RESUMEN
A simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field. Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1, 4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.
Asunto(s)
Óxido Ferrosoférrico/química , Fructanos/química , Lectinas de Plantas/aislamiento & purificación , Canavalia/química , Cromatografía Liquida , Concanavalina A/aislamiento & purificación , Fabaceae/química , Fructanos/metabolismo , Pruebas de Inhibición de Hemaglutinación , Resonancia Magnética Nuclear Biomolecular , Lectinas de Plantas/metabolismo , Zymomonas/químicaRESUMEN
The effects of inoculon size, centrifuged or not, on the batch alcoholit fermentation by Zymomonas mobilis (strain ZAP) were studied. The results show that the ethanol yeld factor, the "economical" and the biochemical" efficiencies and the productivity increase with the elevation of inoculum independently of the inoculation methodology (centrifuged or not). The specific rates of cells growth (micronx), glucose uptake (microngs) and ethanol production (microngp) decrease with the elevation of inoculum level. It was observed an apparente limitation of growth with the increase above 20% (0,50 g/l) of the uncentrifuged inoculum