RESUMEN
Viral infections, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are some of the most dangerous threats to humans. SARS-CoV-2 has caused a global pandemic, highlighting the unprecedented demand for rapid and portable diagnostic methods. To meet these requirements, we designed a label-free colorimetric platform that combines the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated proteins (Cas) 12a system for naked-eye detection (named LFP). This method utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP) and the trans-cleavage activity of the CRISPR/Cas12a system to increase the sensitivity and specificity of the reaction. This platform can detect as few as 4 copies/µL of RNA and produces no false positive results when tested against the influenza virus. To better meet the requirements of point-of-care (POC) detection, we developed a portable device that can be applied in resource-poor and densely populated regions. The LFP assay holds great potential for application in resource-limited settings, and the label-free gold nanoparticle (AuNPs) probe can reduce costs, making it suitable for large-scale screening. We expect that the LFP assay will be promising for the POC screening of COVID-19.
Asunto(s)
Colorimetría , Oro , Nanopartículas del Metal , Técnicas de Amplificación de Ácido Nucleico , ARN Viral , SARS-CoV-2 , Oro/química , Colorimetría/métodos , Colorimetría/instrumentación , Nanopartículas del Metal/química , SARS-CoV-2/genética , SARS-CoV-2/aislamiento & purificación , ARN Viral/análisis , ARN Viral/genética , Técnicas de Amplificación de Ácido Nucleico/métodos , Técnicas de Amplificación de Ácido Nucleico/instrumentación , Humanos , COVID-19/diagnóstico , COVID-19/virología , Sistemas CRISPR-Cas , Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas , Técnicas de Diagnóstico MolecularRESUMEN
We developed an asymmetric decarboxylative allylic alkylation of vinylethylene carbonates with α-fluoro pyridinyl acetates through a synergistic palladium/copper catalysis. This protocol provides chiral allylic alcohol with carbon-fluorine quaternary stereogenic centers in good yield with good enantioselectivities and excellent regioselectivities. The utility of this approach was further demonstrated via a gram-scale experiment and derivatizations of the product.
RESUMEN
A green photo-oxidation reaction was discovered in which the imidazole ring is oxidized and rearranged to generate imidazolinone derivatives with thermal activation delayed fluorescence properties. The excellent photo-oxidation properties of p-PTZ-PIM can be utilized for detecting trace oxygen in the water phase and the sealing of food packaging bags.
