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Carbohydr Res ; 339(2): 335-47, 2004 Jan 22.
Artículo en Inglés | MEDLINE | ID: mdl-14698892

RESUMEN

The sulfated agaran isolated by water extraction from the red seaweed, Acanthophora spicifera (Rhodomelaceae, Ceramiales), is made up of A-units highly substituted with sulfate groups on C-2 (28-30%), sulfates on C-2 and 4,6-O-(1'-carboxyethylidene) groups (9-15%), and only the C-2 sulfate groups (5-8%) with small amounts of C-6 sulfate, 6-O-methyl, and nonsubstituted residues. B-units are formed mainly by 3,6-anhydro-alpha-L-galactose (15-16%) and its precursor, alpha-L-galactose 6-sulfate (10-17%), together with lesser amounts of 3,6-anhydro-alpha-L-galactose 2-sulfate, alpha-L-galactose 2,6-disulfate, alpha-L-galactose 2,3,6-tri-sulfate, alpha-L-galactose 2,6-disulfate 3-xylose, 2-O-methyl-alpha-L-galactose, and unsubstituted alpha-L-galactose. Small, but significant quantities of beta-D-xylose were found in all the fractions, together with small amounts to traces of D-glucose. Some of the fractions have high antiviral activity. Attempts to correlate structure and antiviral activity in agarans are presented.


Asunto(s)
Agar/química , Antivirales/química , Antivirales/farmacología , Rhodophyta/química , Sulfatos/química , Sulfatos/farmacología , Animales , Anticoagulantes/química , Anticoagulantes/farmacología , Conformación de Carbohidratos , Secuencia de Carbohidratos , Supervivencia Celular/efectos de los fármacos , Chlorocebus aethiops , Cromatografía por Intercambio Iónico , Concentración 50 Inhibidora , Datos de Secuencia Molecular , Estructura Molecular , Espectrometría de Masa por Ionización de Electrospray , Relación Estructura-Actividad , Células Vero , Ensayo de Placa Viral
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