The transported cations impose differences in the thermostability of the gastric H,K-ATPase. A kinetic analysis.

This work analyses the thermostability of a membrane protein, the gastric H,K-ATPase, by means of a detailed kinetic characterization of its inactivation process, which showed to exhibit first-order kinetics. We observed parallel time courses for the decrease of ATPase activity, the decrease of the autophosphorylation capacity and the loss of tertiary structure at 49 °C. Higher temperatures were required to induce a significant change in secondary structure. The correspondence between the kinetics of Trp fluorescence measured at 49 °C and the decrease of the residual activity after heating at that temperature, proves the irreversibility of the inactivation process. Inactivation proceeds at different rates in E1 or E2 conformations. The K+-induced E2 state exhibits a lower inactivation rate; the specific effect is exerted with a K0.5 similar to that found at 25 °C, providing a further inkling that K+ occlusion by the H,K-ATPase is not really favoured. Increasing [H+] from pH 8 to pH 7, which possibly shifts the protein to E1, produces a subtle destabilizing effect on the H,K-ATPase. We performed a prediction of potential intramolecular interactions and found that the differential stability between E1 and E2 may be mainly explained by the higher number of hydrophobic interactions in the α- and ß-subunits of E2 conformation.

The interaction of Na+, K+, and phosphate with the gastric H,K-ATPase. Kinetics of E1-E2 conformational changes assessed by eosin fluorescence measurements.

H,K-ATPase and Na,K-ATPase show the highest degree of sequence similarity among all other members of the P-type ATPases family. To explore their common features in terms of ligand binding, we evaluated conformational transitions due to the binding of Na+, K+ and Pi in the H,K-ATPase, and compared the results with those obtained for the Na,K-ATPase. This work shows that eosin fluorescence time courses provide a reasonably precise method to study the kinetics of the E1-E2 conformational changes in the H,K-ATPase. We found that, although Na+ shifts the equilibrium toward the E1 conformation and seems to compete with H+ in ATPase activity assays, it was neither possible to isolate a Na+-occluded state, nor to reveal an influx of Na+ related to H,K-ATPase activity. The high rate of the E2K â†’ E1 transition found for the H,K-ATPase, which is not compatible with the presence of a K+-occluded form, agrees with the negligible level of occluded Rb+ (used as a K+ congener) found in the absence of added ligands. The use of vanadate and fluorinated metals to induce E2P-like states increased the level of occluded Rb+ and suggests that-during dephosphorylation-the probability of K+ to remain occluded increases from the E2P-ground to the E2P-product state. From kinetic experiments we found an unexpected increase in the values of kobs for E2P formation with [Pi]; consequently, to obey the Albers-Post model, the binding of Pi to the E2 state cannot be a rapid-equilibrium reaction.

[Usefulness of frozen section exams during radical cystectomy for urothelial carcinoma]. / Utilité des examens extemporanés lors de la cystectomie totale pour carcinome urothélial.

AIM: To determine the usefulness of the frozen section exams of lymph nodes dissection, ureteral and urethral section during radical cystectomy for urothelial carcinoma and define the impact on the surgical procedure. METHOD: A retrospective, single-center study of data collected from 182 patients who underwent radical cystectomy for an cT=3bN0M0 urothelial bladder cancer between 2016 and 2018. Bladder cancer extension was determined by thoracoabdominal CT with contrast enhancement and urography and an 18-FDG PET scanner. No patient received neoadjuvant chemotherapy. The diagnostic performance of the frozen section exams was related to final examinations. The impact of the result on the initial intervention was determined. RESULTS: The frozen section were positive in 29 lymph nodes dissections (15.9 %), 59 (16.6 %) ureteral and 20 (10.9 %) ureteral recessions. With lymph nodes exams, sensitivity, specificity and positive and negative predictive values were 93.5 %, 100 %, 100 %, and 98.7 %, respectively. With ureteral sections exams the same values were 91.5 %, 100 %, 100 %, and 98.4 % respectively. With urethral section exams, all the values were of 100 %. Finally, all the procedure has been modified for all patients with positive frozen section exam except one positive urethral section that did not give rise to radical urethrectomy. CONCLUSION: Frozen section exams were useful to the urologist during radical cystectomy for urothelial carcinoma. The performances of the frozen section exams carried out were excellent. The information of the urologist of the positive frozen section leeds to modify its management during the intervention in all the studied cases with the exception of one case.

Noiseless loss suppression in quantum optical communication.

We propose a protocol for conditional suppression of losses in direct quantum state transmission over a lossy quantum channel. The method works by noiselessly attenuating the input state prior to transmission through a lossy channel followed by noiseless amplification of the output state. The procedure does not add any noise; hence, it keeps quantum coherence. We experimentally demonstrate it in the subspace spanned by vacuum and single-photon states, and consider its general applicability.

Experimentally feasible quantum erasure-correcting code for continuous variables.

We devise a scheme that protects quantum coherent states of light from probabilistic losses, thus achieving the first continuous-variable quantum erasure-correcting code. If the occurrence of erasures can be probed, then the decoder enables, in principle, a perfect recovery of the original light states. Otherwise, if supplemented with postselection based on homodyne detection, this code can be turned into an efficient erasure-filtration scheme. The experimental feasibility of the proposed protocol is carefully addressed.

Superiority of entangled measurements over all local strategies for the estimation of product coherent states.

It is shown that the ensemble {P(alpha),|alpha|alpha;{*}}, where P(alpha) is a Gaussian distribution of finite variance and |alpha is a coherent state, can be better discriminated with an entangled measurement than with any local strategy supplemented by classical communication. Although this ensemble consists of products of quasiclassical states without any squeezing, it thus exhibits a purely quantum feature. This remarkable effect is demonstrated experimentally by implementing the optimal local strategy on coherent states of light together with a global strategy that yields a higher fidelity.

Non-Gaussian cloning of quantum coherent states is optimal.

We consider the optimal cloning of quantum coherent states with single-clone and joint fidelity as figures of merit. While the latter is maximized by a Gaussian cloner, the former is not: the optimal single-clone fidelity for a symmetric 1-to-2 cloner is 0.6826, compared to 2/3 in a Gaussian setting. This cloner can be realized with an optical parametric amplifier and certain non-Gaussian bimodal states. Finally, we show that the single-clone fidelity of the optimal 1-to-infinity cloner is 1/2. It is achieved by a Gaussian scheme and cannot be surpassed even with supplemental bound entangled states.

Separating the classical and quantum information via quantum cloning.

An application of quantum cloning to optimally interface a quantum system with a classical observer is presented; in particular, we describe a procedure to perform a minimal disturbance measurement on a single qubit by adopting a 1-->2 cloning machine followed by a generalized measurement on a single clone and the anticlone or on the two clones. Such a scheme can be applied to enhance the transmission fidelity over a lossy quantum channel.

Simulating maximal quantum entanglement without communication.

It is known that all causal correlations between two parties which output each 1 bit, a and b, when receiving each 1 bit, x and y, can be expressed as convex combinations of local correlations (i.e., correlations that can be simulated with local random variables) and nonlocal correlations of the form a+b=xy mod 2. We show that a single instance of the latter elementary nonlocal correlation suffices to simulate exactly all possible projective measurements that can be performed on a maximally entangled state of two qubits, with no communication needed at all. This elementary nonlocal correlation thus defines some unit of nonlocality, which we call a nl bit.