RESUMEN
Sclerotinia sclerotiorum (Sclerotiniaceae) is a plant pathogenic fungus that causes white mold disease in vegetable crops, including the common bean (Phaseolus vulgaris). Proteases produced by fungi are normally an important part of the pathogenic process in the host. We examined the effect of different carbon sources--pectin, glucose, and cell wall of P. vulgaris on the production of proteases in cultures of S. sclerotiorum. These proteases were also assayed in infected P. vulgaris plants. Enzyme activity was increased with all carbon sources, but the highest levels were found when pectin was added. Based on real-time quantitative reverse transcription-PCR analyses, protease induction in S. sclerotiorum was found to occur at the level of gene transcription. The finding of increased expression of acid phosphatase 1 and aspartyl protease in vivo in infected P. vulgaris plants supports the role of these enzymes in the invasion process of S. sclerotiorum.
Asunto(s)
Ascomicetos/enzimología , Ascomicetos/fisiología , Carbono/farmacología , Péptido Hidrolasas/metabolismo , Phaseolus/microbiología , Enfermedades de las Plantas/microbiología , Ascomicetos/efectos de los fármacos , Ascomicetos/genética , Medios de Cultivo/química , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Regulación Fúngica de la Expresión Génica/efectos de los fármacos , Genes Fúngicos/genética , Concentración de Iones de Hidrógeno/efectos de los fármacos , Cinética , Péptido Hidrolasas/genética , Phaseolus/efectos de los fármacos , Inhibidores de Proteasas/farmacología , Proteolisis/efectos de los fármacos , Reacción en Cadena en Tiempo Real de la Polimerasa , Transcripción Genética/efectos de los fármacosRESUMEN
Complementary or alternative modalities of medical treatment have been gaining attention as primary or supplementary therapies in cardiovascular disease pain management. However, definitive research in these areas has been limited by the inability to perform placebo-controlled trials when evaluating these treatments. Preliminary studies have suggested a possible benefit from acupuncture, electrical nerve stimulation, and spinal cord stimulation in the treatment of patients with angina pectoris and coronary artery disease.
