Immunoexpression of N-cadherin, Twist and Vimentin in Bladder Urothelial Carcinomas.

PURPOSE: Epithelial mesenchymal transition consists in the acquisition of neoplastic epithelial cells of a mesenchymal phenotype the process being involved in cancers invasion and metastasis. In this study were analyzed the expression of N-cadherin, Twist and Vimentin in bladder urothelial carcinomas according to the main prognostic parameters. MATERIAL/METHODS: The study included 20 bladder urothelial carcinomas which were analyzed histopathological, immunohistochemical and statistical. RESULTS: N-cadherin was identified in 45% of cases, which belonged to high-grade carcinomas with deep invasion and lymph node metastases. Twist immunoreaction was identified in all cases and was significantly increased in advanced stages carcinomas. Vimentin was present only in the advancing edge in 25% of cases, which belonged to highly invasive carcinomas. Urothelial carcinoma metastases were N-cadherin and Twist and Vimentin negative. We found a linear positive distribution of N-cadherin and Twist values. CONCLUSION: the used markers are useful for identifying aggressive urothelial carcinomas in the context of reciprocal stimulation mechanisms inside of urothelial epithelial-mesenchymal transition process.

[Clinical and therapeutical considerations regarding the diagnosis and treatment in meconial disease]. / Consideratii clinico-terapeutice privind diagnosticul si tratamentul bolii meconiale.

The meconium disease represents a problem of great impact over the vital prognosis of the newborn, the mortality of this disease being rated in various studies between 30 and 50%. The meconial disease that reunites the meconial ileus and the meconial peritonitis is often associated with cystic fibrosis a disease of general impact over the entire organism increasing the gravity and influencing the vital prognosis of these patients. The authors' purpose is to evaluate the results obtained after the treatment of the meconial disease in the Pediatric Surgery Clinic of the Emergency Hospital of Craiova over a period of 10 years. The study has been made on a number of 16 cases of meconial ileus, plug and peritonitis analising the difficulty of the diagnostic process, the preoperative care as well as the possibilities of surgical treatment. The surgical treatment is composed of multiple procedures including simple suture of the bowel defect and segmentary bowel resections with primary anastomosis and/or secondary anastomosis after ileostoma. The mortality on the studied cases was of 37.5% despite recent progresses achieved in postoperative care of these type of patients.

[Clinical and therapeutical considerations regarding a rare case of pre-pyloric stenosis]. / Consideratii clinico-terapeutice privind un caz rar de stenoza prepilorica.

The neonatal obstacle caused by a pre-pyloric diaphragm represent a rare cause of high oclusion in new-born. We present the case of a 6 days old new-born admitted in our department for nonbilious vomiting and feeding intolerance in which the clinical exam and the imagistic explorations (plain and contrast abdominal X-Rays, and ultrasound) could not reveal an evident cause for the oclusion. The surgical intervention imposed by the simptoms revealed the existece of a complete diaphragm in the prepyloric region. The initial excision of the diaphragm was not sufficient, the patient undergoing a second surgical intervention of gastrojejunal anastromosis with favorable evolution this time. The authors are presenting diagnosis and theraputical management peculiarities of this rare condition.

[Evolutive particularities of appendicular plastron in children]. / Particularitati evolutive ale blocului apendicular la copil.

This paper analyses the experience of the Paediatric Surgery Department from the Emergency Hospital in Craiova regarding the clinical and therapeutical evaluation of 55 cases with appendicular plastron admitted in our department between 1997-2006. We analyse both the evolution and the complications in managing these cases, together with particular aspects of differential diagnosis related to this group of age. These 55 cases were children aged between 2 and 15 years with a 15 days average hospitalization period. Applying a standard treatment we had favorable results in 85% of cases with 15% cases underwent surgical treatment from the first admission. Comparative to a study realised in our dept. between 1975-1996, which registered 30 cases with a mortality of 6.70%, the present one revealed in the latest years an important increase of the number of appendicular plastron with a significant low mortality.