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1.
Food Microbiol ; 109: 104150, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-36309452

RESUMEN

Routine monitoring of foodborne pathogens such as Listeria monocytogenes in food processing environments are time-consuming necessities to ensure food safety. Alternative rapid diagnostic methods for pathogen detection are increasingly used, but often demand specialized equipment, making them unsuitable for on-site testing. This short communication describes the successful demonstration of combining the sample preparation method Matrix-Lysis with a chemiluminescent based detection platform (AquaSpark™) for detection of L. monocytogenes in milk and yogurt. The proposed method was evaluated against qPCR resulting in 100% relative specificity for both foodstuffs and a relative sensitivity of 100% for milk as well as 96% for yogurt for bacterial levels >1 CFU/ml. Only at very low initial bacterial concentrations (<1 CFU/ml) diverging results were found highlighting the advantages and limitations of both methods. While being less susceptible to contamination and false positive results from non-growing or dead cells, qPCR had a slightly lower overall detection limit. However, it has to be pointed out that qPCR has an increased analytical cost and also requires an additional 24 h analysis time. This study demonstrates the first successful application of a chemilumonogenic detection approach for L. monocytogenes in food that has a high potential for on-site testing.


Asunto(s)
Listeria monocytogenes , Animales , Listeria monocytogenes/genética , Microbiología de Alimentos , Productos Lácteos/microbiología , Leche/microbiología , Reacción en Cadena en Tiempo Real de la Polimerasa , Yogur
2.
Sci Rep ; 11(1): 17545, 2021 09 02.
Artículo en Inglés | MEDLINE | ID: mdl-34475462

RESUMEN

The importance of virus disease outbreaks and its prevention is of growing public concern but our understanding of virus transmission routes is limited by adequate sampling strategies. While conventional swabbing methods provide merely a microbial snapshot, an ideal sampling strategy would allow reliable collection of viral genomic data over longer time periods. This study has evaluated a new, paper-based sticker approach for collection of reliable viral genomic data over longer time periods up to 14 days and after implementation of different hygiene measures. In contrast to swabbing methods, which sample viral load present on a surface at a given time, the paper-based stickers are attached to the surface area of interest and collect viruses that would have otherwise been transferred onto that surface. The major advantage of one-side adhesive stickers is that they are permanently attachable to a variety of surfaces. Initial results demonstrate that stickers permit stable recovery characteristics, even at low virus titers. Stickers also allow reliable virus detection after implementation of routine hygiene measures and over longer periods up to 14 days. Overall, results for this new sticker approach for virus genomic data collection are encouraging, but further studies are required to confirm anticipated benefits over a range of virus types.


Asunto(s)
Virosis/virología , Virus/aislamiento & purificación , ADN Viral/análisis , ADN Viral/genética , Desinfección , Humanos , Reacción en Cadena de la Polimerasa , Propiedades de Superficie , Virosis/epidemiología , Virosis/prevención & control , Virus/genética
3.
Int J Mol Sci ; 19(3)2018 Mar 09.
Artículo en Inglés | MEDLINE | ID: mdl-29522483

RESUMEN

For three decades now, ionic liquids (ILs), organic salts comprising only ions, have emerged as a new class of pharmaceuticals. Although recognition of the antimicrobial effects of ILs is growing rapidly, there is almost nothing known about their possible virucidal activities. This probably reflects the paucity of understanding virus inactivation. In this study, we performed a systematic analysis to determine the effect of specific structural motifs of ILs on three different biological test systems (viruses, bacteria and enzymes). Overall, the effects of 27 different ILs on two non-enveloped and one enveloped virus (P100, MS2 and Phi6), two Gram negative and one Gram positive bacteria (E. coli, P. syringae and L. monocytogenes) and one enzyme (Taq DNA polymerase) were investigated. Results show that while some ILs were virucidal, no clear structure activity relationships (SARs) could be identified for the non-enveloped viruses P100 and MS2. However, for the first time, a correlation has been demonstrated between the effects of ILs on enveloped viruses, bacteria and enzyme inhibition. These identified SARs serve as a sound starting point for further studies.


Asunto(s)
Antivirales/farmacología , Virus ADN/efectos de los fármacos , Líquidos Iónicos/farmacología , Virus ARN/efectos de los fármacos , Antivirales/química , Escherichia coli/efectos de los fármacos , Humanos , Líquidos Iónicos/química , Listeria monocytogenes/efectos de los fármacos , Pseudomonas syringae/efectos de los fármacos , Relación Estructura-Actividad , Polimerasa Taq/efectos de los fármacos
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