Combination of phytase and citric acid, but not phytase alone, ensures regular rates of growth and bone mineralization in quails under severe phosphorus restriction.

An experiment was conducted to evaluate the effects of phytase (PHY), alone or in combination with citric acid (CA), on growing Japanese quails under moderate and severe phosphorus (P) restriction. For this, 504 one-day-old female quails were distributed in a completely randomised design with seven treatments and six replications. Treatments consisted of the following diets: (a) positive control (PC): formulated according to quails nutritional requirements; (b) negative control 40% (NC40 ): PC with 40% less aP; (c) NC40 +PHY; (d) NC40 +PHY+CA; (e) negative control 65% (NC65 ): PC with 65% less aP; (f) NC65 +PHY; (g) NC65 +PHY+CA. Phytase and CA were included at 300 FTU/kg and 40 g/kg, respectively. Performance, bone mineralization and plasma mineral concentration were evaluated at day 35; calcium (Ca) and P metabolism, between days 36 and 39. No significant differences in performance, plasma Ca and P were detected between quails fed PC or NC40 ; however, NC40 decreased tibia ash (p < 0.01). Treatment NC65 impaired daily weight gain (p = 0.01), tibia ash (p < 0.01), and tibia optical densitometry (p = 0.03), as well as modified plasma Ca (p < 0.01) and P (p = 0.03) concentrations when compared to PC. Both NC40 and NC65 reduced P excretion (p < 0.01). Phytase alone restored tibia ash of NC40 quails to regular rates. Nevertheless, only PHY and CA combined returned plasma P, tibia ash, tibia optical densitometry, and daily weight gain of quails fed NC65 to similar values as those observed in PC. In conclusion, PHY alone suppresses the negative effects of moderate P restriction in growing Japanese quails, but fails in this task under severe P restriction. In such a situation, only the combination of PHY and CA ensures regular rates of growth and bone mineralization. This concept should be better explored by producers in order to take full advantage of the reduction in P excretion achieved by lowering dietary P concentrations.

1, 25-dihydroxycholecalciferol from Solanum glaucophyllum supports normal growth and reduces the negative effects of calcium and phosphorus restriction on broilers' bone tissue / 1, 25 dihidroxicolecalciferol de origem herbal (Solanum glaucophyllum) suporta o desempenho normal e diminui os efeitos negativos da restrição de cálcio e fósforo sobre o desenvolvimento ósseo de frangos de corte

Feed supplementation with 1,25-dihydroxycholecalciferol (1,25(OH)2D3) has been studied as a means to decrease the incidence of bone abnormalities in broilers and reduce dietary inclusion of calcium and phosphorus. The objective of this research was to determine the optimal level of feed supplementation with 1,25(OH)2D3 from Solanum glaucophyllum for growing broilers restricted in calcium and available phosphorus. A total of 648 day-old male chicks were distributed in a completely randomized design with six dietary treatments and six replications of 18 birds. Treatments consisted of one positive control (PC: corn-soybean-meal diet formulated to reach or exceed Rostagno et al. (2011) nutritional recommendations), one negative control (NC: PC diet with 15% reduction of calcium and available phosphorus), and four NC diets supplemented with 0.5, 1.0, 1.5, or 2.0 µg kg-¹ of 1,25(OH)2D3. Performance and bone development characteristics were evaluated at days 21 and 33. Negative control did not modify broilers' performance in comparison with PC, but birds fed with NC plus 1.0 µg kg-¹ of 1,25(OH)2D3 showed higher (p=0.01) daily weight gain than PC at day 21. Negative control reduced (p < 0.01) tibia weight, ash, breaking strength, and Seedor index at day 21, but supplementation with 1,25(OH)2D3 mitigated all these negative effects. A quadratic effect of 1,25(OH)2D3 level was found for daily weight gain (p=0.03), tibia weight (p < 0.01), breaking strength (p < 0.01), and Seedor index (p < 0.01) at this age. At day 33, NC broilers still had lower tibia weight (p=0.01), ash (p < 0.01), and Seedor index (p < 0.01) than those fed PC. Only tibia ash did not return to the same value as that observed in PC after NC supplementation with 1,25(OH)2D3. A quadratic effect (p < 0.01) of 1,25(OH)2D3 level was found for tibia ash and Seedor index at this age. On average, dietary supplementation of 1.15 µg kg-¹ of 1,25(OH)2D3 was identified as the optimal level for bone...(AU)

A suplementação da dieta com 1,25-dihidroxicolecalciferol (1,25(OH)2D3) vem sendo estudada como alternativa para redução da incidência de desordens esqueléticas em frangos de corte e diminuição nos níveis dietéticos de cálcio e fósforo. O objetivo deste trabalho foi determinar o melhor nível de inclusão de 1,25(OH)2D3 de origem herbal (Solanum glaucophyllum) para frangos restritos em cálcio e fósforo. Para tanto, 648 pintainhos de corte machos de um dia foram distribuídos em um delineamento inteiramente casualizado com seis tratamentos e seis repetições de 18 aves. Os tratamentos consistiram em um controle positivo (CP: dieta à base de milho e farelo de soja, formulada para atender às recomendações nutricionais de Rostagno et al. (2011), um controle negativo (CN: dieta CP com 15% de redução nos níveis de cálcio e fósforo disponível), e quatro dietas CN suplementadas com 0,5; 1,0; 1,5 ou 2,0 µg kg-¹ de 1,25(OH)2D3. Desempenho produtivo e características ósseas foram avaliados nos dias 21 e 33. O consumo do CN não reduziu o desempenho das aves em comparação ao CP; porém, as aves alimentadas com o CN+1,0 µg kg-¹ de 1,25(OH)2D3 apresentaram maior (p=0,01) ganho de peso do que as alimentadas com o CP aos 21 dias. A dieta CN reduziu (p < 0,01) o peso, a matéria mineral, a força de quebra e o índice de Seedor da tíbia aos 21 dias; porém, a suplementação com 1,25(OH)2D3 reverteu todos esses efeitos adversos. Efeito quadrático dos níveis de 1,25(OH)2D3 foi observado para ganho de peso (p=0,03), peso da tíbia (p < 0.01), força de quebra (p < 0,01), e índice de Seedor (p < 0,01) nessa idade. Aos 33 dias, frangos alimentados com a dieta CN continuaram apresentando menores peso de tíbia (p=0,01), matéria mineral (p < 0,01) e índice de Seedor (p < 0,01) do que os que receberam a dieta CP. Somente a matéria mineral da tíbia não retornou a valor semelhante ao observado nas aves CP após a suplementação com 1,25(OH)2D3. Efeito quadrático (p < 0,01) dos níveis de...(AU)

1, 25-dihydroxycholecalciferol from Solanum glaucophyllum supports normal growth and reduces the negative effects of calcium and phosphorus restriction on broilers' bone tissue / 1, 25 dihidroxicolecalciferol de origem herbal (Solanum glaucophyllum) suporta o desempenho normal e diminui os efeitos negativos da restrição de cálcio e fósforo sobre o desenvolvimento ósseo de frangos de corte

Feed supplementation with 1,25-dihydroxycholecalciferol (1,25(OH)2D3) has been studied as a means to decrease the incidence of bone abnormalities in broilers and reduce dietary inclusion of calcium and phosphorus. The objective of this research was to determine the optimal level of feed supplementation with 1,25(OH)2D3 from Solanum glaucophyllum for growing broilers restricted in calcium and available phosphorus. A total of 648 day-old male chicks were distributed in a completely randomized design with six dietary treatments and six replications of 18 birds. Treatments consisted of one positive control (PC: corn-soybean-meal diet formulated to reach or exceed Rostagno et al. (2011) nutritional recommendations), one negative control (NC: PC diet with 15% reduction of calcium and available phosphorus), and four NC diets supplemented with 0.5, 1.0, 1.5, or 2.0 µg kg-¹ of 1,25(OH)2D3. Performance and bone development characteristics were evaluated at days 21 and 33. Negative control did not modify broilers' performance in comparison with PC, but birds fed with NC plus 1.0 µg kg-¹ of 1,25(OH)2D3 showed higher (p=0.01) daily weight gain than PC at day 21. Negative control reduced (p < 0.01) tibia weight, ash, breaking strength, and Seedor index at day 21, but supplementation with 1,25(OH)2D3 mitigated all these negative effects. A quadratic effect of 1,25(OH)2D3 level was found for daily weight gain (p=0.03), tibia weight (p < 0.01), breaking strength (p < 0.01), and Seedor index (p < 0.01) at this age. At day 33, NC broilers still had lower tibia weight (p=0.01), ash (p < 0.01), and Seedor index (p < 0.01) than those fed PC. Only tibia ash did not return to the same value as that observed in PC after NC supplementation with 1,25(OH)2D3. A quadratic effect (p < 0.01) of 1,25(OH)2D3 level was found for tibia ash and Seedor index at this age. On average, dietary supplementation of 1.15 µg kg-¹ of 1,25(OH)2D3 was identified as the optimal level for bone...

A suplementação da dieta com 1,25-dihidroxicolecalciferol (1,25(OH)2D3) vem sendo estudada como alternativa para redução da incidência de desordens esqueléticas em frangos de corte e diminuição nos níveis dietéticos de cálcio e fósforo. O objetivo deste trabalho foi determinar o melhor nível de inclusão de 1,25(OH)2D3 de origem herbal (Solanum glaucophyllum) para frangos restritos em cálcio e fósforo. Para tanto, 648 pintainhos de corte machos de um dia foram distribuídos em um delineamento inteiramente casualizado com seis tratamentos e seis repetições de 18 aves. Os tratamentos consistiram em um controle positivo (CP: dieta à base de milho e farelo de soja, formulada para atender às recomendações nutricionais de Rostagno et al. (2011), um controle negativo (CN: dieta CP com 15% de redução nos níveis de cálcio e fósforo disponível), e quatro dietas CN suplementadas com 0,5; 1,0; 1,5 ou 2,0 µg kg-¹ de 1,25(OH)2D3. Desempenho produtivo e características ósseas foram avaliados nos dias 21 e 33. O consumo do CN não reduziu o desempenho das aves em comparação ao CP; porém, as aves alimentadas com o CN+1,0 µg kg-¹ de 1,25(OH)2D3 apresentaram maior (p=0,01) ganho de peso do que as alimentadas com o CP aos 21 dias. A dieta CN reduziu (p < 0,01) o peso, a matéria mineral, a força de quebra e o índice de Seedor da tíbia aos 21 dias; porém, a suplementação com 1,25(OH)2D3 reverteu todos esses efeitos adversos. Efeito quadrático dos níveis de 1,25(OH)2D3 foi observado para ganho de peso (p=0,03), peso da tíbia (p < 0.01), força de quebra (p < 0,01), e índice de Seedor (p < 0,01) nessa idade. Aos 33 dias, frangos alimentados com a dieta CN continuaram apresentando menores peso de tíbia (p=0,01), matéria mineral (p < 0,01) e índice de Seedor (p < 0,01) do que os que receberam a dieta CP. Somente a matéria mineral da tíbia não retornou a valor semelhante ao observado nas aves CP após a suplementação com 1,25(OH)2D3. Efeito quadrático (p < 0,01) dos níveis de...