RESUMEN
This study aimed to evaluate the effect of different concentrations of mangabeira latex (Hancorniaspeciosa) added to ram semen extender on motility of cooled semen stored for 24 hours. The latex was added tocitrate-glucose-yolk extender in concentrations of 0% (control), 2%, 4% and 6% (v:v) and diluted with SantaInês ram semen and cooled to 4°C for 24h. The semen was evaluated for sperm motility and vigor. No statisticaldifferences were found for fresh semen and the treatments with the addition of latex. These results indicate thatno improvement in ram semen quality cooled and stored for 24 by the addition of mangabeira latex.(AU)
Asunto(s)
Animales , Masculino , Ovinos/embriología , Apocynaceae/química , Criopreservación/métodos , Criopreservación/veterinaria , AntioxidantesRESUMEN
The objective of this study was to evaluate the ImageJ software in automatic analysis of morphometricparameters of ram sperm head. Semen smears from three Santa Inês rams were stained with fast panoptic anddigital images of sperms from these smears were analyzed in ImageJ software for morphometric parameters.The mean dimensions of sperm heads analyzed were 8,4±0,6 µm; 4,4±0,3 µm; 21,8±1,6 µm and 29,0±3,9 µm2for length, width, perimeter and area, respectively. Individual variations for these parameters were observed.The ImageJ software was suitable for automatic sperm morphometric analysis with results consistent with thosefound in the literature.(AU)
Asunto(s)
Animales , Masculino , Ovinos/fisiología , Espermatozoides/citología , Espermatozoides/fisiología , Diagnóstico por Imagen/métodos , Diagnóstico por Imagen/veterinariaRESUMEN
This study aimed to evaluate the effect of different concentrations of mangabeira latex (Hancorniaspeciosa) added to ram semen extender on motility of cooled semen stored for 24 hours. The latex was added tocitrate-glucose-yolk extender in concentrations of 0% (control), 2%, 4% and 6% (v:v) and diluted with SantaInês ram semen and cooled to 4°C for 24h. The semen was evaluated for sperm motility and vigor. No statisticaldifferences were found for fresh semen and the treatments with the addition of latex. These results indicate thatno improvement in ram semen quality cooled and stored for 24 by the addition of mangabeira latex.
Asunto(s)
Masculino , Animales , Apocynaceae/química , Criopreservación/métodos , Criopreservación/veterinaria , Ovinos/embriología , AntioxidantesRESUMEN
The objective of this study was to evaluate the ImageJ software in automatic analysis of morphometricparameters of ram sperm head. Semen smears from three Santa Inês rams were stained with fast panoptic anddigital images of sperms from these smears were analyzed in ImageJ software for morphometric parameters.The mean dimensions of sperm heads analyzed were 8,4±0,6 µm; 4,4±0,3 µm; 21,8±1,6 µm and 29,0±3,9 µm2for length, width, perimeter and area, respectively. Individual variations for these parameters were observed.The ImageJ software was suitable for automatic sperm morphometric analysis with results consistent with thosefound in the literature.
Asunto(s)
Masculino , Animales , Diagnóstico por Imagen/métodos , Diagnóstico por Imagen/veterinaria , Espermatozoides/citología , Espermatozoides/fisiología , Ovinos/fisiologíaRESUMEN
Leptospirosis is a zoonotic disease which is a major public health problem, particularly in tropical and subtropical countries. This study aimed to investigate the presence of agglutinins anti-Leptospira dogs in the city of Bom Jesus, PI, using the agglutination test Microscopic Technique (SAM). Of the 106 samples, 20 (18.84%) were reactive. Of the 21 evaluated the following serovars were detected: Pyrogenes (43.47%), Canícola (39.13%), Icterohaemorrhagiae (8.69%) and Autumnalis (8.69%). It was concluded that leptospirosis is present in dogs in the city of Bom Jesus, PI, and the serovar most frequent was the Pyrogenes.(AU)
Asunto(s)
Animales , Perros , Aglutininas/análisis , Leptospira/inmunología , Perros/microbiología , Investigación/educaciónRESUMEN
Leptospirosis is a zoonotic disease which is a major public health problem, particularly in tropical and subtropical countries. This study aimed to investigate the presence of agglutinins anti-Leptospira dogs in the city of Bom Jesus, PI, using the agglutination test Microscopic Technique (SAM). Of the 106 samples, 20 (18.84%) were reactive. Of the 21 evaluated the following serovars were detected: Pyrogenes (43.47%), Canícola (39.13%), Icterohaemorrhagiae (8.69%) and Autumnalis (8.69%). It was concluded that leptospirosis is present in dogs in the city of Bom Jesus, PI, and the serovar most frequent was the Pyrogenes.