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OBJECTIVE: Efforts have been made to better risk stratify patients given the rise in incidence of endometrial cancer (EC). The 2023 FIGO staging now incorporates histologic subtype and molecular classification into determination of EC stage. We sought to elucidate if the new staging system demonstrated prognostic differences compared to the 2009 staging system. METHODS: A retrospective chart review was performed on women treated for EC at our institution from September 2013 to May 2023 and combined with the publicly available TCGA Nature 2013 dataset. Detailed clinical information was captured. Patients were restaged according to the 2023 guidelines. Survival estimates were obtained using Kaplan-Meier method, and the log-rank test was used to compare survival curves for progression-free survival (PFS). RESULTS: 919 patients were included in our analysis. The datasets were comparable regarding histologic grade, stage, and age at diagnosis. 175 (31.5%) of patients in the institution dataset and 115 (31.6%) patients in the TCGA dataset experienced a stage change. Most patients whose stage changed were upstaged (275/290; 94.8%). 3-year PFS estimates for stage IA patients with no stage change versus those upstaged were 92.3% (95% CI: 87.2, 95.4) v. 72.0% (95% CI: 68.4, 84.9), p = 0.002. No significant differences in survival difference were seen in other stage subsets. CONCLUSION: Modest survival differences exist in patients with EC originally staged as IA who underwent upstaging. No significant survival difference is observed in patients who are restaged to stage II or III subsets. Improved risk stratification is needed in assessing prognosis and adjuvant therapy for patients with endometrial cancer.
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OBJECTIVES: Since the Landmark Shelby V. Holder Supreme Court Ruling, the number of laws in the United States that make it difficult to vote has increased dramatically. This may lead to legislation that limits access to health care, including options for family planning services. We determine whether voting restrictions are associated with county-level teenage birth rates. STUDY DESIGN: This is an ecological study. METHODS: The Cost of Voting Index, a state-level measure of barriers to voting during US elections from 1996 to 2016, was used as a proxy for access to voting. County-level teenage birth rates were obtained from the County Health Rankings data. We used multilevel modeling to determine whether restrictive voting laws were associated with county-level teenage birth rates. We tested whether associations varied across racial and socio-economic groups. RESULTS: When confounders were included, a significant association was observed between increasing voting restrictions and teenage birth rates (ß = 1.72, 95% confidence interval: 0.54, 2.89). A Cost of Voting Index-median income interaction term was tested and was statistically significant (ß = -1.00, 95% confidence interval: -1.36, -0.64), indicating that the observed relationship was particularly strong among lower-income counties. The number of reproductive health clinics per capita within each state is a potential mediator. CONCLUSION: Restrictive voting laws were associated with higher teenage birth rates, particularly for low-income counties. Future work should use methods in which a causal relation can be identified.
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Tasa de Natalidad , Renta , Adolescente , Humanos , Estados Unidos/epidemiología , Servicios de Planificación Familiar , Inequidades en Salud , PolíticaRESUMEN
Studies were conducted in 2020 and 2021 at the Delta Research and Extension Center in Stoneville, MS to determine concentrations of chlorantraniliprole (Prevathon, FMC Corporation, Philadelphia, PA) in soybean (Glycine max L.) leaves and florets. Chlorantraniliprole was applied as a foliar spray at four rates (0.028, 0.053, 0.078, 0.103 kg ai ha-1) for leaves and two rates (0.053, 0.078 kg ai ha-1) for florets. Leaf bioassays with corn earworm, Helicoverpa zea (Boddie), were conducted concurrently to determine mortality within three plant zones to evaluate chlorantraniliprole distribution throughout the canopy. For the leaf study, plants were partitioned into three zones consisting of a top (18th node), middle (13th node), and bottom (9th node) zone. Leaf samples from each zone were analyzed for chemical concentrations and bioassays were conducted at 1, 7, 14, 21, and 28 days after treatment (DAT). Floret samples were analyzed at 4, 7, 10, and 14 DAT. Concentrations of chlorantraniliprole, though variable, provided >71% control through all sampling dates, application rates, and canopy zones tested. Chlorantraniliprole was viable up to 28 DAT. Results from the soybean floret study suggested chlorantraniliprole was detected in florets up to 14 DAT. An additional leaf bioassay was conducted using concentrations detected in the floret study. Concentrations in florets provided mortality of corn earworm up to 48% out to 14 DAT. With a long residual expected, chlorantraniliprole applications should continue to be used to control corn earworm infestations in soybean and some additional control could be expected in florets.
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Glycine max , Mariposas Nocturnas , Animales , Glycine max/genética , ortoaminobenzoatos , Plantas Modificadas Genéticamente , Zea mays/genéticaRESUMEN
Studies of cost and value can inform educational decision making, yet our understanding of the barriers to such research is incomplete. To address this gap, our aim was to explore the attitudes of global thought leaders in HPE towards cost and value research. This was a qualitative virtual interview study underpinned by social constructionism. In telephone or videoconference interviews in 2018-2019, we asked global healthcare professional thought leaders their views regarding HPE cost and value research, outstanding research questions in this area and why addressing these questions was important. Analysis was inductive and thematic, and incorporated review and comments from the original interviewees (member checking). We interviewed 11 thought leaders, nine of whom gave later feedback on our data interpretation (member checking). We identified four themes: Cost research is really important but potentially risky (quantifying and reporting costs provides evidence for decision-making but could lead to increased accountability and loss of autonomy); I don't have the knowledge and skills (lack of economic literacy); it's not what I went into education research to do (professional identity); and it's difficult to generate generalizable findings (the importance of context). This study contributes to a wider conversation in the literature about cost and value research by bringing in the views of global HPE thought leaders. Our findings provide insight to inform how best to engage and empower educators and researchers in the processes of asking and answering meaningful, acceptable and relevant cost and value questions in HPE.
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Empleos en Salud , Humanos , Investigación Cualitativa , Empleos en Salud/educaciónRESUMEN
Winter annual cover crops can be planted before soybean in Mississippi for many agronomic reasons. Incorporating winter annual cover crops into soybean production changes the seasonal hosts within fields. Some studies suggest that reducing tillage and using diverse species of cover crops can increase arthropod diversity and predator activity. Neonicotinoid seed treatments are often implemented to combat early season insect pests in soybean that follow cover crops, but negative effects on the environment such as reductions in biodiversity are often attributed to these compounds. We conducted an experiment to measure the effects on the diversity of the soybean epigeal and foliar communities when incorporating cover crops as well as insecticidal seed treatments into Mississippi soybean growing systems. Our results showed that legume cover crops had significant impacts on the epigeal community diversity of soybean planted behind them. These cover crops, especially hairy vetch, supported a more diverse foliar community before termination. To prevent increases in herbivorous arthropods, neonicotinoid seed treatments can be used without affecting epigeal predators such as beetles, ants, and spiders. The neonicotinoid seed treatments affected arthropod diversity, but the reductions were mainly caused by decreases in herbivorous pest insects that fed on treated soybean plants.
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Artrópodos , Fabaceae , Animales , Productos Agrícolas , Insectos , Mississippi , Neonicotinoides , Estaciones del Año , Semillas , Glycine maxRESUMEN
Bt technologies have played a major role in the control of bollworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), in cotton. Variation in expression levels among varieties and plant parts, along with selection pressure on bollworm populations, has led to the development of resistance to some Bt proteins. Trials were conducted to evaluate how cotton varieties expressing different Bt proteins affect bollworm larval behavior and their damage in flowering cotton. Differences in larval recovery were observed among cotton varieties at 3 d with 3-gene Bt cotton having the lowest recovery and non-Bt cotton having the greatest recovery. Loss of bloom tags and abscission of small bolls at the site of infestation affected bollworm larval recovery among varieties. Day after infestation was the main factor that affected bollworm movement across all varieties. Number of total damaged fruiting forms by an individual bollworm larva was different among all varieties. Overall, flower bud (square) and fruit (boll) damage by an individual larva was lower on 3-gene cotton than 2-gene cotton and non-Bt cotton. An individual larva damaged fewer squares on 2-gene cotton than non-Bt cotton, but boll damage from bollworm was similar among 2-gene cotton and non-Bt cotton. The level of square and boll damage in 2-gene cotton has increased compared to previous research further supporting the occurrence of bollworm resistance to Cry proteins. The 3-gene cotton containing the Vip3A gene experienced low levels of damage and survival. These results will be important for improving management recommendations of bollworm in Bt cotton technologies.
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Proteínas Hemolisinas , Mariposas Nocturnas , Animales , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Endotoxinas/genética , Conducta Alimentaria , Frutas , Gossypium/genética , Proteínas Hemolisinas/metabolismo , Larva , Mariposas Nocturnas/genética , Control Biológico de Vectores/métodos , Plantas Modificadas Genéticamente/genéticaRESUMEN
Helicoverpa zea (Boddie), a pest of cotton that also occurs in field corn, is commonly controlled through the use of foliar-applied insecticides or transgenic crops expressing Bacillus thuringiensis (Berliner) (Bt) genes. To minimize the risk of Bt resistance in pest populations, refuge systems have been implemented for sustainable agroecosystem management. Historically, structured refuge compliance among growers has been low, leading to the commercialization of seed blended refugia. To test the viability of seed blended refugia in southern U.S. field corn, field studies were conducted in Mississippi and Georgia during 2016, 2017, and 2018 growing seasons. To quantify adult H. zea emergence from structured (non-Bt corn) and seed blended refuge options, emergence traps were utilized. Kernel damage among seed blended refuge and structured refuge corn ears were recorded and compared. The timing of moth emergence was recorded. When compared to a structured refuge, H. zea adult moth emergence from seed blended refugia did not significantly differ. Kernel damage of non-Bt plants in the seed blended treatments was not significantly different than non-Bt plants in the structured refuge treatments. Moth emergence timing was not significantly delayed between the structured refuge and seed blended refuge treatments. Results of this study suggest that a seed blended refuge may provide an effective insecticide resistance management alternative for H. zea in areas where structured refuge compliance is low.
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Bacillus thuringiensis , Mariposas Nocturnas , Animales , Bacillus thuringiensis/genética , Proteínas Bacterianas/genética , Endotoxinas , Proteínas Hemolisinas/genética , Resistencia a los Insecticidas , Mariposas Nocturnas/genética , Control Biológico de Vectores , Plantas Modificadas Genéticamente/genética , Refugio de Fauna , Semillas , Zea mays/genéticaRESUMEN
Water conservation is an important factor for production of rice in the United States because of declining aquifer levels, but little research has been done to evaluate insect management in rice systems integrating water conservation practices. Rice water weevil, Lissorhoptrus oryzophilus Kuschel, is an important insect pest of rice in the U.S. Rice water weevil is a semiaquatic species that relies on flooded conditions to complete larval development, so water conservation practices are likely to impact their pest status. The study was conducted across the Mississippi River alluvial floodplain to compare rice water weevil population densities in different zones of a furrow irrigated rice field to a conventionally flooded rice field. All locations were sampled at 3, 4, and 5 wk after the initial irrigation. Larval densities were greatest in the lower end of furrow irrigated fields and in the adjacent flooded rice field compared with the upper and middle sections that did not hold standing water when averaged across three sample dates. Also, rice water weevil densities were greater during week five than week three. In terms of rice yields, the top third of furrow irrigated rice fields, the section that remained mostly dry, produced lower rough rice yields than all other sections and the flooded field. These results suggest that rice water weevil populations can be lower in a furrow irrigated rice system. As a result, more research is needed to determine whether a spatial management plan can be developed based on soil moisture zones in furrow irrigated rice.
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Escarabajos , Oryza , Gorgojos , Animales , Mississippi , Suelo , AguaRESUMEN
Tarnished plant bug, Lygus lineolaris Palisot de Beauvois (Hemiptera: Miridae), has become a primary pest of cotton in the Midsouthern United States. Insect growth regulators such as novaluron are an important part of L. lineolaris management. While novaluron is lethal to nymphs, it does not kill adults, so it has been used when nymphs are the primary stage present. However, cotton yield protection was observed from an application of novaluron when adults were the predominant stage present. To explain this, a series of studies were conducted to examine sublethal impacts of novaluron to L. lineolaris adults. Novaluron ingestion by adults reduced hatch rate and sometimes reduced oviposition rate. Ingestion by either males or females reduced hatch rates, but the reduction was greater from female exposure. Contact exposure of adults with novaluron residues within 1 d of application reduced hatch rate by about 50%, but the impact on oviposition was inconsistent. A field study showed reduced hatch rate from contact exposure to mixed-age natural populations, but the overall net reproductive rate was not reduced. Surface exposure of eggs to novaluron did not reduce hatch rate. Overall, exposure of tarnished plant bug adults to novaluron, regardless of adult age or exposure route, reduced egg viability. However, the impact on oviposition rate and net reproductive rate varied with adult age and exposure route. This understanding of sublethal impacts of novaluron, in addition to lethal impacts on nymphs, should be considered when choosing application times to maximize effects on L. lineolaris populations.
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Hemípteros , Heterópteros , Animales , Femenino , Masculino , Ninfa , Oviposición , Compuestos de Fenilurea/farmacologíaRESUMEN
BACKGROUND: Physical therapy initiated early in an ICU stay may reduce functional deficits in critically ill patients; however, the association of frailty with outcomes in those receiving early in-ICU rehabilitation is unknown. OBJECTIVE: To estimate the association between frailty and 3 outcomes in patients enrolled in an ICU randomized clinical trial (RCT). DESIGN: Exploratory secondary analyses of the CYCLE pilot RCT (NCT02377830). SETTING: 7 Canadian ICUs. PARTICIPANTS: Previously ambulatory critically ill adults. INTERVENTION: Participants were randomized to early in-bed cycling plus routine physiotherapy versus early routine physiotherapy alone. MEASUREMENTS: Using regression analyses, we modelled the association between pre-hospital Clinical Frailty Scale (CFS) scores, Physical Function in ICU Test-scored (PFIT-s), muscle strength, and mortality at hospital discharge, adjusting for illness severity (APACHE II) and the randomized intervention. We explored the influence of imputing mean PFIT-s and strength scores for decedents, and with listwise deletion of decedents in a sensitivity analysis. RESULTS: Of 66 patients, 2 had missing data, 2 had incomplete data, and 21 died by hospital discharge. At hospital discharge for 66 patients, frailty was not associated with PFIT-s (mean difference (MD) [95% CI]=0.20, [-2.08, 2.74]) or muscle strength (1.96, [-12.6, 16.6]). A sensitivity analysis yielded consistent results. Frailty was also not associated with hospital mortality (odds ratio 0.91, [0.28 to 2.93]). CONCLUSION: We found no association between pre-hospital frailty, physical function, strength, or mortality at hospital discharge in critically ill patients enrolled in an early rehabilitation trial. Larger sample sizes are needed to further explore the association of frailty with these outcomes at hospital discharge.
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Fragilidad/diagnóstico , Unidades de Cuidados Intensivos , Fuerza Muscular/fisiología , Rehabilitación , Canadá , Enfermedad Crítica , Humanos , Respiración ArtificialRESUMEN
An experiment was conducted at the Delta Research and Extension Center in Stoneville, MS during 2017 and 2018 to determine whether removal of the flood is an economical method of control for rice water weevil, Lissorhoptrus oryzophilus Kuschel. This experiment compared a continuous flood production system to draining a rice field completely and reestablishing a flood for the remainder of the growing season. In addition, two insecticide seed treatments, thiamethoxam and chlorantraniliprole, were compared with an untreated control within each system. Rice water weevil densities were measured prior to draining at 3 wk after flood and again after the flood was reestablished in drained plots. Rice water weevil densities were greater in 2017 than 2018. Chlorantraniliprole at the predrainage and postdrainage sample timing reduced larval numbers compared with the untreated control. The plots where water was removed until soil cracking then re-flooded had significantly lower weevil populations than plots that were continuously flooded during 2018 only. Draining of plots resulted in lower yields in 2018, but not in 2017. Additionally, both of the insecticide seed treatments resulted in greater yields and economic returns than the untreated control. Draining of flooded rice when rice water weevil larvae were present did not provide a consistent benefit, and may result in yield and economic penalties. Insecticide seed treatments consistently provided greater yield benefits in flooded rice. Based on these results, draining of flooded rice is not recommended to manage rice water weevil and insecticide seed treatments should be used to minimize economic losses.
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Escarabajos , Insecticidas , Oryza , Gorgojos , Animales , Inundaciones , Control de Insectos , Larva , Semillas , AguaRESUMEN
The 5th Canadian Cancer Research Conference (ccrc) took place 3-5 November 2019 in Ottawa, Ontario. Nearly 1000 participants-scientists, oncologists, community members, and patients-gathered to share knowledge, foster collaboration, and fuel the future of cancer research in Canada. The scientific program included 3 plenary sessions, 26 concurrent sessions, and 2 poster sessions presenting research described in more than 600 submitted abstracts, giving participants the opportunity to share health research that collectively encompassed the 4 pillars recognized by the Canadian Institutes of Health Research. In addition to the breadth of topics addressed by Canadian and international experts, the highlights of the meeting included the integration of patients and patient advocates, new rapid-fire sessions for abstract presentation, and events that enhanced learning opportunities for trainees.
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Neoplasias/epidemiología , Canadá , Historia del Siglo XXI , HumanosRESUMEN
The tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), is an important pest of cotton in many areas of the southern United States. An experiment was conducted at two locations in Mississippi during 2016 and 2017 to evaluate action thresholds for tarnished plant bug on a novel Bacillus thuringiensis cotton that expresses the Cry51Aa2.834_16 toxin. Treatments included the current action threshold, a 2× threshold, and treatments where insecticides were only applied during the early season (preflower) or only during late season (during flowering) based on the current action thresholds. These were compared to an untreated control and a weekly insecticide use regime that received weekly insecticide sprays. All treatments were imposed on both Bt Cry1Aa2.834_16 cotton and a nontraited cotton. The Bt Cry1Aa2.834_16 trait reduced the number of tarnished plant bugs and injury, and improved yields compared to nontraited cotton. For all spray treatments except the weekly insecticide use regime, yields were greater for the Bt Cry51Aa2.834_16 cotton than the nontraited cotton. In terms of thresholds, Bt Cry1Aa2.834_16 cotton sprayed based on current action thresholds resulted in similar yields to the weekly insecticide use regime of both cotton types. In contrast, the 2× threshold resulted in lower yields than the current threshold for both cotton types. Though thresholds intermediate to the currently recommended action threshold and the 2× threshold were not tested, these data suggest that currently recommended action thresholds appear appropriate for Bt Cry51Aa2.834_16 cotton. These results suggest that this trait will be an important component of current IPM programs in cotton where tarnished plant bug is an important pest.
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Heterópteros , Insecticidas , Animales , Gossypium , Mississippi , Estaciones del AñoRESUMEN
Water hemlock (Cicuta douglasii) is one of the most toxic plants to livestock and humans. Little is known regarding the amount of plant required to cause death. The objective of this study was to determine a lethal dose of water hemlock in a goat model. Plants were dosed to goats via oral gavage of freeze-dried ground plant material. The results from this study suggest that 1-2 fresh tubers would be lethal to goats.
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Cicuta/toxicidad , Pruebas de Toxicidad Aguda , Animales , Cabras , Ganado , Modelos Animales , Intoxicación por Plantas/veterinaria , Plantas Tóxicas/toxicidadRESUMEN
OBJECTIVE: As ownership of brachycephalic dog breeds rises, the surgical correction of components of brachycephalic airway syndrome (BAS) is increasingly recommended by veterinarians. This study's objective was to describe the incidence of, and strategies for the management of post-operative respiratory complications in brachycephalic dogs undergoing surgical correction of one or more components of BAS. METHODS: Medical records of 248 brachycephalic dogs treated surgically for BAS were retrospectively reviewed for demographic information, procedures performed, post-operative complications and treatment implemented, hospitalisation time, and necessity for further surgery. RESULTS: Pugs, Cavalier King Charles Spaniels and British Bulldogs were the most commonly encountered breeds. Dogs which experienced a complication were significantly older (mean was 5.5 years, compared with 4.1 years [P < 0.01]). Fifty-eight dogs (23.4%) had complications which included: dyspnoea managed with supplemental oxygen alone (7.3%, n = 18), dyspnoea requiring anaesthesia and re-intubation (8.9%, n = 22), dyspnoea necessitating treatment with a temporary tracheostomy (8.9%, n = 22), aspiration pneumonia (4%, n = 10), and respiratory or cardiac arrest (2.4%, n = 6). Five of the 22 dogs requiring anaesthesia and re-intubation deteriorated 12 or more hours after post-surgical anaesthetic recovery. The overall mortality rate in this study was 2.4% (n = 6). Age, concurrent airway pathology, and emergency presentation significantly predicted post-operative complications. CONCLUSION: Our data show the importance of close monitoring for a minimum of 24 h following surgery by an experienced veterinarian or veterinary technician. Surgical intervention for BAS symptomatic dogs should be considered at an earlier age as an elective procedure, to reduce the risk of post-operative complications.
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Obstrucción de las Vías Aéreas/veterinaria , Craneosinostosis/veterinaria , Enfermedades de los Perros , Animales , Perros , Humanos , Complicaciones Posoperatorias/veterinaria , Periodo Posoperatorio , Estudios RetrospectivosRESUMEN
INTRODUCTION: Innate immune activation through exposure to indoor and outdoor pollutants is emerging as an important determinant of asthma severity. For example, household levels of the bacterial product lipopolysaccharide (LPS) are associated with increased asthma severity. We hypothesized that activation of the innate immune receptor TLR5 by its bacterial ligand flagellin will exacerbate airway inflammation and asthma symptoms. METHODS: We determined the effect of flagellin co-exposure with ovalbumin in a murine model of allergic asthma. We evaluated the presence of flagellin activity in house dust of asthma patients. Finally, we analyzed the association of a dominant-negative polymorphism in TLR5 (rs5744168) with asthma symptoms in patients with asthma. RESULTS: We showed that bacterial flagellin can be found in the house dust of patients with asthma and that this bacterial product exacerbates allergic airway inflammation in an allergen-specific mouse model of asthma. Furthermore, a dominant-negative genetic polymorphism in TLR5, the receptor for flagellin, is associated with decreased symptoms in patients with asthma. CONCLUSION: Together, our results reveal a novel genetic protective factor (TLR5 deficiency) and a novel environmental pollutant (microbial flagellin) that influence asthma severity. (Clinical trials NCT01688986 and NCT01087307).
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Asma/metabolismo , Hiperreactividad Bronquial/metabolismo , Broncoconstricción , Pulmón/metabolismo , Receptor Toll-Like 5/metabolismo , Adulto , Animales , Asma/inducido químicamente , Asma/inmunología , Asma/fisiopatología , Hiperreactividad Bronquial/inducido químicamente , Hiperreactividad Bronquial/inmunología , Hiperreactividad Bronquial/fisiopatología , Estudios de Casos y Controles , Estudios Transversales , Citocinas/metabolismo , Modelos Animales de Enfermedad , Femenino , Flagelina , Células HEK293 , Humanos , Pulmón/inmunología , Pulmón/fisiopatología , Masculino , Ratones Endogámicos C57BL , Persona de Mediana Edad , Ovalbúmina , Polimorfismo de Nucleótido Simple , Transducción de Señal , Células TH1/inmunología , Células TH1/metabolismo , Receptor Toll-Like 5/genéticaRESUMEN
The agricultural sector suffers from high risk of injury and damage to human health. There is considerable research not only identifying these risks but also finding ways to mitigate them. Beekeeping or apiculture, recognised as part of this sector, has many risk factors such as heavy lifting, high degree of manual materials handling, twisting, and awkward positioning common to all agriculture areas. It also has some unique risks such as those resulting from bee stings and smokers. However, there is much less attention focused on the health and safety of apiculture to the human beekeepers, and much more attention focused on bee health and safety. An ergonomics case study on beekeeping inspection tasks involving three independent, local beekeepers showed that many tasks involve awkward positions of the body, arms and hands, excessive lifting well beyond recommended weight limits, eye strain, and chemical and sting exposure. In addition, beekeepers are more interested in bee and hive health rather than reducing human-centred risk factors such as those due to excessive lifting. Standard ergonomics interventions such as a magnifier inspection and lift assist systems as well as interventions unique to beekeeping such as a smokeless method of calming bees are recommended. The beekeeping industry seems to have been forgotten in the modernisation of technology and agricultural practices. This paper offers some initial insights into possible points for research, development and improvements.
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Corn earworm, Helicoverpa zea (Boddie) Lepidoptera: Noctuidae, has not been considered an economic pest of field corn. Historical losses estimates ranged from 1.5 to 2.5%, and a large number of foliar insecticide applications would be needed to minimize infestations. In recent years, Bt, Bacillus thuringiensis (Berliner) field corn, Zea mays (L.) Poales: Poaceae, technologies that exhibit activity against corn earworm have been introduced. However, it is unclear how much damage to corn ears (number of damaged kernels) is required to reduce yield. In this study manual damage methods were utilized to inflict defined levels of kernel damage and to impose damage at levels greater than observed with natural corn earworm infestations. Bt corn hybrids expressing the Agrisure Viptera (Vip 3A) trait were used to minimize injury from natural infestations of corn earworm. Manual kernel damage was imposed at R3 stage to mimic corn earworm feeding while avoiding interference with pollination. These methods were used in experiments where treatments were applied to individual ears and hand-harvested and in experiments where treatments were applied to all primary ears in the plot and machine-harvested. Damage of ≥60 kernels per ear was required to significantly reduce yield regardless of harvest method. Kernel damage from natural corn earworm infestations reported in other studies was much lower than 60 kernels per ear. Timely planting is a key component of all integrated pest management programs. Field corn planted during the recommended planting window for optimum yield is unlikely to experience corn earworm damage great enough to reduce yield.
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Bacillus thuringiensis , Mariposas Nocturnas , Animales , Proteínas Bacterianas , Endotoxinas , Proteínas Hemolisinas , Larva , Control Biológico de Vectores , Plantas Modificadas Genéticamente , Zea maysRESUMEN
Variations in the SLC45A2 gene are responsible for the dilution phenotypes cream and pearl in domestic horses. Cream dilution is inherited in an incomplete dominant manner, diluting only red in the heterozygous state but both red and black pigments when two alleles are present. The pearl dilution is recessive and dilutes only the red and black pigment in the homozygous state or when paired with a cream allele. Horses that inherit one copy of pearl (Cprl ) and one copy of the dominant cream allele (CC r ) display a dilution phenotype similar to that of homozygous cream, suggesting that pearl is the result of a different variation in the same gene responsible for cream. We sequenced SLC45A2 in two 'false double dilute' horses that appeared phenotypically homozygous cream but tested as possessing only a single CC r allele. We also sequenced one known pearl carrier to screen for putative causal variants. The missense variant ECA21:SLC45A2:c.985G>A; p.Ala329Thr (Cprl ) was present in one false double dilute and the pearl carrier and was also genotyped in an additional 126 horses for statistical evaluation. The genotype matched the expected phenotype in all horses (P-value = 6.5 × 10-41 ) and is identical to a pearl variant found previously. The second false double dilute horse and one non-dilute offspring genotyped as heterozygous for a novel missense variant ECA21:SLC45A2:c.568G>A (p.Gly190Arg), the proposed Csun variant (for the name of the horse). This variant produces a recessive dilution similar to pearl and indicates that multiple alleles of SLC45A2 result in dilution phenotypes in the domestic horse.
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Color del Cabello , Caballos/genética , Proteínas de Transporte de Membrana/genética , Animales , Heterocigoto , PigmentaciónRESUMEN
Healthcare and health professions education share many of the same problems in decision making. In both cases, there is a finite amount of resources, and so choices need to be made between alternatives. To navigate the options available requires effective decision making. Choosing one option requires consideration of its opportunity cost - the benefit forgone of the other competing options. The purpose of this abridged AMEE guide is to introduce educational decision-makers to the economic concept of cost, and how to read studies about educational costs to inform effective cost-conscious decision-making. This guide leads with a brief review of study designs commonly utilized in this field of research, followed by an overview of how study findings are commonly presented. The tutorial will then offer a four-step model for appraising and considering the results of an economic evaluation. It asks the questions: (1) Can I trust the results? (2) What are the results telling me? (3) Could the results be transferred to my context? (4) Should I change my practice?