RESUMEN
We studied the effect of JNK and p53 inhibitors on the production of neurotrophic factors stimulating the realization of the growth potential of neural stem cells by neuroglial cells of various types under conditions of simulation of induced ß-amyloid neurodegeneration in vitro. It was shown that ß-amyloid stimulates the production of neurotrophins by astrocytes and microglial cells, but does not affect the functioning of oligodendrocytes. JNK and p53 were not involved in the secretion of neurotrophins by intact astrocytes. The stimulating role of p53 on the implementation of their secretory activity under the influence of a neurotoxic agent was revealed. At the same time, the inhibitory role of JNK and p53 in the production of neurotrophic growth factors by oligodendrocytes and microglial cells was revealed both under conditions of their optimal vital activity and when ß-amyloid was added to the cell culture.
Asunto(s)
Enfermedad de Alzheimer , Células-Madre Neurales , Humanos , Péptidos beta-Amiloides/metabolismo , Proteína p53 Supresora de Tumor/genética , Proteína p53 Supresora de Tumor/metabolismo , Astrocitos/metabolismo , Células-Madre Neurales/metabolismo , Factores de Crecimiento Nervioso/metabolismo , Enfermedad de Alzheimer/genética , Enfermedad de Alzheimer/metabolismoRESUMEN
Under conditions of neurodegeneration modeled in vitro by the ß-amyloid peptide-(25-35) fragment (Aß25-35), we studied the role of individual links of cAMP-dependent intracellular signaling pathways in determining the proliferation and differentiation status of neural stem cells (NSCs) and colony-stimulating activity of supernatants from neuroglial cells. The important role of intracellular cAMP and PKA in the inhibition of the progression of the NSC cell cycle and stimulation of the process of their specialization induced by Aß25-35 was found. The selective ability of PKA to block the production of factors constituting colony-stimulating activity by neuroglial cells under conditions of their cultivation in vitro with a neurotoxic agent was revealed. Our results suggests that inhibitors of adenylate cyclase and PKA can increase the degree of implementation of the growth potential of NSCs and conjugation of the processes of their proliferation and differentiation in Alzheimer's disease. At the same time, selective PKA blockers can also induce the production of NSC-stimulating factors by neuroglial cells.
RESUMEN
We studied the participation of JNK and p53 in the realization of the growth potential of different types of progenitors of the subventricular zone of mouse brain and secretion of neurotrophins by glial cells. The stimulating role of these signaling molecules in mitotic activity and specialization of multipotent neural stem cells was shown. It was found that JNK and p53 do not participate in the regulation of committed neuronal progenitor cells (clonogenic PSA-NCAM+ cells). A dependence of neurotrophic growth factors in individual populations of neuroglia on activity of these protein kinase and transcription factor was revealed. The role of JNK and p53 in astrocytes consists in stimulation of their secretion, and in microglial cells, on the contrary, in its inhibition. The secretory neurotrophic function of oligodendrogliocytes is not associated with JNK and p53 activity.
Asunto(s)
Astrocitos/metabolismo , MAP Quinasa Quinasa 4/genética , Células Madre Multipotentes/metabolismo , Células-Madre Neurales/metabolismo , Neuroglía/metabolismo , Proteína p53 Supresora de Tumor/genética , Animales , Astrocitos/citología , Astrocitos/efectos de los fármacos , Benzotiazoles/farmacología , Antígeno CD56/genética , Antígeno CD56/metabolismo , Medios de Cultivo Condicionados/farmacología , Regulación de la Expresión Génica , Ventrículos Laterales/citología , Ventrículos Laterales/efectos de los fármacos , Ventrículos Laterales/metabolismo , MAP Quinasa Quinasa 4/metabolismo , Ratones , Ratones Endogámicos C57BL , Células Madre Multipotentes/citología , Células Madre Multipotentes/efectos de los fármacos , Factores de Crecimiento Nervioso/biosíntesis , Factores de Crecimiento Nervioso/genética , Moléculas de Adhesión de Célula Nerviosa/genética , Moléculas de Adhesión de Célula Nerviosa/metabolismo , Células-Madre Neurales/citología , Células-Madre Neurales/efectos de los fármacos , Neuroglía/citología , Neuroglía/efectos de los fármacos , Transducción de Señal , Tolueno/análogos & derivados , Tolueno/farmacología , Proteína p53 Supresora de Tumor/metabolismoRESUMEN
We studied the effect of an anthocyanin-containing complex from Sorbus aucuparia L. on the main indicators of erythropoiesis in the blood and bone marrow of mice against the background of doxorubicin treatment. It was shown that administration of an anthocyanincontaining complex from S. aucuparia L. prevented the development of anemic syndrome by stimulating regeneration of the erythroid lineage of hematopoiesis after its devastation by single administration of the cytostatic.
Asunto(s)
Antocianinas/metabolismo , Eritropoyesis/fisiología , Anemia/genética , Anemia/metabolismo , Animales , Antocianinas/genética , Doxorrubicina/uso terapéutico , Eritropoyesis/genética , Hematopoyesis/genética , Hematopoyesis/fisiología , Masculino , Ratones Endogámicos C57BLRESUMEN
We studied the peculiarities of the participation of ERK1/2 and Ñ38 in regulation of various types of progenitor cells of the nervous tissue under conditions of ethanol-induced neurodegeneration modeled in vitro and in vivo. The stimulating role of these signaling molecules in the realization of the growth potential of intact multipotent neural stem cells and committed neuronal precursors (clonogenic PSA-NCAM+ cells) was demonstrated. In vitro exposure to neurotoxic doses of ethanol led to the loss of the specified role of ERK1/2 and p38 in the cell cycle regulation. Inversion of the role of both studied MAP-kinases in determining the proliferation status of neural stem cells after long-term administration of ethanol to experimental animals was revealed. In committed neuronal precursors, this inversion (inhibition of mitotic activity instead of activation) was revealed only for ERK1/2. In mice exposed to chronic alcoholization, ERK1/2 no longer participated in the process of specialization of both types of regeneration-competent cells of the nerve tissue. The revealed fundamental difference between the functions of ERK1/2 and p38 in the cell cycle regulation in neural stem cells and committed neuronal precursors under optimal conditions and during ethanol-induced neurodegeneration does not allow drawing definite conclusions about the prospect of using modifiers of their activity for the therapy for alcohol-related CNS pathologies.
Asunto(s)
Diferenciación Celular/efectos de los fármacos , Etanol/toxicidad , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Enfermedades Neurodegenerativas/metabolismo , Transducción de Señal/efectos de los fármacos , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismo , Animales , Ciclo Celular/efectos de los fármacos , Diferenciación Celular/genética , Flavonoides/farmacología , Imidazoles/farmacología , Ratones , Ratones Endogámicos C57BL , Células-Madre Neurales/efectos de los fármacos , Células-Madre Neurales/metabolismo , Enfermedades Neurodegenerativas/inducido químicamente , Piridinas/farmacología , Transducción de Señal/genética , Células Madre/efectos de los fármacos , Células Madre/metabolismoRESUMEN
Suppression of the production of granulocytic CSF under the effect of 5-fluorouracyl is related to disorders in the NF-κB-, cAMP-dependent signaling pathways and MAPK cascade. These secondary messengers are involved in the regulation of functional activity of nonadherent myelokaryocytes starting from day 10 of the experiment (initial period of the hemopoietic granulocytic stem regeneration after antimetabolite challenge). Granulocytic CSF does not play essential role in the formation of colony-stimulating activity of cells of the adherent and nonadherent fractions of the bone marrow. Only cAMP-dependent pathway is involved in the regulation of the realization of the granulocytic precursor growth potential in response to the challenge.
Asunto(s)
Citostáticos/farmacología , Fluorouracilo/farmacología , Factor Estimulante de Colonias de Granulocitos/genética , Granulocitos/efectos de los fármacos , Hematopoyesis/efectos de los fármacos , FN-kappa B/genética , Adenilil Ciclasas/genética , Adenilil Ciclasas/metabolismo , Animales , Médula Ósea/efectos de los fármacos , Médula Ósea/metabolismo , Adhesión Celular/efectos de los fármacos , AMP Cíclico/metabolismo , Didesoxiadenosina/análogos & derivados , Didesoxiadenosina/farmacología , Regulación de la Expresión Génica , Tiomalato Sódico de Oro/farmacología , Factor Estimulante de Colonias de Granulocitos/metabolismo , Granulocitos/citología , Granulocitos/metabolismo , Hematopoyesis/genética , Imidazoles/farmacología , Inyecciones Intraperitoneales , Masculino , Ratones , Ratones Endogámicos C57BL , FN-kappa B/antagonistas & inhibidores , FN-kappa B/metabolismo , Piridinas/farmacología , Transducción de Señal , Proteínas Quinasas p38 Activadas por Mitógenos/antagonistas & inhibidores , Proteínas Quinasas p38 Activadas por Mitógenos/genética , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismoRESUMEN
The role of NF-κB, cAMP/PKA, JAKs/STAT3, ERK1/2, p38, JNK, and p53 signaling pathways in the realization of growth potential of mesenchymal, neural, erythroid, and granulomonocytic progenitor cells were examined in vitro. Using selective blockers of signaling molecules, we revealed some principal distinctions of their involvement in determination of proliferation-differentiation status of the progenitor cells of different functional classes. The most salient peculiarities were observed in the roles of cAMP/PKA, JNK, and JAKs/STAT3 signaling pathways in the control of functions of various types of the regeneration-competent elements. The specific features of intracellular signaling revealed in histogenetically and functionally different progenitor cells attest to visibility of differentiated pharmacological stimulation of regeneration in individual tissues and prospectiveness in the development of targeted remedies for regenerative medicine based on modifiers of activity of the intracellular signaling molecules.
Asunto(s)
Células Madre Hematopoyéticas/metabolismo , Células Madre Mesenquimatosas/metabolismo , Células-Madre Neurales/metabolismo , Transducción de Señal/efectos de los fármacos , Animales , Antracenos/farmacología , Antraquinonas/farmacología , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Didesoxiadenosina/farmacología , Diterpenos de Tipo Kaurano/farmacología , Flavonoides/farmacología , Células Madre Hematopoyéticas/efectos de los fármacos , Quinasas Janus/metabolismo , Células Madre Mesenquimatosas/efectos de los fármacos , Ratones , Ratones Endogámicos C57BL , Proteínas Quinasas Activadas por Mitógenos , FN-kappa B/metabolismo , Células-Madre Neurales/efectos de los fármacos , Nitrilos , Fosforilación/efectos de los fármacos , Pirazoles/farmacología , Pirimidinas , Medicina Regenerativa , Factor de Transcripción STAT3/metabolismo , Sulfonamidas/farmacologíaRESUMEN
The in vitro and in vivo models of ethanol-induced neurodegeneration were used to evaluate the content and functional activity of various types of regeneration-competent cells in subventricular zone of the cerebral hemispheres in C57Bl/6JY mice. In nervous tissue culture, ethanol (65 mM) produced no effect on formation of neurospheres. When administered per os in a daily dose of 3 g/kg for 8 weeks, ethanol produced no effect on the number of neural CFU in situ. In both cases, ethanol reduced proliferative activity of neural CFU. Long-term administration of ethanol in vivo suppressed differentiation of neural stem cells and decreased the number of committed precursors (neural cluster-forming units) in the subventricular zone of cerebral hemispheres. In vitro application of ethanol stimulated secretion of humoral growth factors by the cluster-forming neural glial cells. In contrast, in vivo administration of ethanol suppressed this secretion.
Asunto(s)
Alcoholismo/patología , Cerebro/efectos de los fármacos , Etanol/farmacología , Ventrículos Laterales/efectos de los fármacos , Enfermedades Neurodegenerativas/patología , Neuronas/efectos de los fármacos , Alcoholismo/metabolismo , Animales , Recuento de Células , Diferenciación Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Cerebro/metabolismo , Cerebro/patología , Cerebro/fisiopatología , Modelos Animales de Enfermedad , Péptidos y Proteínas de Señalización Intercelular/agonistas , Péptidos y Proteínas de Señalización Intercelular/biosíntesis , Ventrículos Laterales/metabolismo , Ventrículos Laterales/patología , Ventrículos Laterales/fisiopatología , Ratones , Ratones Endogámicos C57BL , Células-Madre Neurales/efectos de los fármacos , Células-Madre Neurales/patología , Enfermedades Neurodegenerativas/metabolismo , Neuroglía/efectos de los fármacos , Neuroglía/metabolismo , Neuroglía/patología , Neuronas/patología , Cultivo Primario de Células , Esferoides Celulares/efectos de los fármacosRESUMEN
The role of JAK/STAT3-mediated signaling pathway in the realization of the growth potential of mesenchymal precursor cells was examined in vitro. The stimulating role of JAKs and STAT3 towards proliferating activity of progenitor cells and their different role in the regulation of differentiation of the progenitor elements were demonstrated. Inhibitors of JAKs and STAT3 reduced the yield of fibroblast CFU and their mitotic activity. Blockade of JAKs accelerated and selective inactivation of STAT3 decelerated differentiation of progenitor cells.
Asunto(s)
Células de la Médula Ósea/metabolismo , Fibroblastos/metabolismo , Janus Quinasa 1/genética , Janus Quinasa 3/genética , Células Madre Mesenquimatosas/metabolismo , Factor de Transcripción STAT3/genética , Animales , Antraquinonas/farmacología , Células de la Médula Ósea/citología , Células de la Médula Ósea/efectos de los fármacos , Diferenciación Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Regulación de la Expresión Génica , Janus Quinasa 1/antagonistas & inhibidores , Janus Quinasa 1/metabolismo , Janus Quinasa 3/antagonistas & inhibidores , Janus Quinasa 3/metabolismo , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/efectos de los fármacos , Ratones , Ratones Endogámicos C57BL , Nitrilos , Fosforilación , Cultivo Primario de Células , Pirazoles/farmacología , Pirimidinas , Factor de Transcripción STAT3/antagonistas & inhibidores , Factor de Transcripción STAT3/metabolismo , Transducción de Señal , Sulfonamidas/farmacologíaRESUMEN
We studied the role of some JAK in the effect of diterpene alkaloid songorine on realization of the growth potential of mesenchymal precursor cells. The participation of JAK1, JAK2, and JAK3 in stimulation of proliferation of the precursor cells was demonstrated. Specific inhibitors of these JAK reduced the yield of fibroblast CFU and the rate of their division. Inhibition of JAK2 against the background of songorine treatment increased the rate of precursor differentiation.
Asunto(s)
Alcaloides/farmacología , Janus Quinasa 1/metabolismo , Janus Quinasa 2/metabolismo , Janus Quinasa 3/metabolismo , Animales , Diferenciación Celular/efectos de los fármacos , Humanos , Janus Quinasa 1/genética , Janus Quinasa 2/genética , Janus Quinasa 3/genética , Células Madre Mesenquimatosas/efectos de los fármacos , Células Madre Mesenquimatosas/metabolismo , Inhibidores de Proteínas Quinasas/farmacología , Medicina Regenerativa/métodos , Transducción de Señal/fisiología , Células Madre/citología , Células Madre/metabolismoRESUMEN
The involvement of the studied signal cascades in the regulation of erythropoietin production by bone marrow nuclears under conditions of immobilization stress depends on the type of the hemopoiesis-inducing microenvironment cells and the period of blood system reaction to stress exposure. Secretory activity of monocytes is regulated mainly by PI3K improving cell resistance to disturbances. The functional role of signal cascades involved in the production of erythropoietin by T cells is determined by the stage of the common adaptation syndrome.
Asunto(s)
Adaptación Fisiológica , Eritropoyetina/genética , Hematopoyesis/genética , Leucocitos Mononucleares/metabolismo , Estrés Psicológico/genética , Animales , Células de la Médula Ósea/metabolismo , Células de la Médula Ósea/patología , Eritropoyetina/biosíntesis , Regulación de la Expresión Génica , Inmovilización , Leucocitos Mononucleares/patología , Masculino , Ratones , Ratones Endogámicos C57BL , Proteína Quinasa 1 Activada por Mitógenos/genética , Proteína Quinasa 1 Activada por Mitógenos/metabolismo , Proteína Quinasa 3 Activada por Mitógenos/genética , Proteína Quinasa 3 Activada por Mitógenos/metabolismo , FN-kappa B/genética , FN-kappa B/metabolismo , Fosfatidilinositol 3-Quinasas/genética , Fosfatidilinositol 3-Quinasas/metabolismo , Transducción de Señal , Estrés Psicológico/metabolismo , Estrés Psicológico/fisiopatología , Proteínas Quinasas p38 Activadas por Mitógenos/genética , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismoRESUMEN
Psychopharmacological effects of JNK inhibitor were studied using a mouse model of posthypoxic encephalopathy. The preparation exhibited a pronounced cerebroprotective effect manifested in normalization of orientation and exploratory behavior and conditioned responses in posthypoxic mice. These effects were accompanied by marked elevation of neural stem cell content in the paraventricular region of the brain.
Asunto(s)
Encefalopatías/tratamiento farmacológico , Proteínas Quinasas JNK Activadas por Mitógenos/antagonistas & inhibidores , Proteínas Quinasas JNK Activadas por Mitógenos/metabolismo , Animales , Antracenos/farmacología , Antracenos/uso terapéutico , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Encefalopatías/psicología , Conducta Exploratoria/efectos de los fármacos , Masculino , Ratones , Células-Madre Neurales/efectos de los fármacos , Medicina RegenerativaRESUMEN
The effects of Fructus Sorbi aucupariae extract, originally saturated with anthocyans, on the development of Lewis lung carcinoma and B-16 melanoma in C57Bl/6 mice and the efficiency of cyclophosphamide treatment were studied. Antitumor activity of the extract and potentiation of the antimetastatic activity of the cytostatic were demonstrated. Studies on melanoma B-16 model revealed an increase in the counts of stromal progenitor cells in the tumor node and their accelerated maturation after treatment with the extract. No effects towards the tumor stem and committed cells were detected.
Asunto(s)
Antocianinas/farmacología , Antineoplásicos Alquilantes/farmacología , Antineoplásicos Fitogénicos/farmacología , Carcinoma Pulmonar de Lewis/tratamiento farmacológico , Ciclofosfamida/farmacología , Melanoma Experimental/tratamiento farmacológico , Extractos Vegetales/farmacología , Animales , Antineoplásicos Fitogénicos/aislamiento & purificación , Carcinoma Pulmonar de Lewis/patología , Sinergismo Farmacológico , Quimioterapia Combinada , Femenino , Frutas/química , Inyecciones Intramusculares , Metástasis Linfática , Melanoma Experimental/patología , Ratones , Ratones Endogámicos C57BL , Trasplante de Neoplasias , Células Madre Neoplásicas/efectos de los fármacos , Células Madre Neoplásicas/patología , Extractos Vegetales/aislamiento & purificación , Sorbus/química , Carga Tumoral/efectos de los fármacos , Células Tumorales CultivadasRESUMEN
The leading role in the regulation of erythropoietic activity of adherent bone marrow cells under conditions of post-hemorrhagic anemia is played by classical MAP kinase pathway (ERK pathway). Erythropoietin is not the decisive factor in the formation of erythropoietic activity of adherent cells. PI3K, MAPK/ERK 1/2, and p38-signaling proteins are not the main regulators of local production of erythropoietin after 30% loss of circulating blood volume.
Asunto(s)
Anemia/genética , Eritropoyesis/genética , Hemorragia/genética , Proteína Quinasa 1 Activada por Mitógenos/genética , Proteína Quinasa 3 Activada por Mitógenos/genética , Fosfatidilinositol 3-Quinasas/genética , Proteínas Quinasas p38 Activadas por Mitógenos/genética , Anemia/metabolismo , Anemia/patología , Animales , Células de la Médula Ósea/efectos de los fármacos , Células de la Médula Ósea/metabolismo , Células de la Médula Ósea/patología , Cromonas/farmacología , Modelos Animales de Enfermedad , Eritropoyesis/efectos de los fármacos , Eritropoyetina/genética , Eritropoyetina/metabolismo , Flavonoides/farmacología , Regulación de la Expresión Génica , Hemorragia/metabolismo , Hemorragia/patología , Imidazoles/farmacología , Masculino , Ratones , Ratones Endogámicos C57BL , Proteína Quinasa 1 Activada por Mitógenos/antagonistas & inhibidores , Proteína Quinasa 1 Activada por Mitógenos/metabolismo , Proteína Quinasa 3 Activada por Mitógenos/antagonistas & inhibidores , Proteína Quinasa 3 Activada por Mitógenos/metabolismo , Morfolinas/farmacología , Fosfatidilinositol 3-Quinasas/metabolismo , Inhibidores de las Quinasa Fosfoinosítidos-3 , Inhibidores de Proteínas Quinasas/farmacología , Piridinas/farmacología , Transducción de Señal , Proteínas Quinasas p38 Activadas por Mitógenos/antagonistas & inhibidores , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismoRESUMEN
Mice with Lewis lung carcinoma were used to study the antitumor and antimetastatic effects of JAK3 inhibitor. The study revealed no effect of JAK3 inhibitor on the growth of primary tumor node, but found a pronounced inhibition of hematogenous spread of the pathologic process into the lungs. In vitro blockade of JAK3 in cultured Lewis lung carcinoma produced no effect on the count of the stem tumor cells and stimulated functions of committed elements. In addition, blockade of JAK3 significantly elevated maturation index of the tumor tissue.
Asunto(s)
Carcinoma Pulmonar de Lewis/tratamiento farmacológico , Carcinoma Pulmonar de Lewis/enzimología , Inhibidores Enzimáticos/uso terapéutico , Janus Quinasa 3/antagonistas & inhibidores , Animales , Inhibidores Enzimáticos/farmacología , Femenino , Ratones , Ratones Endogámicos C57BL , Células Madre Neoplásicas/efectos de los fármacos , Células Madre Neoplásicas/metabolismoRESUMEN
Involvement of individual JAK kinases in the realization of growth potential of mesenchymal progenitor cells was examined in vitro. Important role of JAK2 and JAK3 in determining the initial level of mitotic activity of progenitor cells was established. The yield of fibroblast CFUF was suppressed under the effect of specific inhibitors of JAK kinases. Blockade of JAK3 increased the rate of progenitor element differentiation. JAK1 had no effect on proliferation and differentiation status of progenitor cells.
Asunto(s)
Diferenciación Celular , Proliferación Celular , Quinasas Janus/fisiología , Células Madre Mesenquimatosas/enzimología , Animales , Células Cultivadas , Sistema de Señalización de MAP Quinasas , Masculino , Ratones Endogámicos CBARESUMEN
Psychopharmacological effects of atisine-type diterpene alkaloid Z77 were studied under conditions of experimental posthypoxic encephalopathy. The preparation had a pronounced cerebroprotective effect consisting in normalization of orientation and exploratory behavior and conditioned activity in experimental animals. These changes were accompanied by significant increase in the number of neural stem cells in the paraventricular region of the brain and markedly enhanced production of neurotrophic growth factors by neural tissue microenvironment cells.
Asunto(s)
Alcaloides/farmacología , Alcaloides/uso terapéutico , Encefalopatías/tratamiento farmacológico , Animales , Encéfalo/efectos de los fármacos , Conducta Exploratoria/efectos de los fármacos , Masculino , Ratones , Células-Madre Neurales/efectos de los fármacos , Medicina RegenerativaRESUMEN
We studied the mechanisms of erythropoiesis stimulation and effectiveness of Poetam, a preparation containing release-active anti-erythropoietin antibodies as a supplement treatment for experimental iron deficiency anemia during gestation. The results confirmed potency of combined therapy to stimulate erythropoiesis, which was more efficacious in comparison with monotherapy as assessed by the count of erythrokaryocytes and erythroid progenitors in the hematopoietic tissue as well as by the content of erythrocytes and hemoglobin in the peripheral blood. Activation of erythropoiesis is related to the modulatory effect of Poetam on proliferative activity and differentiation of erythroid precursors, which in most aspects results from stimulatory action of Poetam on secretion of the hematopoietically active factors by adherent elements of the hematopoietic microenvironment.
Asunto(s)
Anemia Ferropénica/tratamiento farmacológico , Anticuerpos/farmacología , Complicaciones del Embarazo/tratamiento farmacológico , Anemia Ferropénica/sangre , Animales , Anticuerpos/uso terapéutico , Evaluación Preclínica de Medicamentos , Recuento de Eritrocitos , Células Eritroides/efectos de los fármacos , Eritropoyesis/efectos de los fármacos , Femenino , Ratones Endogámicos C57BL , Embarazo , Complicaciones del Embarazo/sangreRESUMEN
We studied the dynamics of erythropoiesis in CBA mice during gestation against the background of treatment with iron-binding drug. The mechanisms of suppression of the bone marrow erythroid stem were evaluated. Administration of deferoxamine in a dose of 1 g/kg induced hypoplasia of the erythroid hemopoietic lineage. Suppression of bone marrow erythropoiesis manifested in a decrease of hemoglobin concentration and counts of reticulocytes, erythrocytes, and erythrokaryocytes. These changes were accompanied by a decrease in functional activity of erythropoietic precursors and secretion of erythropoietically active humoral factors by bone marrow myelokaryocytes. These data indicate that deferoxamine can be used for modeling of iron defi ciency anemia in pregnancy.
Asunto(s)
Anemia Ferropénica/inducido químicamente , Médula Ósea/efectos de los fármacos , Deferoxamina/farmacología , Eritropoyesis/efectos de los fármacos , Eritropoyesis/fisiología , Sideróforos/farmacología , Animales , Recuento de Células Sanguíneas , Médula Ósea/metabolismo , Eritrocitos/citología , Femenino , Ratones , Ratones Endogámicos CBA , Embarazo , Reticulocitos/citologíaRESUMEN
PI3- and MAP-kinase signaling pathways duplicate and interchange each other in production of agents that determine total erythropoietic activity under conditions of balanced erythropoiesis. The alternative p38-dependent MAP-kinase pathway is the major regulator of erythropoietic activity of adherent bone marrow cells. Blockade of PI3K and p38 signaling pathways stimulated production of erythropoietin by cells that do not produce it constitutively.