RESUMEN
La Psoriais se caracteriza por incremento del ciclo celular a nivel epidérmico, evidenciado a nivel histopatológico por un intensa hiperparaqueratosis, acantosis, papilomatosis e inflamación crónica. Materiales y métodos: Se estudió con microscopia óptica de alta resolución (MOAR), la epidermis de pacientes portadores de Psoriasis, analizando, la diferenciación epitelial y las posibelas alteraciones estructurales de los queratinocitos. Resultados: Los especímenes correspondieron a 10 pacientes con diagnóstico clínico e histopatológico de psoriasis. Las biopsias de piel fueron fijadas en solución de glutaraldehido buffer-collidina durante 48hs, procesados con la técnica para MOAR y coloreados con azul de toluidina, azul de metileno, fuscina básica y metenamina-plata. Los elementos epiteliales basales presentaban núcleos ovoideos, varios de ellos con nucleólos prominentes. En 7 de los casos estudiados el estrato granuloso estuvo ausente y en los 3 casos restantes observamos retención de núcleos y nucleólos. Conclusiones: Seobservaron queratinocitos con anfofilia e infiltrado linfocitario y de macrofagos.
Skin tissue from patients with Psoriasis was analyzed using HROM (High Resolution Optical Microscopy), studying epithelial differentiation and possible structural alterations of the queratinocytes. The samples were taken from 10 patients with histopathologic diagnosis of Psoriasis. This tissue samples where affixed with glutaraldehide buffer-collidine for 48 hours. Later processed with the HROM technique and colored with toluidine blue, metilene blue, basic Fuscine, and silver metenamine. The basal epithelial elements presented ovoid nucleus and most of them had prominent nucleolus. In 7 of the studied cases, the granulose stratus was absent, and thinner in the rest, with nucleus and nucleolus retention. At this level queratinocytes where observed with perinuclear anfofilia, as well as linfocitic and macrophagic infiltrate and union complex where elongated.
Asunto(s)
Humanos , Células Epiteliales/ultraestructura , Microscopía/métodos , Psoriasis/patología , Piel/patología , Antígenos CD/análisis , Colorantes , Matriz Extracelular , Inflamación/patología , Óptica y Fotónica , Linfocitos T/ultraestructuraRESUMEN
Vista la necesidad de capacitar el Recurso Humano para el funcionamiento de un sistema de salud, en 1999 se reformulo el sistema sanitario e la provincia de Córdoba con una propuesta de reorganizacion y reorientacion. Se ha trabajado en forma conjunta con la Universida Nacional de Córdoba, Consejo Medico, Circulo Medico, Sociedades Cientificas y Deontologicas para acordar una nueva filosofia de trabajo en salud y el numero de recursos humanos a formar. El presente trabajo muestra la estimacion del numero de profesionales medicos necesarios para la provincia, analiza las necesidades de formacion de las distintas especialidades en funcion del nuevo modelo sanitario, como si tambien la distribucion de los mismos. Asi mismo concluye diciendo que el Ministro de Salud y la UNC han logrado en los ultimos cuatro a±os coordinar las politicas de salud, para ofrecer capacitacion acorde a las politicas establecidas. ABSTRACT: Due to the need of human resources training for the implementation of a the new health system in 1999 the sanitary system of the province of Córdoba was reformulated with a new reorganization and reoreintation proposal. Tha National Unversity of Córdoba, The Board of Physicians, the Medicla Society and the Deontologic and Scientific Association have woeked together to come to an agreement on the new work philosophy of health and on the number of physicians needed for the province, analizes the need for trainingin the different specialies based on the new sanitary model as well as their distribution. Likewise, it concludes be saying that, in the lasst four years, the departement and the UNC have been able coordinatea health policy in order to offer trainig in accordance with he established polices
Asunto(s)
Médicos , Internado y Residencia , Recursos HumanosRESUMEN
Vista la necesidad de capacitar el Recurso Humano para el funcionamiento de un sistema de salud, en 1999 se reformulo el sistema sanitario e la provincia de Córdoba con una propuesta de reorganizacion y reorientacion. Se ha trabajado en forma conjunta con la Universida Nacional de Córdoba, Consejo Medico, Circulo Medico, Sociedades Cientificas y Deontologicas para acordar una nueva filosofia de trabajo en salud y el numero de recursos humanos a formar. El presente trabajo muestra la estimacion del numero de profesionales medicos necesarios para la provincia, analiza las necesidades de formacion de las distintas especialidades en funcion del nuevo modelo sanitario, como si tambien la distribucion de los mismos. Asi mismo concluye diciendo que el Ministro de Salud y la UNC han logrado en los ultimos cuatro a±os coordinar las politicas de salud, para ofrecer capacitacion acorde a las politicas establecidas. ABSTRACT: Due to the need of human resources training for the implementation of a the new health system in 1999 the sanitary system of the province of Córdoba was reformulated with a new reorganization and reoreintation proposal. Tha National Unversity of Córdoba, The Board of Physicians, the Medicla Society and the Deontologic and Scientific Association have woeked together to come to an agreement on the new work philosophy of health and on the number of physicians needed for the province, analizes the need for trainingin the different specialies based on the new sanitary model as well as their distribution. Likewise, it concludes be saying that, in the lasst four years, the departement and the UNC have been able coordinatea health policy in order to offer trainig in accordance with he established polices
Asunto(s)
Recursos Humanos , Internado y Residencia , MédicosRESUMEN
BACKGROUND: Allergic contact dermatitis (ACD) is a common human dermatosis in which not all the mechanisms involved in its pathogenesis have been elucidated. OBJECTIVE: To study the expression of CS-1 fibronectin, TARC and Th1-associated chemokine receptors in biopsies from allergic patch test reactions. MATERIAL AND METHODS: Thirteen patients already diagnosed with ACD were challenged on the back with the antigen responsible of the disease and macroscopic responses and biopsies taken after 48 h. Skin biopsies from negative control challenge sites, AD and ICD were also taken. Samples were fixed, embedded in paraffin wax and processed in order to perform histological and immunohistochemical studies. RESULTS: All subjects with ACD showed a positive clinical response and a perivascular mononuclear cell infiltration at 48 h, which was not seen in the negative controls. The majority of skin-infiltrating cells were CD4+ and CD8+ and up to 54% or 40% of them expressed CXCR3 or CCR5, respectively. We also showed expression of CS-1 fibronectin in inflamed endothelial cells not only in ACD but also in AC and ICD. In contrast TARC was only expressed in ACD and AC. CONCLUSION: We showed for the first time that CS-1 fibronectin is expressed in dermal vessels from allergic patch tests positive reactions, as well as irritant and atopic skin lesions.