMEN
Three components differing by their properties from the carminomycins described earlier were isolated from the carminomycin complex. Comparison of the IR and UV spectra, as well as chromatographic and physicochemical properties of 2 of them showed that they were dihydrocarminomycin and its aglycone or dihydrocarminomycinone, which was prepared earlier by synthesis. The third component was a chromophore belonging to 1,4,6-trihydroxyanthraquinone. Investigation of its IR spectrum, physicochemica properties and PMR spectrum showed it to be carboxymethylethylcarminomycinone identical to epsilon-rodomycinone. The data were confirmed by 13C-NMR spectrometry.
Asunto(s)
Carubicina/análisis , Daunorrubicina/análogos & derivados , Fenómenos Químicos , Química Física , Cromatografía en Gel , Cristalización , Espectroscopía de Resonancia por Spin del Electrón , Glicósidos/análisis , Espectroscopía de Resonancia Magnética , Espectrofotometría Infrarroja , Espectrofotometría UltravioletaAsunto(s)
Actinomycetales/metabolismo , Antibióticos Antineoplásicos/aislamiento & purificación , Antibióticos Antineoplásicos/análisis , Antibióticos Antineoplásicos/biosíntesis , Fenómenos Químicos , Química Física , Cromatografía en Capa Delgada , Espectrometría de Masas , Naftoquinonas/análisis , Naftoquinonas/biosíntesis , Polienos/análisis , Polienos/biosíntesis , Espectrofotometría InfrarrojaRESUMEN
The structure of nocamycin, a new antitumor antibiotic, has been elucidated with the aid of mass- and PMR-spectroscopic investigation of the antibiotic and its various chemical transformation products. Nocamycin is structurally related to tirandamycins.
Asunto(s)
Antibióticos Antineoplásicos , Fenómenos Químicos , Química , Espectroscopía de Resonancia Magnética , Espectrometría de MasasRESUMEN
A method for qualitative and quantitative investigation of rubomycin composition was developed. It was based on chromatographic separation of the antibiotic components in a thin layer of silicic acid followed by their elution and spectrophotometric determination. An additional component of rubomycin found in the therapeutic preparations and the conditions of its formation were studied. Fifteen different lots of rubomycin were assayed and the requirements for the limits of the admixtures in the antibiotic therapeutic preparations were determined.