RESUMEN
ABSTRACT: The entrance of Artificial Intelligence (AI) as a new actor in the doctor-patient relationship has encouraged important legal and ethi-cal considerations among the experts. On the one hand, there is the request to establish a new and dedicated legal background involving AI and AI-related technologies, while others believe there is no need to add new laws in the attempt to define AI's role in healthcare. The aim of this paper is to analyse the possible role of AI in civil liability in healthcare practice, underlining its limits of autonomy in a field where the attribution of liability cannot be uncertain.
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Inteligencia Artificial , Responsabilidad Legal , Humanos , Relaciones Médico-PacienteRESUMEN
BACKGROUND: Teaching ethics in university courses may benefit from different didactic approaches; nonetheless, it still seems unclear whether ethics teaching can be best offered in stand-alone courses or integrated into other courses, or perhaps both. OBJECTIVE: We describe the experience derived from a structured teaching activity in the field of medical ethics, conducted during a lesson for the students of a rehabilitation university second-cycle degree course. METHODS: The participating students were healthcare professionals with different graduate training in rehabilitation. The aim of the lesson was to discuss the essentials of the relationship between patients and rehabilitation healthcare providers, from an inter-professional viewpoint, focused on the principles of trust, mutual respect, power and personal closeness, which are essential components of the therapeutic relationship between patients and physical therapists. RESULT: Shared moral norms guiding the professional conduct of healthcare professionals are a fundamental characteristic of these professions, promoting the public trust in these professions, tearing down barriers to inter-professional collaboration and communication. CONCLUSION: The results are remarkable, and there has been very positive feedback from the students concerning the production of the oath and its contents, as well as about the proposed teaching method, resulting in great interest in clinical ethics.
Asunto(s)
Ética Médica/educación , Profesionalismo/educación , Rehabilitación/educación , Estudiantes , Adulto , Investigación Empírica , Ética Clínica , Femenino , Personal de Salud , Humanos , Masculino , Principios Morales , Aprendizaje Basado en Problemas , Rehabilitación/ética , UniversidadesRESUMEN
Munchausen syndrome by proxy is a relatively rare behavioral disorder affecting a child's primary caregiver, typically the mother. Ethical dilemmas that physicians may face in such situations mainly concern the medical options for best protecting the child's welfare, that are important, in clinical pediatric practice, because critical conflicts might arise between health professionals and parents. In such cases, the physician's primary obligation is to protect the children involved, whose family environment may be essential to their wellbeing. Ev- ery ethical choice should be tailored to a given child's story, which should be viewed as a whole, considering the possible consequences for the family balance, and taking the complexity of the emotional and psychological dimensions of children's relationship with their parents into account. Specific protocols, discussion of clinical cases, open communi- cation of feelings will help doctors to deal more effectively with the families involved and ensure that treatment decisions are made in the child's best interest.
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Trastornos Fingidos/terapia , Síndrome de Munchausen Causado por Tercero/terapia , Médicos/ética , Cuidadores/psicología , Niño , Humanos , Principios Morales , Padres/psicologíaRESUMEN
In the last years an alternative to traditional research projects conducted with patients has emerged: it is represented by the pairing of different type of disease biobanks specimens with Electronic Health Records (EHRs). Even if informed consent remains one of the most contested issues of biobank policy, other ethical challenges still require careful attention, given that additional issues are related to the use of EHRs. In this new way of doing research harmonization of governance is essential in practice, with the aim to make the most use of resources at our disposal, and sharing of samples and data among researchers under common policies regulating the distribution and the use. A biobank-specific Ethics Committee could be seen as a new and type of Ethics Committee, that we suggest to be applied to each biobank, with possible different functions. In particular, considering the possible use of electronic health record data linked to biological specimens in biobanking research, this specific Ethics Committee could draft best practice and ethical guidelines for the utilisation of the EHRs as a tool for genetic research, addressing concerns on accessibility, return of results and privacy and help to educate patients and healthcare providers.
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Bancos de Muestras Biológicas/ética , Registros Electrónicos de Salud/ética , Personal de Salud/ética , Humanos , Consentimiento Informado/éticaRESUMEN
Gender differences, in both clinical and research environment, exist also in a particular category of patients, adolescents, who constitute a vulnerable group with respect to healthcare decisions. In clinical context, the main ethical issues that may be identified within gender medicine for adolescent patients are related to the information given to the patient and its parents, the adolescent's capacity of understanding considering his/her maturity, vulnerability and autonomy, the consent to medical treatment in relation to the different possible approaches to their different efficacy and possible side effects. Also, with regard to the research context, ethical issues may arise from the participation of female minors in clinical trials. Ethical concerns may also arise in the field of resource allocation in health policies, such as the equitable distribution and access to resources, considering the young age of the subjects involved. A bioethical reflection, which takes into account not only the differences biologically and epidemiologically relevant, but also the main determinants of health in adolescence, might find a role in structured education for diversity and gender equity. Given the magnitude of the problem, to encourage the pursuit of gender equity in health and, in some situations, also to promote the full recognition of the right to health of women are some of the most effective and direct ways to reduce inequalities and to ensure a rational and efficient use of available resources, including through a bioethical reflection on the topic. The Authors show the necessity to differentiate the various aspects of gender differences in adolescence medicine, providing arguments in support of the fact that interventions for health prevention and promotion should be modulated in relation to the gender of the recipients, emphasizing the most important aspects for each group of individuals. This approach could implement personalized medicine, even and especially considering gender differences, benefiting from the contribution that a bioethical reflection can provide.
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Salud del Adolescente/ética , Medicina del Adolescente/ética , Equidad en Salud/ética , Disparidades en Atención de Salud/ética , Consentimiento Informado de Menores/ética , Educación del Paciente como Asunto/ética , Sexismo/ética , Adolescente , Femenino , Humanos , Masculino , Factores SexualesRESUMEN
Today, the molecular technique routinely for sex determination in forensics is based the detection of length variations in the X-Y homologous amelogenin gene (AMELX and AMELY). In humans, the amelogenin gene is a single-copy gene located on Xp22.1-Xp22.3 and Yp11.2; the simultaneous detection of the X and Y alleles using polymerase chain reaction (PCR) can lead to gender determination. Several studies have shown that normal males may be typed as females with this test: AMELY deletions may result in no product of amplification and normal males being typed as female as a result of the test (negative male). Considering the consequences of the result obtained using only the amelogenin marker, and the related potential difficulties in interpreting the results, the gender misinterpretation may be troublesome in clinical practice and in forensic casework. In this article, beginning with a review of the incidence of gender-testing failures among different populations, and with the different strategies proposed in the literature in case of doubt regarding the presence of deleted AMEL in the DNA profile, we propose a method for the identification of samples with deleted AMEL that can be applied, as an additional assay, in case of doubt regarding PCR results of sex determination.
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Amelogenina/genética , Cromosomas Humanos Y , Eliminación de Gen , Análisis para Determinación del Sexo/métodos , Dermatoglifia del ADN , Cartilla de ADN , Electroforesis en Gel de Poliacrilamida , Femenino , Humanos , Masculino , Reacción en Cadena de la PolimerasaRESUMEN
The 2011 collaborative exercise of the ISFG Italian Working Group GeFI was aimed at validating the five ENFSI/EDNAP miniSTR loci D1S1656, D2S441, D10S1248, D12S391 and D22S1045. The protocol required to type at least 50 multilocus profiles from locally resident individuals and two blind bloodstains in duplicate (i.e., using at least two different commercial kits), and to send the electropherograms to the Organizing Committee. Nineteen laboratories distributed across Italy participated, collecting a total of 960 samples. Full concordance was found for the five new miniSTRs as observed from the comparison of 13,150 alleles. The inspection of the electropherograms allowed the identification of a very limited number of mistypings in the miniSTR genotypes thus contributing to the establishment of an high quality Italian database of frequencies.
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Mapeo Cromosómico , Genética de Población , Genética Forense , Humanos , Italia , Laboratorios , Repeticiones de MicrosatéliteRESUMEN
Knowledge of the carrion-breeding insects present at a local level is important and necessary for defining the post-mortem interval. Climate changes and globalisation are affecting species ranges and population dynamics. In this note, we report the incidence of Chrysomya albiceps (Diptera: Calliphoridae) on dead human bodies and carrion in Northern Italy. These data confirm the spread of this species in the Northern regions. The partial sequencing of a 583-bp region of the cytochrome oxidase subunit 1 gene of an Adriatic population did not reveal any difference compared to the same genomic region in the African and South American populations of this species.
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Dípteros/crecimiento & desarrollo , Complejo IV de Transporte de Electrones/genética , Medicina Legal , Adulto , Animales , Cadáver , Dípteros/enzimología , Femenino , Humanos , Italia , Larva/enzimología , Larva/crecimiento & desarrollo , Persona de Mediana Edad , Dinámica Poblacional , PorcinosRESUMEN
Knowledge of the carrion-breeding insects present at a local level is important and necessary for defining the post-mortem interval. Climate changes and globalisation are affecting species ranges and population dynamics. In this note, we report the incidence of Chrysomya albiceps (Diptera: Calliphoridae) on dead human bodies and carrion in Northern Italy. These data confirm the spread of this species in the Northern regions. The partial sequencing of a 583-bp region of the cytochrome oxidase subunit 1 gene of an Adriatic population did not reveal any difference compared to the same genomic region in the African and South American populations of this species.
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Adulto , Animales , Femenino , Humanos , Persona de Mediana Edad , Dípteros/crecimiento & desarrollo , Complejo IV de Transporte de Electrones/genética , Medicina Legal , Cadáver , Dípteros/enzimología , Italia , Larva/enzimología , Larva/crecimiento & desarrollo , Dinámica Poblacional , PorcinosRESUMEN
In Italy, judicial and extrajudicial requests for paternity testing have increased in recent years. A retrospective analysis of such private extrajudicial requests received by the legal medicine unit of the Department of Environmental Medicine and Public Health of Padua University was conducted to identify problem areas most helpful in determining whether to accept private parties' requests for paternity testing. Such testing is most delicate when a presumptive father may be seeking to disown paternity and when testing is wanted without the consent of a member of the mother-child-father triangle. Tests that could establish paternity where none has been recognised are less problematic, as the child will not lose out. Legal and ethical-deontological aspects of consent, of the protection of minors and of children's and parents' need for follow-up interviews to deal with the outcome of such testing are carefully considered by the Padua University team when deciding whether to accept a request for testing. It is argued that because such issues are not dealt with by mail-order laboratories, the use of such services is inappropriate.
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Confidencialidad/ética , Dermatoglifia del ADN/ética , Ilegitimidad/legislación & jurisprudencia , Relaciones Padres-Hijo/legislación & jurisprudencia , Paternidad , Niño , Confidencialidad/legislación & jurisprudencia , Dermatoglifia del ADN/legislación & jurisprudencia , Femenino , Humanos , Ilegitimidad/ética , Lactante , Italia , Masculino , PadresAsunto(s)
Infecciones por Helicobacter/complicaciones , Helicobacter pylori , Interleucina-12/genética , Polimorfismo de Nucleótido Simple , Subunidades de Proteína/genética , Neoplasias Gástricas/genética , Adenocarcinoma/genética , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Predisposición Genética a la Enfermedad , Humanos , Subunidad p35 de la Interleucina-12 , Subunidad p40 de la Interleucina-12 , Masculino , Persona de Mediana Edad , Repeticiones de MinisatéliteRESUMEN
In this study, we describe a pentaplex PCR to determine the parental origin of the X chromosome and the presence of mosaicism, via amplification of four polymorphic markers located along the X chromosome (DXS10011, DXS6807, HUMARA, DXS101) and the X-Y amelogenin marker, in 41 families having a daughter with Turner Syndrome. Our results confirmed the cytogenetic findings and we found that the parental origin of the single X chromosome to be maternal in 84% of cases.
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Cromosomas Humanos X , Cromosomas Humanos Y , Reacción en Cadena de la Polimerasa/métodos , Síndrome de Turner/genética , Adulto , Amelogenina , Niño , Preescolar , Proteínas del Esmalte Dental/genética , Familia , Femenino , Marcadores Genéticos , Humanos , Mosaicismo , Polimorfismo Genético , Aberraciones Cromosómicas SexualesRESUMEN
A sample of 1176 males from 10 Italian regions have been typed for DYS19, DYS389-I, DYS389-II, DYS390, DYS391, DYS392, DYS393, and DYS385. Individual haplotype data are available on line. A low degree of variation is present among regions. Use of this database is specifically recommended for forensic applications in Italy.
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Genética de Población , Haplotipos/genética , Cromosoma Y/genética , Bases de Datos Factuales , Humanos , Italia , MasculinoRESUMEN
Sex determination in both fetuses and infants with ambiguous external genitalia usually necessitates time-consuming and costly karyotyping. We propose a simple, rapid, and reliable method of prenatal and postnatal sex determination by means of the PCR, a technique currently used to identify gender for forensic purposes. DNA was extracted from 20 samples of whole blood from infants with ambiguous genitalia and from five samples of amniotic fluid. Three markers were amplified from each specimen: a Y chromosome alphoid repeat sequence and two homologous genes, amelogenin (AME) and zinc finger protein (ZFP). All three were detected in under 10 hr. A comparison of the results obtained with those of cytogenetic analysis of the same samples showed a perfect sex match, demonstrating that this PCR technique provides an accurate means of determining gender.
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Genitales/anomalías , Reacción en Cadena de la Polimerasa , Diagnóstico Prenatal , Análisis para Determinación del Sexo , Amelogenina , Amniocentesis , Proteínas del Esmalte Dental/genética , Femenino , Humanos , Masculino , Secuencias Repetitivas de Ácidos Nucleicos , Cromosoma Y/genética , Dedos de ZincRESUMEN
Historical studies on the Asiago Plateau have pointed to the peculiarity of its inhabitants in terms of their socio-cultural development marked by long periods of linguistic and cultural isolation. The present research on some serum protein markers (TF, GC and HP) aims to establish whether this isolation may have caused this population to become different from the others in terms of gene frequencies. For this purpose, transferrin (TF), group-specific component (GC) and human haptoglobin (HP) polymorphisms were studied in 435 subjects. GC and HP were found to be within the range of variation known for the Italian Peninsula.
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Alelos , Etnicidad/genética , Frecuencia de los Genes , Haptoglobinas/genética , Transferrina/genética , Proteína de Unión a Vitamina D/genética , Femenino , Humanos , Italia , Masculino , Polimorfismo GenéticoRESUMEN
We determined the precise genetic location of the human endothelin-1 gene (EDN1), which encodes a peptide with extremely potent vasoactive properties and is apparently involved in a spectrum of diseases ranging from hypertension to asthma. Analyzing the segregation of a four-allele EDN1 polymorphism in 40 CEPH families including 480 individuals, we detected significant linkage of EDN1 to DNA markers spanning the telomeric half of chromosome arm 6p. EDN1 was closest to the highly polymorphic nucleotide-repeat marker D6S89 at a theta = 0.06 with the highest pairwise LOD score Zmax = 31.2. Subsequent multipoint analysis placed EDN1 at 8 cM distal to D6S89; EDN1 was flanked at its telomeric site at a 13-cM distance by the gene encoding the A subunit of blood clotting factor XIII (F13A1). Furthermore, EDN1 was located at approximately 34-36 cM distal to the HLA region defined by HLA-A, -B, and -DRB1, and 31 cM proximal to the most telomeric marker D6S7. This location of EDN1 on the primary genetic map is strongly supported with odds of 2.7 x 10(12):1 against the next best alternative.
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Mapeo Cromosómico , Cromosomas Humanos Par 6 , Endotelinas/genética , Southern Blotting , Femenino , Ligamiento Genético , Marcadores Genéticos , Antígenos HLA/genética , Humanos , Escala de Lod , Masculino , Lugares Marcados de Secuencia , TelómeroRESUMEN
A cDNA encoding a G protein-coupled receptor that appears to mediate the behavioral effects of cannabinoids, the psychoactive ingredients of marijuana, has recently been cloned from rat cerebral cortex and expressed. We have now determined the genomic location of the human cannabinoid receptor gene (CNR) by a combination of genetic linkage mapping and chromosomal in situ hybridization. The segregation pattern of a CNR DNA polymorphism was analyzed in 508 individuals from two or three generations of 40 families. Linkage of CNR to chromosome 6 centromeric loci and to DNA markers on the long and short arms was detected. CNR was tightly linked to D6S27, which is known to be located at 6q (log10 odds ratio [lod score, Zmax] of 10.54 at a recombination fraction [theta] of 0.02). Close linkage was suggested between CNR and CGA, the locus for the alpha subunit of human chorionic gonadotropin (Zmax = 2.71 at theta = 0). Moreover, CNR was linked to the two markers 308/BamHI (theta = 0.14) and 308/TaqI (theta = 0.20) defining locus D6Z1, an extended, highly repetitive, and highly conserved sequence localized exclusively to centromeres of all chromosomes and enriched on chromosome 6. In situ hybridization using a biotinylated cosmid probe localizes the gene to 6q14-q15, thereby confirming the linkage analysis and defining a precise alignment of the genetic and cytogenetic maps.