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1.
Braz J Med Biol Res ; 35(3): 297-303, 2002 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-11887207

RESUMEN

We report the detection of insulin-like antigens in a large range of species utilizing a modified ELISA plate assay and Western blotting. We tested the leaves or aerial parts of species of Rhodophyta (red alga), Bryophyta (mosses), Psilophyta (whisk ferns), Lycopodophyta (club mosses), Sphenopsida (horsetails), gymnosperms, and angiosperms, including monocots and dicots. We also studied species of fungi and a cyanobacterium, Spirulina maxima. The wide distribution of insulin-like antigens, which in some cases present the same electrophoretic mobility as bovine insulin, together with results recently published by us on the amino acid sequence of an insulin isolated from the seed coat of jack bean (Canavalia ensiformis) and from the developing fruits of cowpea (Vigna unguiculata), suggests that pathways depending on this hormone have been conserved through evolution.


Asunto(s)
Hongos/química , Insulina/análisis , Proteínas de Plantas/análisis , Proteínas/análisis , Rhodophyta/química , Animales , Proteínas Bacterianas/análisis , Proteínas Bacterianas/genética , Western Blotting , Bovinos , Cianobacterias/química , Electroforesis en Gel de Poliacrilamida , Ensayo de Inmunoadsorción Enzimática , Hongos/genética , Peso Molecular , Proteínas de Plantas/genética , Rhodophyta/genética
2.
Braz. j. med. biol. res ; 35(3): 297-303, Mar. 2002. ilus, tab
Artículo en Inglés | LILACS | ID: lil-304679

RESUMEN

We report the detection of insulin-like antigens in a large range of species utilizing a modified ELISA plate assay and Western blotting. We tested the leaves or aerial parts of species of Rhodophyta (red alga), Bryophyta (mosses), Psilophyta (whisk ferns), Lycopodophyta (club mosses), Sphenopsida (horsetails), gymnosperms, and angiosperms, including monocots and dicots. We also studied species of fungi and a cyanobacterium, Spirulina maxima. The wide distribution of insulin-like antigens, which in some cases present the same electrophoretic mobility as bovine insulin, together with results recently published by us on the amino acid sequence of an insulin isolated from the seed coat of jack bean (Canavalia ensiformis) and from the developing fruits of cowpea (Vigna unguiculata), suggests that pathways depending on this hormone have been conserved through evolution


Asunto(s)
Animales , Bovinos , Hongos , Insulina , Proteínas de Plantas , Proteínas Proto-Oncogénicas c-bcl-2 , Rhodophyta , Proteínas Bacterianas , Western Blotting , Electroforesis en Gel de Poliacrilamida , Ensayo de Inmunoadsorción Enzimática , Hongos , Peso Molecular , Proteínas de Plantas , Rhodophyta
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