RESUMEN
Nanothermometers enable the detection of temperature changes at the microscopic scale, which is crucial for elucidating biological mechanisms and guiding treatment strategies. However, temperature monitoring of micron-scale structures in vivo using luminescent nanothermometers remains challenging, primarily due to the severe scattering effect of biological tissue that compromises the imaging resolution. Herein, a lanthanide luminescence nanothermometer with a working wavelength beyond 1500 nm is developed to achieve high-resolution temperature imaging in vivo. The energy transfer between lanthanide ions (Er3+ and Yb3+) and H2O molecules, called the environment quenching assisted downshifting process, is utilized to establish temperature-sensitive emissions at 1550 and 980 nm. Using an optimized thin active shell doped with Yb3+ ions, the nanothermometer's thermal sensitivity and the 1550 nm emission intensity are enhanced by modulating the environment quenching assisted downshifting process. Consequently, minimally invasive temperature imaging of the cerebrovascular system in mice with an imaging resolution of nearly 200 µm is achieved using the nanothermometer. This work points to a method for high-resolution temperature imaging of micron-level structures in vivo, potentially giving insights into research in temperature sensing, disease diagnosis, and treatment development.
Asunto(s)
Elementos de la Serie de los Lantanoides , Animales , Ratones , Elementos de la Serie de los Lantanoides/química , Temperatura , Luminiscencia , Diagnóstico por Imagen , IonesRESUMEN
RATIONALE: Primary hepatic mucosa-associated lymphoid tissue (MALT) lymphoma is a rare malignant primary hepatic lymphoma. The sensible choice of treatment for patients with primary lymphoma combined with atrial fibrillation (AF) is controversial and challenging. PATIENT CONCERNS: The patient presented with both primary hepatic MALT lymphoma and AF, which was difficult to manage. DIAGNOSES: Pathological and immunohistochemical examination are helpful for definitive diagnosis. INTERVENTIONS: Surgical resection and subsequent anticoagulant therapy are main treatment methods, and adjuvant therapy depends on the situation. OUTCOMES: Primary hepatic MALT lymphoma is easy to misdiagnosis due to a lack of typical symptoms and imaging signs. LESSONS: This case highlights for patients with primary hepatic MALT lymphoma combined with AF, toxicity caused by adjuvant chemotherapy should be fully considered, and careful selection should be made based on the general conditions and complications of patients.
Asunto(s)
Fibrilación Atrial , Linfoma de Células B de la Zona Marginal , Humanos , Linfoma de Células B de la Zona Marginal/complicaciones , Linfoma de Células B de la Zona Marginal/diagnóstico , Linfoma de Células B de la Zona Marginal/tratamiento farmacológico , Fibrilación Atrial/complicacionesRESUMEN
OBJECTIVE: To compare the efficacy of laparoscopic pancreaticoduodenectomy (LPD) and open pancreaticoduodenectomy (OPD) in a medium-volume medical center. METHODS: Data for patients who underwent OPD or LPD for carcinoma of the ampulla of Vater (VPC) between January 2017 and June 2022 were acquired retrospectively. Propensity score-matching (PSM) analysis was performed to balance the baseline characteristics between the groups. The primary outcome was disease-free survival (DFS). Cox regression analysis was used to explore the independent risk factors for DFS. RESULTS: A total of 124 patients with pathologically diagnosed VPC were included. After 1:1 matching, there were 23 cases each in the OPD and LPD groups. Kaplan-Meier survival analyses showed that the median DFS in the OPD and LPD groups was identical (16.0 months vs 16.0 months, respectively). Multivariate Cox regression analysis showed that low levels of alkaline phosphatase and γ-glutamyl transpeptidase, positive surgical margin, and lymph node enlargement were independent risk factors for DFS. CONCLUSION: LPD in medium-volume centers with acceptable technical conditions may approach or even achieve the efficacy of LPD in large-volume centers.
Asunto(s)
Ampolla Hepatopancreática , Carcinoma , Laparoscopía , Neoplasias Pancreáticas , Humanos , Pancreaticoduodenectomía/efectos adversos , Ampolla Hepatopancreática/cirugía , Puntaje de Propensión , Estudios Retrospectivos , Complicaciones Posoperatorias/etiología , Neoplasias Pancreáticas/cirugía , Tiempo de InternaciónRESUMEN
The shuttle effect of lithium-sulfur (Li-S) batteries and the poor conductivity of sulfur (S) and lithium polysulfide severely limit their practical applications. Currently, compounding carbon materials with S is one of the effective ways to solve this problem. Therefore, green, low-cost chestnut inner shell biochar (CISC) with graded porous structure was used as the S carrier in this experiment, and carbon nanotubes (CNTs) coating was employed as the S protective layer to improve the electrical conductivity and inhibit the shuttle effect. The results showed that the CISC prepared in this experiment had a relatively high specific surface area (1135.11â m2 g-1 ), and the S loading rate was as high as 65.72 %. The graded porous structure and high specific surface area of CISC can increase the loading rate of S and thus increase the battery capacity. Meanwhile, the naturally contained O and N elements can improve the chemisorption of S. The initial discharge capacity of the CISC@S/CNTs battery at 0.1â C is 967.3â mAh g-1 , and the capacity retention rate is 74.3 % after 500 cycles. The unique composite structure improves the battery's electrical conductivity, reduces the dissolution of polysulfides, and enhances the battery cycle stability.
RESUMEN
Lanthanide luminescence nanothermometers (LNTs) provide microscopic, highly sensitive, and visualizable optical signals for reporting temperature information, which is particularly useful in biomedicine to achieve precise diagnosis and therapy. However, LNTs with efficient emissions at the long-wavelength region of the second and the third near-infrared (NIR-II/III) biological window, which is more favourable for in vivo thermometry, are still limited. Herein, we present a lanthanide-doped nanocomposite with Tm3+ and Nd3+ ions as emitters working beyond 1200 nm to construct a dual ratiometric LNT. The cross-relaxation processes among lanthanide ions are employed to establish a strategy to enhance the NIR emissions of Tm3+ for bioimaging-based temperature detection in vivo. The dual ratiometric probes included in the nanocomposite have potential in monitoring the temperature difference and heat transfer at the nanoscale, which would be useful in modulating the heating operation more precisely during thermal therapy and other biomedical applications. This work not only provides a powerful tool for temperature sensing in vivo but also proposes a method to build high-efficiency NIR-II/III lanthanide luminescent nanomaterials for broader bio-applications.
Asunto(s)
Elementos de la Serie de los Lantanoides , Nanocompuestos , Termometría , Luminiscencia , Temperatura , Termometría/métodosRESUMEN
Lanthanide-ion-doped upconversion materials have been widely used in biological detection, bioimaging, displays, and anticounterfeiting due to their abilities of real-time readings, high spatial resolution, and deep tissue penetration. The typically long fluorescence lifetimes of rare-earth nanoparticles, in the microsecond to millisecond range, make them useful in interference-free lifetime detection imaging. Most detection systems are accompanied by fluorescence resonance energy transfer (FRET), in which the lifetime of the luminescence center can be used as a signal to reveal the degree of FRET. Due to the complex energy level structure and complex energy transfer processes, the apparent lifetimes of upconversion nanoparticles (UCNPs) do not simply equal the decay time of the corresponding energy level, inducing an insignificant lifetime change in the upconversion detection system. In this study, the relationship between the apparent luminescence lifetime of upconversion and the decay rate of each energy level was studied by numerical simulations. It was proved that the apparent lifetime of the emission at 540 nm was mainly affected by the decay rate of Yb3+. We then constructed a nanocomposite with Rh1000 fluorophores loaded onto the surface of UCNPs to quench the sensitizer Yb3+. We found that the lifetime of the emission at 540 nm from Er3+ was affected to a large extent by the number of attached Rh1000 molecules, proving the greater influence on the apparent luminescent lifetime of Er3+ at 540 nm caused by quenching the Yb3+ excited state. The qualitative detection of hypochlorous acid (HClO) in vivo was also achieved using the luminescent lifetime as the signal.
Asunto(s)
Elementos de la Serie de los Lantanoides , Metales de Tierras Raras , Nanopartículas , Ácido Hipocloroso , Luminiscencia , Metales de Tierras Raras/química , Nanopartículas/químicaRESUMEN
Gold nanojets with various morphologies, from nanopillar to nanotip with up to 800â nm height, and finally to nanotip with droplet, are fabricated on gold thin film by a femtosecond laser irradiation. The near-field localized surface plasmon resonance (LSPR) and photothermal effects of gold nanojets are studied through finite element electromagnetic (EM) analysis, supporting in nanojets design for potential applications of high-resolution imaging, nanomanipulation and sensing. For an individual nanotip, the confined electron oscillations in LSPR lead to an intense local EM field up to three orders of magnitude stronger than the incident field strength at the end of gold tip, where the vertical resolution for the field enhancement was improved down to nanoscale due to the small size of the sharp gold tip (5-nm-radius). At specific wavelength, nanopillar can serve as an effective light-to-heat converter and its heating can be fine-tuned by external irradiation, and its dimension. The long-range periodic nanojet arrays (periods from 1.5 µm to 2.5 µm) with different geometry were printed using several pulse energy levels. By confining more light into the tip (two orders of magnitude stronger than single tip), nanotip array shows more pronounced potential to serve as a refractometric sensor due to their high sensitivity and reproducibility. These results promote fs laser printing as a high-precision tool for nanoarchitecture in optical imaging, nanomanipulation and sensing application.
RESUMEN
Multiple 11-fs infrared, few-cycle laser pulses were applied to a polycrystal ZnSe surface to study the evolution of surface damage morphologies. The polycrystalline grain boundaries seem to be the initiation site of surface damage and formation of ripples, which evolve as the result of many laser pulses at the same site. Scanning electron microscopy and atomic force microscopy (AFM) were applied to characterize the surface. The crystalline change and material phase transition were examined by confocal Raman spectroscopy. The thermal expansion coefficient increased slightly in the ablated zone compared to the non-ablated zone according to an AFM thermal tip test. The results show the growth and organization of surface ripples and the change of thermal properties as the number of irradiations at each site increases.
RESUMEN
To speed up the fabrication of optical metamaterials by making use of the fast speed advantage of femtosecond laser preparation, a metamaterial appropriate for femtosecond laser processing was designed, and the interaction between femtosecond laser and metal-dielectric-metal fishnet stacks was investigated in detail. Two kinds of processing mechanisms, thermal melting and stress break, were revealed during the fabrication. The thermal melting process, dominated by the interaction of femtosecond laser with metals, makes the upper and lower metal layers adhere to each other, which leads to the magnetic resonance impossible. The stress break process, dominated by the interaction of femtosecond laser with dielectrics, can keep the upper and lower metal coatings isolated. Fishnet optical metamaterial was fabricated by femtosecond laser-induced stress break technique, using back side ablation, high numerical aperture and super-Gaussian beam. The resolution and speed can reach 500 nm, and 100 units/s, respectively. Spectrophotometer measurement results proved that the magnetic resonances were found in the fishnet nanostructure. The theoretical refractive index of the metamaterial on a glass substrate reached -0.12 at the wavelength of 3225 nm. It proved that femtosecond laser-induced stress break was a good and fast tool during the fabrication of optical metamaterials.
RESUMEN
The interaction of high-intensity few-cycle laser pulses with solids opens a new area of fundamental light-material interaction research. The applied research extends from extreme nonlinearity in solids to the next-generation high laser light damage resistance optical design. In this Letter, 11 fs infrared, carrier-envelope-phase (CEP) stable, two-cycle laser pulses were applied to investigate the process of laser-material interaction on the ZnSe surface. A systematic study of a few-cycle pulse laser-induced damage threshold on ZnSe was performed for a single-pulse regime (1-on-1). Laser damage morphologies were carefully characterized. Our experiment demonstrated the very beginning of laser-induced structures on the ZnSe surface by using the shortest infrared few-cycle laser pulse currently available with a stable CEP.
RESUMEN
With ever-increasing laser power, the requirements for ultraviolet (UV) coatings increase continuously. The fundamental challenge for UV laser-resistant mirror coatings is to simultaneously exhibit a high reflectivity with a large bandwidth and high laser resistance. These characteristics are traditionally achieved by the deposition of laser-resistant layers on highly reflective layers. We propose a "reflectivity and laser resistance in one" design by using tunable nanolaminate layers that serve as an effective layer with a high refractive index and a large optical bandgap. An Al2O3-HfO2 nanolaminate-based mirror coating for UV laser applications is experimentally demonstrated using e-beam deposition. The bandwidth, over which the reflectance is >99.5%, is more than twice that of a traditional mirror with a comparable overall thickness. The laser-induced damage threshold is increased by a factor of ~1.3 for 7.6 ns pulses at a wavelength of 355 nm. This tunable, nanolaminate-based new design strategy paves the way toward a new generation of UV coatings for high-power laser applications.
RESUMEN
HfO2/SiO2 bilayer coatings and multilayer high-reflection coatings without and with a modified co-evaporated interface (MCEI) have been prepared. An MCEI is designed to be evaporated at an oxygen-deficient environment to achieve higher absorption than the conventional discrete interface. Capacitance-voltage measurements and absorption measurements demonstrate that an MCEI increases the trap density and leads to higher absorption. The laser-induced damage threshold and nano-indenter test results indicate that the MCEI multilayer coating exhibits better laser resistance and mechanical property, despite the larger absorption. The experimental results suggest that adhesive force between layers plays a more important role in nanosecond laser damage resistance than interface absorption.
RESUMEN
In the pursuit of 1064 nm high-power laser resistance dielectric coatings in the nanosecond region, a group of HfO2/SiO2 high reflectors with and without suture layers were prepared on prearranged fused silica substrates with femtosecond laser pits. Surface morphology, global coating stress, and high-resolution cross sections were characterized to determine the effects of substrate pit suturing. Laser-induced damage resistance was investigated for samples with and without suture layers. Our results indicate considerable stability in terms of the nanosecond 1064 nm laser-induced damage threshold for samples having a suture layer, due to decreased electronic field (e-field) deformation with simultaneous elimination of internal cracks. In addition, a suture layer formed by plasma ion-assisted deposition could effectively improve global mechanical stress of the coatings. By effectively reducing the multilayer deformation using a suture layer, electron-beam high-reflective coatings, whose laser-induced damage resistance was not influenced by the substrate pit, can be prepared.
RESUMEN
Nanosecond laser-resistance to dielectric multilayer coatings on substrate pits was examined with respect to the electric-field (E-field) enhancement and mechanical properties. The laser-induced damage sensitivity to the shape of the substrate pits has not been directly investigated through experiments, thus preventing clear understanding of the damage mechanism of substrate pits. We performed a systematic and comparative study to reveal the effects of the E-field distributions and localized stress concentration on the damage behaviour of coatings on substrates with pits. To obtain reliable results, substrate pits with different geometries were fabricated using a 520-nm femtosecond laser-processing platform. By using the finite element method, the E-field distribution and localized stress of the pitted region were well simulated. The 1064-nm damage morphologies of the coated pit were directly compared with simulated E-field intensity profiles and stress distributions. To enable further understanding, a simplified geometrical model was established, and the damage mechanism was introduced.
RESUMEN
Damage precursors in the 3ω (351 nm) mirror for a high-power laser system are investigated as well as the relevant damage mechanisms. The precursors are classified into two ensembles according to the different laser resistance and damage features. The former is nano-absorbing precursors, which are sensitive to the standing wave electric field and vulnerable to the laser irradiation. The latter is submicrometer nodular defects, which have higher laser resistance and are sensitive to the adhesion strength between the fluoride coatings and oxide coatings. The damage due to nano-absorbing precursors is efficiently suppressed with the double stack design that screens the electric field in the oxides. Currently, the nodular seed is major originating from the Al2O3/SiO2 stack. Even for the same defect type and mirror, the final damage features are dependent on the local mechanical properties at the irradiation location. The investigations of the damage mechanisms provide a direction to further improve the laser-induced damage threshold of the 3ω mirror.
RESUMEN
355 nm high-reflective multilayer coatings with or without coevaporated interfaces (CEIs) were prepared by electron beam evaporation under the same deposition condition. Their transmission spectra, surface roughness, and mechanical stress properties were evaluated. Elemental composition analysis of the multilayer interfaces was performed using x-ray photoelectron spectroscopy, and laser-induced damage thresholds were obtained in both 1-on-1 and 300-on-1 testing modes. The coatings with CEIs reveal a lower mechanical stress and a higher laser damage resistance when irradiated with high laser fluence, and the corresponding damage modeling indicates that CEIs can significantly decrease defect density. The resulting damage morphologies show that CEI coatings can significantly suppress coating delamination and exhibit a "bulk-like" damage behavior, demonstrating better damage performance against high-power lasers.
RESUMEN
The laser damage resistance of the coatings for high-power laser systems depends greatly on the surface quality of the substrate. In our work, experimental approaches were employed to understand the interface coupling effect of the substrate surface and coatings on the laser resistance of the coatings. A 1064 nm anti-reflection (AR) coating was deposited by an e-beam coater onto fused silica with and without micro-scale pits (structural defects). The micro-scale pits were precisely fabricated by femtosecond laser processing to prevent the emergence of subsurface cracks. Different deposition temperatures were characterized in order to verify the intensity of the interface coupling effect of the substrate and coating layers. Our experimental results indicate that impurities that are introduced in the finishing process, shifted to the substrate surface, and aggregated during the heating process, play a much more crucial role than structural defects (length: â¼7 µm; width: â¼3 µm; depth: â¼0.8 µm) in the laser-induced damage process. By effectively reducing the intensity of the interface coupling effect, the e-beam AR coatings, whose laser-induced damage resistance was closed to the bare substrate, was prepared.
RESUMEN
The near-field phase modulation (NFPM) caused by the plasma scalds is investigated with a newly proposed mixed overcoat layer model. Based on the NFPM, the far-field intensity modulation (FFIM) is calculated and discussed with the scalar diffraction theory. The results indicate that both the NFPM and FFIM are sensitive to the scalding depth. A feature curve is developed to analyze the NFPM for arbitrary scalding depth. The modulation can be ignored when the scalding depth is less than the first feature point in the feature curve. Even though the diffraction intensity in the Fresnel region can be enhanced dozens of times, the FFIM in the Fraunhofer region can recover gradually if the scalding depth is below a critical value. The preliminary experimental results are consistent with the theoretical prediction.
RESUMEN
A rigorous electromagnetic method is developed to analyze the resonance effect of near field caused by nanoscale subsurface defects, which play a key role in describing absorption enhancement during laser-matter interaction for transparent dielectric materials. The total electric field calculated with this new method is consistent with the result of finite-difference time-domain simulation. The concept of mode amplitude density spectrum is developed to analyze the specific modes of the total field. A new mode parameter is proposed to demarcate the contribution of the resonance. The frequency space is divided into four parts and the resonance effect is analyzed as well as the contributions of different modes to the total field. The influence of the structure parameters on the near-field modulation and energy transference is also discussed. It is found that the enhancement mechanism of the near-field and local absorption is the resonance effect caused by the total internal reflection on the sidewall of the nanostructure. In addition, the surrounding energy is mainly guided into the structure by the root of the structure via the energy flow analysis.