RESUMEN
The influence of cell differentiation and proliferation on cationic vector mediated gene transfer into the explant-outgrowth cell culture from nasal polyps was investigated. Respiratory cells were categorized into two groups based on the expression of cytokeratin filaments (CKs), which were used as differentiation markers. Outgrowths grown for 2 weeks expressed similar levels of CKs 14, 13 and 18 showing a de-differentiated phenotype, while outgrowths cultured for 4 weeks presented very high levels of CK 13, high CK 14 and low CK 18 expression and were squamous differentiated. De-differentiated cells presented higher proliferation indexes than squamous cells. Gene transfer levels, as evaluated using a quantitative reporter gene (firefly luciferase), were significantly higher in the 2- than in the 4-week-old outgrowths. Cationic vector transfected respiratory cells were located both proximally and distally to the explant, as shown by enzymatic staining of beta-galactosidase-positive cells. Respiratory cell outgrowths from nasal polyps can be considered a suitable model to study gene transfer protocols in vitro.
Asunto(s)
Técnicas de Transferencia de Gen , Vectores Genéticos , Pólipos Nasales/patología , Biomarcadores/análisis , Diferenciación Celular , Células Cultivadas , Fibrosis Quística/patología , Ácidos Grasos Monoinsaturados , Genes Reporteros/genética , Humanos , Queratinas/análisis , Fenotipo , Compuestos de Amonio Cuaternario , TransfecciónRESUMEN
A fluorescent derivative of a chimeric toxin between human pro-urokinase and the plant ribosome-inactivating protein saporin (p-uPA-Sap(TRITC)), has been prepared in order to study the endocytosis of this potentially antimetastatic conjugate in the murine model cell line LB6 clone19 (Cl19) transfected with the human urokinase receptor gene. The physiological internalization of urokinase-inhibitor complexes is triggered by the interaction of plasminogen inhibitors (PAIs) with receptors belonging to the low density lipoprotein-related receptor protein (LRP) family, and involves a macro-quaternary structure including uPAR, LRP, and PAIs. However, in contrast to this mechanism, we observed a two-step process: first, the urokinase receptor (uPAR) acts as the anchoring factor on the plasma membrane; subsequently, LRP acts as the endocytic trigger. Once the chimera is bound to the plasma membrane by interaction with uPAR, we suggest that a possible exchange may occur to transfer the toxin to LRP via the saporin moiety and begin the internalization. So an unusual endocytic process is described, where the toxin enters the cell via a receptor different from that used to bind the plasma membrane.
Asunto(s)
Inmunotoxinas , N-Glicosil Hidrolasas , Proteínas de Plantas/metabolismo , Activador de Plasminógeno de Tipo Uroquinasa/metabolismo , Animales , Muerte Celular/efectos de los fármacos , Membrana Celular/metabolismo , Clorpromazina/farmacología , Células Clonales , Endocitosis , Filipina/farmacología , Colorantes Fluorescentes , Humanos , Inmunohistoquímica , Proteína 1 Relacionada con Receptor de Lipoproteína de Baja Densidad , Ratones , Microscopía Fluorescente , Microscopía Inmunoelectrónica , Proteínas de Plantas/toxicidad , Inactivadores Plasminogénicos/metabolismo , Receptores de Superficie Celular/metabolismo , Receptores Inmunológicos/metabolismo , Receptores del Activador de Plasminógeno Tipo Uroquinasa , Proteínas Recombinantes de Fusión/metabolismo , Proteínas Recombinantes de Fusión/toxicidad , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/toxicidad , Proteínas Inactivadoras de Ribosomas Tipo 1 , Saporinas , Activador de Plasminógeno de Tipo Uroquinasa/toxicidadRESUMEN
Human bronchial epithelial cells were treated in vitro with interferon-gamma or tumor necrosis factor-alpha to assess their effect on transepithelial ion transport. Short-circuit current measurements revealed that Na(+) absorption was markedly inhibited by interferon-gamma (10-1,000 U/ml). The cystic fibrosis transmembrane conductance regulator was also downregulated by interferon-gamma as evident at the protein level and by the decrease in the cAMP-dependent current. On the other hand, interferon-gamma caused an increase of the current elicited by apical UTP application, which is due to the activity of Ca(2+)-dependent Cl(-) channels. Tumor necrosis factor-alpha caused few changes in ion transport. Transepithelial fluid transport was measured in normal and cystic fibrosis cells. At rest, both types of cells showed an amiloride-sensitive fluid absorption that was inhibited by interferon-gamma but not by tumor necrosis factor-alpha. Our results show that interferon-gamma alters the transepithelial ion transport of cultured bronchial cells. This effect may change the ion composition and/or volume of periciliary fluid.
Asunto(s)
Bronquios/metabolismo , Interferón gamma/farmacología , Transporte Biológico/efectos de los fármacos , Líquidos Corporales/metabolismo , Bronquios/citología , Bronquios/efectos de los fármacos , Calcio/metabolismo , Membrana Celular/efectos de los fármacos , Células Cultivadas , Canales de Cloruro/efectos de los fármacos , Canales de Cloruro/fisiología , AMP Cíclico/fisiología , Fibrosis Quística/metabolismo , Fibrosis Quística/patología , Regulador de Conductancia de Transmembrana de Fibrosis Quística/metabolismo , Regulación hacia Abajo , Conductividad Eléctrica , Células Epiteliales/efectos de los fármacos , Células Epiteliales/metabolismo , Humanos , Iones , Valores de Referencia , Canales de Sodio/efectos de los fármacos , Canales de Sodio/fisiología , Factor de Necrosis Tumoral alfa/farmacología , Uridina Trifosfato/farmacologíaRESUMEN
In this work, we have devised an intracellular immunization strategy for the expression in high amounts of ATF-saporin, a targeted chimeric toxin constituted by the ATF receptor binding domain of human urokinase and the plant ribosome-inactivating protein saporin, which has been shown to be highly cytotoxic to target cells. This strategy may allow the production of highly toxic secretory proteins in eukaryotic cells, avoiding cell suicide caused by autointoxication. The procedure consists of equipping host cells with cytosolic neutralizing antibodies directed toward the toxic domain of the heterologous polypeptide. We show that this intracellular immunization is essential for the synthesis of correctly folded, biologically active ATF-SAP in the high amounts needed to investigate its in vivo anti-metastatic potential. Such a strategy should be generally useful for the production of toxic molecules of therapeutic value whose folding and maturation require transit through the eukaryotic secretory pathway. Fabbrini, M. S., Carpani, D., Soria, M. R., Ceriotti, A. Cytosolic immunization allows the expression of preATF-saporin chimeric toxin in eukaryotic cells.
Asunto(s)
Inmunotoxinas/metabolismo , Proteínas de Plantas/biosíntesis , Precursores de Proteínas/biosíntesis , Proteínas Recombinantes de Fusión , Proteínas Recombinantes de Fusión/biosíntesis , Activador de Plasminógeno de Tipo Uroquinasa/biosíntesis , Animales , Anticuerpos/farmacología , Citosol , Células Eucariotas , Humanos , Inmunotoxinas/inmunología , N-Glicosil Hidrolasas , Oocitos , Proteínas de Plantas/inmunología , Receptores de Superficie Celular/metabolismo , Receptores del Activador de Plasminógeno Tipo Uroquinasa , Proteínas Recombinantes de Fusión/inmunología , Proteínas Recombinantes/biosíntesis , Células U937 , Activador de Plasminógeno de Tipo Uroquinasa/inmunología , XenopusRESUMEN
In contrast to two-chain urokinase (uPA), a chemical conjugate between uPA and native saporin (a cytotoxic plant seed ribosome-inactivating protein) did not require plasminogen activator inhibitors to be internalized. To dissect this pathway, we constructed a chimera consisting of the amino-terminal fragment (ATF) of human urokinase fused to a saporin isoform (SAP-3). The chimeric ATF-SAP toxin was expressed in Escherichia coli, purified, and characterized for its ribosome-inactivating activity. Besides being a potent inhibitor of protein synthesis in cell-free assays, ATF-SAP was specifically cytotoxic toward cells expressing human uPAR. Competition experiments indicated that both the human uPAR and the LDL-related receptor protein are involved in mediating the cell killing ability of ATF-SAP. We conclude that neither plasminogen activator inhibitors nor the catalytic moiety of urokinase are necessary to initiate these internalization pathways. Thus, saporin may play a role similar to plasminogen activator inhibitors in its ability to trigger internalization of uPAR-bound ligands through endocytic receptors.
Asunto(s)
Inmunotoxinas/farmacocinética , N-Glicosil Hidrolasas , Fragmentos de Péptidos/farmacocinética , Proteínas de Plantas/farmacocinética , Proteínas Recombinantes de Fusión , Activador de Plasminógeno de Tipo Uroquinasa/farmacocinética , Línea Celular , Supervivencia Celular/efectos de los fármacos , Clonación Molecular , Escherichia coli , Humanos , Inmunotoxinas/toxicidad , Fragmentos de Péptidos/toxicidad , Proteínas de Plantas/toxicidad , Inhibidores de la Síntesis de la Proteína/farmacocinética , Inhibidores de la Síntesis de la Proteína/toxicidad , Receptores de Superficie Celular/biosíntesis , Receptores de Superficie Celular/fisiología , Receptores del Activador de Plasminógeno Tipo Uroquinasa , Proteínas Recombinantes/farmacocinética , Proteínas Inactivadoras de Ribosomas Tipo 1 , Ribosomas/efectos de los fármacos , Saporinas , Células Tumorales CultivadasRESUMEN
We have expressed in Escherichia coli five isoforms of saporin, a single-chain ribosome-inactivating protein (RIP). Translation inhibition activities of the purified recombinant polypeptides in vitro were compared with those of recombinant dianthin 30, a less potent and closely related RIP, and of ricin A chain. Dianthin 30, and a saporin isoform encoded by a cDNA from leaf tissue (SAP-C), both had about one order of magnitude lower activity in translation inhibition assays than all other isoforms of saporin tested. We recently demonstrated that saporin extracted from seeds of Saponaria officinalis binds to alpha2-macroglobulin receptor (alpha2MR; also termed low density lipoprotein-receptor-related-protein), indicating a general mechanism of interaction of plant RIPs with the alpha2MR system [Cavallaro, Nykjaer, Nielsen and Soria (1995) Eur. J. Biochem. 232, 165-171]. Here we report that SAP-C bound to alpha2MR equally well as native saporin. However, the same isoform had about ten times lower cytotoxicity than the other saporin isoforms towards different cell lines. This indicates that the lower cell-killing ability of the SAP-C isoform is presumably due to its altered interaction with the protein synthesis machinery of target cells. Since saporin binding to the alpha2MR is competed by heparin, we also tested in cell-killing experiments Chinese hamster ovary cell lines defective for expression of either heparan sulphates or proteoglycans. No differences were observed in cytotoxicity using native saporin or the recombinant isoforms. Therefore saporin binding to the cell surface should not be mediated by interaction with proteoglycans, as is the case for other alpha2MR ligands.