RESUMEN
An impacted third molar is one of the most common dental abnormalities. Among the reasons for impaction the most common are: insufficient space, time of eruption, improper position of the tooth bud, and genetic disruptions. To investigate if runt-related transcription factor 2 (RUNX2), bone morphogenetic protein 2 (BMP2), and msh homeobox 1 (MSX1) are differently expressed depending on the position of the molar, we studied 32 patients who had been referred for surgical removal. An orthopantomogram was used to separate them according to Winter's, and Pell & Gregory's, classifications. Bone samples were harvested during the operation for gene expression assay. The Kruskal-Wallis, Dunn's post hoc, and Spearman's correlation, tests were used to assess the significance of differences. No correlations were found in expression of the genes, and no differences between expression in maxillary and mandibular third molars, nor were they expressed differently according to Winter's or Pell and Gregory's classifications or in relation to impaction of the mandibular ramus. However, MSX1 was expressed differently when account was taken of the depth of impaction in maxillary third molars (pâ¯=â¯0.029), but there was no difference in expression of RUNX2, BMP2, and MSX1 for the Pell and Gregory classification of depth of impaction (pâ¯>â¯0.05). We conclude that MSX1 is expressed differently depending on the depth of maxillary impaction phenotypes.
Asunto(s)
Tercer Molar , Diente Impactado , Humanos , Factor de Transcripción MSX1 , Mandíbula , Diente Molar , Erupción DentalRESUMEN
Vaccinia virus (VACV) is an important pathogen. Although studies have shown relationships between probiotics and viruses, the effect of probiotics on VACV infection is unknown. Therefore, this work aims to investigate the probiotics effects on VACV infection. Mice were divided into four groups, two non-infected groups, one receiving the probiotic, the other one not receiving it, and two groups infected intranasally with VACV Western Reserve (VACV-WR) receiving or not receiving the probiotic. Viral titres in organs and cytokine production in the lungs were analysed. Lung samples were also subjected to histological analysis. The intake of probiotic results in reduction in viral spread with a significant decrease of VACV titer on lung, liver and brain of treated group. In addition,treatment with the probiotic results in attenuated mice lung inflammation showing fewer lesions on histological findings and decreased lethality in mice infected with VACV. The ingestion of Lactobacillus paracasei ST11 (LPST11) after VACV infection resulted in 2/9 animal lethality compared with 4/9 in the VACV group. This is the first study on probiotics and VACV interactions, providing not only information about this interaction, but also proposing a model for future studies involving probiotics and other poxvirus.
Asunto(s)
Lacticaseibacillus paracasei/fisiología , Probióticos , Virus Vaccinia/fisiología , Vaccinia/terapia , Animales , Citocinas/análisis , Modelos Animales de Enfermedad , Ingestión de Alimentos , Inflamación/terapia , Pulmón/patología , Pulmón/virología , Masculino , Ratones , Ratones Endogámicos BALB CAsunto(s)
Anemia Aplásica/terapia , Trasplante de Médula Ósea/efectos adversos , Infecciones por Virus de Epstein-Barr/complicaciones , Síndrome de Secreción Inadecuada de ADH/complicaciones , Trastornos Linfoproliferativos/etiología , Adulto , Anticuerpos Monoclonales/administración & dosificación , Anticuerpos Monoclonales de Origen Murino , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Doxorrubicina/administración & dosificación , Femenino , Humanos , Síndrome de Secreción Inadecuada de ADH/tratamiento farmacológico , Trastornos Linfoproliferativos/tratamiento farmacológico , Rituximab , Trasplante Homólogo/efectos adversosAsunto(s)
Anticuerpos Monoclonales/uso terapéutico , Trasplante de Médula Ósea/efectos adversos , Síndrome Hemolítico-Urémico/etiología , Síndrome Hemolítico-Urémico/terapia , Adulto , Anticuerpos Monoclonales de Origen Murino , Síndrome Hemolítico-Urémico/fisiopatología , Humanos , Masculino , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapia , Rituximab , Trasplante HomólogoAsunto(s)
Fungemia/microbiología , Leucemia Mielógena Crónica BCR-ABL Positiva/microbiología , Micosis/microbiología , Pichia/aislamiento & purificación , Anfotericina B/uso terapéutico , Antifúngicos/uso terapéutico , Femenino , Fungemia/tratamiento farmacológico , Humanos , Leucemia Mielógena Crónica BCR-ABL Positiva/tratamiento farmacológico , Persona de Mediana Edad , Micosis/tratamiento farmacológicoAsunto(s)
Anticuerpos Monoclonales/administración & dosificación , Antineoplásicos/administración & dosificación , Linfoma no Hodgkin/tratamiento farmacológico , Diálisis Renal/métodos , Insuficiencia Renal/tratamiento farmacológico , Anciano , Anticuerpos Monoclonales de Origen Murino , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Resultado Fatal , Femenino , Humanos , Linfoma no Hodgkin/complicaciones , Masculino , Persona de Mediana Edad , Inducción de Remisión , Rituximab , Trombosis de la Vena/complicacionesRESUMEN
Peripheral blood progenitor cell (PBPC) harvests mobilized by granulocyte colony-stimulating factor (G-CSF) contain more CD34+ cells and provide more rapid engraftment than do bone marrow (BM) harvests. However, some reports have suggested a higher risk of chronic graft-versus-host disease (GVHD), possibly because such PBPC harvests contain approximately 10 times more T lymphocytes than do BM harvests. Some groups are attempting to combine the faster engraftment of PBPCs with the lower incidence of GVHD observed after BM transplantation by using G-CSF-primed BM conventionally harvested from iliac crests for allogenic BM transplantation. We report the results of a pilot study of 38 allogeneic transplants using G-CSF-stimulated BM from related donors, with a focus on the harvest composition, engraftment, and incidence of acute and chronic GVHDs.
Asunto(s)
Trasplante de Médula Ósea/métodos , Supervivencia de Injerto , Enfermedad Injerto contra Huésped/inmunología , Factor Estimulante de Colonias de Granulocitos/farmacología , Neoplasias Hematológicas/terapia , Células Madre Hematopoyéticas/efectos de los fármacos , Subgrupos de Linfocitos T/efectos de los fármacos , Enfermedad Aguda , Adolescente , Adulto , Trasplante de Médula Ósea/mortalidad , Causas de Muerte , Niño , Preescolar , Enfermedad Crónica , Países en Desarrollo , Femenino , Neoplasias Hematológicas/mortalidad , Células Madre Hematopoyéticas/clasificación , Humanos , Masculino , Persona de Mediana Edad , Proyectos Piloto , Subgrupos de Linfocitos T/clasificación , Trasplante Homólogo , Resultado del TratamientoAsunto(s)
Trasplante de Médula Ósea , Neoplasias del Sistema Nervioso Central/etiología , Efecto Injerto vs Leucemia , Leucemia Promielocítica Aguda/cirugía , Acondicionamiento Pretrasplante/métodos , Adulto , Neoplasias del Sistema Nervioso Central/inmunología , Efecto Injerto vs Leucemia/inmunología , Humanos , Masculino , Recurrencia , Inducción de Remisión , Trasplante HomólogoAsunto(s)
Inmunoglobulinas Intravenosas/uso terapéutico , Linfohistiocitosis Hemofagocítica/etiología , Mieloma Múltiple/complicaciones , Mieloma Múltiple/terapia , Trasplante de Células Madre de Sangre Periférica/efectos adversos , Trasplante Autólogo/efectos adversos , Citocinas/metabolismo , Femenino , Humanos , Sistema Inmunológico/metabolismo , Linfohistiocitosis Hemofagocítica/diagnóstico , Persona de Mediana Edad , Fagocitosis , Factores de Tiempo , Resultado del TratamientoAsunto(s)
Antibacterianos/uso terapéutico , Farmacorresistencia Bacteriana Múltiple , Polimixina B/uso terapéutico , Pseudomonas aeruginosa/efectos de los fármacos , Rifampin/uso terapéutico , Adulto , Celulitis (Flemón)/tratamiento farmacológico , Celulitis (Flemón)/microbiología , Quimioterapia Combinada , Femenino , Humanos , Masculino , Persona de Mediana Edad , Neutropenia , Flebitis/tratamiento farmacológico , Flebitis/microbiología , Infecciones por Pseudomonas/tratamiento farmacológico , Infecciones por Pseudomonas/microbiología , Resultado del TratamientoAsunto(s)
Anemia Aplásica/terapia , Trasplante de Médula Ósea/métodos , Hemofilia A/terapia , Anemia Aplásica/complicaciones , Niño , Preescolar , Factor VIII/genética , Resultado Fatal , Hemofilia A/complicaciones , Prueba de Histocompatibilidad , Humanos , Masculino , Transfusión de Plaquetas , Recurrencia , Hermanos , Acondicionamiento Pretrasplante/métodos , Trasplante HomólogoRESUMEN
The purposes of the present study were: 1) to investigate the biocompatibility of a natural resin (made of fatty acids extracted from Ricinus communis) implanted in the dental alveolus of rats and 2) to verify any possible interference of that material in the osseous healing following tooth extraction. The resin (AUG-EX, Poliquil Araraquara Polímeros Químicos LTDA, Araraquara--SP) was placed inside de alveoli immediately after extraction of the upper right incisors. The animals were sacrificed 1, 2, 3 and 6 weeks after extraction or extraction + implantation. The hemi-maxillae were decalcified and processed for paraffin embedding. Longitudinal 6-micrometer-thick semi-serial sections stained with hematoxylin and eosin were obtained. Histologic examination showed particles of irregular shape and variable size (700-1200 microns) localized in the medium/cervical alveolar thirds, with a scanty but persistent foreign body reaction. From the second week on, as the relative volume of bone trabeculae increased, it was seen in close contact with the surface of the implanted material in some regions. Histometric analysis (differential point counting method), used to quantify the healing process in the apical third, showed a small but significant decrease (13%-20%) in new bone formation in the implanted rats. In conclusion, the results show that, in spite of its biocompatible nature, the studied resin hinders the post-extration healing process.