RESUMEN
Two groups of children were set up: children infected with HIV and hepatitis viruses and children infected with HIV, but not with hepatitis viruses, too. The immunological aspects investigated referred to the Ig serum value, the absolute number of T CD4 lymphocytes and the T CD4/T CD8 ratio. The subjects of the first group (in whom hepatitis markers were present) displayed increased IgA and IgM values at a higher rate than those of the second group (54.87% against 32%, 83.3% against 53%), as well as a lowering below 300/ml of the number of T CD4 lymphocytes (50% against 29.4%). On the other hand, increased IgG levels and values below 0.8 of the T CD4/T CD8 ratio were found at similar rates in the two groups of children (77.4% against 80% and 70.5% against 70% respectively). By means of the data obtained, the authors try to point out one of the ways by which hepatitis viruses, considered as a potential cofactor in the AIDS development, contribute to the course of this disease, namely by intensifying the immunological disorders.
Asunto(s)
Infecciones Oportunistas Relacionadas con el SIDA/inmunología , Hepatitis B/complicaciones , Hepatitis C/complicaciones , Infecciones Oportunistas Relacionadas con el SIDA/sangre , Infecciones Oportunistas Relacionadas con el SIDA/virología , Recuento de Linfocito CD4 , Relación CD4-CD8 , Niño , Preescolar , Femenino , Estudios de Seguimiento , Hepatitis B/inmunología , Hepatitis B/virología , Antígenos de Superficie de la Hepatitis B/sangre , Hepatitis C/inmunología , Hepatitis C/virología , Anticuerpos contra la Hepatitis C/sangre , Humanos , Lactante , MasculinoAsunto(s)
Anticuerpos Antibacterianos/sangre , Pruebas de Fijación del Complemento/métodos , Técnicas para Inmunoenzimas , Mycoplasma pneumoniae/inmunología , Enfermedad Aguda , Pruebas de Fijación del Complemento/estadística & datos numéricos , Estudios de Evaluación como Asunto , Humanos , Técnicas para Inmunoenzimas/estadística & datos numéricos , Neumonía por Mycoplasma/diagnósticoRESUMEN
Complex serological survey performed in the town B. during the first half of 1992 emphasized an active circulation of some respiratory viruses, especially of parainfluenza type 3 and 1, influenza subtypes A(H3N2) and A(H1N1), adeno and syncytial respiratory as well as of Mycoplasma pneumoniae. Less important was the frequence of antibodies against Chlamydia psittaci, East equine, West Nile and tick-borne encephalitis as well as parainfluenza type 2 and influenza type B viruses.
Asunto(s)
Población Urbana , Virus/inmunología , Adolescente , Adulto , Anticuerpos Antibacterianos/sangre , Anticuerpos Antivirales/sangre , Niño , Preescolar , Chlamydophila psittaci/inmunología , Pruebas de Fijación del Complemento , Pruebas de Inhibición de Hemaglutinación , Humanos , Lactante , Recién Nacido , Persona de Mediana Edad , Neumonía por Mycoplasma/inmunología , RumaníaRESUMEN
A study was conducted on 61 polytransfused patients, 24 patients submitted to long lasting parenteral treatments, 116 blood donors and 132 controls. Immuno-enzymatic tests were used for detection of antibodies against hepatitis C virus (HCV). They were found in 56 (91.8%) out of the 61 polytransfused patients, in 5 (4.3%) of the donors, in 5 (20.8%) of the patients with multiple treatments and in 5 (3.7%) controls. The VHB markers were detected at variable rates in all investigated groups. The high rate of seropositivity indicates that hepatitis C is becoming an important public health problem in Romania.
Asunto(s)
Transfusión Sanguínea/estadística & datos numéricos , Hepacivirus/inmunología , Anticuerpos Antihepatitis/sangre , Hepatitis C/epidemiología , Adulto , Donantes de Sangre/estadística & datos numéricos , Niño , Hemofilia A/inmunología , Hemofilia A/terapia , Hepatitis C/inmunología , Humanos , Prevalencia , Factores de Riesgo , Rumanía/epidemiología , Estudios Seroepidemiológicos , Talasemia beta/inmunología , Talasemia beta/terapiaRESUMEN
A study was conducted on 61 polytransfused patients, 24 patients submitted to long lasting parenteral treatments, 116 blood donors and 132 controls. Immuno-enzymatic tests were used for detection of antibodies against hepatitis C virus (HCV). They were found in 56 (91.8%) out of the 61 polytransfused patients, in 5 (4.3%) of the donors, in 5 (20.8%) of the patients with multiple treatments and in 5 (3.7%) controls. The VHB markers were detected at variable rates in all investigated groups. The high rate of seropositivity indicates that hepatitis C is becoming an important public health problem in Romania.
Asunto(s)
Anticuerpos contra la Hepatitis C/sangre , Hepatitis C/epidemiología , Reacción a la Transfusión , Adulto , Biomarcadores , Donantes de Sangre , Niño , Hepatitis B/epidemiología , Anticuerpos contra la Hepatitis B/sangre , Antígenos de la Hepatitis B/sangre , Hepatitis C/transmisión , Humanos , Incidencia , Complicaciones Posoperatorias/epidemiología , Complicaciones Posoperatorias/virología , Rumanía/epidemiología , Estudios Seroepidemiológicos , Talasemia/complicaciones , Talasemia/terapiaRESUMEN
By a qualitative analysis of the solutions of the mathematical model equations (describing the morbidity and susceptibility evolution in a viral epidemics), approximate formulae for the extreme values of the variables and for the duration of the main phases of a multiannual cycle are deduced. These formulae were validated by numerical simulation of the solutions, leading to the exact values of the mentioned essential characteristics of the diseases propagation.
Asunto(s)
Brotes de Enfermedades , Modelos Biológicos , Virosis/epidemiología , Brotes de Enfermedades/estadística & datos numéricos , Susceptibilidad a Enfermedades , Métodos Epidemiológicos , Humanos , Matemática , Rumanía/epidemiología , Virosis/transmisiónRESUMEN
A study was worked out on the etiopathogenic relations and a more exact evaluation of the incidence of some agents: viruses, chlamydiae, mycoplasmas and rickettsiae, in the respiratory diseases of 0- to 12-month-old children. Serological analysis and immunofluorescence examination of pharyngeal cells were done. Results showed that parainfluenzae and respiratory syncytial viruses, chlamydiae, rickettsiae and mycoplasmas had an important part in the outbreak and maintaining of severe respiratory diseases in the 0- to 12-month-old children population.
Asunto(s)
Infecciones del Sistema Respiratorio/microbiología , Anticuerpos Antibacterianos/análisis , Anticuerpos Antivirales/análisis , Antígenos Bacterianos/análisis , Antígenos Virales/análisis , Femenino , Humanos , Lactante , Recién Nacido , Masculino , Faringe/inmunología , Faringe/microbiología , Infecciones del Sistema Respiratorio/epidemiología , Infecciones del Sistema Respiratorio/etiología , Infecciones del Sistema Respiratorio/inmunología , Rumanía/epidemiologíaRESUMEN
A group of 55 patients with insulin-dependent diabetes mellitus, aged from 8 to 40 years, was followed and compared to a group of controls, in view of detecting a possible relation between the presence of inframicrobial agents and diabetes pathogenesis. The investigations revealed, in patients, significantly higher positivity rate of antibodies against mumps (27% versus 16.3% in controls), parainfluenza type 1 (45.4% versus 29.1%) and Epstein-Barr (54.5% versus 10.9%) viruses, and Mycoplasma pneumoniae (43.6 versus 18.2%).
Asunto(s)
Diabetes Mellitus Tipo 1/microbiología , Adolescente , Adulto , Anticuerpos Antibacterianos/sangre , Anticuerpos Antivirales/sangre , Bacterias/inmunología , Bacterias/aislamiento & purificación , Niño , Diabetes Mellitus Tipo 1/etiología , Diabetes Mellitus Tipo 1/inmunología , Humanos , Faringe/microbiología , Estaciones del Año , Virus/inmunología , Virus/aislamiento & purificaciónRESUMEN
This work presents the results of a study on some viral, chlamydial, rickettsial and mycoplasmal agents incidence and their role on several gynecological ailments: vulvovaginitis, cervicitis, cervicovaginitis. The presence of some viral, chlamydial, rickettsial and mycoplasmal antigens in the vaginal secretion was investigated by immunofluorescence technique, isolation on selective media for Mycoplasma, and MGG staining in a group of 60 women with gynecopathies (group A) and in a control group (also 60 clinically healthy women) (group B). This study pointed out to higher incidence of chlamydial and mycoplasma antigens as well as of parainfluenza virus types I and III, adenovirus and herpes virus type II in group A. The serological screening revealed significant differences between the specific antibodies to antigens found in vaginal secretions to prove the presence of an immunological response on the same category of subjects.
Asunto(s)
Infecciones por Chlamydia/complicaciones , Enfermedades de los Genitales Femeninos/etiología , Infecciones por Mycoplasma/complicaciones , Infecciones por Rickettsia/complicaciones , Virosis/complicaciones , Anticuerpos Antibacterianos/análisis , Anticuerpos Antivirales/análisis , Chlamydia/inmunología , Chlamydia/aislamiento & purificación , Infecciones por Chlamydia/diagnóstico , Femenino , Enfermedades de los Genitales Femeninos/diagnóstico , Humanos , Mycoplasma/inmunología , Mycoplasma/aislamiento & purificación , Infecciones por Mycoplasma/diagnóstico , Rickettsia/inmunología , Rickettsia/aislamiento & purificación , Infecciones por Rickettsia/diagnóstico , Cervicitis Uterina/diagnóstico , Cervicitis Uterina/etiología , Vagina/inmunología , Vagina/microbiología , Virosis/diagnóstico , Virus/inmunología , Virus/aislamiento & purificación , Vulvovaginitis/diagnóstico , Vulvovaginitis/etiologíaRESUMEN
Essential peculiarities of the kinetics of the morbidity evolution of most frequent virus diseases were explained using a determinant mathematical model. The main epidemiological parameters for the whole territory of the S.R. of Romania and of Sibiu County were evaluated for the 1967-1986 period.
Asunto(s)
Virosis/epidemiología , Métodos Epidemiológicos , Humanos , Matemática , Modelos Biológicos , RumaníaRESUMEN
The report presents a statistical synthesis (a microcomputer Junior was used) concerning the incidence of various acute infections of the upper respiratory tract (AIURT), the stages of their evolution, the distribution of cases among the population groups, the seasonal incidence and the values of epidemiologic parameters. We used clinical and epidemiologic data on AIURT of viral etiology (especially influenza). The structure of a data base on the epidemiology of AIURT of viral etiology is also presented.
Asunto(s)
Gripe Humana/epidemiología , Absentismo , Factores de Edad , Procesamiento Automatizado de Datos , Humanos , Gripe Humana/complicaciones , Gripe Humana/mortalidad , Estudios Prospectivos , Estudios Retrospectivos , Rumanía , Estaciones del AñoRESUMEN
Investigations were conducted on groups of subjects hospitalized in surgery services with a clinical diagnosis of acute or chronic appendicitis, to detect the presence of inframicrobial antibodies and antigens, as well as that of the C reactive protein in these patients as compared with a control group. The results of serological tests and of the examination of pieces of appendix are also presented. The obtained data are used as arguments for the theory of an inframicrobial infection part in some acute and chronic forms of appendicitis.
Asunto(s)
Apendicitis/inmunología , Virosis/inmunología , Adolescente , Adulto , Anticuerpos Antivirales/análisis , Antígenos Virales/análisis , Apéndice/inmunología , Proteína C-Reactiva/análisis , Humanos , Técnicas InmunológicasRESUMEN
Investigations conducted on subjects with epidemic parotitis serologically confirmed (hemagglutination inhibition) allowed the detection of amounts of C reactive protein (Crp) higher than the normal level in 34% of the serum samples collected during the first twelve days of illness. After that, the Crp gradually decreased. The results showed that Crp is present in more than 40% of the subjects with uncomplicated mumps and in 55 to 75% of the cases with such complications as meningitis or orchitis. In most of the subjects, the Crp was found only in the first sample, but not in the second. Nevertheless, high amounts were found later, simultaneously with anti-mumps antibodies.
Asunto(s)
Proteína C-Reactiva/metabolismo , Paperas/sangre , Pruebas de Inhibición de Hemaglutinación , Humanos , Factores de TiempoRESUMEN
A mathematical methodology is suggested which allows the a priori calculation of the risk of premature delivery, by a formula relying on the scores attributed to different specific risk factors (maternal virus infections included). The scores are estimated either directly, or starting from the scores of nonspecific factors. The statistical significance of the different scores, correlations and formulas is analyzed.
Asunto(s)
Recien Nacido Prematuro , Complicaciones del Embarazo , Virosis/complicaciones , Adulto , Femenino , Humanos , Recién Nacido , Trabajo de Parto Prematuro , Embarazo , Complicaciones Infecciosas del Embarazo , RiesgoRESUMEN
Various techniques are described for the preparation of complement-fixing(CF) antigen from the cell-associated phase of two strains of human cytomegalovirus. Preliminary results regarding the preparation of CF antigen from extracellular virus are also presented. The qualities and shortcomings of each of the biological preparations obtained by the different procedures are outlined.
Asunto(s)
Antígenos Virales/aislamiento & purificación , Citomegalovirus/inmunología , Antígenos Virales/inmunología , Línea Celular , Pruebas de Fijación del Complemento , Citomegalovirus/crecimiento & desarrollo , Humanos , Concentración de Iones de Hidrógeno , Temperatura , UltrasonidoRESUMEN
The authors discuss different aspects of the rapid diagnosis of viral infections: its necessity, its characteristics, the recent advances having allowed the development of modern techniques. Further, the main categories of procedures used in rapid viral diagnosis are reviewed: 1) electron microscopy, 2) immunofluorescence and immunoperoxidase techniques; 3) solid-phase systems with labelled reactants; 4) agglutination with supporting particles; 5) counterimmunoelectrophoresis; 6) rapid/early serological diagnosis. Data are discussed as regards the possibility of the large-scale application of rapid viral diagnosis procedures in Romania.