RESUMEN
Failed reperfusion after thrombolytic therapy for acute myocardial infarction is common and signifies a poor prognosis. We investigated the clinical consequences of non-resolution of the ST segment after thrombolytic therapy for acute ST-elevation myocardial infarction, in 85 consecutive patients admitted to a coronary care unit lacking rapid access to angioplasty. Failed thrombolysis was defined as <50% ST-segment resolution 180 minutes after the start of thrombolytic treatment. Outcomes were measured in terms of in-hospital adverse events, length of hospital stay, and mortality at 6 weeks and 1 year. Thrombolysis was successful, in terms of ST-segment resolution, in 45 patients (53%). After adjustment for other factors, ST resolution was the only independent predictor of an uncomplicated recovery in hospital (odds ratio 6.8, 95% confidence interval 2.3 to 19.9; P<0.001). At 6 weeks and 1 year, overall mortality was lower in the ST resolution group, though these differences became non-significant on multivariate analysis. In patients who survived to hospital discharge, median length of stay was greater in successfully thrombolysed patients (9 days versus 8 days) despite their lower rate of complications. ST-segment resolution is a useful marker of successful thrombolysis and relates to clinical outcome. If assessed routinely it might assist, along with other clinical markers, in the identification of low-risk patients who can be discharged early.
Asunto(s)
Infarto del Miocardio/tratamiento farmacológico , Terapia Trombolítica/métodos , Anciano , Estudios de Cohortes , Recolección de Datos , Electrocardiografía , Femenino , Fibrinolíticos/uso terapéutico , Humanos , Tiempo de Internación , Masculino , Persona de Mediana Edad , Estreptoquinasa/uso terapéutico , Activador de Tejido Plasminógeno/uso terapéutico , Resultado del TratamientoRESUMEN
Transforming growth factor beta (TGFbeta) is thought to play an important role in the development and/or progression of a number of vascular disorders through its numerous effects on vascular smooth muscle cells (VSMCs). In this study we sought to identify and characterize TGFbeta-regulated VSMC genes using differential mRNA display (DD-RT-PCR) analysis of RNA isolated from TGFbeta-stimulated cultured rat aortic VSMCs. Northern blot analysis was used to demonstrate that five of 19 differentially displayed bands identified represented VSMC transcripts differentially expressed by TGFbeta. DNA sequencing revealed that three of these TGFbeta regulated genes were novel whilst the remaining two were identified through homologies to known genes. One TGFbeta upregulated transcript represented the protease cathepsin B. Since cathepsins may play a role in TGFbeta activation, an enzyme-linked immunosorbent assay (ELISA) for active TGFbeta1 was used to demonstrate an effect of cathepsin B on TGFbeta1 activation in vitro using both recombinant and human serum platelet-derived latent TGFbeta1 as substrate. These results suggest that induction of cathepsin B by TGFbeta, and its ability to activate TGFbeta1, may represent a mechanism whereby the autocrine action of TGFbeta is facilitated through expression of a protein which can process its latent form.
Asunto(s)
Regulación de la Expresión Génica , Factor de Crecimiento Transformador beta/metabolismo , Animales , Northern Blotting , Catepsina B/genética , Catepsina B/metabolismo , Regulación de la Expresión Génica/efectos de los fármacos , Humanos , Músculo Liso Vascular/citología , ARN Mensajero , Ratas , Ratas Wistar , Análisis de Secuencia de ADN , Factor de Crecimiento Transformador beta/genética , Factor de Crecimiento Transformador beta/farmacologíaRESUMEN
Endoglin, the gene linked to the autosomal dominant vascular disorder hereditary hemorrhagic telangiectasia type 1 (HHT1), encodes a 95-kDa membrane-bound proteoglycan which binds TGF beta 1 and regulates signaling via the type I and II TGF beta receptors on the surface of vascular endothelial cells. Using reverse-transcription polymerase chain reaction (RT-PCR) and Northern blot analysis we have shown that endoglin mRNA is expressed in both cultured human VSMCs and VSMCs freshly isolated from human aortas. Northern blot analysis was also used to demonstrate that endoglin expression decreased in serum-stimulated cultured human VSMCs but could be maintained by exogenous TGF beta 1. Endoglin protein expression in human VSMCs was shown by immunocytochemistry. These data, the first describing the existence of endoglin in VSMCs, suggest that through regulating TGF beta 1 signaling endoglin may mediate the effects of TGF beta 1 on VSMC behavior in vitro and in vivo.
Asunto(s)
Músculo Liso Vascular/metabolismo , ARN Mensajero/biosíntesis , Molécula 1 de Adhesión Celular Vascular/biosíntesis , Molécula 1 de Adhesión Celular Vascular/genética , Antígenos CD , Aorta Torácica , Secuencia de Bases , Células Cultivadas , Endoglina , Femenino , Humanos , Datos de Secuencia Molecular , Músculo Liso Vascular/química , Músculo Liso Vascular/efectos de los fármacos , Proteoglicanos/biosíntesis , Proteoglicanos/genética , ARN Mensajero/efectos de los fármacos , ARN Mensajero/aislamiento & purificación , Receptores de Superficie Celular , Factor de Crecimiento Transformador beta/farmacología , Molécula 1 de Adhesión Celular Vascular/efectos de los fármacos , Molécula 1 de Adhesión Celular Vascular/aislamiento & purificaciónRESUMEN
Replication-deficient adenoviral vectors have been widely used for gene transfer with the aim of delivering genes of interest to investigate their function and potentially to treat human disease. The ability to critically evaluate the biological role of a gene of interest, using adenovirus-based vectors, has been hampered by the development of local inflammation at the site of delivery. We have demonstrated that high multiplicity infection of human VSMCs with a replication-deficient adenoviral vector expressing no transgene leads to activation of the transcription factor NF kappa B. Activation of NF kappa B by this mechanism was able to augment gene expression from the human cytomegalo-virus immediate-early promoter (CMV-IEP) and induce expression of the adhesion molecule ICAM-1 in human VSMCs. These effects were inhibited by pretreatment with N alpha-p-tosyl1-L-lysine chloromethyl ketone (TLCK), a serine protease inhibitor known to inhibit the activation of NF kappa B. This important effect of the vector itself may have profound implications when replication-deficient adenoviral vectors are used for experimental gene transfer at a high multiplicity of infection.
Asunto(s)
Adenoviridae , Regulación de la Expresión Génica , Técnicas de Transferencia de Gen , Músculo Liso Vascular/metabolismo , FN-kappa B/genética , Células Cultivadas , Citomegalovirus/genética , Genes Inmediatos-Precoces , Vectores Genéticos , Humanos , Molécula 1 de Adhesión Intercelular/genética , Inhibidores de Serina Proteinasa/farmacología , Clorometilcetona Tosilisina/farmacología , Activación Transcripcional , Carga Viral , Replicación ViralRESUMEN
The severity of cardiac infiltration in AL amyloidosis is unrelated to whole body amyloid load as measured by serum amyloid P (SAP) tracer studies. Radiolabeled SAP and echocardiography permit identification of patients with severe cardiac disease with a low whole body load who may be the best candidates for transplantation.
Asunto(s)
Amiloide/metabolismo , Amiloidosis/fisiopatología , Cardiomiopatías/fisiopatología , Ecocardiografía Doppler , Componente Amiloide P Sérico/farmacocinética , Adulto , Anciano , Cardiomiopatías/patología , Femenino , Ventrículos Cardíacos/patología , Humanos , Radioisótopos de Yodo , Masculino , Persona de Mediana Edad , Factores de Tiempo , Función Ventricular Izquierda/efectos de los fármacosRESUMEN
The underlying cause of heart failure should be established, where possible. In particular the failure to respond to diuretic and vasodilator therapy requires careful evaluation.
Asunto(s)
Amiloidosis/complicaciones , Captopril/uso terapéutico , Cardiomiopatías/complicaciones , Anciano , Cardiomiopatías/tratamiento farmacológico , Diuréticos/uso terapéutico , Resultado Fatal , Insuficiencia Cardíaca/tratamiento farmacológico , Insuficiencia Cardíaca/etiología , Humanos , MasculinoRESUMEN
Recombinant adenoviral vectors are being used increasingly for gene transfer studies in mammalian cells and gene therapy protocols in humans. High adenoviral titers are often required for successful transduction of vascular smooth muscle cells (VSMCs), defined as uptake and detectable expression of the foreign gene, but the relative contributions of efficiency of viral uptake and control of transcription are poorly understood. To explore the extent to which a lack of detectable gene expression may be due to inefficient transcription of a successfully transferred gene, we have used a replication-deficient adenovirus expressing beta-galactosidase (RAd35 beta-Gal), under the control of the human cytomegalovirus major immediate-early promoter (CMV-IEP), which contains cAMP and nuclear factor-kappa B response elements, to investigate constitutive and inducible gene expression after gene transfer into human VSMCs. Histochemical staining with 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-gal), a quantitative spectrophotometric assay, SDS-PAGE, Western blotting, and Northern analysis were used to evaluate beta-galactosidase expression in infected cells. After infection with RAd35 beta-Gal at 30, 100, and 1000 plaque-forming units per cell (pfu/cell), expression of beta-galactosidase was augmented up to 17-, 19-, and 23-fold, respectively, in human VSMCs treated with forskolin and phorbol ester compared with unstimulated cells. After infection, the proportion of detectably transduced cells was increased by enhancer stimulation from 58% to 100% at 100 pfu/cell and from 9% to 62% at 10 pfu/cell, indicating quiescent viral DNA in unstimulated cells. At high adenoviral titers (1000 pfu/cell), the recombinant gene became the most abundant protein in cell extracts. These findings demonstrate that in human VSMCs, limited constitutive expression from the CMV-IEP, rather than failure of translocation of adenoviral DNA, may be responsible for the apparent failure of transduction at a low multiplicity of infection.
Asunto(s)
Adenoviridae , Expresión Génica , Técnicas de Transferencia de Gen , Vectores Genéticos , Músculo Liso Vascular/fisiología , Células Cultivadas , Humanos , beta-Galactosidasa/genéticaAsunto(s)
Competencia Clínica , Evaluación Educacional/métodos , Humanos , Examen Físico , Sociedades MédicasAsunto(s)
Complicaciones del Trabajo de Parto/inducido químicamente , Trabajo de Parto Prematuro/tratamiento farmacológico , Edema Pulmonar/inducido químicamente , Ritodrina/efectos adversos , Adulto , Femenino , Humanos , Embarazo , Embarazo Múltiple , Gemelos , Contracción Uterina/efectos de los fármacosRESUMEN
We report the case of an adult West Indian patient who presented with heart failure 20 years after an initial diagnosis of pulmonary sarcoidosis. Endomyocardial biopsy revealed AL type amyloid which was later found to be secondary to an underlying multiple myeloma.