RESUMEN
Recently, a novel mode of inheritance has been described in the yeast Saccharomyces cerevisiae. The mechanism is based on the prion hypothesis, which posits that self-perpetuating changes in the conformation of single protein, PrP, underlie the severe neurodegeneration associated with the transmissible spongiform enchephalopathies in mammals. In yeast, two prions, [URE3] and [PSI+], have been identified, but these factors confer unique phenotypes rather than disease to the organism. In each case, the prion-associated phenotype has been linked to alternative conformations of the Ure2 and Sup35 proteins. Remarkably, Ure2 and Sup35 proteins existing in the alternative conformations have the unique capacity to transmit this physical state to the newly synthesized protein in vivo. Thus, a mechanism exists to ensure replication of the conformational information that underlies protein-only inheritance. We have characterized the mechanism by which Sup35 conformational information is replicated in vitro. The assembly of amyloid fibres by a region of Sup35 encompassing the N-terminal 254 amino acids faithfully recapitulates the in vivo propagation of [PSI+]. Mutations that alter [PSI+] inheritance in vivo change the kinetics of amyloid assembly in vitro in a complementary fashion, and lysates from [PSI+] cells, but not [psi-] cells, accelerate assembly in vitro. Using this system we propose a mechanism by which the alternative conformation of Sup35 is adopted by an unstructured oilgomeric intermediate at the time of assembly.
Asunto(s)
Proteínas Fúngicas/química , Proteínas Fúngicas/genética , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/química , Saccharomyces cerevisiae/genética , Amiloide/química , Modelos Moleculares , Factores de Terminación de Péptidos , Priones/química , Priones/genética , Conformación Proteica , Pliegue de Proteína , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/genéticaRESUMEN
Prion proteins can serve as genetic elements by adopting distinct physical and functional states that are self-perpetuating and heritable. The critical region of one prion protein, Sup35, is initially unstructured in solution and then forms self-seeded amyloid fibers. We examined in vitro the mechanism by which this state is attained and replicated. Structurally fluid oligomeric complexes appear to be crucial intermediates in de novo amyloid nucleus formation. Rapid assembly ensues when these complexes conformationally convert upon association with nuclei. This model for replicating protein-based genetic information, nucleated conformational conversion, may be applicable to other protein assembly processes.
Asunto(s)
Amiloide/química , Proteínas Fúngicas/química , Priones/química , Proteínas de Saccharomyces cerevisiae , Biopolímeros/química , Centrifugación por Gradiente de Densidad , Dicroismo Circular , Electroforesis en Gel de Poliacrilamida , Endopeptidasas/metabolismo , Proteínas Fúngicas/metabolismo , Proteínas Fúngicas/ultraestructura , Cinética , Luz , Micelas , Microscopía de Fuerza Atómica , Microscopía Electrónica , Modelos Químicos , Factores de Terminación de Péptidos , Priones/metabolismo , Priones/ultraestructura , Conformación Proteica , Pliegue de Proteína , Dispersión de Radiación , Solubilidad , SonicaciónAsunto(s)
Proteínas Fúngicas/química , Priones/química , Proteínas de Saccharomyces cerevisiae , Centrifugación/métodos , Rojo Congo/química , Escherichia coli , Proteínas Fúngicas/genética , Proteínas Fúngicas/ultraestructura , Sustancias Macromoleculares , Microscopía Electrónica/métodos , Factores de Terminación de Péptidos , Plásmidos/genética , Priones/genética , Priones/ultraestructura , Proteínas Recombinantes de Fusión/biosíntesis , Saccharomyces cerevisiae/química , Dodecil Sulfato de Sodio/químicaRESUMEN
Phosphatases are known to play a crucial role in phosphate turnover in plants. However, the exact role of acid phosphatases in plants has been elusive because of insufficient knowledge of their in vivo substrate and subcellular localization. We investigated the biochemical properties of a purple acid phosphatase isolated from red kidney bean (Phaseolus vulgaris) (KBPAP) with respect to its substrate and inhibitor profiles. The kinetic parameters were estimated for five substrates. We used 31P nuclear magnetic resonance to investigate the in vivo substrate of KBPAP. Chemical and enzymological estimation of polyphosphates and ATP, respectively, indicated the absence of polyphosphates and the presence of ATP in trace amounts in the seed extracts. Immunolocalization using antibodies raised against KBPAP was unsuccessful because of the non-specificity of the antiserum toward glycoproteins. Using histoenzymological methods with ATP as a substrate, we could localize KBPAP exclusively in the cell walls of the peripheral two to three rows of cells in the cotyledons. KBPAP activity was not detected in the embryo. In vitro experiments indicated that pectin, a major component of the cell wall, significantly altered the kinetic properties of KBPAP. The substrate profile and localization suggest that KBPAP may have a role in mobilizing organic phosphates in the soil during germination.
RESUMEN
Quantitative equilibrium denaturation studies on oligomeric proteins have the potential to provide information on the role of subunit interactions in protein function and structure. We studied the equilibrium denaturation of red kidney bean purple acid phosphatase (KBPAP), a homodimer with a single disulfide bond between the two subunits, with an objective to understand the role of the intersubunit disulfide bond in KBPAP structure. Binding of 8-anilino-1-naphthalenesulfonic acid, enzymatic activity, size-exclusion chromatography, tryptophan fluorescence and circular dichroism studies revealed that the protein undergoes unfolding through at least three intermediates. Susceptibility of KBPAP for denaturation increases on reduction of the disulfide and aggregation was the predominant product of denaturation. In terms of stability, an intersubunit disulfide bond contributes to 25% of the overall stability of the dimer.
Asunto(s)
Fosfatasa Ácida/química , Disulfuros/química , Glicoproteínas/química , Naftalenosulfonatos de Anilina/química , Cromatografía en Gel/métodos , Dicroismo Circular , Estabilidad de Enzimas , Fabaceae/enzimología , Guanidina , Guanidinas/química , Plantas Medicinales , Desnaturalización Proteica , Pliegue de Proteína , Espectrometría de Fluorescencia , Triptófano/químicaRESUMEN
Structural basis for ligand-induced protein stabilization was investigated in the case of an acid phosphatase (red kidney bean purple acid phosphatase (KBPAP)) from red kidney bean. Phosphate, a physiological ligand, increases the stability against solvent denaturation by 3.5 kcal/mol. Generality of phosphate stabilization was shown by similar effects with other KBPAP ligands viz. adenosine 5'-O-(thiotriphosphate), a nonhydrolyzable ligand, and arsenate, an inhibitor. The dissociation constant of phosphate obtained from denaturation curves matches with the dissociation constant estimated by conventional methods. The guanidinium chloride-mediated denaturation of KBPAP was monitored by several structural and functional parameters viz. activity, tryptophan fluorescence, 8-anilinonaphthalene 1-sulfonic acid binding, circular dichroism, and size exclusion chromatography, in the presence and absence of 10 mm phosphate. In the presence of phosphate, profiles of all the parameters shift to a higher guanidinium chloride concentration. Noncoincidence of these profiles in the absence of phosphate indicates multistate unfolding pathway for KBPAP; however, in the presence of phosphate, KBPAP unfolds with a single intermediate. Based on the crystal structure, we propose that the Arg258 may have an important role to play in stabilization mediated by phosphate.
Asunto(s)
Fosfatasa Ácida/química , Fosfatasa Ácida/metabolismo , Fabaceae/enzimología , Plantas Medicinales , Conformación Proteica , Adenosina Trifosfato/análogos & derivados , Adenosina Trifosfato/farmacología , Dicroismo Circular , Colorantes Fluorescentes , Guanidina , Guanidinas/farmacología , Cinética , Ligandos , Mercaptoetanol/farmacología , Fosfatos/farmacología , Yoduro de Potasio , Conformación Proteica/efectos de los fármacos , Desnaturalización Proteica , Pliegue de Proteína , Espectrometría de Fluorescencia , TriptófanoRESUMEN
Purple acid phosphatase from red kidney beans (Phaseolus vulgaris) has been purified to homogeneity and characterized. The enzyme is a homodimer of 60 kDa subunits each containing one atom of zinc and iron in the active site. Circular dichroism spectral studies on the purified enzyme reveals that a large portion of the peptide backbone is in the unordered and beta-turn conformation. A unique feature of the red kidney bean acid phosphatase, which we have found, is that one of the two cysteines of each subunit is involved in the formation of an inter-subunit disulphide. The thiol group of the other cysteine is not necessary for the activity of the enzyme. Western blot analysis with antibodies raised against kidney bean acid phosphatase could not recognize acid phosphatases from other sources except from potato. This paper emphasizes the fact that acid phosphatases are functionally, but not structurally, conserved enzymes.