Asunto(s)
Enfermedades de los Peces/virología , Modelos Moleculares , Nodaviridae/efectos de los fármacos , Infecciones por Virus ARN/virología , ARN Polimerasa Dependiente del ARN/metabolismo , Ribavirina/farmacología , Replicación Viral/efectos de los fármacos , Secuencia de Aminoácidos , Animales , Lubina/virología , Línea Celular , Nodaviridae/enzimología , Nodaviridae/fisiología , Estructura Terciaria de Proteína , Alineación de SecuenciaRESUMEN
Hereditary haemorrhagic telangiectasia, HHT, is an autosomal dominant disorder that affects approximately 1 in 8000 people. HHT1 is associated with mutations in the ENG (Endoglin) gene and with haploinsufficiency. The disorder is characterized by focally dilated vessels, which can lead to arteriovenous malformations and serious complications even in young children. In the current study, umbilical cord and placenta samples from newborns with ENG mutations were analyzed to estimate the level of corresponding protein and look for potential vascular dysplasia. We confirmed, using metabolic labelling and flow cytometry, that endoglin levels were significantly reduced to median values of 47 per cent (range 32-56 per cent) and 58 per cent (46-90 per cent), respectively, in human umbilical vein endothelial cells derived from newborns with ENG mutations (HHT1 group; n=18) relative to samples from newborns shown not to have the familial mutation (non-HHT group). We also quantified the relative expression of endoglin by estimating the endoglin/PECAM-1 staining ratio in tissue sections. We observed significantly lower values in the HHT1 group, compared to the non-HHT group for the umbilical vein (n=9; median 0.6 vs 0.9; ranges 0.2-1.0 and 0.5-1.5) and for placental stem villus vessels (n=9 and 10; median 0.42 vs 0.93; ranges 0.24-0.58 and 0.56-1.18). No differences in the estimated umbilical vein cross-sectional area and in the proportion of vessels present in placental villi were observed in sections from the HHT1 group relative to the non-HHT group. Thus, blood vessels from HHT1 individuals are maintained intact in the umbilical vein and placenta during pregnancy and delivery, despite a significant reduction in endoglin expression.
Asunto(s)
Endotelio Vascular/metabolismo , Hiperbilirrubinemia Hereditaria/metabolismo , Placenta/irrigación sanguínea , Venas Umbilicales/metabolismo , Molécula 1 de Adhesión Celular Vascular/metabolismo , Antígenos CD , Células Cultivadas , Análisis Mutacional de ADN , Endoglina , Endotelio Vascular/citología , Humanos , Hiperbilirrubinemia Hereditaria/genética , Hiperbilirrubinemia Hereditaria/patología , Procesamiento de Imagen Asistido por Computador , Recién Nacido , Mutación , Molécula-1 de Adhesión Celular Endotelial de Plaqueta/metabolismo , Receptores de Superficie Celular , Venas Umbilicales/citología , Molécula 1 de Adhesión Celular Vascular/genéticaAsunto(s)
Ligasas/química , Ligasas/metabolismo , Transactivadores/química , Transactivadores/metabolismo , Ubiquitinas/metabolismo , Secuencias de Aminoácidos , Sitios de Unión , Cristalografía por Rayos X , Humanos , Modelos Moleculares , Unión Proteica , Conformación Proteica , Procesamiento Proteico-Postraduccional , Relación Estructura-Actividad , Especificidad por Sustrato , Enzimas Ubiquitina-Conjugadoras , Ubiquitinas/biosíntesisRESUMEN
Bis(maleidophenyl)-PEG2000 (Bis-Mal-PEG2000), a new bifunctional protein cross-linker targeted to sulfhydryl groups, introduces intra-tetrameric cross-links into oxy-HbA in nearly quantitative yields. Structural as well as crystallographic analyses of the cross-linked species, Bis-Mal-PEG2000 HbA, identified Cys-93(beta) as the site of intramolecular cross-linking. The cross-bridging had only a limited influence on the O(2) affinity and cooperativity of HbA in 50 mM BisTris acetate, pH 7.4. However, the Bohr effect was reduced by approximately 60%. Bis-Mal-PEG2000 HbA retained sensitivity to the presence of allosteric effectors 2, 3-diphosphoglycerate, IHP, and chloride, albeit to a lesser degree compared with HbA. Crystallographic analysis revealed the overall structure of deoxy-Bis-Mal-PEG2000 HbA to be similar to deoxy-HbA but for the loss of the salt bridge between Asp-94(beta) and His-146(beta). The large influence of the cross-bridging on the alkaline Bohr effect of HbA is consistent with the loss of this salt bridge. Unlike the "central cavity cross-bridges" described previously, the cross-link introduced by Bis-Mal-PEG2000 into HbA is an "outside the central cavity cross-bridge." In view of its oxy-conformational specificity and limited influence on O(2) affinity, this new cross-linking strategy holds promise for the stabilization of new designer low O(2) affinity Hbs generated by recombinant DNA technology for applications as Hb based therapeutics.
Asunto(s)
Reactivos de Enlaces Cruzados/farmacología , Cisteína/metabolismo , Hemoglobina A/química , Maleimidas/síntesis química , Maleimidas/farmacología , Polietilenglicoles/síntesis química , Polietilenglicoles/farmacología , Tampones (Química) , Cromatografía en Gel , Cromatografía Líquida de Alta Presión , Cristalografía por Rayos X , Electroforesis en Gel de Poliacrilamida , Humanos , Concentración de Iones de Hidrógeno , Maleimidas/aislamiento & purificación , Modelos Moleculares , Oxígeno/metabolismo , Polietilenglicoles/aislamiento & purificación , Unión Proteica , Compuestos de Sulfhidrilo/metabolismo , Factores de TiempoRESUMEN
Although numerous reports have documented that the S-nitrosylation of cysteine residues by NO alters the activities of a wide variety of proteins, the direct visualization and the structural consequences of this reversible modification have not yet been reported for any protein. Here we describe the crystal structure of S-nitroso-nitrosylhemoglobin determined at a resolution of 1.8 A. The specific reaction of NO with Cys93beta is confirmed in this structure, and a large S-nitrosylation-induced change in the tertiary structure of the COOH-terminal dipeptides of the beta subunits provides additional insight into the stereochemical mechanism by which blood flow is regulated by the interaction of NO with hemoglobin.