RESUMEN
We used viral intersectional tools to map the entire projectome of corticospinal neurons associated with fine distal forelimb control in Fischer 344 rats and rhesus macaques. In rats, we found an extraordinarily diverse set of collateral projections from corticospinal neurons to 23 different brain and spinal regions. Remarkably, the vast weighting of this "motor" projection was to sensory systems in both the brain and spinal cord, confirmed by optogenetic and transsynaptic viral intersectional tools. In contrast, rhesus macaques exhibited far heavier and narrower weighting of corticospinal outputs toward spinal and brainstem motor systems. Thus, corticospinal systems in macaques primarily constitute a final output system for fine motor control, whereas this projection in rats exerts a multi-modal integrative role that accesses far broader CNS regions. Unique structural-functional correlations can be achieved by mapping and quantifying a single neuronal system's total axonal output and its relative weighting across CNS targets.
Asunto(s)
Corteza Motora , Tractos Piramidales , Animales , Axones/fisiología , Mapeo Encefálico , Macaca mulatta , Corteza Motora/fisiología , Tractos Piramidales/fisiología , Ratas , Médula Espinal/fisiologíaRESUMEN
Inhibitory extracellular matrices form around mature neurons as perineuronal nets containing chondroitin sulfate proteoglycans that limit axonal sprouting after CNS injury. The enzyme chondroitinase (Chase) degrades inhibitory chondroitin sulfate proteoglycans and improves axonal sprouting and functional recovery after spinal cord injury in rodents. We evaluated the effects of Chase in rhesus monkeys that had undergone C7 spinal cord hemisection. Four weeks after hemisection, we administered multiple intraparenchymal Chase injections below the lesion, targeting spinal cord circuits that control hand function. Hand function improved significantly in Chase-treated monkeys relative to vehicle-injected controls. Moreover, Chase significantly increased corticospinal axon growth and the number of synapses formed by corticospinal terminals in gray matter caudal to the lesion. No detrimental effects were detected. This approach appears to merit clinical translation in spinal cord injury.
Asunto(s)
Condroitinasas y Condroitín Liasas/uso terapéutico , Traumatismos de la Médula Espinal/tratamiento farmacológico , Animales , Axones/patología , Condroitinasas y Condroitín Liasas/administración & dosificación , Condroitinasas y Condroitín Liasas/efectos adversos , Sustancia Gris/patología , Mano/inervación , Mano/fisiopatología , Inyecciones Intralesiones , Macaca mulatta , Masculino , Microglía/patología , Neuronas Motoras/patología , Desempeño Psicomotor , Tractos Piramidales/patología , Recuperación de la Función , Traumatismos de la Médula Espinal/fisiopatología , Porcinos , Sinapsis/patología , Resultado del TratamientoRESUMEN
We grafted human spinal cord-derived neural progenitor cells (NPCs) into sites of cervical spinal cord injury in rhesus monkeys (Macaca mulatta). Under three-drug immunosuppression, grafts survived at least 9 months postinjury and expressed both neuronal and glial markers. Monkey axons regenerated into grafts and formed synapses. Hundreds of thousands of human axons extended out from grafts through monkey white matter and synapsed in distal gray matter. Grafts gradually matured over 9 months and improved forelimb function beginning several months after grafting. These findings in a 'preclinical trial' support translation of NPC graft therapy to humans with the objective of reconstituting both a neuronal and glial milieu in the site of spinal cord injury.
Asunto(s)
Regeneración Nerviosa , Células-Madre Neurales/trasplante , Médula Espinal/fisiopatología , Animales , Axones/metabolismo , Diferenciación Celular , Movimiento Celular , Supervivencia Celular , Humanos , Macaca mulatta , Imagen por Resonancia Magnética , Masculino , Células-Madre Neurales/citología , Médula Espinal/patología , Médula Espinal/ultraestructura , Resultado del TratamientoRESUMEN
Bone marrow stromal cells (BMSCs) have been reported to exert potential neuroprotective properties in models of neurotrauma, although precise mechanisms underlying their benefits are poorly understood. Despite this lack of knowledge, several clinical trials have been initiated using these cells. To determine whether local mechanisms mediate BMSC neuroprotective actions, we grafted allogeneic BMSCs to sites of severe, compressive spinal cord injury (SCI) in Sprague-Dawley rats. Cells were administered 48 h after the original injury. Additional animals received allogeneic MSCs that were genetically modified to secrete brain-derived neurotrophic factor (BDNF) to further determine whether a locally administered neurotrophic factor provides or extends neuroprotection. When assessed 2 months post-injury in a clinically relevant model of severe SCI, BMSC grafts with or without BDNF secretion failed to improve motor outcomes. Thus, allogeneic grafts of BMSCs do not appear to act through local mechanisms, and future clinical trials that acutely deliver BMSCs to actual sites of injury within days are unlikely to be beneficial. Additional studies should address whether systemic administration of BMSCs alter outcomes from neurotrauma.
Asunto(s)
Trasplante de Médula Ósea/métodos , Factor Neurotrófico Derivado del Encéfalo/metabolismo , Actividad Motora , Traumatismos de la Médula Espinal/fisiopatología , Traumatismos de la Médula Espinal/cirugía , Células del Estroma/trasplante , Animales , Conducta Animal/fisiología , Modelos Animales de Enfermedad , Femenino , Actividad Motora/fisiología , Ratas , Ratas Sprague-DawleyRESUMEN
Recent preclinical advances highlight the therapeutic potential of treatments aimed at boosting regeneration and plasticity of spinal circuitry damaged by spinal cord injury (SCI). With several promising candidates being considered for translation into clinical trials, the SCI community has called for a non-human primate model as a crucial validation step to test efficacy and validity of these therapies prior to human testing. The present paper reviews the previous and ongoing efforts of the California Spinal Cord Consortium (CSCC), a multidisciplinary team of experts from 5 University of California medical and research centers, to develop this crucial translational SCI model. We focus on the growing volumes of high resolution data collected by the CSCC, and our efforts to develop a biomedical informatics framework aimed at leveraging multidimensional data to monitor plasticity and repair targeting recovery of hand and arm function. Although the main focus of many researchers is the restoration of voluntary motor control, we also describe our ongoing efforts to add assessments of sensory function, including pain, vital signs during surgery, and recovery of bladder and bowel function. By pooling our multidimensional data resources and building a unified database infrastructure for this clinically relevant translational model of SCI, we are now in a unique position to test promising therapeutic strategies' efficacy on the entire syndrome of SCI. We review analyses highlighting the intersection between motor, sensory, autonomic and pathological contributions to the overall restoration of function. This article is part of a Special Issue entitled SI: Spinal cord injury.
Asunto(s)
Modelos Animales de Enfermedad , Informática Médica , Plasticidad Neuronal , Recuperación de la Función , Traumatismos de la Médula Espinal/terapia , Regeneración de la Medula Espinal , Animales , Humanos , Macaca mulatta , Actividad Motora , Traumatismos de la Médula Espinal/fisiopatología , Investigación Biomédica Traslacional , Resultado del TratamientoRESUMEN
Efforts to understand spinal cord injury (SCI) and other complex neurotrauma disorders at the pre-clinical level have shown progress in recent years. However, successful translation of basic research into clinical practice has been slow, partly because of the large, heterogeneous data sets involved. In this sense, translational neurological research represents a "big data" problem. In an effort to expedite translation of pre-clinical knowledge into standards of patient care for SCI, we describe the development of a novel database for translational neurotrauma research known as Visualized Syndromic Information and Outcomes for Neurotrauma-SCI (VISION-SCI). We present demographics, descriptive statistics, and translational syndromic outcomes derived from our ongoing efforts to build a multi-center, multi-species pre-clinical database for SCI models. We leveraged archived surgical records, postoperative care logs, behavioral outcome measures, and histopathology from approximately 3000 mice, rats, and monkeys from pre-clinical SCI studies published between 1993 and 2013. The majority of animals in the database have measures collected for health monitoring, such as weight loss/gain, heart rate, blood pressure, postoperative monitoring of bladder function and drug/fluid administration, behavioral outcome measures of locomotion, and tissue sparing postmortem. Attempts to align these variables with currently accepted common data elements highlighted the need for more translational outcomes to be identified as clinical endpoints for therapeutic testing. Last, we use syndromic analysis to identify conserved biological mechanisms of recovery after cervical SCI between rats and monkeys that will allow for more-efficient testing of therapeutics that will need to be translated toward future clinical trials.
Asunto(s)
Bases de Datos Factuales , Traumatismos de la Médula Espinal/fisiopatología , Investigación Biomédica Traslacional , Animales , Biología Computacional , Haplorrinos , Ratones , Modelos Animales , RatasRESUMEN
Primates are an important and unique animal resource. We have developed a nonhuman primate model of spinal cord injury (SCI) to expand our knowledge of normal primate motor function, to assess the impact of disease and injury on sensory and motor function, and to test candidate therapies before they are applied to human patients. The lesion model consists of a lateral spinal cord hemisection at the C7 spinal level with subsequent examination of behavioral, electrophysiological, and anatomical outcomes. Results to date have revealed significant neuroanatomical and functional differences between rodents and primates that impact the development of candidate therapies. Moreover, these findings suggest the importance of testing some therapeutic approaches in nonhuman primates prior to the use of invasive approaches in human clinical trials. Our primate model is intended to: 1) lend greater positive predictive value to human translatable therapies, 2) develop appropriate methods for human translation, 3) lead to basic discoveries that might not be identified in rodent models and are relevant to human translation, and 4) identify new avenues of basic research to "reverse-translate" important questions back to rodent models.
Asunto(s)
Modelos Animales de Enfermedad , Traumatismos de la Médula Espinal/patología , Animales , Vértebras Cervicales , Humanos , Enfermedades del Sistema Nervioso/genética , Enfermedades del Sistema Nervioso/patología , Plasticidad Neuronal/fisiología , Primates , Especificidad de la Especie , Traumatismos de la Médula Espinal/genética , Traumatismos de la Médula Espinal/terapiaRESUMEN
BACKGROUND: Reliable outcome measures are essential for preclinical modeling of spinal cord injury (SCI) in primates. MEASURES: need to be sensitive to both increases and decreases in function in order to demonstrate potential positive or negative effects of therapeutics. OBJECTIVES: To develop behavioral tests and analyses to assess recovery of function after SCI in the nonhuman primate. METHODS: In all, 24 male rhesus macaques were subjected to complete C7 lateral hemisection. The authors scored recovery of function in an open field and during hand tasks in a restraining chair. In addition, EMG analyses were performed in the open field, during hand tasks, and while animals walked on a treadmill. Both control and treated monkeys that received candidate therapeutics were included in this report to determine whether the behavioral assays were capable of detecting changes in function over a wide range of outcomes. RESULTS: The behavioral assays are shown to be sensitive to detecting a wide range of motor functional outcomes after cervical hemisection in the nonhuman primate. Population curves on recovery of function were similar across the different tasks; in general, the population recovers to about 50% of baseline performance on measures of forelimb function. CONCLUSIONS: The behavioral outcome measures that the authors developed in this preclinical nonhuman primate model of SCI can detect a broad range of motor recovery. A set of behavioral assays is an essential component of a model that will be used to test efficacies of translational candidate therapies for SCI.
Asunto(s)
Miembro Anterior/fisiopatología , Lateralidad Funcional/fisiología , Recuperación de la Función/fisiología , Traumatismos de la Médula Espinal/fisiopatología , Animales , Evaluación de la Discapacidad , Modelos Animales de Enfermedad , Estimulación Eléctrica , Electromiografía , Prueba de Esfuerzo , Conducta Exploratoria/fisiología , Locomoción/fisiología , Macaca mulatta , Masculino , Actividad Motora/fisiología , Espasticidad Muscular/diagnóstico , Espasticidad Muscular/etiología , Desempeño Psicomotor/fisiología , Reflejo , Región Sacrococcígea , Factores de TiempoRESUMEN
Neuropathic pain is associated with reorganization of spinal synaptic circuits, implying that adhesion proteins that normally build and modify synapses must be involved. The adhesion proteins E- and N-cadherin delineate different synapses furnished by nociceptive primary afferents, but dynamic aspects of cadherin localization in relationship to onset, maintenance or reversibility of neuropathic pain are uncharacterized. Here, we find very different responses of these cadherins to L5 spinal nerve transection (SNT)-induced mechanical allodynia and to intrathecal glial derived neurotrophic factor (GDNF), which has potent analgesic effects in this pain model. In L5, E-cadherin is rapidly eliminated in patches within lamina IIi contemporaneously with the onset of mechanical allodynia. Intrathecal GDNF in conjunction with, or at 7 days after, L5 SNT prevents or reverses both the loss of E-cadherin and abnormal pain sensation. In contrast, N-cadherin undergoes a delayed and transient increase uniformly across lamina I-II that is insensitive to GDNF. Some N-cadherin-labeled profiles codistribute with GAP-43, suggesting a role in axon sprouting. Patterns of immunolabeling for GDNF receptor components GFRα1, NCAM, and RET after L5 SNT suggest that GFRα1 and NCAM are the principal receptors operative in this model. In addition, GFRα1 codistributes with E-cadherin, but not N-cadherin, profiles. Together, these data indicate strikingly divergent patterns of temporal and molecular regulation of different cadherins at distinct nociceptive circuits in response to spinal nerve injury, suggesting that the two cadherins and the circuits with which they are affiliated participate in different aspects of synaptic and circuit reorganization associated with neuropathic pain.
Asunto(s)
Cadherinas/metabolismo , Regulación de la Expresión Génica/fisiología , Factor Neurotrófico Derivado de la Línea Celular Glial/metabolismo , Neuralgia/patología , Médula Espinal/metabolismo , Médula Espinal/patología , Animales , Modelos Animales de Enfermedad , Proteína GAP-43/metabolismo , Factor Neurotrófico Derivado de la Línea Celular Glial/uso terapéutico , Receptores del Factor Neurotrófico Derivado de la Línea Celular Glial/metabolismo , Hiperalgesia/tratamiento farmacológico , Hiperalgesia/fisiopatología , Masculino , Moléculas de Adhesión de Célula Nerviosa/metabolismo , Neuralgia/tratamiento farmacológico , Neuralgia/etiología , Proteína Quinasa C/metabolismo , Proteínas Proto-Oncogénicas c-ret/metabolismo , Ratas , Ratas Sprague-Dawley , Traumatismos de la Médula Espinal/complicaciones , Factores de TiempoRESUMEN
Although axonal regeneration after CNS injury is limited, partial injury is frequently accompanied by extensive functional recovery. To investigate mechanisms underlying spontaneous recovery after incomplete spinal cord injury, we administered C7 spinal cord hemisections to adult rhesus monkeys and analyzed behavioral, electrophysiological and anatomical adaptations. We found marked spontaneous plasticity of corticospinal projections, with reconstitution of fully 60% of pre-lesion axon density arising from sprouting of spinal cord midline-crossing axons. This extensive anatomical recovery was associated with improvement in coordinated muscle recruitment, hand function and locomotion. These findings identify what may be the most extensive natural recovery of mammalian axonal projections after nervous system injury observed to date, highlighting an important role for primate models in translational disease research.
Asunto(s)
Plasticidad Neuronal/fisiología , Tractos Piramidales/fisiología , Reclutamiento Neurofisiológico/fisiología , Traumatismos de la Médula Espinal/fisiopatología , Animales , Electromiografía/métodos , Femenino , Macaca mulatta , Masculino , Actividad Motora/fisiologíaRESUMEN
Primate models of spinal cord injury differ from rodent models in several respects, including the relative size and functional neuroanatomy of spinal projections. Fundamental differences in scale raise the possibility that retrograde injury signals, and treatments applied at the level of the spinal cord that exhibit efficacy in rodents, may fail to influence neurons at the far greater distances of primate systems. Thus, we examined both local and remote neuronal responses to neurotrophic factor-secreting cell grafts placed within sites of right C7 hemisection lesions in the rhesus macaque. Six months after gene delivery of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) into C7 lesion sites, we found both local effects of growth factors on axonal growth, and remote effects of growth factors reflected in significant reductions in axotomy-induced atrophy of large pyramidal neurons within the primary motor cortex. Additional examination in a rodent model suggested that BDNF, rather than NT-3, mediated remote protection of corticospinal neurons in the brain. Thus, injured neural systems retain the ability to respond to growth signals over the extended distances of the primate CNS, promoting local axonal growth and preventing lesion-induced neuronal degeneration at a distance. Remote cortical effects of spinally administered growth factors could "prime" the neuron to respond to experimental therapies that promote axonal plasticity or regeneration.
Asunto(s)
Factor Neurotrófico Derivado del Encéfalo/metabolismo , Regeneración Nerviosa/fisiología , Neurotrofina 3/metabolismo , Traumatismos de la Médula Espinal/metabolismo , Animales , Atrofia/patología , Atrofia/prevención & control , Axones/fisiología , Factor Neurotrófico Derivado del Encéfalo/administración & dosificación , Corteza Cerebral/metabolismo , Corteza Cerebral/patología , Vértebras Cervicales/lesiones , Modelos Animales de Enfermedad , Femenino , Inyecciones Espinales , Macaca mulatta , Neuronas Motoras/metabolismo , Neuronas Motoras/patología , Neurotrofina 3/administración & dosificación , Ratas , Ratas Endogámicas F344 , Receptor trkB/metabolismo , Receptor trkC/metabolismoRESUMEN
To examine neuroanatomical mechanisms underlying fine motor control of the primate hand, adult rhesus monkeys underwent injections of biotinylated dextran amine (BDA) into the right motor cortex. Spinal axonal anatomy was examined using detailed serial-section reconstruction and modified stereological quantification. Eighty-seven percent of corticospinal tract (CST) axons decussated in the medullary pyramids and descended through the contralateral dorsolateral tract of the spinal cord. Eleven percent of CST axons projected through the dorsolateral CST ipsilateral to the hemisphere of origin, and 2% of axons projected through the ipsilateral ventromedial CST. Notably, corticospinal axons decussated extensively across the spinal cord midline. Remarkably, nearly 2-fold more CST axons decussated across the cervical spinal cord midline (approximately 12,000 axons) than were labeled in all descending components of the CST (approximately 6,700 axons). These findings suggest that CST axons extend multiple segmental collaterals. Furthermore, serial-section reconstructions revealed that individual axons descending in either the ipsilateral or contralateral dorsolateral CST can: 1) terminate in the gray matter ipsilateral to the hemisphere of origin; 2) terminate in the gray matter contralateral to the hemisphere of origin; or 3) branch in the spinal cord and terminate on both sides of the spinal cord. These results reveal a previously unappreciated degree of bilaterality and complexity of corticospinal projections in the primate spinal cord. This bilaterality is more extensive than that of the rat CST, and may resemble human CST organization. Thus, augmentation of sprouting of these extensive bilateral CST projections may provide a novel target for enhancing recovery after spinal cord injury.
Asunto(s)
Macaca mulatta/anatomía & histología , Corteza Motora/anatomía & histología , Terminaciones Nerviosas , Fibras Nerviosas , Vías Nerviosas/anatomía & histología , Tractos Piramidales/anatomía & histología , Médula Espinal/anatomía & histología , Animales , Axones , Biotina/administración & dosificación , Biotina/análogos & derivados , Biotina/metabolismo , Dextranos/administración & dosificación , Dextranos/metabolismo , Femenino , Colorantes Fluorescentes/administración & dosificación , Colorantes Fluorescentes/metabolismo , Masculino , Microinyecciones , Corteza Motora/citología , Vías Nerviosas/citología , Tractos Piramidales/citología , Médula Espinal/citologíaRESUMEN
Cadherins are synaptically enriched cell adhesion and signaling molecules. In brain, they function in axon targeting and synaptic plasticity. In adult spinal cord, their localization, synaptic affiliation, and role in injury-related plasticity are mostly unexplored. Here, we demonstrate in adult rat dorsal horn that E- and N-cadherin display unique patterns of localization to functionally distinct types of synapses of intrinsic and primary afferent origin. Within the nociceptive afferent pathway to lamina II, nonpeptidergic C-fiber synapses in the deeper half of lamina II (IIi) contain E-cadherin but mostly lack N-cadherin, whereas the majority of the peptidergic C-fiber synapses in the outer half of lamina II (IIo) contain N-cadherin but lack E-cadherin. Approximately one-half of the Abeta-fiber terminations in lamina III contain N-cadherin; none contain E-cadherin. Strikingly, the distribution and levels of these cadherins are differentially affected by sciatic nerve axotomy, a model of neuropathic pain in which degenerative and regenerative structural plasticity has been implicated. Within the first 7 d after axotomy, E-cadherin is rapidly and completely lost from the dorsal horn synapses with which it is affiliated, whereas N-cadherin localization and levels are unchanged; such patterns persist through 28 d postlesion. The loss of E-cadherin thus occurs before the onset of mechanical hyperalgesia (approximately 10-21 d postlesion), as reported previously. Together, the synaptic specificity displayed by these cadherins, coupled with their differential response to injury, suggests that they may proactively contribute to the maintenance of some, and incipient dismantling of other, synaptic circuits in response to nerve injury. Speculatively, such changes may ultimately contribute to subsequently emerging abnormalities in pain perception.
Asunto(s)
Cadherinas/análisis , Células del Asta Posterior/química , Nervio Ciático/lesiones , Sinapsis/química , Animales , Axotomía , Cadherinas/metabolismo , Masculino , Plasticidad Neuronal , Células del Asta Posterior/metabolismo , Ratas , Ratas Sprague-Dawley , Médula Espinal/citologíaRESUMEN
Vegetative regeneration of individual genotypes of Asian Reynoutria taxa, which are invasive in the Czech Republic, was studied in R. sachalinensis (five genotypes), R. japonica (a single genotype present in the country), and their hybrid R. ×bohemica (nine genotypes). Identity of genotypes was confirmed by isozyme analysis. Ten rhizome segments of each genotype were planted in a randomized block design. After 30 d, the regeneration rate of each genotype was measured as the proportion of rhizomes that produced shoots. Emergence time and final mass of each shoot were recorded. The regeneration rate and final shoot mass were significantly affected by genotype in R. ×bohemica but not in R. sachalinensis. In R. ×bohemica, easily regenerating genotypes grew faster. Regeneration characteristics that crucially contribute to the fitness of these vegetatively spreading plants are closely related to each other. In genotypes with a low regeneration rate, early-emerging shoots produced more biomass, while in those with a high regeneration rate, shoot mass was independent of emergence time. Mean clone size recorded in the field was marginally significantly related to emergence time during regeneration; regeneration characteristics might thus affect the extent of R. ×bohemica invasion at a regional scale. Hybrids genetically intermediate between the parents regenerated better than those closely related to parents. Novel hybrid invasive genotypes may be produced by rare sexual reproduction, fixed by clonal growth, and present a previously unknown threat to native vegetation.